http://lod.bco-dmo.org/id/dataset/549112
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2015-02-04
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Results from experiment examining 15N-labeled contaminants in commercial 15N2 gas: The measured quantity of 15N2 added to nitrate and ammonium solutions equilibrated with 15N2 gas
2015-02-04
publication
2015-02-04
revision
BCO-DMO Linked Data URI
2015-02-04
creation
http://lod.bco-dmo.org/id/dataset/549112
Julie Granger
University of Connecticut
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Granger, J. (2015) Results from experiment examining 15N-labeled contaminants in commercial 15N2 gas: The measured quantity of 15N2 added to nitrate and ammonium solutions equilibrated with 15N2 gas. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 04 Feb 2015) Version Date 2015-02-04 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/549112 [access date]
The measured quantity of 15N2 added to nitrate and ammonium solutions equilibrated with 15N2 gas. Dataset Description: <p>The measured quantity of 15N2 added to nitrate and ammonium solutions equilibrated with 15N2 gas.</p>
<p>Refer to the following publication for more information:<br />
Dabundo, R., Lehmann, M.F., Treibergs, L., Tobias, C.R., Altabet, M.A., Moisander, P.H., and Granger, J. 2014. The Contamination of Commercial 15N2 Gas Stocks with 15N–Labeled Nitrate and Ammonium and Consequences for Nitrogen Fixation Measurements. PLoS ONE, 9(10): e110335. doi:<a href="http://dx.doi.org/10.1371/journal.pone.0110335" target="_blank">10.1371/journal.pone.0110335</a></p>
<p>See related datasets:<br />
<a href="http://www.bco-dmo.org/dataset/542153">delta 15N NO3</a><br />
<a href="http://www.bco-dmo.org/dataset/542884">delta 15N NH4</a><br />
<a href="http://www.bco-dmo.org/dataset/546779">direct N2O</a><br />
<a href="http://www.bco-dmo.org/dataset/547774">particulate N</a></p> Methods and Sampling: <p>Data was acquired from an isotope ratio mass spectrometer using Isodat 3.0 software.</p>
<p><strong>Dinitrogen (N2) calculations:</strong><br />
Moles of 15N2 added were calculated from 28, 29, and 30 areas compared to the atmosphere.</p>
<p>Calculation:<br />
atm = atmosphere<br />
variables labeled “A” correspond to measured area values</p>
<p>Constants:<br />
15Natm mole fraction = 0.003663<br />
14Natm mole fraction = 0.996337<br />
Therefore the fraction of&nbsp; 30N2atm = 1.34176E-05; 29N2atm = 0.007299165; 28N2atm = 0.992687418<br />
30N2atm/ 28N2atm = 1.35164E-05 = 30/28natural_abundance<br />
28N2 total =&nbsp; 28N2 total in incubation was calculated from the ideal gas law at 25 degrees C and Henry’s Law. Found to be 654 umol in incubations with 40 mL solution and 20 mL headspace, and 690 umol in incubations with 100 mL solution and 20 mL headspace.</p>
<p>The following equations were applied to air standards to correct for the blank 30/28:<br />
30area from binomial = 28A * 30/28nat_abundance<br />
30/28area ratio blank = (30A/28A) – 30area_from_binomial/28A<br />
30/28area ratio blank is graphed (y axis) vs. 28A (x axis) to account for variation relating to the injection volume.<br />
The slope (mratio blank) and intercept (iratio blank) of the corresponding linear regression are found.</p>
<p>The blank is then removed from the measured 30A/28A ratio of samples:<br />
30A/28A real = 30A/28A – (mratio_blank * 28A + iratio_blank)</p>
<p>Added 30N2 is calculated:<br />
30N2total = 30A/28A real * 28N2total<br />
30N2added = 30N2total – (28N2total * 30/28natural_abundance)</p>
<p>Total 15N added = 2 *&nbsp; 30N2added + 29N2added. However, 29N2added was negligible, as expected from the 98+ atom % 15N2 gas. Therefore, the reported 15N2 gas added is equivalent to added 30N2.</p>
<p><strong>Summary of methods from Dabundo et al. 2014:</strong><br />
<em>Reagents:</em><br />
