http://lod.bco-dmo.org/id/dataset/551060
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2015-02-17
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Coral skeletal carbon and oxygen isotopes; measured photosynthesis to respiration ratios; isotope-based photosynthesis to respiration ratios from reef field sites in Puerto Morelos, Mexico & Kaneohe Bay, Hawaii
2015-02-17
publication
2015-02-17
revision
BCO-DMO Linked Data URI
2015-02-17
creation
http://lod.bco-dmo.org/id/dataset/551060
Andréa G. Grottoli
Ohio State University
principalInvestigator
Mark E. Warner
University of Delaware
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Grottoli, A. G., Warner, M. E. (2015) Coral skeletal carbon and oxygen isotopes; measured photosynthesis to respiration ratios; isotope-based photosynthesis to respiration ratios from reef field sites in Puerto Morelos, Mexico & Kaneohe Bay, Hawaii. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 17 Feb 2015) Version Date 2015-02-17 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/551060 [access date]
Coral skeletal carbon and oxygen isotopes; measured photosynthesis to respiration ratios; isotope-based photosynthesis to respiration ratios. Dataset Description: <p>Coral original and corrected skeletal carbon and oxygen isotopes; original and corrected measured photosynthesis to respiration ratios; original and corrected isotope-based photosynthesis to respiration ratios. <em>Orbicella faveolata, Porites astreoides</em>, and <em>Porites divaricata</em> were collected at Puerto Morelos, Mexico (20°50'N, 86°52'W)<em>. Porites compressa, Montipora capitata, </em>and<em> Porites lobata </em>were collected at Kaneohe Bay, Hawaii (21°26.18'N, 157°47.56'W).</p> Methods and Sampling: <p>Full details of the experimental design and analytical methods are in:<br />
Schoepf V, Levas SJ, Rodrigues LJ, McBride M, Aschaffenburg MD, Matsui Y, Warner ME, Hughes AD, Grottoli AG. 2014. Kinetic and metabolic isotope effects in coral skeletal carbon isotopes: A re-evaluation using experimental coral bleaching as a case study. Geochimica et Cosmochimica Acta, 146: 164-178. doi:<a href="http://dx.doi.org/10.1016/j.gca.2014.09.033" target="_blank">10.1016/j.gca.2014.09.033</a></p>
<p>A brief description of the methods follows:</p>
<p><strong>Pacific coral bleaching experiments</strong><br />
A detailed description of the first bleaching experiment in Hawaii can be found in Rodrigues and Grottoli (2006). Briefly, coral fragments from branching <em>Porites compressa</em> and branching <em>Montipora capitata</em> were collected from Point Reef, Kaneohe Bay, Hawaii in late August 2003 from 2 m depth. After allowing them to acclimate for two weeks, half of all fragments were placed in shaded outdoor tanks with ambient seawater (26.8 degrees C +/- 0.04 SE) (non-bleached controls), while the other half were placed in tanks with elevated temperature seawater (30.1 degrees C +/- 0.05 SE) (bleached corals). Temperature was gradually elevated over the course of three days. Corals were not fed during the experiment, and inflow pipes were fitted with a 50 um-filter. To minimize positional effects, corals were rotated within and among tanks of the same treatment daily. After one month, 25% of all treatment and control fragments were collected and frozen for isotopic analyses (= 0 month recovery), whereas the remaining corals were placed back on the reef to recover <em>in situ</em>. To assess short and long term recovery, a third of all remaining treatment and control corals were collected after 1.5, 4, and 8 months, respectively. A second, similar bleaching experiment was performed in summer 2006 to assess bleaching impacts on mounding <em>Porites lobata</em>.</p>
<p><strong>Caribbean coral bleaching experiment</strong><br />
