Zooplankton net haul metadata and specimen removal records collected from the R/V Gunnerus in the Tronheimsfjord, Norway during 2012 (Calanus Diapause project)

Website: https://www.bco-dmo.org/dataset/551905
Version: 2015-02-18

» Identification of Diagnostic Markers of Diapause Preparation in the Copepod Calanus finmarchicus (Calanus_Diapause)
Baumgartner, MarkWoods Hole Oceanographic Institution (WHOI)Principal Investigator
Tarrant, Ann M.Woods Hole Oceanographic Institution (WHOI)Co-Principal Investigator
Copley, NancyWoods Hole Oceanographic Institution (WHOI BCO-DMO)BCO-DMO Data Manager

Dataset Description

This dataset consists of metadata associated with zooplankton net tows taken in Trondheimsfjord, Norway in spring 2012. A list of the specimens removed from the sample are also provided here.

Methods & Sampling

Zooplankton samples were collected in Trondheimsfjord near Trondheim, Norway from 3 May to 11 June 2012 using a Sea-Gear 75-cm diameter ring net equipped with 150 micron mesh net and a cod end.  Oblique tows were conducted over the upper ~50 m for nearly all tows.  A close-open-close mechanism was used to collect zooplankton samples with the same net between roughly 250 and 350 m on three occasions.  The volume filtered by the net was estimated using a General Oceanics flowmeter suspended in the center of the net mouth, and sample depths were measured using a SeaBird SBE39 depth-temperature recorder.  Zooplankton samples were held on ice during transport back to the NTNU/SINTEF laboratory in Trondheim.  Samples were live sorted to remove some late-stage Calanus finmarchicus, and then preserved in 5% buffered formalin.  A small aliquot (subsample) of the bulk sample was removed and all taxa contained therein were enumerated.  Species- or taxon-specific abundance of zooplankton was estimated from the raw counts, the subsample volume, sample volume, and the volume filtered by the net.

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Data Files

(Comma Separated Values (.csv), 6.45 KB)
Primary data file for dataset ID 551905

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stationsampling location unitless
cruise_namecruise name assigned by PI unitless
yearsampling year yyyy
haulplankton tow number unitless
datesampling date yyyy-mm-dd
lat_startlatitude at start of haul; north is positive decimal degrees
lon_startlongitude at start of haul; east is positive decimal degrees
lat_endlatitude at end of haul; north is positive decimal degrees
lon_endlongitude at end of haul; east is positive decimal degrees
time_startlocal time at start of haul HH:MM:SS
time_endlocal time at end of haul HH:MM:SS
wire_outmeters of wire out meters
depth_wdepth of water meters
gear_typetype of sampling gear: ring = ring net unitless
meshplankton net mesh size microns
net_diamnet diameter meters
haul_typetype of haul: vertical or oblique unitless
depth_startdepth at start of sample collection meters
depth_enddepth at end of sample collection meters
flow_serial_numflow meter serial number unitless
flow_count_startinitial flow count reading unitless
flow_count_endfinal flow count reading unitless
flow_revolutionsnumber of flow count revolutions revolutions
flow_cal_constantflow meter calibration constant meters/revolution
volume_haulvolume of water filtered during haul cubic meters
taxa_removed_1taxa removed from sample (1) unitless
num_removed_1Number removed from sample (1) specimens
taxa_removed_2taxa removed from sample (2) unitless
num_removed_2Number removed from sample (2) specimens
taxa_removed_3taxa removed from sample (3) unitless
num_removed_3Number removed from sample (3) specimens
vol_subsamplevolume of subsample milliliters
vol_samplesample volume milliliters
processed_flagflag designating sample processed: 1 = yes unitless
ISO_DateTime_Local_startLocal date and time in ISO format YYYY-MM-DDTHH:MM:SS.xx

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Dataset-specific Instrument Name
Ring Net
Generic Instrument Name
Ring Net
Dataset-specific Description
Sea-Gear 75-cm diameter ring net equipped with 150 micron mesh net and a cod end
Generic Instrument Description
A Ring Net is a generic plankton net, made by attaching a net of any mesh size to a metal ring of any diameter. There are 1 meter, .75 meter, .25 meter and .5 meter nets that are used regularly. The most common zooplankton ring net is 1 meter in diameter and of mesh size .333mm, also known as a 'meter net' (see Meter Net).

Dataset-specific Instrument Name
Temperature and depth recorder
Generic Instrument Name
Water Temperature Sensor
Dataset-specific Description
SeaBird SBE39 depth-temperature recorder
Generic Instrument Description
General term for an instrument that measures the temperature of the water with which it is in contact (thermometer).

Dataset-specific Instrument Name
Flow Meter
Generic Instrument Name
Flow Meter
Dataset-specific Description
General Oceanics flowmeter
Generic Instrument Description
General term for a sensor that quantifies the rate at which fluids (e.g. water or air) pass through sensor packages, instruments, or sampling devices. A flow meter may be mechanical, optical, electromagnetic, etc.

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R/V Gunnerus
Start Date
End Date
Zooplankton sampling on the RV Gunnerus (102') and RV Calanus (~22') out of the Norwegian University of Science and Technology (NTNU). Sampling by day trips in Trondheimsfjord.

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Project Information

Identification of Diagnostic Markers of Diapause Preparation in the Copepod Calanus finmarchicus (Calanus_Diapause)

Coverage: Trondheimfjord (near Trondheim, Norway)

Extracted from the NSF award abstract:

Oceanic copepods in the family Calanidae, such as Calanus finmarchicus, are among the most abundant animals on the planet, and they serve a key role in marine food webs by transferring energy from phytoplankton to higher trophic levels. The life history of these copepods has been well-studied, but fundamental questions remain about the regulation of an important period of dormancy called diapause. In the last juvenile stage of development, C. finmarchicus either proceed to the terminal molt (i.e., molt into adults) or vertically migrate to depth and initiate diapause. This divergence in developmental pathways has critical implications for C. finmarchicus population dynamics, but is difficult to study experimentally because C. finmarchicus, like all other copepods in the family Calanidae, do not reliably enter diapause in the laboratory. In addition, most temperate populations of calanoid copepods have multiple generations in a single year with variability in the timing of reproduction and development that causes significant heterogeneity in age structure. Thus, field sampling of these heterogeneous populations yields a mixture of copepods that are preparing for diapause, are preparing for the terminal molt, or are not yet preparing for either fate. Studies of diapause preparation in such populations are extremely difficult.

To enable direct investigation of the factors that influence diapause initiation, we require markers that reliably predict the fate of individual copepods (entry into diapause or continued maturation to adulthood) within heterogeneous populations. Fortunately, the fjord population of C. finmarchicus off the coast of Norway during the late spring is remarkably synchronous and is comprised of juvenile copepods that are all destined to undergo diapause. This project will use high-throughput 454 pyrosequencing to identify genes that change in expression as these copepods progress toward diapause. In collaboration with Norwegian researchers, the investigators will also rear copepods in a unique continuous culture and conduct additional transcriptional profiling to identify genes that change in expression as copepods prepare for the terminal molt. Comparison of gene expression patterns in the wild and cultured populations will enable the principal investigators to develop robust markers of diapauses preparation that can be used to study diapause initiation in more heterogeneous temperate populations.

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Funding SourceAward
NSF Division of Ocean Sciences (NSF OCE)

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