Four lecture bottles of 98+ at% 15N-labeled N2 gas were purchased from Sigma-Aldrich, three from lot # SZ1670V, and one from lot # MBBB0968V. Two 1L lecture bottles of 98+ at% 15N2 were purchased from Cambridge Isotopes from lot #’s I1-11785A and I-16727. One 1L lecture bottle of 98+ at% 15N2 was purchased from Campro Scientific from lot # EB1169V. Ammonium and nitrate solutions were prepared with salts or with solutions obtained from different distributors: sodium nitrate (NaNO3), potassium nitrate (KNO3), and ammonium chloride (NH4Cl) from Fisher Scientific; analytical-grade potassium nitrate from Fluka Analytical and a gravimetric solution of ammonium chloride from SPEX CertiPrep.</p>
<p><em>Preparation of nitrate &amp; ammonium solutions:</em><br />
Aqueous solutions of natural abundance (unlabeled) ammonium and nitrate salts were equilibrated overnight with an air headspace supplemented with an injection of 15N2 gas (to determine whether the 15N2 gas stocks contained 15N-labeled ammonia (NH3) or nitrate and/or nitrite (NOx) contaminants). After equilibration, the 15N/14N ratio of ammonium and the 15N/14N and 18O/16O ratios of nitrate/nitrite in solution were measured, as well as the 15N/14N ratio of N2 gas in the headspace. The isotope ratios of nitrate and ammonium were compared to those in control solutions, which were not supplemented with 15N2 gas. Experiments with the Campro Scientific 15N2 stock were verified for 15N-nitrate/nitrite contaminants only (and not for 15N-ammonium).</p>
<p>Initial experiments consisted of 40 mL or 100 mL solutions of 10, 50, 100, 200, or 300 umol/L nitrate and 5 umol/L ammonium chloride in 60 mL or 120 mL serum vials that were sealed with stoppers. The 20 mL of air headspace in each of the treatment vials was supplemented with 0.1 mL of 15N2 gas from respective bottles from each of the three suppliers. The solutions were equilibrated overnight on a shaker, after which the 15N/14N and 18O/16O isotope ratios of nitrate were analyzed. The 15N/14N isotope ratio of ammonium was also analyzed in experimental solutions treated with the Sigma-Aldrich and Cambridge Isotopes stocks.</p>
<p>Additional experiments were carried out in which 2 mL 15N2 gas was equilibrated overnight in 20 mL serum vials containing 10 mL solutions of 10 umol/L sodium nitrate, after which the 15N/14N and 18O/16O ratios of nitrate were measured. Similarly, 10 mL solutions of 5 umol/L ammonium chloride were dispensed in 20 mL serum vials and equilibrated overnight with 2 mL 15N2 gas, after which the 15N/14N isotope ratios of ammonium were analyzed.</p>
<p><em>Nitrate and ammonium concentrations:</em><br />
Nitrate concentrations in the experimental solutions were verified via reduction to nitric oxide in hot vanadium (III) solution followed by detection with a chemiluminescence NOx analyzer (model T200 Teledyne Advanced Pollution Instrumentation). Ammonium concentrations were measured by derivatization with orthophthaldialdehyde (OPA) and fluorometric detection on an AJN Scientific f-2500 Fluorescence Spectrophotometer.</p>
<p><em>Nitrate N and O isotope ratio analyses:</em><br />
Nitrate/nitrite nitrogen (15N/14N) and oxygen (18O/16O) isotope ratios were measured using the denitrifier method. Nitrate (and nitrite) in experimental samples was converted stoichiometrically to nitrous oxide (N2O) by a denitrifying bacterial strain (Pseudomonas chlororaphis f. sp. aureofaciens, ATCC 13985) that lacks nitrous oxide reductase. The N and O isotopic composition of N2O was then measured on a Delta V Advantage Isotope Ratio Mass Spectrometer (IRMS) interfaced with a modified Gas Bench II gas chromatograph (Thermo Fisher) purge and trap system. The isotope ratio measurements are reported in per mille (o/oo) units.</p>
<p>The 15N/14N reference is N2 in air, and the 18O/16O reference is Vienna Standard Mean Ocean water (V-SMOW). Individual analyses on the GC-IRMS were referenced to injections of N2O from a pure N2O gas cylinder, and then standardized through comparison to the international nitrate standards USGS-34, USGS-32, and IAEA-NO-3, using standard bracketing techniques. Nitrate samples from experiments with Campro Scientific 15N2 were standardized with USGS-32 and IAEA-NO-3, and an additional internal lab nitrate standard (UBN-1).</p>
