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Samples were collected by push cores along a transect from the hottest part of the venting area towards the unaffected, colder sediments, i.e., at 0 cm, 50 cm, 100 cm, 150 cm, 200 cm, and 300 cm. On shore, the push cores were sliced in 2 cm intervals, frozen on dry ice, and subsequently stored at -80\u00baC. DNA was extracted using a commercially available extraction kit. We were able to successfully sequence 16S rRNA amplicons for Bacteria and Archaea from a total of 26 samples. Sequence data are currently being analyzed and will be deposited in GenBank prior to publication and will be made available to the scientific community. For TOC and TON analyses, dried sediment samples were weighed into methanol rinsed silver boats (4 x 6 mm, Costech). 96 well glass plates (combusted 4 hrs @ 450C) holding these samples were placed in a vacuum desiccator that also contained an open dish with about 50 ml fresh, concentrated (12N) HCl. An inverted crystallization dish was placed over the samples to protect them from water that can condense and rain down from the desiccator top during heating. The desiccator was closed and pumped out with an air driven aspirator, to a reading of about ~0.5 atm and the desiccator is placed in an oven kept between 60 and 65 C. Acidification was allowed to run for 60 to 72 hours. The samples were then transferred to another vacuum desiccator, this time charged with indicating silica gel (Fisher S162-500, activated by heating to 450\u00baC overnight, and pumped down again and dried for about 24 hours before use. Immediately before analysis, samples are wrapped in tin boats (Costech, 4x6, methanol rinsed).\u00a0<\/p><\/div>","@type":"rdf:HTML"}],"http:\/\/ocean-data.org\/schema\/hasBriefDescription":[{"@value":"Organic carbon and nitrogen in near-vent sediment samples, Paleochori Bay, Milos Island, Greece","@language":"en-US"}],"http:\/\/purl.org\/dc\/terms\/description":[{"@value":"
This dataset describes the sediment push cores taken by Scuba diving at a shallow-water hydrothermal vent site in Paleochori Bay, Milos Island, Greece for subsequent analyses to determine the total organic carbon (TOC), total organic nitrogen (TON), the stable carbon isotopic composition of TOC (d13C), the stable nitrogen isotopic composition of TON (d15N), and for the extraction of DNA for subsequent microbial community analyses based on sequencing 16S rRNA amplicons using 454 technology.<\/p><\/div>","@type":"rdf:HTML"}],"http:\/\/www.w3.org\/2000\/01\/rdf-schema#label":[{"@value":"Vent organic C and N","@type":"xsd:string"}],"http:\/\/ocean-data.org\/schema\/hasProcessingDescription":[{"@value":"
We have used a commercially available extraction kit for extracting the DNA. 16S rRNA amplicons for Bacteria and Archaea were generated using 454 sequence technology. Obtained sequences are currently being analyzed using the QIIME pipeline. The reads are being dereplicated, denoised, screened for chimeric sequences and taxonomically classified using the RDP and GreenGenes databases. Multivariate ordination techniques are being used to discriminate among samples with similar community structures. For TOC and TON analyses, samples are analyzed in triplicate using a Fisons 1108 Elemental Analyzer equipped with a Costech \"Zero Blank\" sample carousel. Effluent gases from the EA flow into a Finnigan-MAT Conflo-II interface attached to a DeltaPlus Isotope Ratio Mass Spectrometer. Standard materials (USGS-40 glutamic acid, IAEA-N1 ammonium sulfate, NBS-19 limestone, as well as known glycine and calcites) are used to determine the area response and calibrate the isotopic reference gas.\u00a0<\/p><\/div>","@type":"rdf:HTML"}],"http:\/\/purl.org\/dc\/terms\/identifier":[{"@value":"551070","@type":"xsd:int"}],"http:\/\/purl.org\/dc\/terms\/title":[{"@value":"Vent organic C and N"}],"http:\/\/purl.org\/dc\/terms\/date":[{"@value":"2015-02-18T09:41:04-05:00","@type":"xsd:dateTime"}],"http:\/\/purl.org\/dc\/terms\/created":[{"@value":"2015-02-18T09:41:04-05:00","@type":"xsd:dateTime"}],"http:\/\/purl.org\/dc\/terms\/modified":[{"@value":"2023-07-07T16:10:26-04:00","@type":"xsd:dateTime"}],"http:\/\/rdfs.org\/ns\/void#inDataset":[{"@id":"http:\/\/www.bco-dmo.org\/"}],"http:\/\/ocean-data.org\/schema\/namedGraph":[{"@value":"urn:bcodmo:dataset:551070","@type":"xsd:token"}],"http:\/\/ocean-data.org\/schema\/osprey_page":[{"@id":"https:\/\/www.bco-dmo.org\/dataset\/551070"}],"http:\/\/ocean-data.org\/schema\/identifier":[{"@value":"_:Identifier551070"}],"http:\/\/ocean-data.org\/schema\/datasetTitle":[{"@value":"Organic carbon and nitrogen in near-vent sediment samples in Paleochori Bay, Milos Island, Greece during 2012 (Hydrothermal Autotrophic Carbon Fixation project)","@language":"en-US"}],"http:\/\/ocean-data.org\/schema\/abstract":[{"@value":"","@language":"en-US"}],"http:\/\/purl.org\/dc\/terms\/rights":[{"@id":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"http:\/\/ocean-data.org\/schema\/deprecated":[{"@value":"false","@type":"xsd:boolean"}],"http:\/\/ocean-data.org\/schema\/spatialCoverage":[{"@value":"_:spatialCoverage551070"}],"http:\/\/purl.org\/dc\/terms\/bibliographicCitation":[{"@value":"Sievert, S. 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