http://lod.bco-dmo.org/id/dataset/547774
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2015-02-03
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Results from experiment examining 15N-labeled contaminants in commercial 15N2 gas: Particulate Nitrogen - The delta 15N of Dunaliella tertiolecta organic matter after growth in media equilibrated with 15N2 gas
2015-02-03
publication
2015-02-03
revision
BCO-DMO Linked Data URI
2015-02-03
creation
http://lod.bco-dmo.org/id/dataset/547774
Julie Granger
University of Connecticut
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Granger, J. (2015) Results from experiment examining 15N-labeled contaminants in commercial 15N2 gas: Particulate Nitrogen - The delta 15N of Dunaliella tertiolecta organic matter after growth in media equilibrated with 15N2 gas. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 03 Feb 2015) Version Date 2015-02-03 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/547774 [access date]
Particulate Nitrogen - The delta 15N of Dunaliella tertiolecta organic matter after growth in media equilibrated with 15N2 gas. Dataset Description: <p>The delta 15N of <em>Dunaliella tertiolecta</em> organic matter after growth in media equilibrated with 15N2 gas.</p>
<p>Refer to the following publication for more information:<br />
Dabundo, R., Lehmann, M.F., Treibergs, L., Tobias, C.R., Altabet, M.A., Moisander, P.H., and Granger, J. 2014. The Contamination of Commercial 15N2 Gas Stocks with 15N–Labeled Nitrate and Ammonium and Consequences for Nitrogen Fixation Measurements. PLoS ONE, 9(10): e110335. doi:<a href="http://dx.doi.org/10.1371/journal.pone.0110335" target="_blank">10.1371/journal.pone.0110335</a></p>
<p>See related datasets:<br />
<a href="http://www.bco-dmo.org/dataset/542153">delta 15N NO3</a><br />
<a href="http://www.bco-dmo.org/dataset/542884">delta 15N NH4</a><br />
<a href="http://www.bco-dmo.org/dataset/546779">direct N2O</a><br />
<a href="http://www.bco-dmo.org/dataset/549112">N2</a></p> Methods and Sampling: <p>Data was acquired from an isotope ratio mass spectrometer using Isodat 3.0 software.</p>
<p><strong>Particulate Nitrogen calculations:</strong><br />
The equation describing the linear regression of the standards' observed delta 15N PN and Assigned delta 15N (obs = x and assigned = y) was used to calculate delta 15N PN (y) of the samples from the uncorrected delta 15N (x).</p>
<p><strong>Refer to the following publication for more information:</strong><br />
Dabundo, R., Lehmann, M.F., Treibergs, L., Tobias, C.R., Altabet, M.A., Moisander, P.H., and Granger, J. 2014. The Contamination of Commercial 15N2 Gas Stocks with 15N–Labeled Nitrate and Ammonium and Consequences for Nitrogen Fixation Measurements. PLoS ONE, 9(10): e110335. doi:<a href="http://dx.doi.org/10.1371/journal.pone.0110335" target="_blank">10.1371/journal.pone.0110335</a></p>
<p><strong>Summary of methods from Dabundo et al. 2014:</strong><br />
<em>Reagents:</em><br />
Four lecture bottles of 98+ at% 15N-labeled N2 gas were purchased from Sigma-Aldrich, three from lot # SZ1670V, and one from lot # MBBB0968V. Two 1L lecture bottles of 98+ at% 15N2 were purchased from Cambridge Isotopes from lot #’s I1-11785A and I-16727. One 1L lecture bottle of 98+ at% 15N2 was purchased from Campro Scientific from lot # EB1169V. Ammonium and nitrate solutions were prepared with salts or with solutions obtained from different distributors: sodium nitrate (NaNO3), potassium nitrate (KNO3), and ammonium chloride (NH4Cl) from Fisher Scientific; analytical-grade potassium nitrate from Fluka Analytical and a gravimetric solution of ammonium chloride from SPEX CertiPrep.</p>
<p><em>Dunaliella tertiolecta cultures:</em><br />
The marine green alga <em>Dunaliella tertiolecta</em> was cultured in growth media equilibrated with 15N2 gas in order to ascertain the susceptibility of 15N-labeled gas contaminants to assimilation by non-N2-fixing organisms. Culture medium was prepared from filtered Long Island Sound sea water supplemented with 50 umol/L NaNO3, 36.3 umol/L NaH2PO4*H2O, and 107 umol/L Na2SiO3*9H2O, as well as f/2 trace metals and f/2 vitamins, added from filter sterilized stock solutions. Medium (200 mL) was dispensed in 250 mL stoppered glass bottles. Experimental treatment bottles were equilibrated overnight with 0.2 mL 15N2 gas from either a Cambridge Isotopes or Sigma-Aldrich lecture bottle. Following inoculation, cultures were left loosely capped and placed on a windowsill with exposure to natural light. Nitrate concentrations were monitored daily. Upon the complete depletion of nitrate, 8 days after inoculation, the cultures were harvested on pre-combusted GF/F filters. Filters were dried at 60 degrees C for 18 hours pending N isotopic analysis of the particulate nitrogen.</p>