<p><em>Headspace N2 isotope ratio analyses:</em><br />
To measure the d15N of N2 gas in the headspace of experimental samples, 75 uL of headspace was injected into 12 mL Exetainer vials previously flushed with helium, then analyzed on a Gas Bench II GC-IRMS (Delta V Advantage Plus) operated in continuous flow mode. N2 and (O2+ Ar) were separated on a gas chromatography column. The analyses were standardized with parallel analyses of ambient N2 gas in air. These direct N2 gas measurements were carried out for experiments conducted using two of three lecture bottles from Sigma-Aldrich lot, and for experiments conducted using the lecture bottle from Cambridge Isotopes. The 15N2 concentration in the headspace of other experiments was estimated from the tracer injection volume rather than from direct measurements.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1233897 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1233897
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1130495 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1130495
Funding provided by Swiss National Science Foundation (SNSF) Award Number: R Equip 121258
completed
Julie Granger
University of Connecticut
860-405-9094
Department of Marine Sciences 1080 Shenecossett Road
Groton
CT
06340
USA
julie.granger@uconn.edu
pointOfContact
asNeeded
Dataset Version: 04 Feb 2015
Unknown
lecture_bottle
lot_number
sample_ID
Area_28_Vxs
Area_29_Vxs
Area_30_Vxs
added_15N2
V_injection_air_standard
T200 Teledyne Advanced Pollution Instrumentation
Gas Chromatograph
Delta V Advantage Isotope Ratio Mass Spectrometer (IRMS)
AJN Scientific f-2500 Fluorescence Spectrophotometer
theme
None, User defined
No BCO-DMO term
sample identification
featureType
BCO-DMO Standard Parameters
Chemiluminescence NOx Analyzer
Gas Chromatograph
Isotope-ratio Mass Spectrometer
Spectrophotometer
instrument
BCO-DMO Standard Instruments
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
The Ocean Nitrogen Imbalance Paradox: Environmental Controls on the Denitrification Isotope Effect
https://osprey.bco-dmo.org/project/528935
The Ocean Nitrogen Imbalance Paradox: Environmental Controls on the Denitrification Isotope Effect
<p><em>Description from NSF award abstract:</em><br />
This study will test the sensitivity of the amplitude of the denitrification isotope effect to culture conditions pertinent to the ocean environment. The isotope effect amplitude will be explored with respect to electron donor, trace oxygenation, and temperature, in both batch and continuous culture experiments of denitrifiers. The proposed work will also involve measurements of the enzymatic isotope effect of the respiratory nitrate reductase of denitrifiers, measurements of its enzymatic activity among cultures, and examination of cellular nitrate transport kinetics of denitrifying strains. The experiments are designed to reveal the physiological basis of the modulation of the isotope effect amplitude, which will further resolve this manifestation in the environment.</p>
<p>In regards to the broader significance and importance of this study, these new experimental data will provide a basis for integration of nitrogen isotope dynamics in ocean models to test how key environmental parameters can affect the global ocean distribution of nitrogen isotopes.</p>
15N2 Contamination
largerWorkCitation
project
eng; USA
oceans
2015-02-04
0
BCO-DMO catalogue of parameters from Results from experiment examining 15N-labeled contaminants in commercial 15N2 gas: The measured quantity of 15N2 added to nitrate and ammonium solutions equilibrated with 15N2 gas
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/549130.rdf
Name: lecture_bottle
Units: dimensionless
Description: <p>Identifier of the lecture bottle of 15N-labeled N2 gas.</p>
http://lod.bco-dmo.org/id/dataset-parameter/549131.rdf
Name: lot_number
Units: dimensionless
Description: <p>Lot number of the 15N-labeled N2 gas; or 'Control' or 'Standard' for controls and standards respectively.</p>
http://lod.bco-dmo.org/id/dataset-parameter/549132.rdf
Name: sample_ID
Units: dimensionless
