http://lod.bco-dmo.org/id/dataset/546506
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2015-02-02
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Experimental results describing copepod gut evacuation time from species collected in the San Francisco estuary during 2013
2015-01-15
publication
2015-01-15
revision
BCO-DMO Linked Data URI
2015-01-15
creation
http://lod.bco-dmo.org/id/dataset/546506
William Kimmerer
San Francisco State University
principalInvestigator
Sarah Cohen
San Francisco State University
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Kimmerer, W., Cohen, S. (2015) Experimental results describing copepod gut evacuation time from species collected in the San Francisco estuary during 2013. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 2015-01-15) Version Date 2015-01-15 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/546506 [access date]
Experiment to test copepod gut evacuation time Dataset Description: <p>We determined the minimum time necessary for copepods to evacuate their guts.</p>
<p><strong>Related Reference:</strong></p>
<p>Vogt, R.A., T.R. Ignoffo, L.J. Sullivan, J. Herndon, J.H. Stillman, and W. Kimmerer. 2013. Feeding capabilities and limitations in the nauplii of two pelagic estuarine copepods, <em>Pseudodiaptomus marinus</em> and <em>Oithona davisae</em>.&nbsp; Limnology and Oceanography 58: 2145-2157.</p> Methods and Sampling: <p>Copepods may have elevated feeding rates after being starved for as little as 6 h before they are provided with food (Tiselius 1998). Groups of 10 adult female <em>P. marinus</em> were sorted from laboratory cultures and pipetted into 500 mL Nalgene bottles containing GF/F filtered seawater, maintained at 19°C in a constant temperature room. Copepods were sampled at 10 min intervals up to 180 min, and their extracts were measured on the microplate reader as described in Vogt (2013). Most of the gut pigment was depurated within the first 30 min of incubation in filtered water, and no further depuration occurred after 60 min. Three hour starvation periods were chosen to account for potential differences in individual variability in depuration rate.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-0929075 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=0929075
completed
William Kimmerer
San Francisco State University
(415) 338-3515
San Francisco State University / Romberg Tiburon Center 3152 Paradise Drive
Tiburon
CA
94920
kimmerer@sfsu.edu
pointOfContact
Sarah Cohen
San Francisco State University
415-338-3750
Romberg Tiburon Center 3152 Paradise Drive
Tiburon
CA
94920
USA
sarahcoh@sfsu.edu
pointOfContact
asNeeded
Dataset Version: 2015-01-15
Unknown
replicate
time
fluorescence
Fluorometer
Spectrophotometer
theme
None, User defined
replicate
time_elapsed
fluorescence counts
featureType
BCO-DMO Standard Parameters
Fluorometer
plate reader
Spectrophotometer
instrument
BCO-DMO Standard Instruments
Kimmerer_2013
service
Deployment Activity
San Francisco State University lab
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Feeding and food limitation in copepod nauplii, the neglected life stage
https://osprey.bco-dmo.org/project/546027
Feeding and food limitation in copepod nauplii, the neglected life stage
<p>This project will investigate feeding by copepod nauplius larvae, the most abundant metazoans in the sea. It will answer three questions: 1) How does food selection by adults and nauplii differ when they are fed multiple prey species in the laboratory? 2) How does food selection by adults and nauplii differ when they are feeding on natural prey assemblages? and, 3) How do growth, development, and survival differ between copepodites and nauplii when their growth is food limited? Comparative experiments and field-based measurements will contrast the food consumed, and the effects of food limitation, between nauplii and later life stages. This contrast will include attributes of food such as size, taxon, and motility, and will include experiments with cultured prey offered singly or in a mixture, and natural prey, and apply genetic techniques to determine prey consumption by a predatory copepod. Copepods will be collected from the San Francisco Estuary, with four species selected for experiments to span taxonomic groups, sizes, salinity ranges, and general feeding behavior. A variety of techniques will be applied to account for the inevitable biases and limitations of each; all but one have previously been applied in our laboratories. These will include laboratory feeding experiments using cultured prey individually and in mixtures, and experiments using natural prey. Consumption of prey in experimental bottles will be measured as chlorophyll concentration and through particle counts by microscopy and flow cytometry. Radioactively labeled prey will be used in short incubations to determine feeding on particular prey types. Samples from the field will be examined for gut fluorescence. Separate experiments will determine how nauplii and copepodites survive and grow at different concentrations of food. Investigations of feeding by a predatory copepod (Tortanus dextrilobatus) will use molecular techniques to identify mitochondrial and nuclear DNA from diverse suspected prey species. Specific primers will be developed for common zooplankton species consumed by T. dextrilobatus in the laboratory. General primers and screening protocols developed here will be useful for identifying food web interactions in other estuarine communities.</p>
<p>Copepod nauplii are important both in their diverse trophic roles in ocean foodwebs and in the population dynamics of copepods. Nauplii have a completely different feeding apparatus from later stages, and the first feeding stage can be very sensitive to starvation, making these life stages critical to population dynamics. Yet extant copepod population models treat nauplii as miniature adults. This work will provide valuable input to the growing efforts at modeling ocean ecosystems. </p>
<p><a href="http://dmoserv3.bco-dmo.org/data_docs/Food_limits_copepod_nauplii/Project_further_details_Kimmerer_copepod_naup_feeding.pdf" target="_blank">Further details from final report (pdf)</a></p>
food limitation in copepod nauplii
largerWorkCitation
project
eng; USA
biota
oceans
San Francisco State University lab
2015-01-15
San Francisco Estuary
0
BCO-DMO catalogue of parameters from Experimental results describing copepod gut evacuation time from species collected in the San Francisco estuary during 2013
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/546516.rdf
Name: replicate
Units: unitless
Description: <p>replicate number</p>
http://lod.bco-dmo.org/id/dataset-parameter/546517.rdf
Name: time
Units: minutes
Description: <p>time elapsed</p>
http://lod.bco-dmo.org/id/dataset-parameter/546518.rdf
Name: fluorescence
Units: relative fluorescence units??
