http://lod.bco-dmo.org/id/dataset/551080 eng; USA utf8 dataset Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact 2015-02-18 ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data ISO 19115-2:2009(E) Temperature profiles along shallow-water hydothermal vents in Paleochori Bay, Milos Island, Greece during 2012 (Hydrothermal Autotrophic Carbon Fixation project) 2015-02-17 publication 2015-02-17 revision BCO-DMO Linked Data URI 2015-02-17 creation http://lod.bco-dmo.org/id/dataset/551080 Stefan M. Sievert Woods Hole Oceanographic Institution principalInvestigator Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. publisher Cite this dataset as: Sievert, S. M. (2015) Temperature profiles along shallow-water hydothermal vents in Paleochori Bay, Milos Island, Greece during 2012 (Hydrothermal Autotrophic Carbon Fixation project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 2015-02-17) Version Date 2015-02-17 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/551080 [access date] Temperature profile along transect at shallow-water hydothermal vent site in Paleochori Bay, Milos Island, Greece Dataset Description: &lt;p&gt;This dataset describes the temperature profiles taken by Scuba diving at a shallow-water hydrothermal vent site in Paleochori Bay, Milos Island, Greece.&lt;/p&gt; Methods and Sampling: Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1124272 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1124272 completed Stefan M. Sievert Woods Hole Oceanographic Institution 508 289 2305 Biology Department Watson Bldg. MS #52 Woods Hole MA 02543 USA ssievert@whoi.edu pointOfContact asNeeded Dataset Version: 2015-02-17 Unknown location lat lon site depth distance temp Digital thermometer theme None, User defined region latitude longitude site depth No BCO-DMO term water temperature at measurement depth featureType BCO-DMO Standard Parameters Water Temperature Sensor instrument BCO-DMO Standard Instruments Milos_vents_2012 service Deployment Activity Milos Island, Greece place Locations otherRestrictions otherRestrictions Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use. Center for Dark Energy Biosphere Investigations http://www.darkenergybiosphere.org Center for Dark Energy Biosphere Investigations The mission of the Center for Dark Energy Biosphere Investigations (C-DEBI) is to explore life beneath the seafloor and make transformative discoveries that advance science, benefit society, and inspire people of all ages and origins. C-DEBI provides a framework for a large, multi-disciplinary group of scientists to pursue fundamental questions about life deep in the sub-surface environment of Earth. The fundamental science questions of C-DEBI involve exploration and discovery, uncovering the processes that constrain the sub-surface biosphere below the oceans, and implications to the Earth system. What type of life exists in this deep biosphere, how much, and how is it distributed and dispersed? What are the physical-chemical conditions that promote or limit life? What are the important oxidation-reduction processes and are they unique or important to humankind? How does this biosphere influence global energy and material cycles, particularly the carbon cycle? Finally, can we discern how such life evolved in geological settings beneath the ocean floor, and how this might relate to ideas about the origin of life on our planet? C-DEBI's scientific goals are pursued with a combination of approaches: (1) coordinate, integrate, support, and extend the research associated with four major programs—Juan de Fuca Ridge flank (JdF), South Pacific Gyre (SPG), North Pond (NP), and Dorado Outcrop (DO)—and other field sites; (2) make substantial investments of resources to support field, laboratory, analytical, and modeling studies of the deep subseafloor ecosystems; (3) facilitate and encourage synthesis and thematic understanding of submarine microbiological processes, through funding of scientific and technical activities, coordination and hosting of meetings and workshops, and support of (mostly junior) researchers and graduate students; and (4) entrain, educate, inspire, and mentor an interdisciplinary community of researchers and educators, with an emphasis on undergraduate and graduate students and early-career scientists. Note: Katrina Edwards was a former PI of C-DEBI; James Cowen is a former co-PI. Data Management: C-DEBI is committed to ensuring all the data generated are publically available and deposited in a data repository for long-term storage as