http://lod.bco-dmo.org/id/dataset/555826
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2015-04-14
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Bacterial production data from R/V Knorr cruise KN207-01 along the southern tip of Nova Scotia to Bermuda in 2012 (SargassoSeaLipids project)
2015-04-14
publication
2015-04-14
revision
BCO-DMO Linked Data URI
2015-04-14
creation
http://lod.bco-dmo.org/id/dataset/555826
Benjamin A.S. Van Mooy
Woods Hole Oceanographic Institution
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Van Mooy, B. A. (2015) Bacterial production data from R/V Knorr cruise KN207-01 along the southern tip of Nova Scotia to Bermuda in 2012 (SargassoSeaLipids project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 14 April 2015) Version Date 2015-04-14 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/555826 [access date]
Bacterial production data from the KN207-01 cruise. Dataset Description: <p>Water column bacterial production (BP) rates measured using the 3H-leucine incorporation microcentrifuge method.</p> Methods and Sampling: <p>Refer to Collins et al., <em>Global Biogeochem. Cycles </em>(2015), in review. Excerpted from methods section:</p>
<p>Water column bacterial production (BP) rates were measured using the 3H-leucine incorporation microcentrifuge method of Simon and Azam (1989), as modified by Kirchman (2001). Incubations were conducted following each CTD cast using water samples from six depths; the first and sixth samples were always from the immediate surface layer (3-5 m) and 150 m. Triplicate 1 mL samples from each chosen depth or net trap were incubated with 3H-leucine (PerkinElmer, Inc., Waltham, MA; 146.5 Ci mmol-1, diluted to achieve 20 nM final concentration) for 4-12 hours at the temperature of the mixed layer. At the conclusion of the cruise, samples were processed and analyzed in a laboratory ashore according to Kirchman (2001) using Ultima Gold Low-Level Tritium cocktail (PerkinElmer, Inc.). Decay per minute counts in killed control samples were subtracted from the mean of each set of triplicates and divided by the incubation time to obtain a blank-corrected leucine incorporation rate in units of pmol leucine L-1 h-1.</p>
<p><strong>References:</strong><br />
Collins, J. R., B. R. Edwards, K. Thamatrakoln, J. E. Ossolinksi, G. R. DiTullio, K. D. Bidle, S. C. Doney, and B. A. S. Van Mooy (2015), The multiple fates of sinking particles in the North Atlantic Ocean, <em>Global Biogeochem. Cycles, </em>in review.</p>
<p>Simon, M., and F. Azam (1989), Protein-content and protein-synthesis rates of planktonic marine bacteria, <em>Mar. Ecol. Prog. Ser.</em>, <em>51</em>(3), 201-213, doi:<a href="http://dx.doi.org/10.3354/Meps051201" target="_blank">10.3354/Meps051201</a>.</p>
<p>Kirchman, D. (2001), Measuring bacterial biomass production and growth rates from leucine incorporation in natural aquatic environments, in <em>Methods in Microbiology</em>, edited by J. H. Paul, pp. 227-237, Academic Press, doi:<a href="http://dx.doi.org/10.1016/S0580-9517(01)30047-8" target="_blank">10.1016/S0580-9517(01)30047-8</a>.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1031143 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1031143
completed
Benjamin A.S. Van Mooy
Woods Hole Oceanographic Institution
(508) 289 2322
Department of Marine Chemistry and Geochemistry, MS #4, Fye 117 Woods Hole Oceanographic Institution
Woods Hole
MA
02543
USA
bvanmooy@whoi.edu
pointOfContact
asNeeded
Dataset Version: 14 April 2015
Unknown
station_ctd
incub_duration
ISO_DateTime_UTC
lat_CTD
lon_CTD
depth
DPM_live_samples_mean
DPM_live_samples_stdev
DPM_of_killed_control
trit_leu_uptake_live_mean_dpm
trit_leu_uptake_live_stdev_dpm
trit_leu_uptake_killed_dpm
trit_leu_uptake_dpm
trit_leu_uptake_stdev_dpm
trit_leu_uptake_live_mean_pmol
trit_leu_uptake_live_stdev_pmol
trit_leu_uptake_killed_pmol
trit_leu_uptake_pmol
trit_leu_uptake_stdev_pmol
signal_to_noise
theme
None, User defined
station number
incubation time or duration
ISO_DateTime_UTC
latitude
longitude
depth
No BCO-DMO term
featureType
BCO-DMO Standard Parameters
KN207-01
service
Deployment Activity
southern tip of Nova Scotia to Bermuda
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Ocean Carbon and Biogeochemistry
http://us-ocb.org/
Ocean Carbon and Biogeochemistry
The Ocean Carbon and Biogeochemistry (OCB) program focuses on the ocean's role as a component of the global Earth system, bringing together research in geochemistry, ocean physics, and ecology that inform on and advance our understanding of ocean biogeochemistry. The overall program goals are to promote, plan, and coordinate collaborative, multidisciplinary research opportunities within the U.S. research community and with international partners. Important OCB-related activities currently include: the Ocean Carbon and Climate Change (OCCC) and the North American Carbon Program (NACP); U.S. contributions to IMBER, SOLAS, CARBOOCEAN; and numerous U.S. single-investigator and medium-size research projects funded by U.S. federal agencies including NASA, NOAA, and NSF.