Description: <p>fluorescence value</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
745
https://datadocs.bco-dmo.org/file/ZZZgKkNT5rYZEX/3a_Pmar_evac.csv
3a_Pmar_evac.csv
Primary data file for dataset ID 546506
download
https://osprey.bco-dmo.org/dataset/546506/data/download
download
onLine
dataset
<p>Copepods may have elevated feeding rates after being starved for as little as 6 h before they are provided with food (Tiselius 1998). Groups of 10 adult female <em>P. marinus</em> were sorted from laboratory cultures and pipetted into 500 mL Nalgene bottles containing GF/F filtered seawater, maintained at 19°C in a constant temperature room. Copepods were sampled at 10 min intervals up to 180 min, and their extracts were measured on the microplate reader as described in Vogt (2013). Most of the gut pigment was depurated within the first 30 min of incubation in filtered water, and no further depuration occurred after 60 min. Three hour starvation periods were chosen to account for potential differences in individual variability in depuration rate.</p>
Specified by the Principal Investigator(s)
<p>Raw data was plotted to determine gut fluorescence in raw units, subtracting background fluorescence from copepods with empty guts (background).</p>
<p><strong>BCO-DMO Processing:</strong><br />
- added conventional header with dataset name, PI name, version date, reference information<br />
- renamed parameters to BCO-DMO standard</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Fluorometer
Fluorometer
PI Supplied Instrument Name: Fluorometer PI Supplied Instrument Description:Turner 10AU Instrument Name: Fluorometer Instrument Short Name:Fluorometer Instrument Description: A fluorometer or fluorimeter is a device used to measure parameters of fluorescence: its intensity and wavelength distribution of emission spectrum after excitation by a certain spectrum of light. The instrument is designed to measure the amount of stimulated electromagnetic radiation produced by pulses of electromagnetic radiation emitted into a water sample or in situ. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/113/
PI Supplied Instrument Name: PI Supplied Instrument Description:Tecan Infinite F200 or Biotek Synergy 2 microplate reader was used for each analysis. Each microplate reader contained a 430/20 EX, 680/20 EMfilter pair for Chl a. Instrument Name: plate reader Instrument Short Name: Instrument Description: Plate readers (also known as microplate readers) are laboratory instruments designed to detect biological, chemical or physical events of samples in microtiter plates. They are widely used in research, drug discovery, bioassay validation, quality control and manufacturing processes in the pharmaceutical and biotechnological industry and academic organizations. Sample reactions can be assayed in 6-1536 well format microtiter plates. The most common microplate format used in academic research laboratories or clinical diagnostic laboratories is 96-well (8 by 12 matrix) with a typical reaction volume between 100 and 200 uL per well. Higher density microplates (384- or 1536-well microplates) are typically used for screening applications, when throughput (number of samples per day processed) and assay cost per sample become critical parameters, with a typical assay volume between 5 and 50 µL per well. Common detection modes for microplate assays are absorbance, fluorescence intensity, luminescence, time-resolved fluorescence, and fluorescence polarization. From: http://en.wikipedia.org/wiki/Plate_reader, 2014-09-0-23.
Spectrophotometer
Spectrophotometer
PI Supplied Instrument Name: Spectrophotometer PI Supplied Instrument Description:Agilent 8453 spectrophotometer (Agilent Technologies) Instrument Name: Spectrophotometer Instrument Short Name:Spectrophotometer Instrument Description: An instrument used to measure the relative absorption of electromagnetic radiation of different wavelengths in the near infra-red, visible and ultraviolet wavebands by samples. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB20/
Deployment: Kimmerer_2013
Kimmerer_2013
SFSU RTC
laboratory
Kimmerer_2013
William Kimmerer
San Francisco State University
SFSU RTC
laboratory