http://lod.bco-dmo.org/id/dataset/549122
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2015-02-04
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Cellular elemental content of individual phytoplankton cells collected during US GEOTRACES North Atlantic Transect cruises in the Subtropical western and eastern North Atlantic Ocean during Oct and Nov, 2010 and Nov. 2011
2015-02-17
publication
2015-02-17
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2016-03-29
publication
https://doi.org/10.1575/1912/bco-dmo.641155
Benjamin Twining
Bigelow Laboratory for Ocean Sciences
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Twining, B. (2015) Cellular elemental content of individual phytoplankton cells collected during US GEOTRACES North Atlantic Transect cruises in the Subtropical western and eastern North Atlantic Ocean during Oct and Nov, 2010 and Nov. 2011. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 2015-02-17) Version Date 2015-02-17 [if applicable, indicate subset used]. doi:10.1575/1912/bco-dmo.641155 [access date]
Element quotas of individual phytoplankton cells Dataset Description: <p>Individual phytoplankton cells were collected on the GEOTRACES North Atlantic Transect cruises were analyzed for elemental content using SXRF (Synchrotron radiation X-Ray Fluorescence). Carbon was calculated from biovolume using the relationships of Menden-Deuer &amp; Lessard (2000). Trace metal concentrations are reported.</p>
<p><strong>Download zipped images:</strong><br />
<a href="http://data.bco-dmo.org/GEOTRACES/Twining/Chl_image.zip">Chlorophyll</a><br />
<a href="http://data.bco-dmo.org/GEOTRACES/Twining/Light_image.zip">Light</a><br />
<a href="http://data.bco-dmo.org/GEOTRACES/Twining/SXRF_map.zip">SXRF maps</a><br />
<a href="http://data.bco-dmo.org/GEOTRACES/Twining/SXRF_spectra.zip">SXRF spectra</a></p>
<p><strong>Related references:</strong></p>
<p>Menden-Deuer, S. and E. J. Lessard (2000). Carbon to volume relationships for dinoflagellates, diatoms, and other protist plankton. Limnology and Oceanography 45(3): 569-579.</p>
<p>* Twining, B. S., S. Rauschenberg, P. L. Morton, and S. Vogt. 2015. Metal contents of phytoplankton and labile particulate material in the North Atlantic Ocean. Progress in Oceanography 137: 261-283.)</p> Methods and Sampling: <p>SXRF samples were prepared from unfiltered water taken from GEOTRACES GO-Flo bottles at the shallowest depth and deep chlorophyll maximum. Cells were preserved with 0.25% trace-metal clean buffered glutaraldehyde and centrifuged onto C/formvar-coated Au TEM grids. Grids were briefly rinsed with a drop of ultrapure water and dried in a Class-100 cabinet. SXRF analysis was performed using the 2-ID-E beamline at the Advanced Photon source (Argonne National Laboratory) following the protocols of Twining et al. (2011).</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-0928289 Award URL: http://www.nsf.gov/awardsearch/showAward?AWD_ID=0928289&HistoricalAwards=false
completed
Benjamin Twining
Bigelow Laboratory for Ocean Sciences
207-315-2567
60 Bigelow Drive PO Box 380
East Boothbay
ME
04544-0380
USA
btwining@bigelow.org
pointOfContact
asNeeded
Dataset Version: 2015-02-17
Unknown
cruise_id
station_GEOTRC
lat
lon
cast_GEOTRC
event_GEOTRC
depth_GEOTRC_CTD
depth_GEOTRC_CTD_round
sample_GEOTRC
sample_bottle_GEOTRC
bottle_GEOTRC
project
grid_type
grid_num
SXRF_run
mda_id
cell_type
cell_vol
cell_C
cell_Si
cell_P
cell_S
cell_Mn
cell_Fe
cell_Co
cell_Ni
cell_Cu
cell_Zn
light_image_filename
chl_image_filename
SXRF_map_filename
SXRF_spectrum_filename
BTL_ISO_DateTime_UTC
sta_PI
theme
None, User defined
cruise id
station
latitude
longitude
cast
event
depth
sample identification
bottle
project
No BCO-DMO term
volume
biomass carbon
trace metal concentration
ISO_DateTime_UTC
station number
featureType
BCO-DMO Standard Parameters
GO-FLO Bottle
GeoFish Towed near-Surface Sampler
Microscope - Optical
X-ray fluorescence analyzer
instrument
BCO-DMO Standard Instruments
KN199-04
KN199-05
KN204-01
service
Deployment Activity
Subtropical northern Atlantic Ocean
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
U.S. GEOTRACES
http://www.geotraces.org/
U.S. GEOTRACES
GEOTRACES is a SCOR sponsored program; and funding for program infrastructure development is provided by the U.S. National Science Foundation.
