1b:I[42426,["/_next/static/immutable/chunks/1xctgzqre-wsy.js","/_next/static/immutable/chunks/06_-wz6pdtp2b.js","/_next/static/immutable/chunks/30e52g49hg40p.js","/_next/static/immutable/chunks/02yq6tul2z-oh.js","/_next/static/immutable/chunks/10bx_ec2vj7n_.js","/_next/static/immutable/chunks/33bfn03fo8xnt.js","/_next/static/immutable/chunks/0tfjf78scu61b.js","/_next/static/immutable/chunks/31xnvej4n1bi9.js"],"default"] 1c:Tec7,Intellectual Merit: Bacteria that use both dissolved organic material (DOM) and light, i.e. photoheterotrophs, would fundamentally change views of how energy and material are processed in the oceans. However, it is still not clear if these microbes have unique roles in the oceans because standard experiments have not been successful in consistently demonstrating positive effects of light on growth and respiration of presumed photoheterotrophs. It is known that these microbes are abundant, with one type (those containing proteorhodopsin) alone constituting 50% or more of all microbes in the oceans. But why these microbes are so abundant is unknown as the ecological advantages of photoheterotrophy remain obscure. The PIs will use a new approach and novel experiments to examine how light affects photoheterotrophs and to explore the contribution of these microbes to DOM fluxes. Their work is testing the following hypothesis: The biogeochemical role of photoheterotrophs is to use low energy-yielding DOM components such as products of photochemical reactions. The reactions involve chromophoric DOM (CDOM) which is a large and dynamic part of the carbon cycle especially in coastal oceans. They have hypothesized that the light energy gained by photoheterotrophs would enable these microbes to benefit from using photochemically-produced compounds which alone do not yield much energy. This hypothesis is supported by lab experiments showing that proteorhodopsin-generated energy becomes important only when respiration is inhibited and cells are limited by energy. Other lab experiments demonstrated that anaplerotic fixation of CO2 by PR-containing bacteria is stimulated by light. This fixation is needed for growth on C1-C4 compounds, including many produced by photochemical reactions. The PIs are testing this hypothesis with experiments in the Delaware estuary where CDOM varies greatly spatially and seasonally. They are examining the effect of light (PAR) on the uptake and respiration of photochemically-produced low molecular weight (LMW) organic compounds and on gene expression (mRNA) of photoheterotrophs. The focus is on CO, pyruvate, acetaldehyde, and glyoxal; together these compounds constitute a large fraction of the photochemical-byproducts in seawater. Glycolate is also being examined because of its importance in phytoplankton excretion and because of its similarity to organic acids produced by photochemical reactions. Uptake of these compounds is estimated with 14C- tracers and HPLC measurements of concentrations. Rates are then compared with the abundance and mRNA levels of proteorhodopsin and pufM found in aerobic anoxygenic phototrophic bacteria as measured by QPCR assays. The PIs are also examining how light and the photochemically-produced LMW organic compounds affect bacterial respiration and growth efficiency. They are examining the relationships among anaplerotic CO2 fixation, uptake of photochemical byproducts, and photoheterotroph abundance and activity along transects of the Delaware estuary and during diel studies. The proposed work is being conducted by a team consisting of microbial oceanographers (Kirchman and Cottrell) and a marine biogeochemist (Kieber) with expertise in photoheterotrophs and photochemical reactions, respectively. Broader Impacts: This interdisciplinary project is supporting graduate students and also involves undergraduates in summer research projects. Results will be incorporated into web sites and used in courses taught by Kirchman and Kieber. The Kirchman lab is featured in lab tours open to the public and in Coast Day, an annual open house that attracts about 10,000 visitors. Kieber mentors undergraduates and coordinated a program for economically disadvantaged high school students.6:["$","$L1b",null,{"dataset":{"_id":"551239","description":"

Nutrients, chlorophyll-a, and light attenuation in the Delaware estuary from the R/V Hugh R. Sharp HRS110805DK, HRS111107DK, HRS120809DK, HRS121112DK, HRS1313, HRS1324 in 2011 - 2013 (PAPI: Photochemistry and Photoheterotroph Interactions project)

\n","problem":"","acquisition":"

Nutrient concentrations were measured by standard wet chemical methods using a SEAL Analytical AA3 Continuous Segmented Flow Analyzer.

Samples for chlorophyll a concentrations were collected by filtering 100 ml of estuarine water through Whatman GF/F filters and stored at -20 oC until analysis. To estimate concentrations, the filters were placed into 90% acetone and 40% dimethyl sulfoxide (DMSO) and then the fluorescence in the extract was measured with a Turner Designs 10-AU fluorometer.