stated in their Data Management Plan (PDF) and in compliance with the NSF Ocean Sciences Sample and Data Policy. The data types and products resulting from C-DEBI-supported research include a wide variety of geophysical, geological, geochemical, and biological information, in addition to education and outreach materials, technical documents, and samples. All data and information generated by C-DEBI-supported research projects are required to be made publically available either following publication of research results or within two (2) years of data generation. To ensure preservation and dissemination of the diverse data-types generated, C-DEBI researchers are working with BCO-DMO Data Managers make data publicly available online. The partnership with BCO-DMO helps ensure that the C-DEBI data are discoverable and available for reuse. Some C-DEBI data is better served by specialized repositories (NCBI's GenBank for sequence data, for example) and, in those cases, BCO-DMO provides dataset documentation (metadata) that includes links to those external repositories. C-DEBI largerWorkCitation program Autotrophic carbon fixation at a shallow-water hydrothermal system: Constraining microbial activity, isotopic and geochemical regimes https://osprey.bco-dmo.org/project/473892 Autotrophic carbon fixation at a shallow-water hydrothermal system: Constraining microbial activity, isotopic and geochemical regimes <p>In this project we studied the shallow-water hydrothermal vent sites at Milos Island (Greece) to better understand the extent of autotrophic carbon fixation and its chemical and isotopic signature along environmental (redox/thermal) gradients. This was a 12-day long expedition (May 18 to 30, 2012) to sample vent fluids, gases and retrieve sediment cores at Paleochori Bay by using SCUBA diving at 8-10 m depth. In addition to the submarine vent sites, two subaerial locations of venting were identified at 36o 40' 28"N - 24o 31' 14" E and 36o 40' 25" N - 24o 30' 44" E. Both the subaerial and submarine sites are located on the same fracture zone that likely controls the hydrothermal circulation of evolved meteoritic water and seawater within the magmatic zone of Milos Island. To this end, the geochemistry of the fluids and gases emitted from subaerial sites provide important information towards identifying the linkage between the subaerial and submarine magmatic activity and provide insights on the metabolic functions (e.g. H2 oxidation, Fe(III) reduction, C and S cycling) of the subsurface microbial community. </p> <p>Abstract:<br /> Currently, there is only limited information on the identity and activity of the microorganisms carrying out CO2-fixation in situ, despite the fact that these organisms form the basis of their respective ecosystems. Representatives that are able to grow autotrophically are known to exist in almost all major groups of prokaryotes, and these organisms play essential roles in ecosystems by providing a continuous supply of organic carbon for heterotrophs. Microorganisms present in extreme environments utilize CO2- fixation pathways other than the Calvin-Benson-Bassham (CBB) cycle. At present, five alternative autotrophic CO2 fixation pathways are known. Different carbon fixation pathways result in distinct isotopic signatures of the produced biomass due to the isotopic discrimination between light (12C) and heavy (13C) carbon by the carboxylating enzymes. Thus, inferences about the carbon fixation pathway predominantly utilized by the microbial community can also be made based on the stable carbon isotopic composition of the organic matter, in extant systems as well as in the geological record. However, at present little is known about the systematics and extents of fractionation during carbon fixation by prokaryotic organisms, and to our knowledge no studies exist that have systematically studied the relationship between the operation of different carbon fixation pathways and how this is reflected in the stable carbon isotopic composition in a natural system. This is a 2-year interdisciplinary, international research program that employs a powerful combination of cutting-edge research tools aiming to improve our understanding of autotrophic carbon fixation and its chemical and isotopic signature along environmental gradients in a natural hydrothermal system. The following hypotheses are addressed:</p> <p>1. The diversity of microorganisms present along a thermal and redox gradient, and rates of CO2 fixation, will reflect adaptation to in situ temperatures