GEOTRACES gained momentum following a special symposium, S02: Biogeochemical cycling of trace elements and isotopes in the ocean and applications to constrain contemporary marine processes (GEOSECS II), at a 2003 Goldschmidt meeting convened in Japan. The GEOSECS II acronym referred to the Geochemical Ocean Section Studies To determine full water column distributions of selected trace elements and isotopes, including their concentration, chemical speciation, and physical form, along a sufficient number of sections in each ocean basin to establish the principal relationships between these distributions and with more traditional hydrographic parameters;
* To evaluate the sources, sinks, and internal cycling of these species and thereby characterize more completely the physical, chemical and biological processes regulating their distributions, and the sensitivity of these processes to global change; and
* To understand the processes that control the concentrations of geochemical species used for proxies of the past environment, both in the water column and in the substrates that reflect the water column.
GEOTRACES will be global in scope, consisting of ocean sections complemented by regional process studies. Sections and process studies will combine fieldwork, laboratory experiments and modelling. Beyond realizing the scientific objectives identified above, a natural outcome of this work will be to build a community of marine scientists who understand the processes regulating trace element cycles sufficiently well to exploit this knowledge reliably in future interdisciplinary studies.
Expand "Projects" below for information about and data resulting from individual US GEOTRACES research projects.
U.S. GEOTRACES
largerWorkCitation
program
U.S. GEOTRACES North Atlantic Transect (GA03)
https://www.geotraces.org/
U.S. GEOTRACES North Atlantic Transect (GA03)
<p><em>Much of this text appeared in an article published in OCB News, October 2008, by the OCB Project Office.</em></p>
<p>The first U.S. GEOTRACES Atlantic Section will be specifically centered around a sampling cruise to be carried out in the North Atlantic in 2010. Ed Boyle (MIT) and Bill Jenkins (WHOI) organized a three-day planning workshop that was held September 22-24, 2008 at the Woods Hole Oceanographic Institution. The main goal of the workshop, sponsored by the National Science Foundation and the U.S. GEOTRACES Scientific Steering Committee, was to design the implementation plan for the first U.S. GEOTRACES Atlantic Section. The primary cruise design motivation was to improve knowledge of the sources, sinks and internal cycling of Trace Elements and their Isotopes (TEIs) by studying their distributions along a section in the North Atlantic (Figure 1). The North Atlantic has the full suite of processes that affect TEIs, including strong meridional advection, boundary scavenging and source effects, aeolian deposition, and the salty Mediterranean Outflow. The North Atlantic is particularly important as it lies at the "origin" of the global Meridional Overturning Circulation.</p>
<p>It is well understood that many trace metals play important roles in biogeochemical processes and the carbon cycle, yet very little is known about their large-scale distributions and the regional scale processes that affect them. Recent advances in sampling and analytical techniques, along with advances in our understanding of their roles in enzymatic and catalytic processes in the open ocean provide a natural opportunity to make substantial advances in our understanding of these important elements. Moreover, we are motivated by the prospect of global change and the need to understand the present and future workings of the ocean's biogeochemistry. The GEOTRACES strategy is to measure a broad suite of TEIs to constrain the critical biogeochemical processes that influence their distributions. In addition to these "exotic" substances, more traditional properties, including macronutrients (at micromolar and nanomolar levels), CTD, bio-optical parameters, and carbon system characteristics will be measured. The cruise starts at Line W, a repeat hydrographic section southeast of Cape Cod, extends to Bermuda and subsequently through the North Atlantic oligotrophic subtropical gyre, then transects into the African coast in the northern limb of the coastal upwelling region. From there, the cruise goes northward into the Mediterranean outflow. The station locations shown on the map are for the "fulldepth TEI" stations, and constitute approximately half of the stations to be ultimately occupied.</p>
<p><em>Figure 1. The proposed 2010 Atlantic GEOTRACES cruise track plotted on dissolved oxygen at 400 m depth. Data from the World Ocean Atlas (Levitus et al., 2005) were plotted using Ocean Data View (courtesy Reiner Schlitzer). [click on the image to view a larger version]</em><br /><a href="/objectserver/86c47ca3173d90ef5edcec249de05aa6/GEOTRACES_Atl_stas.jpg?url=http%3A%2F%2Fbcodata.whoi.edu%2FUS_GEOTRACES%2FAtlanticSection%2FGEOTRACES_Atl_stas.jpg&f=3163653666383833383364633438646666303638356339386633613165393232687474703a2f2f62636f646174612e77686f692e6564752f55535f47454f5452414345532f41746c616e74696353656374696f6e2f47454f5452414345535f41746c5f737461732e6a7067" target="_blank"><img alt="" src="https://datadocs.bco-dmo.org/d2/US_GEOTRACES/AtlanticSection/GEOTRACES_Atl_stas.jpg" style="width:350px" /></a></p>
<p>Hydrography, CTD and nutrient measurements will be supported by the Ocean Data Facility (J. Swift) at Scripps Institution of Oceanography and funded through NSF Facilities. They will be providing an additional CTD rosette system along with nephelometer and LADCP. A trace metal clean Go-Flo Rosette and winch will be provided by the group at Old Dominion University (G. Cutter) along with a towed underway pumping system.</p>
<p>The North Atlantic Transect cruise began in 2010 with KN199 leg 4 (station sampling) and leg 5 (underway sampling only) (Figure 2).</p>
<p><a href="http://bcodata.whoi.edu//US_GEOTRACES/AtlanticSection/Cruise_Report_for_Knorr_199_Final_v3.pdf" target="_blank">KN199-04 Cruise Report (PDF)</a></p>
<p><em>Figure 2. The red line shows the cruise track for the first leg of the US Geotraces North Atlantic Transect on the R/V Knorr in October 2010. The rest of the stations (beginning with 13) will be completed in October-December 2011 on the R/V Knorr (courtesy of Bill Jenkins, Chief Scientist, GNAT first leg). [click on the image to view a larger version]</em><br /><a href="/objectserver/28028490f13f23fe63a08ff289a7cc8a/GNAT_stationPlan.jpg?url=http%3A%2F%2Fbcodata.whoi.edu%2FUS_GEOTRACES%2FAtlanticSection%2FGNAT_stationPlan.jpg&f=3837393231613337386638616637626663373565643337316231306231363634687474703a2f2f62636f646174612e77686f692e6564752f55535f47454f5452414345532f41746c616e74696353656374696f6e2f474e41545f73746174696f6e506c616e2e6a7067" target="_blank"><img alt="Atlantic Transect Station location map" src="https://datadocs.bco-dmo.org/d2/US_GEOTRACES/AtlanticSection/GNAT_stationPlan_sm.jpg" style="width:350px" /></a></p>
<p>The section completion effort resumed again in November 2011 with KN204-01A,B (Figure 3).</p>
<p><a href="http://bcodata.whoi.edu//US_GEOTRACES/AtlanticSection/Submitted_Preliminary_Cruise_Report_for_Knorr_204-01.pdf" target="_blank">KN204-01A,B Cruise Report (PDF)</a></p>
<p><em>Figure 3. Station locations occupied on the US Geotraces North Atlantic Transect on the R/V Knorr in November 2011. [click on the image to view a larger version]</em><br /><a href="/objectserver/008fd0c21f8a23724741e9c150458427/KN204-01_Stations.png?url=http%3A%2F%2Fbcodata.whoi.edu%2FUS_GEOTRACES%2FAtlanticSection%2FKN204-01_Stations.png&f=6633373930333834633833323933666666326139613061306266336237306638687474703a2f2f62636f646174612e77686f692e6564752f55535f47454f5452414345532f41746c616e74696353656374696f6e2f4b4e3230342d30315f53746174696f6e732e706e67" target="_blank"><img alt="Atlantic Transect/Part 2 Station location map" src="https://datadocs.bco-dmo.org/d2/US_GEOTRACES/AtlanticSection/KN204-01_Stations.png" style="width:350px" /></a></p>
