http://lod.bco-dmo.org/id/dataset/552923
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2015-03-04
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Chlorophyll-a concentrations from Barbados incubation experiment, February 2012 (ADIMA project)
2015-03-03
publication
2015-03-03
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2021-04-12
publication
https://doi.org/10.26008/1912/bco-dmo.552923.1
Adina Paytan
University of California-Santa Cruz
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Paytan, A. (2021) Chlorophyll-a concentrations from Barbados incubation experiment, February 2012 (ADIMA project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2015-03-03 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.552923.1 [access date]
Chlorophyll a Concentrations in Seawater Dataset Description: Methods and Sampling: <p><strong>Sampling and Analytical Methodology:</strong></p>
<p>Nutrient and aerosol addition bioassay experiments were carried out over 3 days in February 2012. Seawater was collected from offshore (water depth &gt;700 m) outside the Bellairs Research Institute at West Barbados (13<sup>o </sup>11.309’N, 59<sup>o </sup>38.267’W). Surface water was pumped into acid cleaned sample rinsed carboys using a peristaltic pump with acid washed Teflon tubing and pre filtered through a 50 um mesh acid washed Nitex<sup>©</sup> net to remove grazers. The seawater was stored in the dark until transport to the lab (within &lt;2 hours). Seawater was dispensed into acid washed and sample rinsed polycarbonate bottles (500 mL each), pre-labeled with treatment type (12-20 bottles per treatment). Treatments included single nutrient (N, P, Fe) additions as well as a combination of N and P and a combination of N and Fe at concentrations representative of deep water in this area. Three aerosol treatments were used in this study representing aerosols deposited in three seasons, winter, spring and summer. Aerosols representing each of the seasons were added at concentrations simulating high and low deposition rates. High deposition was calculated to represent the cumulative deposition flux over 10 days of a strong dust storm event over the North Atlantic (300 g m<sup>-2</sup> yr<sup>-1</sup>) to the upper 10 m mixed layer. Low deposition treatments were equivalent to the normal average deposition rate for Barbados (10 g m<sup>-2</sup> yr<sup>-1</sup>) during spring and summer. A control (no addition, blank filter) treatment and procedural blanks (Milli-Q water) were also included. All bottles were incubated in a pool filled with circulating seawater to maintain local surface ocean temperature. The pool was covered with a neutral density shading screen to reduce light intensity by 50%. Water samples used for the experiment (pre additions) was collected to characterize the baseline conditions (baseline, 5 replicates) and 3 replicate bottles for each treatment were also collected immediately after the additions were administered (time zero, t0). The experiment took place over 3 days, and each day 3 (for nutrients) or 5 (for aerosols) randomly selected bottles for each treatment were collected at 4pm in the afternoon (e.g. time points t1-t3). Immediately upon collection each bottle was sampled for chlorophyll <em>a</em>, flow cytometry, nutrients, and trace metal concentrations.</p>
<p>100 mL incubation seawater from each sample was filtered onto GFF filters and frozen in liquid nitrogen until analyzed. Upon return from the field samples were extracted for 24 hours in the dark at -20°C in 90% acetone. Fluorescence was measured on a TD-700 fluorometer (Turner Designs) calibrated with fresh chlorophyll standard from <em>Anacystis nidulans</em> (Sigma).</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-0850467 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=0850467
completed
Adina Paytan
University of California-Santa Cruz
831-459-1437
Earth & Marine Sciences C308 1156 High St
Santa Cruz
CA
95064
USA
apaytan@ucsc.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Experiment_Site
Lat
Lon
ID
Treatment
Time_Point
Chl_a
Average
Standard_Error
TD-700
theme
None, User defined
experiment id
latitude
longitude
sample identification