Coral fragments of mounding <em>Orbicella faveolata</em> (formerly <em>Montastraea faveolata</em>), encrusting to mounding <em>Porites astreoides</em>, and branching <em>Porites divaricata</em> were collected in July 2009 from shallow reefs (3–8 m) near Puerto Morelos Reefs National Park, Mexico. After allowing them to acclimate for 5 days, half of the coral fragments were placed in tanks with ambient seawater temperature (30.66 +/- 0.24 degrees C) (nonbleached controls), while the other half were placed in tanks with elevated temperature seawater (31.48 +/- 0.20 degrees C) (bleached corals). Seawater temperature in the treatment tanks was gradually elevated over the course of seven days. Corals were not fed, but had access to unfiltered seawater. Fragments were rotated daily within and among tanks of the same treatment to minimize any positional effects. After a total of 15 days, temperature in all tanks was returned to ambient levels, and all coral fragments were placed on the back reef to recover<em> in situ </em>for one full year. In July 2010, the bleaching treatment was repeated using the same experimental protocol. To assess short- and long-term recovery from repeat bleaching, one fragment from each colony and treatment was recollected from the reef after 1.5 and 11 months of recovery and then frozen for isotopic analyses.</p>
<p><strong>Photosynthesis to respiration ratios</strong><br />
Net photosynthesis (P) and day respiration (R) rates were measured by quantifying changes in dissolved oxygen by incubating non-bleached and bleached corals in UV-transparent acrylic chambers under light and dark conditions. Refer to Schoepf et al. 2014 Geochimica et Cosmochimica Acta (doi:<a href="http://dx.doi.org/10.1016/j.gca.2014.09.033" target="_blank">10.1016/j.gca.2014.09.033</a>) for equations used in calculations (equation 1).</p>
<p><strong>Isotopic analyses</strong><br />
<strong>Seawater dissolved inorganic carbon (DIC) isotopes</strong>: A total of nine filtered seawater samples from Kaneohe Bay, Hawaii, were collected throughout 2006/07 for d13CDIC analyses. They were preserved with anhydrous HgCl. In the laboratory, each sample was acidified on a vacuum extraction line under high-purity helium flow, with the resulting CO2 gas cryogenically isolated under vacuum, and the DIC concentration was determined. The CO2 from each DIC sample was sealed in Pyrex ampoules and introduced into a Finnigan Delta IV Stable Isotope Ratio Mass Spectrometer (SIRMS) via an automated 10-port inlet. All d13C values were reported as per mil values relative to Vienna-Pee Dee Belemnite limestone standard (v-PDB). d13CDIC analyses were not performed for seawater from Puerto Morelos, Mexico.</p>
<p><strong>Tissue and skeletal isotopes: </strong>A detailed description of the isotopic analyses for the Pacific corals can be found in Rodrigues and Grottoli (2006) and Levas et al. (2013), and for the Caribbean corals in Schoepf (2013).</p>
<p><strong>Data correction: </strong>Coral skeletal carbon isotopes (d13Csorig) were corrected (d13Cscorr) using skeletal oxygen isotopes (d18Os) to remove kinetic effects according to the equation developed by Heikoop et al. (2000). Refer to equation 2 of Schoepf et al. 2014 Geochimica et Cosmochimica Acta (doi:<a href="http://dx.doi.org/10.1016/j.gca.2014.09.033" target="_blank">10.1016/j.gca.2014.09.033</a>).</p>
<p><strong>Carbon isotopic equilibrium</strong>: Carbon isotopic equilibrium (d13Ceq) for aragonite was calculated following the precedence of McConnaughey et al. (1997) and Heikoop et al. (2000) using the equation of Romanek et al. (1992). Refer to equation 3 of Schoepf et al. 2014 Geochimica et Cosmochimica Acta (doi:<a href="http://dx.doi.org/10.1016/j.gca.2014.09.033" target="_blank">10.1016/j.gca.2014.09.033</a>).</p>
<p><strong>Oxygen isotopic equilibrium</strong>: Two different methods, Grossman and Ku (1986) and Maier (2004), exist in the literature to calculate oxygen isotopic equilibrium in carbonates (d18Oeq), where only Grossman and Ku (1986) incorporate temperature-dependent fractionation. Both methods were used in this study. Refer to equations 4 and 5 of Schoepf et al. 2014 Geochimica et Cosmochimica Acta (doi:<a href="http://dx.doi.org/10.1016/j.gca.2014.09.033" target="_blank">10.1016/j.gca.2014.09.033</a>).</p>
<p><strong>Isotope-based P/R ratios</strong>: P/R ratios were calculated from skeletal and tissue isotopes according following the equations of Maier (2004) and Kaandorp et al. (2005). Refer to equations 6 and 7 of Schoepf et al. 2014 Geochimica et Cosmochimica Acta (doi:<a href="http://dx.doi.org/10.1016/j.gca.2014.09.033" target="_blank">10.1016/j.gca.2014.09.033</a>).</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-0825413 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=0825413
completed
Andréa G. Grottoli
Ohio State University
614-292-5782
School of Earth Sciences 125 South Oval Mall
Columbus
OH
43210
United States
grottoli.1@osu.edu
pointOfContact
Mark E. Warner
University of Delaware
302-645-4365
School of Marine Science and Policy 700 Pilottown Rd.