<p><em>Particulate nitrogen isotope ratio analyses:</em><br />
The d15N of particulate nitrogen (PN) was analyzed using a Costech Instruments elemental combustion system (model 4010) coupled to a Thermo Scientific Delta V Advantage IRMS. Analyses were standardized using L-glutamic acid reference materials, USGS-40, and USGS-41.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1233897 Award URL: http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1233897
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1130495 Award URL: http://www.nsf.gov/awardsearch/showAward?AWD_ID=1130495
Funding provided by Swiss National Science Foundation (SNSF) Award Number: R Equip 121258
completed
Julie Granger
University of Connecticut
860-405-9094
Department of Marine Sciences 1080 Shenecossett Road
Groton
CT
06340
USA
julie.granger@uconn.edu
pointOfContact
asNeeded
Dataset Version: 03 Feb 2015
Unknown
lecture_bottle
lot_number
solution
headspace
N2_injection
sample_ID
initial_NO3
final_NO3
uncorr_d15N
d15N_PN
assigned_d15N
Thermo Scientific Delta V Advantage IRMS
Costech Instruments 4010
theme
None, User defined
No BCO-DMO term
sample identification
featureType
BCO-DMO Standard Parameters
Isotope-ratio Mass Spectrometer
CHN Elemental Analyzer
instrument
BCO-DMO Standard Instruments
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
The Ocean Nitrogen Imbalance Paradox: Environmental Controls on the Denitrification Isotope Effect
https://www.bco-dmo.org/project/528935
The Ocean Nitrogen Imbalance Paradox: Environmental Controls on the Denitrification Isotope Effect
<p><em>Description from NSF award abstract:</em><br />
This study will test the sensitivity of the amplitude of the denitrification isotope effect to culture conditions pertinent to the ocean environment. The isotope effect amplitude will be explored with respect to electron donor, trace oxygenation, and temperature, in both batch and continuous culture experiments of denitrifiers. The proposed work will also involve measurements of the enzymatic isotope effect of the respiratory nitrate reductase of denitrifiers, measurements of its enzymatic activity among cultures, and examination of cellular nitrate transport kinetics of denitrifying strains. The experiments are designed to reveal the physiological basis of the modulation of the isotope effect amplitude, which will further resolve this manifestation in the environment.</p>
<p>In regards to the broader significance and importance of this study, these new experimental data will provide a basis for integration of nitrogen isotope dynamics in ocean models to test how key environmental parameters can affect the global ocean distribution of nitrogen isotopes.</p>
15N2 Contamination
largerWorkCitation
project
eng; USA
oceans
2015-02-03
0
BCO-DMO catalogue of parameters from Results from experiment examining 15N-labeled contaminants in commercial 15N2 gas: Particulate Nitrogen - The delta 15N of Dunaliella tertiolecta organic matter after growth in media equilibrated with 15N2 gas
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/547823.rdf
Name: lecture_bottle
Units: dimensionless
Description: Identifier of the lecture bottle of 15N-labeled N2 gas.
http://lod.bco-dmo.org/id/dataset-parameter/547824.rdf
Name: lot_number
Units: dimensionless
Description: Lot number of the 15N-labeled N2 gas; or 'Control' or 'Standard' for controls and standards respectively.
http://lod.bco-dmo.org/id/dataset-parameter/547825.rdf
Name: solution
Units: milliliters (mL)
Description: Solution volume.
http://lod.bco-dmo.org/id/dataset-parameter/547826.rdf
Name: headspace
Units: milliliters (mL)
Description: Headspace volume.
http://lod.bco-dmo.org/id/dataset-parameter/547827.rdf
Name: N2_injection
Units: milliliters (mL)
Description: Quantity of 15N-labeled N2 gas supplemented in the headspace.
http://lod.bco-dmo.org/id/dataset-parameter/547828.rdf
Name: sample_ID
Units: dimensionless
Description: Sample identification number.
http://lod.bco-dmo.org/id/dataset-parameter/547829.rdf
Name: initial_NO3
Units: micromolar (uM)
Description: Initial concentration of nitrate (NO3).
http://lod.bco-dmo.org/id/dataset-parameter/547830.rdf
Name: final_NO3
Units: micromolar (uM)
Description: Final concentration of nitrate (NO3).
http://lod.bco-dmo.org/id/dataset-parameter/547831.rdf
Name: uncorr_d15N
Units: per mille (‰)
Description: Uncorrected delta 15N.
http://lod.bco-dmo.org/id/dataset-parameter/547832.rdf
Name: d15N_PN
Units: per mille (‰)
Description: delta 15N of particulate Nitrogen (PN).
http://lod.bco-dmo.org/id/dataset-parameter/547833.rdf
Name: assigned_d15N
Units: per mille (‰)
Description: Assigned delta 15N.