<p>Data from the North Atlantic Transect cruises are available under the Datasets heading below, and consensus values for the SAFe and North Atlantic GEOTRACES Reference Seawater Samples are available from the GEOTRACES Program Office: <a href="http://www.geotraces.org/science/intercalibration/322-standards-and-reference-materials?acm=455_215" target="_blank">Standards and Reference Materials</a></p>
<p><strong>ADCP data</strong> are available from the Currents ADCP group at the University of Hawaii at the links below:<br /><a href="http://currents.soest.hawaii.edu/uhdas_adcp/year2010.html#kn199_4" target="_blank">KN199-04</a> (leg 1 of 2010 cruise; Lisbon to Cape Verde)<br /><a href="http://currents.soest.hawaii.edu/uhdas_adcp/year2010.html#kn199_5" target="_blank">KN199-05</a> (leg 2 of 2010 cruise; Cape Verde to Charleston, NC)<br /><a href="http://currents.soest.hawaii.edu/uhdas_adcp/year2011.html#kn204_01" target="_blank">KN204-01A</a> (part 1 of 2011 cruise; Woods Hole, MA to Bermuda)<br /><a href="http://currents.soest.hawaii.edu/uhdas_adcp/year2011.html#kn204_02" target="_blank">KN204-01B</a> (part 2 of 2011 cruise; Bermuda to Cape Verde)</p>
U.S. GEOTRACES NAT
largerWorkCitation
project
eng; USA
biota
oceans
Subtropical northern Atlantic Ocean
-69.8139
-18.2545
17.3505
39.68403
2010-10-01
2011-11-30
Subtropical western and eastern North Atlantic Ocean (GA03)
0
BCO-DMO catalogue of parameters from Cellular elemental content of individual phytoplankton cells collected during US GEOTRACES North Atlantic Transect cruises in the Subtropical western and eastern North Atlantic Ocean during Oct and Nov, 2010 and Nov. 2011
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/550520.rdf
Name: cruise_id
Units: unitless
Description: cruise identification
http://lod.bco-dmo.org/id/dataset-parameter/550521.rdf
Name: station_GEOTRC
Units: unitless
Description: GEOTRACES station number; ranges from 1 through 12 for KN199-04 and 1 through 24 for KN204-01. Stations 7 and 9 were skipped on KN204-01. Some GeoFish stations are denoted as X_to_Y indicating the tow occurred between stations X and Y. Values were added from the intermediate US GEOTRACES master file (see Processing Description).
http://lod.bco-dmo.org/id/dataset-parameter/550522.rdf
Name: lat
Units: decimal degrees
Description: station latitude; north is positive
http://lod.bco-dmo.org/id/dataset-parameter/550523.rdf
Name: lon
Units: decimal degrees
Description: station longitude; east is postive
http://lod.bco-dmo.org/id/dataset-parameter/550524.rdf
Name: cast_GEOTRC
Units: unitless
Description: Cast identifier; numbered consecutively within a station. Values were added from the intermediate US GEOTRACES master file (see Processing Description).
http://lod.bco-dmo.org/id/dataset-parameter/550525.rdf
Name: event_GEOTRC
Units: unitless
Description: Unique identifying number for US GEOTRACES sampling events; ranges from 2001 to 2225 for KN199-04 events and from 3001 to 3282 for KN204-01 events. Values were added from the intermediate US GEOTRACES master file (see Processing Description).
http://lod.bco-dmo.org/id/dataset-parameter/550526.rdf
Name: depth_GEOTRC_CTD
Units: meters
Description: Observation/sample depth in meters; calculated from CTD pressure. Values were added from the intermediate US GEOTRACES master file (see Processing Description).
http://lod.bco-dmo.org/id/dataset-parameter/550527.rdf
Name: depth_GEOTRC_CTD_round
Units: meters
Description: Rounded observation/sample depth in meters; calculated from CTD pressure. Values were added from the intermediate US GEOTRACES master file (see Processing Description).