Description: <p>Sample identification number.</p>
http://lod.bco-dmo.org/id/dataset-parameter/549133.rdf
Name: Area_28_Vxs
Units: volt seconds
Description: <p>28 Area (V *s) - Volt second measurements of mass 28 nitrogen gas output by the mass spectrometer.</p>
http://lod.bco-dmo.org/id/dataset-parameter/549134.rdf
Name: Area_29_Vxs
Units: volt seconds
Description: <p>29 Area (V *s) - Volt second measurements of mass 29 nitrogen gas output by the mass spectrometer.</p>
http://lod.bco-dmo.org/id/dataset-parameter/549135.rdf
Name: Area_30_Vxs
Units: volt seconds
Description: <p>30 Area (V *s) - Volt second measurements of mass 30 nitrogen gas output by the mass spectrometer.</p>
http://lod.bco-dmo.org/id/dataset-parameter/549136.rdf
Name: added_15N2
Units: moles
Description: <p>Amount of added 15N2.</p>
http://lod.bco-dmo.org/id/dataset-parameter/549137.rdf
Name: V_injection_air_standard
Units: microliters (uL)
Description: <p>V injection (in microliters) for the air standards.</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
3151
https://datadocs.bco-dmo.org/file/7DDMxoxFZ4zDrM/N2.csv
N2.csv
Primary data file for dataset ID 549112
download
https://osprey.bco-dmo.org/dataset/549112/data/download
download
onLine
dataset
<p>Data was acquired from an isotope ratio mass spectrometer using Isodat 3.0 software.</p>
<p><strong>Dinitrogen (N2) calculations:</strong><br />
Moles of 15N2 added were calculated from 28, 29, and 30 areas compared to the atmosphere.</p>
<p>Calculation:<br />
atm = atmosphere<br />
variables labeled “A” correspond to measured area values</p>
<p>Constants:<br />
15Natm mole fraction = 0.003663<br />
14Natm mole fraction = 0.996337<br />
Therefore the fraction of&nbsp; 30N2atm = 1.34176E-05; 29N2atm = 0.007299165; 28N2atm = 0.992687418<br />
30N2atm/ 28N2atm = 1.35164E-05 = 30/28natural_abundance<br />
28N2 total =&nbsp; 28N2 total in incubation was calculated from the ideal gas law at 25 degrees C and Henry’s Law. Found to be 654 umol in incubations with 40 mL solution and 20 mL headspace, and 690 umol in incubations with 100 mL solution and 20 mL headspace.</p>
<p>The following equations were applied to air standards to correct for the blank 30/28:<br />
30area from binomial = 28A * 30/28nat_abundance<br />
30/28area ratio blank = (30A/28A) – 30area_from_binomial/28A<br />
30/28area ratio blank is graphed (y axis) vs. 28A (x axis) to account for variation relating to the injection volume.<br />
The slope (mratio blank) and intercept (iratio blank) of the corresponding linear regression are found.</p>
<p>The blank is then removed from the measured 30A/28A ratio of samples:<br />
30A/28A real = 30A/28A – (mratio_blank * 28A + iratio_blank)</p>
<p>Added 30N2 is calculated:<br />
30N2total = 30A/28A real * 28N2total<br />
30N2added = 30N2total – (28N2total * 30/28natural_abundance)</p>
<p>Total 15N added = 2 *&nbsp; 30N2added + 29N2added. However, 29N2added was negligible, as expected from the 98+ atom % 15N2 gas. Therefore, the reported 15N2 gas added is equivalent to added 30N2.</p>
<p><strong>Summary of methods from Dabundo et al. 2014:</strong><br />
<em>Reagents:</em><br />
Four lecture bottles of 98+ at% 15N-labeled N2 gas were purchased from Sigma-Aldrich, three from lot # SZ1670V, and one from lot # MBBB0968V. Two 1L lecture bottles of 98+ at% 15N2 were purchased from Cambridge Isotopes from lot #’s I1-11785A and I-16727. One 1L lecture bottle of 98+ at% 15N2 was purchased from Campro Scientific from lot # EB1169V. Ammonium and nitrate solutions were prepared with salts or with solutions obtained from different distributors: sodium nitrate (NaNO3), potassium nitrate (KNO3), and ammonium chloride (NH4Cl) from Fisher Scientific; analytical-grade potassium nitrate from Fluka Analytical and a gravimetric solution of ammonium chloride from SPEX CertiPrep.</p>
<p><em>Preparation of nitrate &amp; ammonium solutions:</em><br />
