1b:I[42426,["/_next/static/immutable/chunks/1xctgzqre-wsy.js","/_next/static/immutable/chunks/06_-wz6pdtp2b.js","/_next/static/immutable/chunks/30e52g49hg40p.js","/_next/static/immutable/chunks/02yq6tul2z-oh.js","/_next/static/immutable/chunks/10bx_ec2vj7n_.js","/_next/static/immutable/chunks/33bfn03fo8xnt.js","/_next/static/immutable/chunks/0tfjf78scu61b.js","/_next/static/immutable/chunks/31xnvej4n1bi9.js"],"default"] 1c:T46a,Plate readers (also known as microplate readers) are laboratory instruments designed to detect biological, chemical or physical events of samples in microtiter plates. They are widely used in research, drug discovery, bioassay validation, quality control and manufacturing processes in the pharmaceutical and biotechnological industry and academic organizations. Sample reactions can be assayed in 6-1536 well format microtiter plates. The most common microplate format used in academic research laboratories or clinical diagnostic laboratories is 96-well (8 by 12 matrix) with a typical reaction volume between 100 and 200 uL per well. Higher density microplates (384- or 1536-well microplates) are typically used for screening applications, when throughput (number of samples per day processed) and assay cost per sample become critical parameters, with a typical assay volume between 5 and 50 µL per well. Common detection modes for microplate assays are absorbance, fluorescence intensity, luminescence, time-resolved fluorescence, and fluorescence polarization. From: http://en.wikipedia.org/wiki/Plate_reader, 2014-09-0-23.1d:Tae9,This project will investigate feeding by copepod nauplius larvae, the most abundant metazoans in the sea. It will answer three questions: 1) How does food selection by adults and nauplii differ when they are fed multiple prey species in the laboratory? 2) How does food selection by adults and nauplii differ when they are feeding on natural prey assemblages? and, 3) How do growth, development, and survival differ between copepodites and nauplii when their growth is food limited? Comparative experiments and field-based measurements will contrast the food consumed, and the effects of food limitation, between nauplii and later life stages. This contrast will include attributes of food such as size, taxon, and motility, and will include experiments with cultured prey offered singly or in a mixture, and natural prey, and apply genetic techniques to determine prey consumption by a predatory copepod. Copepods will be collected from the San Francisco Estuary, with four species selected for experiments to span taxonomic groups, sizes, salinity ranges, and general feeding behavior. A variety of techniques will be applied to account for the inevitable biases and limitations of each; all but one have previously been applied in our laboratories. These will include laboratory feeding experiments using cultured prey individually and in mixtures, and experiments using natural prey. Consumption of prey in experimental bottles will be measured as chlorophyll concentration and through particle counts by microscopy and flow cytometry. Radioactively labeled prey will be used in short incubations to determine feeding on particular prey types. Samples from the field will be examined for gut fluorescence. Separate experiments will determine how nauplii and copepodites survive and grow at different concentrations of food. Investigations of feeding by a predatory copepod (Tortanus dextrilobatus) will use molecular techniques to identify mitochondrial and nuclear DNA from diverse suspected prey species. Specific primers will be developed for common zooplankton species consumed by T. dextrilobatus in the laboratory. General primers and screening protocols developed here will be useful for identifying food web interactions in other estuarine communities. Copepod nauplii are important both in their diverse trophic roles in ocean foodwebs and in the population dynamics of copepods. Nauplii have a completely different feeding apparatus from later stages, and the first feeding stage can be very sensitive to starvation, making these life stages critical to population dynamics. Yet extant copepod population models treat nauplii as miniature adults. This work will provide valuable input to the growing efforts at modeling ocean ecosystems. Further details from final report (pdf)6:["$","$L1b",null,{"dataset":{"_id":"546486","description":"

We examined whether sonication was necessary for efficient extraction of chlorophyll (Mackas and Bohrer 1976).

Related Reference:

Vogt, R.A., T.R. Ignoffo, L.J. Sullivan, J. Herndon, J.H. Stillman, and W. Kimmerer. 2013. Feeding capabilities and limitations in the nauplii of two pelagic estuarine copepods, Pseudodiaptomus marinus and Oithona davisae. Limnology and Oceanography 58: 2145-2157.

\n","problem":"","acquisition":"

Twenty-seven adult female Pseudodiaptomus marinus were allowed to depurate for 3 h, then incubated with 500 µg C L-1 of Tetraselmis suecica for 30 min. Samples were prepared for gut pigment analysis as in Vogt (2013). Nine samples were sonicated using a Virtis© VirSonic 100 ultrasonic probe with the dial set to medium, using five 1 second pulses. Nine samples were daubed briefly with the end of the probe but not sonicated, to control for possible loss of liquid to the probe, and the remaining nine were not sonicated. Fluorescence measured as in Vogt (2013) showed no difference among treatments (ANOVA, p=0.5), so we extracted all samples without sonication.

\n","processing":"

Raw data was plotted to determine gut fluorescence in raw units, subtracting background fluorescence from copepods with empty guts (background).

BCO-DMO Processing:
\n- added conventional header with dataset name, PI name, version date, reference information
\n- renamed parameters to BCO-DMO standard

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replicate number

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"}},{"_id":"546500","name":"fluor_sonic","format":"","frictionlessType":"","description":"

fluorescence of sonicated samples

","units":{"_id":"","name":"relative fluorescence units"},"type":{"_id":"1900","name":"fluorescence counts","description":"

Output of a WetLabs fluorometer passed through a MetraByte A/D converter to convert the 0-5 volt dc fluorometer output to serial (count) data. The MetraByte device sets the output decimal point as necessary for best resolution, which results in a 1 volt dc fluorometer value being represented as +01000.00 in the raw MetraByte serial stream. (from Calliope metadata file KN207-01)

"}},{"_id":"546501","name":"fluor_probe","format":"","frictionlessType":"","description":"

fluorescence of samples manually daubed with sonicator probe but not sonicated

","units":{"_id":"","name":"relative fluorescence units"},"type":{"_id":"1900","name":"fluorescence counts","description":"

"}},{"_id":"546502","name":"fluor_no_soni","format":"","frictionlessType":"","description":"

fluorescence of non-sonicated samples

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"}},{"_id":"546503","name":"fluor_blank","format":"","frictionlessType":"","description":"

fluorescence of blank control samples

","units":{"_id":"","name":"relative fluorescence units"},"type":{"_id":"1900","name":"fluorescence counts","description":"

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