The attenuation coefficient was estimated by measuring photosynthetically active radiance with a Biospherical PNF-210 radiometer over a depth profile. In nearly all cases, the downcast and upcast profiles of radiance were indistinguishable and all data were used. When differences between the down and upcasts were apparent, only the downcast data were used.

\n","processing":"

Except for converting raw spectrometric or fluorometric readings to concentrations, the concentration data were not processed.

Radiance values at very shallow or very deep depths were excluded from the analysis to calculate the attenuation coefficient when these values were clearly not along the ln(radiance) vs. depth line.

BCO-DMO Processing:

- added conventional header with dataset name, PI name, version date, reference information
\n- renamed parameters to BCO-DMO standard
\n- reformated date from m/d/yyyy to yyyy-mm-dd
\n- replaced blank cells with nd; changed NA and ND to nd
\n- replaced blanks and / with underscores
\n- changed format of latititude and longitude to decimal degrees
\n- matched cruise names to R2R standard names
\n- revised 3 lat/lon positions - new version (2015-03-11) replaces 2015-01-19
\n- revised 2 lat positions - new version (2020-10-01)

\n","bcodmoProcessing":"","title":"Nutrients, chlorophyll-a, and light attenuation in the Delaware estuary from the R/V Hugh R. Sharp HRS110805DK, HRS111107DK, HRS120809DK, HRS121112DK, HRS1313, HRS1324 in 2011 - 2013 (PAPI: Photochemistry and Photoheterotroph Interactions project)","nickname":"Delaware estuary nutrients","dataUrl":"","currentState":"Final no updates expected","currentStateId":"7","validated":true,"restricted":false,"version":"2","version_date":"2020-10-01T04:00:00.000Z","abstract":"Nutrients, chlorophyll-a, and light attenuation in the Delaware estuary from the R/V Hugh R. Sharp HRS110805DK, HRS111107DK, HRS120809DK, HRS121112DK, HRS1313, HRS1324 in 2011 - 2013 (PAPI: Photochemistry and Photoheterotroph Interactions project)","citation":"Kieber, D. J., Kirchman, D. L. (2020) Nutrients, chlorophyll-a, and light attenuation in the Delaware estuary from the R/V Hugh R. Sharp HRS110805DK, HRS111107DK, HRS120809DK, HRS121112DK, HRS1313, HRS1324 in 2011 - 2013 (PAPI: Photochemistry and Photoheterotroph Interactions project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 2) Version Date 2020-10-01 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.551239.2 [access date]","citationParts":{"identifier":"doi:10.26008/1912/bco-dmo.551239.2","versionDate":"2020-10-01","versionId":"2","year":"2020","title":"Nutrients, chlorophyll-a, and light attenuation in the Delaware estuary from the R/V Hugh R. Sharp HRS110805DK, HRS111107DK, HRS120809DK, HRS121112DK, HRS1313, HRS1324 in 2011 - 2013 (PAPI: Photochemistry and Photoheterotroph Interactions project)","authors":"Kieber, D. J., Kirchman, D. L.","publisher":"Biological and Chemical Oceanography Data Management Office (BCO-DMO)."},"doi":"10.26008/1912/bco-dmo.551239.2","release_date":null,"field_dataset_location":"","temporal_extent":{"start":"2011-08-05T04:00:00.000Z","end":"2013-11-21T05:00:00.000Z"},"temporal":{"resolution":"time:unitDay","type":"time:Interval","start":{"value":"1312516800","label":"2011-08-05"},"end":{"value":"1385010000","label":"2013-11-21"}},"spatial_extent":{"shape":"polygon","center":{"latitude":20.2516335,"longitude":-38.4155335},"wkt":"POLYGON ((-75.5825 0.646267, -1.248567 0.646267, -1.248567 39.857, -75.5825 39.857, -75.5825 0.646267))","north":39.857,"east":-1.248567,"south":0.646267,"west":-75.5825},"dataset_type":["Cruise Results"],"keywords":["oceans"],"data_files":[{"id":"900455","title":"PAPI_nuts_v2.csv","url":"https://datadocs.bco-dmo.org/dataset/551239/file/N77GrVAf9p8rN7/PAPI_nuts_v2.csv","showTableUrl":"https://www.bco-dmo.org/dataset/551239/data/view/900455","description":"Primary data file for dataset ID 551239","filename":"PAPI_nuts_v2.csv","bytesize":21061,"checksum":{"value":"681f2e6e14efc2c76a73287714268a30","Algorithm":{"_id":"190","name":"MD5"}},"mimetype":"text/csv","fileType":{"_id":"192","name":"Comma Separated Values (.csv)"},"isErddap":"","date":"","isPublicDuringEmbargo":false,"primaryDataFile":true,"showTable":true,"restricted":false}],"supplemental_files":[],"roles":[{"role":{"_id":"138","name":"Principal Investigator"},"performedBy":{"_id":"544597","name":"David J. Kieber"},"performedAtOrganization":{"_id":"516269","name":"State University of New York College of Environmental Science and Forestry"}},{"role":{"_id":"138","name":"Principal Investigator"},"performedBy":{"_id":"50584","name":"David L. Kirchman"},"performedAtOrganization":{"_id":"70","name":"University of Delaware"}},{"role":{"_id":"133","name":"Contact"},"performedBy":{"_id":"50584","name":"David L. Kirchman"},"performedAtOrganization":{"_id":"70","name":"University of Delaware"}},{"role":{"_id":"145","name":"BCO-DMO Data Manager"},"performedBy":{"_id":"50396","name":"Nancy Copley"},"performedAtOrganization":{"_id":"191","name":"Woods Hole Oceanographic Institution"}}],"deployments":[{"_id":"551245","name":"HRS110805DK","synonyms":["PAPI-1"],"dates":{"start":"2011-08-05T00:00:00.000Z","end":"2011-08-09T00:00:00.000Z"},"platform":{"_id":"54151","name":"R/V Hugh R. Sharp"},"chiefScientists":[{"id":"50584","name":"David L. Kirchman","role":"Chief Scientist"}]},{"_id":"551247","name":"HRS111107DK","synonyms":["PAPI-2"],"dates":{"start":"2011-11-07T00:00:00.000Z","end":"2011-11-11T00:00:00.000Z"},"platform":{"_id":"54151","name":"R/V Hugh R. Sharp"},"chiefScientists":[{"id":"50584","name":"David L. Kirchman","role":"Chief Scientist"}]},{"_id":"551249","name":"HRS120809DK","synonyms":["PAPI-3"],"dates":{"start":"2012-08-08T00:00:00.000Z","end":"2012-08-13T00:00:00.000Z"},"platform":{"_id":"54151","name":"R/V Hugh R. Sharp"},"chiefScientists":[{"id":"50584","name":"David L. Kirchman","role":"Chief Scientist"}]},{"_id":"551250","name":"HRS121112DK","synonyms":["PAPI-4"],"dates":{"start":"2012-11-12T00:00:00.000Z","end":"2012-11-16T00:00:00.000Z"},"platform":{"_id":"54151","name":"R/V Hugh R. Sharp"},"chiefScientists":[{"id":"50584","name":"David L. Kirchman","role":"Chief Scientist"}]},{"_id":"551252","name":"HRS1313","synonyms":["PAPI-5","HRS1313DK"],"dates":{"start":"2013-08-03T00:00:00.000Z","end":"2013-08-07T00:00:00.000Z"},"platform":{"_id":"54151","name":"R/V Hugh R. Sharp"},"chiefScientists":[{"id":"50584","name":"David L. Kirchman","role":"Chief Scientist"}]},{"_id":"551257","name":"HRS1324","synonyms":["PAPI-6","HRS1324DK"],"dates":{"start":"2013-11-17T00:00:00.000Z","end":"2013-11-22T00:00:00.000Z"},"platform":{"_id":"54151","name":"R/V Hugh R. Sharp"},"chiefScientists":[{"id":"50584","name":"David L. Kirchman","role":"Chief Scientist"}]}],"funding":[{"_id":"544593","name":"OCE-1029569","fundingSource":{"_id":"355","name":"NSF Division of Ocean Sciences"},"url":"https://www.nsf.gov/awardsearch/show-award?AWD_ID=1029569"},{"_id":"544603","name":"OCE-1030306","fundingSource":{"_id":"355","name":"NSF Division of Ocean Sciences"},"url":"https://www.nsf.gov/awardsearch/show-award?AWD_ID=1030306"}],"instruments":[{"_id":"551266","name":"Fluorometer","description":"Turner Designs 10-AU fluorometer","type_id":"484","type_name":"Fluorometer","type_desc":"A fluorometer or fluorimeter is a device used to measure parameters of fluorescence: its intensity and wavelength distribution of emission spectrum after excitation by a certain spectrum of light. The instrument is designed to measure the amount of stimulated electromagnetic radiation produced by pulses of electromagnetic radiation emitted into a water sample or in situ.","type_acronym":"Fluorometer"},{"_id":"551265","name":"","description":"SEAL Analytical AA3 Continuous Segmented Flow Analyzer","type_id":"558","type_name":"Nutrient Autoanalyzer","type_desc":"Nutrient Autoanalyzer is a generic term used when specific type, make and model were not specified. In general, a Nutrient Autoanalyzer is an automated flow-thru system for doing nutrient analysis (nitrate, ammonium, orthophosphate, and silicate) on seawater samples.","type_acronym":"Nutrient Autoanalyzer"}],"parameters":[{"_id":"551267","name":"cruise_id","format":"","frictionlessType":"","description":"