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2192
https://datadocs.bco-dmo.org/file/5AAMvmocxwlD5Z/particulate_N.csv
particulate_N.csv
Primary data file for dataset ID 547774
download
https://www.bco-dmo.org/dataset/547774/data/download
download
onLine
dataset
<p>Data was acquired from an isotope ratio mass spectrometer using Isodat 3.0 software.</p>
<p><strong>Particulate Nitrogen calculations:</strong><br />
The equation describing the linear regression of the standards' observed delta 15N PN and Assigned delta 15N (obs = x and assigned = y) was used to calculate delta 15N PN (y) of the samples from the uncorrected delta 15N (x).</p>
<p><strong>Refer to the following publication for more information:</strong><br />
Dabundo, R., Lehmann, M.F., Treibergs, L., Tobias, C.R., Altabet, M.A., Moisander, P.H., and Granger, J. 2014. The Contamination of Commercial 15N2 Gas Stocks with 15N–Labeled Nitrate and Ammonium and Consequences for Nitrogen Fixation Measurements. PLoS ONE, 9(10): e110335. doi:<a href="http://dx.doi.org/10.1371/journal.pone.0110335" target="_blank">10.1371/journal.pone.0110335</a></p>
<p><strong>Summary of methods from Dabundo et al. 2014:</strong><br />
<em>Reagents:</em><br />
Four lecture bottles of 98+ at% 15N-labeled N2 gas were purchased from Sigma-Aldrich, three from lot # SZ1670V, and one from lot # MBBB0968V. Two 1L lecture bottles of 98+ at% 15N2 were purchased from Cambridge Isotopes from lot #’s I1-11785A and I-16727. One 1L lecture bottle of 98+ at% 15N2 was purchased from Campro Scientific from lot # EB1169V. Ammonium and nitrate solutions were prepared with salts or with solutions obtained from different distributors: sodium nitrate (NaNO3), potassium nitrate (KNO3), and ammonium chloride (NH4Cl) from Fisher Scientific; analytical-grade potassium nitrate from Fluka Analytical and a gravimetric solution of ammonium chloride from SPEX CertiPrep.</p>
<p><em>Dunaliella tertiolecta cultures:</em><br />
The marine green alga <em>Dunaliella tertiolecta</em> was cultured in growth media equilibrated with 15N2 gas in order to ascertain the susceptibility of 15N-labeled gas contaminants to assimilation by non-N2-fixing organisms. Culture medium was prepared from filtered Long Island Sound sea water supplemented with 50 umol/L NaNO3, 36.3 umol/L NaH2PO4*H2O, and 107 umol/L Na2SiO3*9H2O, as well as f/2 trace metals and f/2 vitamins, added from filter sterilized stock solutions. Medium (200 mL) was dispensed in 250 mL stoppered glass bottles. Experimental treatment bottles were equilibrated overnight with 0.2 mL 15N2 gas from either a Cambridge Isotopes or Sigma-Aldrich lecture bottle. Following inoculation, cultures were left loosely capped and placed on a windowsill with exposure to natural light. Nitrate concentrations were monitored daily. Upon the complete depletion of nitrate, 8 days after inoculation, the cultures were harvested on pre-combusted GF/F filters. Filters were dried at 60 degrees C for 18 hours pending N isotopic analysis of the particulate nitrogen.</p>
<p><em>Particulate nitrogen isotope ratio analyses:</em><br />
The d15N of particulate nitrogen (PN) was analyzed using a Costech Instruments elemental combustion system (model 4010) coupled to a Thermo Scientific Delta V Advantage IRMS. Analyses were standardized using L-glutamic acid reference materials, USGS-40, and USGS-41.</p>
Specified by the Principal Investigator(s)
<p>Samples with the same ID are replicated measurements.</p>
<p>BCO-DMO Edits:<br />
- Modified parameter names to conform with BCO-DMO naming conventions;<br />
- Denoted 'Control' and 'Standard' in the lot_number column;<br />
- Replaced spaces with underscores.<br />
- Replaced blanks (missing values) with 'nd' to indicate 'no data'.</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Thermo Scientific Delta V Advantage IRMS
Thermo Scientific Delta V Advantage IRMS
PI Supplied Instrument Name: Thermo Scientific Delta V Advantage IRMS PI Supplied Instrument Description:The delta 15N of particulate nitrogen (PN) was analyzed using a Costech Instruments elemental combustion system (model 4010) coupled to a Thermo Scientific Delta V Advantage IRMS. Instrument Name: Isotope-ratio Mass Spectrometer Instrument Short Name:IR Mass Spec; IRMS Instrument Description: The Isotope-ratio Mass Spectrometer is a particular type of mass spectrometer used to measure the relative abundance of isotopes in a given sample (e.g. VG Prism II Isotope Ratio Mass-Spectrometer). Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB16/
Costech Instruments 4010
Costech Instruments 4010
PI Supplied Instrument Name: Costech Instruments 4010 PI Supplied Instrument Description:The delta 15N of particulate nitrogen (PN) was analyzed using a Costech Instruments elemental combustion system (model 4010) coupled to a Thermo Scientific Delta V Advantage IRMS. Instrument Name: CHN Elemental Analyzer Instrument Short Name:CHN_EA Instrument Description: A CHN Elemental Analyzer is used for the determination of carbon, hydrogen, and nitrogen content in organic and other types of materials, including solids, liquids, volatile, and viscous samples.