Aqueous solutions of natural abundance (unlabeled) ammonium and nitrate salts were equilibrated overnight with an air headspace supplemented with an injection of 15N2 gas (to determine whether the 15N2 gas stocks contained 15N-labeled ammonia (NH3) or nitrate and/or nitrite (NOx) contaminants). After equilibration, the 15N/14N ratio of ammonium and the 15N/14N and 18O/16O ratios of nitrate/nitrite in solution were measured, as well as the 15N/14N ratio of N2 gas in the headspace. The isotope ratios of nitrate and ammonium were compared to those in control solutions, which were not supplemented with 15N2 gas. Experiments with the Campro Scientific 15N2 stock were verified for 15N-nitrate/nitrite contaminants only (and not for 15N-ammonium).</p>
<p>Initial experiments consisted of 40 mL or 100 mL solutions of 10, 50, 100, 200, or 300 umol/L nitrate and 5 umol/L ammonium chloride in 60 mL or 120 mL serum vials that were sealed with stoppers. The 20 mL of air headspace in each of the treatment vials was supplemented with 0.1 mL of 15N2 gas from respective bottles from each of the three suppliers. The solutions were equilibrated overnight on a shaker, after which the 15N/14N and 18O/16O isotope ratios of nitrate were analyzed. The 15N/14N isotope ratio of ammonium was also analyzed in experimental solutions treated with the Sigma-Aldrich and Cambridge Isotopes stocks.</p>
<p>Additional experiments were carried out in which 2 mL 15N2 gas was equilibrated overnight in 20 mL serum vials containing 10 mL solutions of 10 umol/L sodium nitrate, after which the 15N/14N and 18O/16O ratios of nitrate were measured. Similarly, 10 mL solutions of 5 umol/L ammonium chloride were dispensed in 20 mL serum vials and equilibrated overnight with 2 mL 15N2 gas, after which the 15N/14N isotope ratios of ammonium were analyzed.</p>
<p><em>Nitrate and ammonium concentrations:</em><br />
Nitrate concentrations in the experimental solutions were verified via reduction to nitric oxide in hot vanadium (III) solution followed by detection with a chemiluminescence NOx analyzer (model T200 Teledyne Advanced Pollution Instrumentation). Ammonium concentrations were measured by derivatization with orthophthaldialdehyde (OPA) and fluorometric detection on an AJN Scientific f-2500 Fluorescence Spectrophotometer.</p>
<p><em>Nitrate N and O isotope ratio analyses:</em><br />
Nitrate/nitrite nitrogen (15N/14N) and oxygen (18O/16O) isotope ratios were measured using the denitrifier method. Nitrate (and nitrite) in experimental samples was converted stoichiometrically to nitrous oxide (N2O) by a denitrifying bacterial strain (Pseudomonas chlororaphis f. sp. aureofaciens, ATCC 13985) that lacks nitrous oxide reductase. The N and O isotopic composition of N2O was then measured on a Delta V Advantage Isotope Ratio Mass Spectrometer (IRMS) interfaced with a modified Gas Bench II gas chromatograph (Thermo Fisher) purge and trap system. The isotope ratio measurements are reported in per mille (o/oo) units.</p>
<p>The 15N/14N reference is N2 in air, and the 18O/16O reference is Vienna Standard Mean Ocean water (V-SMOW). Individual analyses on the GC-IRMS were referenced to injections of N2O from a pure N2O gas cylinder, and then standardized through comparison to the international nitrate standards USGS-34, USGS-32, and IAEA-NO-3, using standard bracketing techniques. Nitrate samples from experiments with Campro Scientific 15N2 were standardized with USGS-32 and IAEA-NO-3, and an additional internal lab nitrate standard (UBN-1).</p>
<p><em>Headspace N2 isotope ratio analyses:</em><br />
To measure the d15N of N2 gas in the headspace of experimental samples, 75 uL of headspace was injected into 12 mL Exetainer vials previously flushed with helium, then analyzed on a Gas Bench II GC-IRMS (Delta V Advantage Plus) operated in continuous flow mode. N2 and (O2+ Ar) were separated on a gas chromatography column. The analyses were standardized with parallel analyses of ambient N2 gas in air. These direct N2 gas measurements were carried out for experiments conducted using two of three lecture bottles from Sigma-Aldrich lot, and for experiments conducted using the lecture bottle from Cambridge Isotopes. The 15N2 concentration in the headspace of other experiments was estimated from the tracer injection volume rather than from direct measurements.</p>