treatment
time_point
chlorophyll a
standard error
featureType
BCO-DMO Standard Parameters
Turner Designs 700 Laboratory Fluorometer
instrument
BCO-DMO Standard Instruments
ADIMA_Barbados
service
Deployment Activity
Bellairs Research Institute at West Barbados
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Atmospheric Deposition Impacts on Marine Ecosystems
http://pmc.ucsc.edu/~apaytan/page_projects.html
Atmospheric Deposition Impacts on Marine Ecosystems
<p>Chemical components delivered to the surface ocean through atmospheric deposition influence ocean productivity and ecosystem structure thus are tightly related to the global carbon cycle and climate. Accordingly, the major aim of this project is to quantitatively estimate the variable impact of aerosols on marine phytoplankton and to determine the specific effects on various taxa. Such data could in the future be used to better understand the global impact of aerosols on the oceanic ecosystem. To accomplish this goal the PI will monitor aerosol dry deposition fluxes, determine aerosol sources, obtain the chemical composition and solubility of aerosols, and evaluate the contribution of aerosols to nutrient and trace metal budgets of seawater at two oceanographically different sites (Bermuda and Monterey Bay) representing open ocean and coastal setting. The effects of the different aerosol "types" (defined by source and chemical characteristics) on specific phytoplankton taxa will also be evaluated using pure culture and natural samples bioassays. This project is particularly important in light of the role atmospheric deposition can resume in oligotrophic and coastal settings and the predicted future global conditions of increased aridity and urbanization and associated changes in dust fluxes and composition.</p>
ADIMA
largerWorkCitation
project
eng; USA
oceans
Bellairs Research Institute at West Barbados
-59.6406
-59.6406
13.1919
13.1919
2012-02-01
2012-02-29
Gulf of Aqaba, Atlantic Ocean (Bermuda Time Series Station), Monterey Bay
0
BCO-DMO catalogue of parameters from Chlorophyll-a concentrations from Barbados incubation experiment, February 2012 (ADIMA project)
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/552943.rdf
Name: Experiment_Site
Units: text
Description: <p>Identifier where experiments were conducted</p>
http://lod.bco-dmo.org/id/dataset-parameter/552944.rdf
Name: Lat
Units: decimal degrees
Description: <p>Approximate Latitude Position of Experiment Site; South is negative</p>
http://lod.bco-dmo.org/id/dataset-parameter/552945.rdf
Name: Lon
Units: decimal degrees
Description: <p>Approximate Longitude Position of Experiment Site; West is negative</p>
http://lod.bco-dmo.org/id/dataset-parameter/552946.rdf
Name: ID
Units: text
Description: <p>Sample Id</p>
http://lod.bco-dmo.org/id/dataset-parameter/552947.rdf
Name: Treatment
Units: text
Description: <p>Treatments</p>
http://lod.bco-dmo.org/id/dataset-parameter/552948.rdf
Name: Time_Point
Units: days
Description: <p>Experiment time point</p>
http://lod.bco-dmo.org/id/dataset-parameter/552949.rdf
Name: Chl_a
Units: ug/L
Description: <p>Chlorophyll a concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/552950.rdf
Name: Average
Units: ug/L
Description: <p>Average Chlorophyll a concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/552951.rdf
Name: Standard_Error
Units: (na)
Description: <p>Chlorophyll a concentration standard error</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
15724
https://darchive.mblwhoilibrary.org/bitstream/1912/26938/1/dataset-552923_chlorophyll-concentrations-seawater__v1.tsv
download
https://doi.org/10.26008/1912/bco-dmo.552923.1
download
onLine
dataset
<p><strong>Sampling and Analytical Methodology:</strong></p>