Lewes
DE
19958
USA
mwarner@udel.edu
pointOfContact
asNeeded
Dataset Version: 17 Feb 2015
Unknown
region
site_name
lat
lon
species
recovery_mos
treatment_type
genotype
delta_13C_s_orig
delta_18O_s_orig
delta_13C_s_corr
P_to_R_ratio_meas
P_to_R_ratio_orig_Grossman
P_to_R_ratio_orig_Maier
P_to_R_ratio_corr_Grossman
P_to_R_ratio_corr_Maier
Finnigan Delta IV Stable Isotope Ratio Mass Spectrometer
theme
None, User defined
region
No BCO-DMO term
latitude
longitude
species scientific name (binomial)
treatment
featureType
BCO-DMO Standard Parameters
Isotope-ratio Mass Spectrometer
instrument
BCO-DMO Standard Instruments
Coral_physiology_field_exper
service
Deployment Activity
Puerto Morelos, Mexico & Kaneohe Bay, Hawaii
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Physiology and Biogeochemistry of Repeatedly Bleached and Recovering Caribbean Corals
https://osprey.bco-dmo.org/project/516103
Physiology and Biogeochemistry of Repeatedly Bleached and Recovering Caribbean Corals
<p><em>(Extracted from the NSF award abstract)</em></p>
<p>The overall stability and health of coral reefs is declining world-wide at an unprecedented rate. Mass coral bleaching, wherein exposure to elevated temperature leads to the loss of significant numbers of endosymbiotic dinoflagellates (Symbiodinium spp., commonly called zooxanthellae) and/or photosynthetic pigments, serves as a primary global example of how fragile this symbiosis is. While we have begun to understand the ecological and physiological impacts of bleaching, there remain key fundamental gaps in knowledge. In particular, it is becoming increasingly clear that a) not all corals either respond to, or recover from, bleaching events the same way, and that b) the impact of annual or repeated bleaching events on corals has not been examined in sufficient detail. Several non-mutually exclusive ecological and physiological pathways could impact how a particular coral species succumbs to or recovers from bleaching. Recent evidence suggests that the following features may play key roles for coral survival in the face of future seawater warming and mass bleaching events: 1) shifts in trophic partitioning (e.g., proportional reliance on autotrophy and heterotrophy) and energy reserve utilization, 2) enhanced thermal tolerance through host and algal-mediated physiological responses, and 3) harboring of different Symbiodinium phylotypes. However, these mechanisms have yet to be investigated in a unified approach that covers the entire coral holobiont system (algae, host tissue, and skeleton), or under scenarios of repeated bleaching.</p>
<p>The overall objectives of this study are as follows: 1) to determine the effect of single and repeated bleaching on the physiology, biogeochemistry, and recovery of some Caribbean coral species, and 2) to determine which Symbiodinium-type and host-species combinations are more resilient to single and repeated bleaching, what aspects of their physiology and biogeochemistry render them resilient, and to use this information to evaluate the long-term persistence of Caribbean coral reefs. To address these objectives, the following physiological variables will be measured: 1) Symbiodinium type, photochemical function and algal stress physiology, and 2) animal host energy reserves, defense enzyme concentration, skeletal growth, and feeding capacity in the corals Porites porites, Porites astreoides, and Montastraea faveolata. Corals will be examined immediately following thermal stress designed to approximate natural bleaching, and recovery will be monitored over short and long-term time scales. Next, the impact of repeated bleaching will be examined in the subsequent year, followed by examination over the next recovery period. This research is designed to simultaneously evaluate the symbiotic algae, coral host, and skeleton, and to identify patterns of physiological responses and recovery of each Symbiodinium-type and host-species combination that would be indicative of the resilience capacity of Caribbean corals to future more frequent thermal perturbations.</p>