cruise identification

","units":{"_id":"","name":"unitless"},"type":{"_id":"1102","name":"cruise_id","description":"

cruise designation; name

"}},{"_id":"551268","name":"cruise_name","format":"","frictionlessType":"","description":"

project assigned cruise name

","units":{"_id":"","name":"unitless"},"type":{"_id":"1929","name":"Cruise Name","description":"

The cruise_name is a local name chosen by project investigators for a research expedition on a vessel, as opposed to the formal/official cruise ID asserted by the vessel operator.

"}},{"_id":"551269","name":"date","format":"","frictionlessType":"","description":"

local sampling date

","units":{"_id":"","name":"yyyy-mm-dd"},"type":{"_id":"1070","name":"date","description":"

date; generally reported in GMT as YYYYMMDD (year; month; day); also as MMDD (month; day); EqPac dates are local Hawaii time. ISO_Date format is YYYY-MM-DD (http://www.iso.org/iso/home/standards/iso8601.htm)

"}},{"_id":"551270","name":"time_local","format":"","frictionlessType":"","description":"

local time

","units":{"_id":"","name":"HH:MM"},"type":{"_id":"1007","name":"time_local","description":"

time of day, local time, using 2400 clock format

"}},{"_id":"551271","name":"lat","format":"","frictionlessType":"","description":"

latitude; north is positive

","units":{"_id":"","name":"decimal degrees"},"type":{"_id":"730","name":"latitude","description":"

latitude, in decimal degrees, North is positive, negative denotes South; Reported in some datasets as degrees, minutes

"}},{"_id":"551272","name":"lon","format":"","frictionlessType":"","description":"

longitiude; east is positive

","units":{"_id":"","name":"decimal degrees"},"type":{"_id":"731","name":"longitude","description":"

longitude, in decimal degrees, East is positive, negative denotes West; Reported in some datsets as degrees, minutes

"}},{"_id":"551273","name":"station","format":"","frictionlessType":"","description":"

station

","units":{"_id":"","name":"unitless"},"type":{"_id":"987","name":"station","description":"

Station identifier; a unique number or alphanumeric string designating a general geographic location at which one or more sampling activities may occur.

"}},{"_id":"551274","name":"cast","format":"","frictionlessType":"","description":"

cast number

","units":{"_id":"","name":"unitless"},"type":{"_id":"761","name":"cast","description":"

cast or profile number

"}},{"_id":"551275","name":"light_atten","format":"","frictionlessType":"","description":"

light attenuation

","units":{"_id":"","name":"per meter"},"type":{"_id":"747","name":"beam_c","description":"

attenuation (loss of light) of a narrow, well collimated beam of light; beam attenuation due to particles; the particulate beam attenuation coefficient (cp); beam attenuation coefficient, in units 1/meter

"}},{"_id":"551276","name":"atten_err","format":"","frictionlessType":"","description":"

light attenuation error; calculated by linear regression analysis of ln(irradiance) vs. depth

","units":{"_id":"","name":"per meter"},"type":{"_id":"747","name":"beam_c","description":"

"}},{"_id":"551277","name":"chl_a","format":"","frictionlessType":"","description":"

chlorophyll-a concentration

","units":{"_id":"","name":"microgram/liter"},"type":{"_id":"766","name":"chlorophyll a","description":"

Total chlorophyll a pigment. See dataset for units of measurement; often reported in: milligrams per cubic meter (mg/m3); micrograms/liter (ug/L); nanograms/liter (ng/L).

CAUTION Within JGOFS MERGED water column products: this parameter is derived from HPLC methodology and the units are nanograms/liter; however; this parameter name is also used for a variety of water and nonwater column sampling; derived via the fluorometric method; units will also vary.

"}},{"_id":"551278","name":"chl_a_sd","format":"","frictionlessType":"","description":"

chlorophyll-a concentration standard deviation

","units":{"_id":"","name":"microgram/liter"},"type":{"_id":"1974","name":"standard deviation","description":"

Generic standard deviation value, taking on the units of the parameter that it is tied to.