<p>Nutrient and aerosol addition bioassay experiments were carried out over 3 days in February 2012. Seawater was collected from offshore (water depth &gt;700 m) outside the Bellairs Research Institute at West Barbados (13<sup>o </sup>11.309’N, 59<sup>o </sup>38.267’W). Surface water was pumped into acid cleaned sample rinsed carboys using a peristaltic pump with acid washed Teflon tubing and pre filtered through a 50 um mesh acid washed Nitex<sup>©</sup> net to remove grazers. The seawater was stored in the dark until transport to the lab (within &lt;2 hours). Seawater was dispensed into acid washed and sample rinsed polycarbonate bottles (500 mL each), pre-labeled with treatment type (12-20 bottles per treatment). Treatments included single nutrient (N, P, Fe) additions as well as a combination of N and P and a combination of N and Fe at concentrations representative of deep water in this area. Three aerosol treatments were used in this study representing aerosols deposited in three seasons, winter, spring and summer. Aerosols representing each of the seasons were added at concentrations simulating high and low deposition rates. High deposition was calculated to represent the cumulative deposition flux over 10 days of a strong dust storm event over the North Atlantic (300 g m<sup>-2</sup> yr<sup>-1</sup>) to the upper 10 m mixed layer. Low deposition treatments were equivalent to the normal average deposition rate for Barbados (10 g m<sup>-2</sup> yr<sup>-1</sup>) during spring and summer. A control (no addition, blank filter) treatment and procedural blanks (Milli-Q water) were also included. All bottles were incubated in a pool filled with circulating seawater to maintain local surface ocean temperature. The pool was covered with a neutral density shading screen to reduce light intensity by 50%. Water samples used for the experiment (pre additions) was collected to characterize the baseline conditions (baseline, 5 replicates) and 3 replicate bottles for each treatment were also collected immediately after the additions were administered (time zero, t0). The experiment took place over 3 days, and each day 3 (for nutrients) or 5 (for aerosols) randomly selected bottles for each treatment were collected at 4pm in the afternoon (e.g. time points t1-t3). Immediately upon collection each bottle was sampled for chlorophyll <em>a</em>, flow cytometry, nutrients, and trace metal concentrations.</p>
<p>100 mL incubation seawater from each sample was filtered onto GFF filters and frozen in liquid nitrogen until analyzed. Upon return from the field samples were extracted for 24 hours in the dark at -20°C in 90% acetone. Fluorescence was measured on a TD-700 fluorometer (Turner Designs) calibrated with fresh chlorophyll standard from <em>Anacystis nidulans</em> (Sigma).</p>
Specified by the Principal Investigator(s)
<p><strong>Data Processing:</strong><br />
BD: Below detection limit</p>
<p><strong>BCO-DMO Processing Notes</strong><br />
&nbsp;- Generated from original file: "Data_Chlorophyll a concentrations from Barbados incubation experiment.xlsx" contributed by Chia-Te Chien<br />
&nbsp;- Parameter names edited to conform to BCO-DMO naming convention found at <a href="http://usjgofs.whoi.edu/naming-guidelines.html" target="_blank">Choosing Parameter Name</a><br />
&nbsp;- Common parameter names standardized between the four contributed Barbados datasets<br />
&nbsp;- Experiment Site Id and Lat/Lon appended to enable data discovery in MapServer<br />
&nbsp;- "nd" (no data) inserted into blank fields</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
TD-700
TD-700
PI Supplied Instrument Name: TD-700 PI Supplied Instrument Description:Fluorescence was measured on a TD-700 fluorometer (Turner Designs) calibrated with fresh chlorophyll standard from Anacystis nidulans (Sigma). Instrument Name: Turner Designs 700 Laboratory Fluorometer Instrument Short Name:TD-700 Instrument Description: The TD-700 Laboratory Fluorometer is a benchtop fluorometer designed to detect fluorescence over the UV to red range. The instrument can measure concentrations of a variety of compounds, including chlorophyll-a and fluorescent dyes, and is thus suitable for a range of applications, including chlorophyll, water quality monitoring and fluorescent tracer studies. Data can be output as concentrations or raw fluorescence measurements. Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0510/
Deployment: ADIMA_Barbados
ADIMA_Barbados
lab Bellairs Research Institute
lab Bellairs Research Institute
laboratory
ADIMA_Barbados
Adina Paytan
University of California-Santa Cruz
lab Bellairs Research Institute
lab Bellairs Research Institute
laboratory