repeat coral bleaching
largerWorkCitation
project
eng; USA
biota
oceans
Puerto Morelos, Mexico & Kaneohe Bay, Hawaii
2015-02-17
Puerto Morelos, Mexico
0
BCO-DMO catalogue of parameters from Coral skeletal carbon and oxygen isotopes; measured photosynthesis to respiration ratios; isotope-based photosynthesis to respiration ratios from reef field sites in Puerto Morelos, Mexico & Kaneohe Bay, Hawaii
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/551122.rdf
Name: region
Units: unitless
Description: <p>Name of the region where samples were collected.</p>
http://lod.bco-dmo.org/id/dataset-parameter/551123.rdf
Name: site_name
Units: unitless
Description: <p>Name of the site where samples were collected.</p>
http://lod.bco-dmo.org/id/dataset-parameter/551124.rdf
Name: lat
Units: decimal degrees
Description: <p>Latitude of the collection site. Positive values = North.</p>
http://lod.bco-dmo.org/id/dataset-parameter/551125.rdf
Name: lon
Units: decimal degrees
Description: <p>Longitude of the collection site. Positive values = East.</p>
http://lod.bco-dmo.org/id/dataset-parameter/551126.rdf
Name: species
Units: unitless
Description: <p>Name of the coral species.</p>
http://lod.bco-dmo.org/id/dataset-parameter/551127.rdf
Name: recovery_mos
Units: months
Description: <p>Recovery time (in months).</p>
http://lod.bco-dmo.org/id/dataset-parameter/551128.rdf
Name: treatment_type
Units: unitless
Description: <p>Treatment type.</p>
http://lod.bco-dmo.org/id/dataset-parameter/551129.rdf
Name: genotype
Units: unitless
Description: <p>Genotype (parent colony).</p>
http://lod.bco-dmo.org/id/dataset-parameter/551130.rdf
Name: delta_13C_s_orig
Units: per mil (‰)
Description: <p>The original coral skeletal carbon isotopic composition in permil relative to the standard v-PDB.</p>
http://lod.bco-dmo.org/id/dataset-parameter/551131.rdf
Name: delta_18O_s_orig
Units: per mil (‰)
Description: <p>The original coral skeletal oxygen isotopic composition in permil relative to the standard v-PDB.</p>
http://lod.bco-dmo.org/id/dataset-parameter/551132.rdf
Name: delta_13C_s_corr
Units: per mil (‰)
Description: <p>The corrected coral skeletal carbon isotopic composition in permil relative to the standard v-PDB.</p>
http://lod.bco-dmo.org/id/dataset-parameter/551133.rdf
Name: P_to_R_ratio_meas
Units: unitless (ratio)
Description: <p>The photosynthesis to respiration ratio measured directly via respirometry.</p>
http://lod.bco-dmo.org/id/dataset-parameter/551134.rdf
Name: P_to_R_ratio_orig_Grossman
Units: unitless (ratio)
Description: <p>Tthe P/R ratio calculated from original isotopes using d18Oeq from Grossman and Ku (1986).</p>
http://lod.bco-dmo.org/id/dataset-parameter/551135.rdf
Name: P_to_R_ratio_orig_Maier
Units: unitless (ratio)
Description: <p>The P/R ratio calculated from original isotopes using d18Oeq from Maier (2004).</p>
http://lod.bco-dmo.org/id/dataset-parameter/551136.rdf
Name: P_to_R_ratio_corr_Grossman
Units: unitless (ratio)
Description: <p>The P/R ratio calculated from corrected isotopes using d18Oeq from Grossman and Ku (1986).</p>
http://lod.bco-dmo.org/id/dataset-parameter/551137.rdf
Name: P_to_R_ratio_corr_Maier
Units: unitless (ratio)
Description: <p>The P/R ratio calculated from corrected isotopes using d18Oeq from Maier (2004).</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
37799
https://datadocs.bco-dmo.org/file/vmmwMWBFEqZ6xP/coral_skel_isotopes.csv
coral_skel_isotopes.csv
Primary data file for dataset ID 551060
download
https://osprey.bco-dmo.org/dataset/551060/data/download
download
onLine
dataset
<p>Full details of the experimental design and analytical methods are in:<br />
Schoepf V, Levas SJ, Rodrigues LJ, McBride M, Aschaffenburg MD, Matsui Y, Warner ME, Hughes AD, Grottoli AG. 2014. Kinetic and metabolic isotope effects in coral skeletal carbon isotopes: A re-evaluation using experimental coral bleaching as a case study. Geochimica et Cosmochimica Acta, 146: 164-178. doi:<a href="http://dx.doi.org/10.1016/j.gca.2014.09.033" target="_blank">10.1016/j.gca.2014.09.033</a></p>
<p>A brief description of the methods follows:</p>
