19:I[42426,["/_next/static/immutable/chunks/1xctgzqre-wsy.js","/_next/static/immutable/chunks/06_-wz6pdtp2b.js","/_next/static/immutable/chunks/30e52g49hg40p.js","/_next/static/immutable/chunks/02yq6tul2z-oh.js","/_next/static/immutable/chunks/10bx_ec2vj7n_.js","/_next/static/immutable/chunks/33bfn03fo8xnt.js","/_next/static/immutable/chunks/0tfjf78scu61b.js","/_next/static/immutable/chunks/31xnvej4n1bi9.js"],"default"] 1a:T46a,Plate readers (also known as microplate readers) are laboratory instruments designed to detect biological, chemical or physical events of samples in microtiter plates. They are widely used in research, drug discovery, bioassay validation, quality control and manufacturing processes in the pharmaceutical and biotechnological industry and academic organizations. Sample reactions can be assayed in 6-1536 well format microtiter plates. The most common microplate format used in academic research laboratories or clinical diagnostic laboratories is 96-well (8 by 12 matrix) with a typical reaction volume between 100 and 200 uL per well. Higher density microplates (384- or 1536-well microplates) are typically used for screening applications, when throughput (number of samples per day processed) and assay cost per sample become critical parameters, with a typical assay volume between 5 and 50 µL per well. Common detection modes for microplate assays are absorbance, fluorescence intensity, luminescence, time-resolved fluorescence, and fluorescence polarization. From: http://en.wikipedia.org/wiki/Plate_reader, 2014-09-0-23.1b:Tae9,This project will investigate feeding by copepod nauplius larvae, the most abundant metazoans in the sea. It will answer three questions: 1) How does food selection by adults and nauplii differ when they are fed multiple prey species in the laboratory? 2) How does food selection by adults and nauplii differ when they are feeding on natural prey assemblages? and, 3) How do growth, development, and survival differ between copepodites and nauplii when their growth is food limited? Comparative experiments and field-based measurements will contrast the food consumed, and the effects of food limitation, between nauplii and later life stages. This contrast will include attributes of food such as size, taxon, and motility, and will include experiments with cultured prey offered singly or in a mixture, and natural prey, and apply genetic techniques to determine prey consumption by a predatory copepod. Copepods will be collected from the San Francisco Estuary, with four species selected for experiments to span taxonomic groups, sizes, salinity ranges, and general feeding behavior. A variety of techniques will be applied to account for the inevitable biases and limitations of each; all but one have previously been applied in our laboratories. These will include laboratory feeding experiments using cultured prey individually and in mixtures, and experiments using natural prey. Consumption of prey in experimental bottles will be measured as chlorophyll concentration and through particle counts by microscopy and flow cytometry. Radioactively labeled prey will be used in short incubations to determine feeding on particular prey types. Samples from the field will be examined for gut fluorescence. Separate experiments will determine how nauplii and copepodites survive and grow at different concentrations of food. Investigations of feeding by a predatory copepod (Tortanus dextrilobatus) will use molecular techniques to identify mitochondrial and nuclear DNA from diverse suspected prey species. Specific primers will be developed for common zooplankton species consumed by T. dextrilobatus in the laboratory. General primers and screening protocols developed here will be useful for identifying food web interactions in other estuarine communities. Copepod nauplii are important both in their diverse trophic roles in ocean foodwebs and in the population dynamics of copepods. Nauplii have a completely different feeding apparatus from later stages, and the first feeding stage can be very sensitive to starvation, making these life stages critical to population dynamics. Yet extant copepod population models treat nauplii as miniature adults. This work will provide valuable input to the growing efforts at modeling ocean ecosystems. Further details from final report (pdf)6:["$","$L19",null,{"dataset":{"_id":"546506","description":"

We determined the minimum time necessary for copepods to evacuate their guts.

Related Reference:

Vogt, R.A., T.R. Ignoffo, L.J. Sullivan, J. Herndon, J.H. Stillman, and W. Kimmerer. 2013. Feeding capabilities and limitations in the nauplii of two pelagic estuarine copepods, Pseudodiaptomus marinus and Oithona davisae. Limnology and Oceanography 58: 2145-2157.

\n","problem":"","acquisition":"

Copepods may have elevated feeding rates after being starved for as little as 6 h before they are provided with food (Tiselius 1998). Groups of 10 adult female P. marinus were sorted from laboratory cultures and pipetted into 500 mL Nalgene bottles containing GF/F filtered seawater, maintained at 19°C in a constant temperature room. Copepods were sampled at 10 min intervals up to 180 min, and their extracts were measured on the microplate reader as described in Vogt (2013). Most of the gut pigment was depurated within the first 30 min of incubation in filtered water, and no further depuration occurred after 60 min. Three hour starvation periods were chosen to account for potential differences in individual variability in depuration rate.

\n","processing":"

Raw data was plotted to determine gut fluorescence in raw units, subtracting background fluorescence from copepods with empty guts (background).

BCO-DMO Processing:
\n- added conventional header with dataset name, PI name, version date, reference information
\n- renamed parameters to BCO-DMO standard

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"}},{"_id":"546518","name":"fluorescence","format":"","frictionlessType":"","description":"

fluorescence value

","units":{"_id":"","name":"relative fluorescence units??"},"type":{"_id":"1900","name":"fluorescence counts","description":"

Output of a WetLabs fluorometer passed through a MetraByte A/D converter to convert the 0-5 volt dc fluorometer output to serial (count) data. The MetraByte device sets the output decimal point as necessary for best resolution, which results in a 1 volt dc fluorometer value being represented as +01000.00 in the raw MetraByte serial stream. (from Calliope metadata file KN207-01)

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