http://lod.bco-dmo.org/id/dataset-parameter/550528.rdf
Name: sample_GEOTRC
Units: unitless
Description: Unique identifying number for US GEOTRACES samples; ranges from 5033 to 6078 for KN199-04 and from 6112 to 8148 for KN204-01. PI-supplied values were identical to those in the intermediate US GEOTRACES master file. Originally submitted as 'GEOTRACES #'; this parameter name has been changed to conform to BCO-DMO's GEOTRACES naming conventions.
http://lod.bco-dmo.org/id/dataset-parameter/550529.rdf
Name: sample_bottle_GEOTRC
Units: unitless
Description: Unique identification numbers given to samples taken from bottles; ranges from 1 to 24; often used synonymously with bottle number. Values were added from the intermediate US GEOTRACES master file (see Processing Description).
http://lod.bco-dmo.org/id/dataset-parameter/550530.rdf
Name: bottle_GEOTRC
Units: unitless
Description: Alphanumeric characters identifying bottle type (e.g. NIS representing Niskin and GF representing GOFLO) and position on a CTD rosette. Values were added from the intermediate US GEOTRACES master file (see Processing Description).
http://lod.bco-dmo.org/id/dataset-parameter/550531.rdf
Name: project
Units: unitless
Description: GEOTRACES project: North Atlantic Zonal Transect
http://lod.bco-dmo.org/id/dataset-parameter/550532.rdf
Name: grid_type
Units: unitless
Description: grid type: plankton samples were mounted onto either gold (Au) or aluminum (Al) electron microscopy grids
http://lod.bco-dmo.org/id/dataset-parameter/550533.rdf
Name: grid_num
Units: unitless
Description: GEOTRACES bottle number followed by an internal designation for the grid
http://lod.bco-dmo.org/id/dataset-parameter/550534.rdf
Name: SXRF_run
Units: unitless
Description: samples were analyzed by synchrotron x-ray fluorescence (SXRF) during two analytical runs in July 2011 (2011r2) or August 2012 (2012r2).
http://lod.bco-dmo.org/id/dataset-parameter/550535.rdf
Name: mda_id
Units: unitless
Description: unique identifier given to each SXRF scan during each run
http://lod.bco-dmo.org/id/dataset-parameter/550536.rdf
Name: cell_type
Units: unitless
Description: each cell was classified as either an autotrophic flagellate (Aflag); autotrophic picoplankter (Apico); or a diatom.
http://lod.bco-dmo.org/id/dataset-parameter/550537.rdf
Name: cell_vol
Units: um^3
Description: biovolume of each cell estimated from microscope measurements of cell dimensions
http://lod.bco-dmo.org/id/dataset-parameter/550538.rdf
Name: cell_C
Units: mol/cell
Description: cellular C content calculated from biovolume using the relationships of Menden-Deuer & Lessard (2000)
http://lod.bco-dmo.org/id/dataset-parameter/550539.rdf
Name: cell_Si
Units: mol/cell
Description: total elemental Si content of each cell was measured with SXRF. Details provided in Twining et al. (2015).
http://lod.bco-dmo.org/id/dataset-parameter/550540.rdf
Name: cell_P
Units: mol/cell
Description: total elemental P content of each cell was measured with SXRF. Details provided in Twining et al. (2015).
http://lod.bco-dmo.org/id/dataset-parameter/550541.rdf
Name: cell_S
Units: mol/cell
Description: total elemental S content of each cell was measured with SXRF. Details provided in Twining et al. (2015).
http://lod.bco-dmo.org/id/dataset-parameter/550542.rdf
Name: cell_Mn
Units: mol/cell
Description: total elemental Mn content of each cell was measured with SXRF. Details provided in Twining et al. (2015).
http://lod.bco-dmo.org/id/dataset-parameter/550543.rdf
Name: cell_Fe
Units: mol/cell
Description: total elemental Fe content of each cell was measured with SXRF. Details provided in Twining et al. (2015).
http://lod.bco-dmo.org/id/dataset-parameter/550544.rdf
Name: cell_Co
Units: mol/cell
Description: total elemental Co content of each cell was measured with SXRF. Details provided in Twining et al. (2015).