<p><strong>Pacific coral bleaching experiments</strong><br />
A detailed description of the first bleaching experiment in Hawaii can be found in Rodrigues and Grottoli (2006). Briefly, coral fragments from branching <em>Porites compressa</em> and branching <em>Montipora capitata</em> were collected from Point Reef, Kaneohe Bay, Hawaii in late August 2003 from 2 m depth. After allowing them to acclimate for two weeks, half of all fragments were placed in shaded outdoor tanks with ambient seawater (26.8 degrees C +/- 0.04 SE) (non-bleached controls), while the other half were placed in tanks with elevated temperature seawater (30.1 degrees C +/- 0.05 SE) (bleached corals). Temperature was gradually elevated over the course of three days. Corals were not fed during the experiment, and inflow pipes were fitted with a 50 um-filter. To minimize positional effects, corals were rotated within and among tanks of the same treatment daily. After one month, 25% of all treatment and control fragments were collected and frozen for isotopic analyses (= 0 month recovery), whereas the remaining corals were placed back on the reef to recover <em>in situ</em>. To assess short and long term recovery, a third of all remaining treatment and control corals were collected after 1.5, 4, and 8 months, respectively. A second, similar bleaching experiment was performed in summer 2006 to assess bleaching impacts on mounding <em>Porites lobata</em>.</p>
<p><strong>Caribbean coral bleaching experiment</strong><br />
Coral fragments of mounding <em>Orbicella faveolata</em> (formerly <em>Montastraea faveolata</em>), encrusting to mounding <em>Porites astreoides</em>, and branching <em>Porites divaricata</em> were collected in July 2009 from shallow reefs (3–8 m) near Puerto Morelos Reefs National Park, Mexico. After allowing them to acclimate for 5 days, half of the coral fragments were placed in tanks with ambient seawater temperature (30.66 +/- 0.24 degrees C) (nonbleached controls), while the other half were placed in tanks with elevated temperature seawater (31.48 +/- 0.20 degrees C) (bleached corals). Seawater temperature in the treatment tanks was gradually elevated over the course of seven days. Corals were not fed, but had access to unfiltered seawater. Fragments were rotated daily within and among tanks of the same treatment to minimize any positional effects. After a total of 15 days, temperature in all tanks was returned to ambient levels, and all coral fragments were placed on the back reef to recover<em> in situ </em>for one full year. In July 2010, the bleaching treatment was repeated using the same experimental protocol. To assess short- and long-term recovery from repeat bleaching, one fragment from each colony and treatment was recollected from the reef after 1.5 and 11 months of recovery and then frozen for isotopic analyses.</p>
<p><strong>Photosynthesis to respiration ratios</strong><br />
Net photosynthesis (P) and day respiration (R) rates were measured by quantifying changes in dissolved oxygen by incubating non-bleached and bleached corals in UV-transparent acrylic chambers under light and dark conditions. Refer to Schoepf et al. 2014 Geochimica et Cosmochimica Acta (doi:<a href="http://dx.doi.org/10.1016/j.gca.2014.09.033" target="_blank">10.1016/j.gca.2014.09.033</a>) for equations used in calculations (equation 1).</p>
<p><strong>Isotopic analyses</strong><br />
<strong>Seawater dissolved inorganic carbon (DIC) isotopes</strong>: A total of nine filtered seawater samples from Kaneohe Bay, Hawaii, were collected throughout 2006/07 for d13CDIC analyses. They were preserved with anhydrous HgCl. In the laboratory, each sample was acidified on a vacuum extraction line under high-purity helium flow, with the resulting CO2 gas cryogenically isolated under vacuum, and the DIC concentration was determined. The CO2 from each DIC sample was sealed in Pyrex ampoules and introduced into a Finnigan Delta IV Stable Isotope Ratio Mass Spectrometer (SIRMS) via an automated 10-port inlet. All d13C values were reported as per mil values relative to Vienna-Pee Dee Belemnite limestone standard (v-PDB). d13CDIC analyses were not performed for seawater from Puerto Morelos, Mexico.</p>