and geochemical conditions</p> <p>2. Microorganisms utilizing the CBB cycle for autotrophic CO2-fixation will represent a smaller percentage of the chemolithoautotrophic community at higher temperatures, where microorganisms utilizing alternative CO2-fixation pathways dominate</p> <p>3. Isotopic values of biomass and specific biomarker molecules will vary along a thermal and redox gradient from zones characterized by a higher hydrothermal fluid flux and thus higher temperatures to the surrounding, cooler areas, corresponding to the physiology of the microorganisms utilizing different pathways for carbon fixation</p> <p>The PIs will use a multidisciplinary approach to delineate the relative contribution of the different carbon fixation pathways along an environmental gradient by combining metagenomic analyses coupled with: 1) an assessment of the frequency and the expression of specific key genes involved in carbon fixation, and 2) with the measurement of carbon fixation rates. These data will be integrated with the determination of stable C isotopic composition of biomass, DIC, and specific hydrocarbons/lipids. Due to its easy accessibility, well-established environmental gradients, and extensive background information, the shallow-water vents off Milos (Greece) will be used as a natural laboratory to perform these studies.</p> <p>Intellectual Merit. The data generated in this study will allow constraints on the relationship between autotrophic carbon fixation and the resulting isotopic signatures of biomass and specific biomarker molecules (e.g. CH4, C2+ alkanes, lipids) in a natural system. This has implications for assessing the importance of carbon fixation in extant ecosystems, and it will also provide a tool to improve the interpretation of isotopic values in the geological record.</p> Hydrothermal Autotrophic Carbon Fixation largerWorkCitation project eng; USA oceans Milos Island, Greece 2015-02-17 Shallow-water hydrothermal vents, Paleochori Bay, Milos Island, Greece 0 BCO-DMO catalogue of parameters from Temperature profiles along shallow-water hydothermal vents in Paleochori Bay, Milos Island, Greece during 2012 (Hydrothermal Autotrophic Carbon Fixation project) Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact http://lod.bco-dmo.org/id/dataset-parameter/551090.rdf Name: location Units: unitless Description: <p>sampling location</p> http://lod.bco-dmo.org/id/dataset-parameter/551091.rdf Name: lat Units: decimal degrees Description: <p>latitude; north is positive</p> http://lod.bco-dmo.org/id/dataset-parameter/551092.rdf Name: lon Units: decimal degrees Description: <p>longitude; east is positive</p> http://lod.bco-dmo.org/id/dataset-parameter/551093.rdf Name: site Units: unitless Description: <p>sampling site number</p> http://lod.bco-dmo.org/id/dataset-parameter/551094.rdf Name: depth Units: cm Description: <p>depth of temperature</p> http://lod.bco-dmo.org/id/dataset-parameter/551095.rdf Name: distance Units: cm Description: <p>distance along transect</p> http://lod.bco-dmo.org/id/dataset-parameter/551096.rdf Name: temp Units: degrees Celsius Description: <p>temperature</p> GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact 2744 https://datadocs.bco-dmo.org/file/M77XqRzfkKRMRP/temp_profile.csv temp_profile.csv Primary data file for dataset ID 551080 download https://osprey.bco-dmo.org/dataset/551080/data/download download onLine dataset &lt;p&gt;Temperature measurements were conducted in situ by Scuba diving at different locations along the transect from the hottest part of the venting area towards the unaffected, colder sediments, i.e., at 0 cm, 50 cm, 100 cm, 150 cm, 200 cm, and 300 cm, at 5 cm intervals to a maximum sediment depth of 30 cm with a digital thermometer in a custom underwater housing.&lt;/p&gt; Specified by the Principal Investigator(s) asNeeded 7.x-1.1 Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact Digital thermometer Digital thermometer PI Supplied Instrument Name: Digital thermometer PI Supplied Instrument Description:Digital thermometer in custom underwater housing Instrument Name: Water Temperature Sensor Instrument Short Name:Water Temp Sensor Instrument Description: General term for an instrument that measures the temperature of the water with which it is in contact (thermometer). Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/134/ Deployment: Milos_vents_2012 Milos_vents_2012 shoreside Milos_vents island Milos_vents_2012 Dionysis Foustoukos Carnegie Institution for Science shoreside Milos_vents island