"}},{"_id":"551279","name":"NO3","format":"","frictionlessType":"","description":"

nitrate concentration

","units":{"_id":"","name":"micromoles/liter"},"type":{"_id":"1194","name":"NO3","description":"

Nitrate concentration in the water column

"}},{"_id":"551280","name":"NO3_sd","format":"","frictionlessType":"","description":"

nitrate concentration standard deviation

","units":{"_id":"","name":"micromoles/liter"},"type":{"_id":"1974","name":"standard deviation","description":"

Generic standard deviation value, taking on the units of the parameter that it is tied to.

"}},{"_id":"551281","name":"NH4","format":"","frictionlessType":"","description":"

ammonium concentration

","units":{"_id":"","name":"micromoles/liter"},"type":{"_id":"901","name":"Ammonium","description":"

Ammonium and ammonia concentration parameters in any body of fresh or salt water.

"}},{"_id":"551282","name":"NH4_sd","format":"","frictionlessType":"","description":"

ammonium concentration standard deviation

","units":{"_id":"","name":"micromoles/liter"},"type":{"_id":"1974","name":"standard deviation","description":"

Generic standard deviation value, taking on the units of the parameter that it is tied to.

"}},{"_id":"551283","name":"PO4","format":"","frictionlessType":"","description":"

phosphate concentration

","units":{"_id":"","name":"micromoles/liter"},"type":{"_id":"937","name":"PO4","description":"

Orthophosphate (phosphate, reactive phosphorus), Various units.

"}},{"_id":"551284","name":"PO4_sd","format":"","frictionlessType":"","description":"

phosphate concentration standard deviation

","units":{"_id":"","name":"micromoles/liter"},"type":{"_id":"1974","name":"standard deviation","description":"

Generic standard deviation value, taking on the units of the parameter that it is tied to.

"}},{"_id":"551285","name":"SiO4","format":"","frictionlessType":"","description":"

silicate concentration

","units":{"_id":"","name":"micromoles/liter"},"type":{"_id":"1209","name":"SiO4","description":"

Silicate; reactive silica. Also referred to as dissolved silicate, dissolved silica.

"}},{"_id":"551286","name":"SiO4_sd","format":"","frictionlessType":"","description":"

silicate concentration standard deviation

","units":{"_id":"","name":"micromoles/liter"},"type":{"_id":"1974","name":"standard deviation","description":"

Generic standard deviation value, taking on the units of the parameter that it is tied to.

"}},{"_id":"825843","name":"comment","format":"","frictionlessType":"","description":"

sampling comments

","units":{"_id":"","name":"unitless"},"type":{"_id":"778","name":"comment","description":"

free text comments, may only have meaning to submitting PI

"}}],"related_publications":[],"related_datasets":[],"services":[{"grouping":"","items":[{"name":"View Data","description":"An in-browser view of the primary tabular data file","url":"https://www.bco-dmo.org/dataset/551239/data/view","contentType":"text/html","actionType":"view"}]},{"grouping":"Metadata","items":[{"name":"HTML","description":"A simple HTML page of the dataset metadata","url":"https://www.bco-dmo.org/dataset/551239/description","contentType":"text/html","actionType":"view"},{"name":"PDF","description":"A PDF of the HTML dataset metadata","url":"https://www.bco-dmo.org/dataset/551239/Dataset_description.pdf","contentType":"application/pdf","actionType":"download"},{"name":"ISO 19115-2 (NOAA Profile)","description":"","url":"https://www.bco-dmo.org/dataset/551239/iso","contentType":"application/xml","actionType":"download"}]}],"programs":[],"projects":[{"_id":"544594","name":"Activity and abundance of photoheterotrophs fueled by photochemically-produced substrates","acronym":"PAPI: Photochemistry and Photoheterotroph Interactions","description":"$1c"}],"archives":[{"id_value":"10.26008/1912/bco-dmo.551239.2","url":"https://doi.org/10.26008/1912/bco-dmo.551239.2","date_assigned":"2020-10-01","version_date":"2020-10-01","archiver":"Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)","archiver_id":"272"}],"license":{"name":"Creative Commons Attribution 4.0","id":"CC-BY-4.0","uri":"https://creativecommons.org/licenses/by/4.0/","spdxUri":"https://spdx.org/licenses/CC-BY-4.0"},"metrics":{"views":{"count":252},"downloads":{"count":768}}},"fileId":"900455"}]