<p><strong>Tissue and skeletal isotopes: </strong>A detailed description of the isotopic analyses for the Pacific corals can be found in Rodrigues and Grottoli (2006) and Levas et al. (2013), and for the Caribbean corals in Schoepf (2013).</p>
<p><strong>Data correction: </strong>Coral skeletal carbon isotopes (d13Csorig) were corrected (d13Cscorr) using skeletal oxygen isotopes (d18Os) to remove kinetic effects according to the equation developed by Heikoop et al. (2000). Refer to equation 2 of Schoepf et al. 2014 Geochimica et Cosmochimica Acta (doi:<a href="http://dx.doi.org/10.1016/j.gca.2014.09.033" target="_blank">10.1016/j.gca.2014.09.033</a>).</p>
<p><strong>Carbon isotopic equilibrium</strong>: Carbon isotopic equilibrium (d13Ceq) for aragonite was calculated following the precedence of McConnaughey et al. (1997) and Heikoop et al. (2000) using the equation of Romanek et al. (1992). Refer to equation 3 of Schoepf et al. 2014 Geochimica et Cosmochimica Acta (doi:<a href="http://dx.doi.org/10.1016/j.gca.2014.09.033" target="_blank">10.1016/j.gca.2014.09.033</a>).</p>
<p><strong>Oxygen isotopic equilibrium</strong>: Two different methods, Grossman and Ku (1986) and Maier (2004), exist in the literature to calculate oxygen isotopic equilibrium in carbonates (d18Oeq), where only Grossman and Ku (1986) incorporate temperature-dependent fractionation. Both methods were used in this study. Refer to equations 4 and 5 of Schoepf et al. 2014 Geochimica et Cosmochimica Acta (doi:<a href="http://dx.doi.org/10.1016/j.gca.2014.09.033" target="_blank">10.1016/j.gca.2014.09.033</a>).</p>
<p><strong>Isotope-based P/R ratios</strong>: P/R ratios were calculated from skeletal and tissue isotopes according following the equations of Maier (2004) and Kaandorp et al. (2005). Refer to equations 6 and 7 of Schoepf et al. 2014 Geochimica et Cosmochimica Acta (doi:<a href="http://dx.doi.org/10.1016/j.gca.2014.09.033" target="_blank">10.1016/j.gca.2014.09.033</a>).</p>
Specified by the Principal Investigator(s)
<p>All samples of <em>Orbicella faveolata, Porites astreoides, Porites divaricata</em> and <em>Porites lobata</em> were processed at The Ohio State University, Columbus, OH, USA. All samples of <em>Porites compressa</em> and <em>Montipora capitata</em> were processed at the University of Pennsylvania, Philadelphia, PA, USA.</p>
<p>Details of the statistical analysis methods are in Schoepf et al. 2014 Geochimica et Cosmochimica Acta (doi:<a href="http://dx.doi.org/10.1016/j.gca.2014.09.033" target="_blank">10.1016/j.gca.2014.09.033</a>).</p>
<p>There were three outliers not included in the final analysis; those values have been replaced with 'nd' in the dataset.</p>
<p>BCO-DMO edits:<br />
- Modified parameter names to conform with BCO-DMO naming conventions.<br />
- Replaced spaces with underscores in the species column.<br />
- Replaced '.' with 'nd' to indicate 'no data'.<br />
- Added lat/lon of collection site from the metadata form.</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Finnigan Delta IV Stable Isotope Ratio Mass Spectrometer
Finnigan Delta IV Stable Isotope Ratio Mass Spectrometer
PI Supplied Instrument Name: Finnigan Delta IV Stable Isotope Ratio Mass Spectrometer PI Supplied Instrument Description:The CO2 from each DIC sample was sealed in Pyrex ampoules and introduced into a Finnigan Delta IV Stable Isotope Ratio Mass Spectrometer (SIRMS). Instrument Name: Isotope-ratio Mass Spectrometer Instrument Short Name:IR Mass Spec; IRMS Instrument Description: The Isotope-ratio Mass Spectrometer is a particular type of mass spectrometer used to measure the relative abundance of isotopes in a given sample (e.g. VG Prism II Isotope Ratio Mass-Spectrometer). Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB16/
Deployment: Coral_physiology_field_exper
Coral_physiology_field_exper
Reef Field Sites
laboratory
Coral_physiology_field_exper
Andréa G. Grottoli
Ohio State University
Reef Field Sites
laboratory