http://lod.bco-dmo.org/id/dataset-parameter/550545.rdf
Name: cell_Ni
Units: mol/cell
Description: total elemental Ni content of each cell was measured with SXRF. Details provided in Twining et al. (2015).
http://lod.bco-dmo.org/id/dataset-parameter/550546.rdf
Name: cell_Cu
Units: mol/cell
Description: total elemental Cu content of each cell was measured with SXRF. Details provided in Twining et al. (2015).
http://lod.bco-dmo.org/id/dataset-parameter/550547.rdf
Name: cell_Zn
Units: mol/cell
Description: total elemental Zn content of each cell was measured with SXRF. Details provided in Twining et al. (2015).
http://lod.bco-dmo.org/id/dataset-parameter/550548.rdf
Name: light_image_filename
Units: unitless
Description: light image filename
http://lod.bco-dmo.org/id/dataset-parameter/550549.rdf
Name: chl_image_filename
Units: unitless
Description: Chl image filename
http://lod.bco-dmo.org/id/dataset-parameter/550550.rdf
Name: SXRF_map_filename
Units: unitless
Description: SXRF map filename
http://lod.bco-dmo.org/id/dataset-parameter/550551.rdf
Name: SXRF_spectrum_filename
Units: unitless
Description: SXRF spectrum filename
http://lod.bco-dmo.org/id/dataset-parameter/550552.rdf
Name: BTL_ISO_DateTime_UTC
Units: yyyy-MM-dd'T'HH:mm:ss.SS'Z'
Description: Date and time (UTC) variable recorded at the bottle sampling time in ISO compliant format. Values were added from the intermediate US GEOTRACES master file (see Processing Description). This standard is based on ISO 8601:2004(E) and takes on the following form: 2009-08-30T14:05:00[.xx]Z (UTC time)
http://lod.bco-dmo.org/id/dataset-parameter/564854.rdf
Name: sta_PI
Units: unitless
Description: station number given by PI
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
https://doi.org/10.1575/1912/bco-dmo.641155
download
onLine
dataset
<p>SXRF samples were prepared from unfiltered water taken from GEOTRACES GO-Flo bottles at the shallowest depth and deep chlorophyll maximum. Cells were preserved with 0.25% trace-metal clean buffered glutaraldehyde and centrifuged onto C/formvar-coated Au TEM grids. Grids were briefly rinsed with a drop of ultrapure water and dried in a Class-100 cabinet. SXRF analysis was performed using the 2-ID-E beamline at the Advanced Photon source (Argonne National Laboratory) following the protocols of Twining et al. (2011).</p>
Specified by the Principal Investigator(s)
<p>Data were processed as described in Twining et al. (2015)</p>
<p>Between 9 and 20 cells were analyzed from the shallowest bottle and deep chlorophyll maximum at the subset of stations. The elemental content of each cell has been corrected for elements contained in the carbon substrate. Trace element concentrations are presented as mmol/mol P. Geometric mean concentrations (+/- standard error of the mean) are presented, along with the number of cells analyzed.</p>
<p><strong>BCO-DMO Processing:</strong></p>
<p>- added conventional header with dataset name, PI name, version date<br />
- renamed parameters to BCO-DMO standard<br />
- replaced blank cells with nd<br />
- sorted by cruise, station, grid#<br />
- changed station 99 and 153 to cruise AT199-05 from AT199-04<br />
- revised station 9 depths to match master events log</p>
<p>With the agreement of BODC and the US GEOTRACES lead PIs, BCO-DMO added standard US GEOTRACES information, such as the US GEOTRACES event number. To accomplish this, BCO-DMO compiled a 'master' dataset composed of the following parameters: station_GEOTRC, cast_GEOTRC (bottle and pump data only), event_GEOTRC, sample_GEOTRC, sample_bottle_GEOTRC (bottle data only), bottle_GEOTRC (bottle data only), depth_GEOTRC_CTD (bottle data only), depth_GEOTRC_CTD_rounded (bottle data only), BTL_ISO_DateTime_UTC (bottle data only), and GeoFish_id (GeoFish data only). This added information will facilitate subsequent analysis and inter comparison of the datasets.</p>
<p>Bottle parameters in the master file were taken from the GT-C_Bottle_GT10, GT-C_Bottle_GT11, ODF_Bottle_GT10, and ODF_Bottle_GT11 datasets. Non-bottle parameters, including those from GeoFish tows, Aerosol sampling, and McLane Pumps, were taken from the Event_Log_GT10 and Event_Log_GT11 datasets. McLane pump cast numbers missing in event logs were taken from the Particulate Th-234 dataset submitted by Ken Buesseler.</p>