Specified by the Principal Investigator(s)
<p>Samples with the same ID are replicated measurements.</p>
<p>BCO-DMO Edits:<br />
- Modified parameter names to conform with BCO-DMO naming conventions;<br />
- Replaced spaces with underscores.</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
T200 Teledyne Advanced Pollution Instrumentation
T200 Teledyne Advanced Pollution Instrumentation
PI Supplied Instrument Name: T200 Teledyne Advanced Pollution Instrumentation PI Supplied Instrument Description:Nitrate concentrations in the experimental solutions were verified via reduction to nitric oxide in hot vanadium (III) solution followed by detection with a chemiluminescence NOx analyzer (model T200 Teledyne Advanced Pollution Instrumentation). Instrument Name: Chemiluminescence NOx Analyzer Instrument Short Name: Instrument Description: The chemiluminescence method for gas analysis of oxides of nitrogen relies on the measurement of light produced by the gas-phase titration of nitric oxide and ozone. A chemiluminescence analyzer can measure the concentration of NO/NO2/NOX.
One example is the Teledyne Model T200: https://www.teledyne-api.com/products/nitrogen-compound-instruments/t200
Gas Chromatograph
Gas Chromatograph
PI Supplied Instrument Name: Gas Chromatograph PI Supplied Instrument Description:The N and O isotopic composition of N2O was then measured on a Delta V Advantage Isotope Ratio Mass Spectrometer (IRMS) interfaced with a modified Gas Bench II gas chromatograph (Thermo Fisher) purge and trap system.
To measure the d15N of N2 gas in the headspace of experimental samples, 75 uL of headspace was injected into 12 mL Exetainer vials previously flushed with helium, then analyzed on a Gas Bench II GC-IRMS (Delta V Advantage Plus) operated in continuous flow mode. Instrument Name: Gas Chromatograph Instrument Short Name:Gas Chromatograph Instrument Description: Instrument separating gases, volatile substances, or substances dissolved in a volatile solvent by transporting an inert gas through a column packed with a sorbent to a detector for assay. (from SeaDataNet, BODC) Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB02/
Delta V Advantage Isotope Ratio Mass Spectrometer (IRMS)
Delta V Advantage Isotope Ratio Mass Spectrometer (IRMS)
PI Supplied Instrument Name: Delta V Advantage Isotope Ratio Mass Spectrometer (IRMS) PI Supplied Instrument Description:The N and O isotopic composition of N2O was then measured on a Delta V Advantage Isotope Ratio Mass Spectrometer (IRMS) interfaced with a modified Gas Bench II gas chromatograph (Thermo Fisher) purge and trap system.
To measure the d15N of N2 gas in the headspace of experimental samples, 75 uL of headspace was injected into 12 mL Exetainer vials previously flushed with helium, then analyzed on a Gas Bench II GC-IRMS (Delta V Advantage Plus) operated in continuous flow mode. Instrument Name: Isotope-ratio Mass Spectrometer Instrument Short Name:IR Mass Spec; IRMS Instrument Description: The Isotope-ratio Mass Spectrometer is a particular type of mass spectrometer used to measure the relative abundance of isotopes in a given sample (e.g. VG Prism II Isotope Ratio Mass-Spectrometer). Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB16/
AJN Scientific f-2500 Fluorescence Spectrophotometer
AJN Scientific f-2500 Fluorescence Spectrophotometer
PI Supplied Instrument Name: AJN Scientific f-2500 Fluorescence Spectrophotometer PI Supplied Instrument Description:Ammonium concentrations were measured by derivatization with orthophthaldialdehyde (OPA) and fluorometric detection on an AJN Scientific f-2500 Fluorescence Spectrophotometer. Instrument Name: Spectrophotometer Instrument Short Name:Spectrophotometer Instrument Description: An instrument used to measure the relative absorption of electromagnetic radiation of different wavelengths in the near infra-red, visible and ultraviolet wavebands by samples. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB20/