<p>A standardized BCO-DMO method (called "join") was then used to merge the missing parameters to each US GEOTRACES dataset, most often by matching on sample_GEOTRC or on some unique combination of other parameters.</p>
<p>If the master parameters were included in the original data file and the values did not differ from the master file, the original data columns were retained and the names of the parameters were changed from the PI-submitted names to the standardized master names. If there were differences between the PI-supplied parameter values and those in the master file, both columns were retained. If the original data submission included all of the master parameters, no additional columns were added, but parameter names were modified to match the naming conventions of the master file.</p>
<p>See the dataset parameters documentation for a description of which parameters were supplied by the PI and which were added via the join method.</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
PI Supplied Instrument Name: Instrument Name: GO-FLO Bottle Instrument Short Name:GO-FLO Instrument Description: GO-FLO bottle cast used to collect water samples for pigment, nutrient, plankton, etc. The GO-FLO sampling bottle is specially designed to avoid sample contamination at the surface, internal spring contamination, loss of sample on deck (internal seals), and exchange of water from different depths. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/30/
PI Supplied Instrument Name: Instrument Name: GeoFish Towed near-Surface Sampler Instrument Short Name:GeoFish Instrument Description: The GeoFish towed sampler is a custom designed near surface ( Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/31/
PI Supplied Instrument Name: Instrument Name: Microscope - Optical Instrument Short Name: Instrument Description: Instruments that generate enlarged images of samples using the phenomena of reflection and absorption of visible light. Includes conventional and inverted instruments. Also called a "light microscope". Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB05/
PI Supplied Instrument Name: PI Supplied Instrument Description:SXRF analysis was performed on the 2-ID-E beamline at the Advanced Photon source (Argonne National Laboratory). The synchetron consists of a storage ring which produces high energy electromagnetic radiation. X-rays diverted to the 2-ID-E beamline are used for x-ray fluorescence mapping of biological samples. X-rays were tuned to an energy of 10 keV to enable the excition of K-alpha fluorescence for the elements reported. The beam is focused using Fresnel zoneplates to achieve high spatial resolution; for our application a focused spot size of 0.5um was used. A single element germanium energy dispersive detector is used to record the X-ray fluorescence spectrum. Instrument Name: X-ray fluorescence analyzer Instrument Short Name:XRF analyzer Instrument Description: Instruments that identify and quantify the elemental constituents of a sample from the spectrum of electromagnetic radiation emitted by the atoms in the sample when excited by X-ray radiation.
Cruise: KN199-04
KN199-04
R/V Knorr
Community Standard Description
International Council for the Exploration of the Sea
R/V Knorr
vessel
KN199-04
William J. Jenkins
Woods Hole Oceanographic Institution
http://bcodata.whoi.edu/US_GEOTRACES/AtlanticSection/Cruise_Report_for_Knorr_199_Final_v3.pdf
Report describing KN199-04
Cruise: KN199-05
KN199-05
R/V Knorr
Community Standard Description
International Council for the Exploration of the Sea
R/V Knorr
vessel
KN199-05
William J. Jenkins
Woods Hole Oceanographic Institution
Cruise: KN204-01
KN204-01
R/V Knorr
Community Standard Description
International Council for the Exploration of the Sea
R/V Knorr
vessel
KN204-01
Edward A. Boyle
Massachusetts Institute of Technology
http://bcodata.whoi.edu/US_GEOTRACES/AtlanticSection/STS_Prelim_GT11_Doc.pdf
Report describing KN204-01
R/V Knorr
Community Standard Description
International Council for the Exploration of the Sea
R/V Knorr
vessel