The scientific mission of OCB is to study the evolving role of the ocean in the global carbon cycle, in the face of environmental variability and change through studies of marine biogeochemical cycles and associated ecosystems.
The overarching OCB science themes include improved understanding and prediction of: 1) oceanic uptake and release of atmospheric CO2 and other greenhouse gases and 2) environmental sensitivities of biogeochemical cycles, marine ecosystems, and interactions between the two.
The OCB Research Priorities (updated January 2012) include: ocean acidification; terrestrial/coastal carbon fluxes and exchanges; climate sensitivities of and change in ecosystem structure and associated impacts on biogeochemical cycles; mesopelagic ecological and biogeochemical interactions; benthic-pelagic feedbacks on biogeochemical cycles; ocean carbon uptake and storage; and expanding low-oxygen conditions in the coastal and open oceans.
OCB
largerWorkCitation
program
Biogeochemical Impact and Fate of Non-phosphorus Membrane Lipids in the Sargasso Sea
https://osprey.bco-dmo.org/project/2189
Biogeochemical Impact and Fate of Non-phosphorus Membrane Lipids in the Sargasso Sea
<p>Intact polar diacyglycerols (IP-DAGs) are the fatty-acid bearing lipid molecules that compose bacterial and eukaryotic cell membranes. As such, they are one of the most abundant classes of lipid molecules in plankton, and play a major role in the marine carbon cycle. However, until very recently, the molecular diversity of IP-DAGs was poorly understood; the structural identity and characteristics of IP-DAGs were inferred almost exclusively from their constituent fatty acids. These non-phosphorus containing IP-DAGs were largely unknown to chemical oceanography. In contrast, phospholipids, which have been the focus of considerable research, compose a disproportionally small fraction of total IP-DAGs. But we still lack even a cursory understanding of biochemical functions and geochemical fates of non-phosphorus IP-DAGs. Given that these molecules are among the most abundant lipid molecules on the planet, this represents a profound and unexpected gap in our understanding the marine carbon and phosphorus cycles.</p>
<p>In this project, researchers at the Woods Hole Oceanographic Institution will launch a pioneering study of these poorly understood compounds. Their approach will be guided by four questions: (1) How do non-phosphorus lipids contribute to variations in the C:N:P of particulate organic matter in the Sargasso Sea? (2) What are the relative degradation rates of phospholipids and non-phosphorus lipids in surface waters? (3) Which groups of microbes utilize the carbon and phosphorus from different IP-DAGs? (4) What are the relative contributions of different IP-DAGs to particulate organic matter export to the deep-sea? </p>
<p>These questions will be answered by using sophisticated HPLC/MS analyses and novel isotope tracing approaches in conjunction with long-standing methods for measuring the C:N:P of plankton and determining the degradation rates of organic molecules. The research team will establish whether these newly-recognized sulfolipids and betaine lipids molecules are a quantitatively important biochemical option for phytoplankton to affect flexible C:N:P stoichiometry in the face of nutrient stress. They will also elucidate the degradation rate, microbial fate, and export potential of the carbon and phosphorus from IP-DAGs. This will shed new light on the broader roles of these molecules in the cycling of these elements by the planktonic community. </p>
<p>This project contains components that are specifically designed to meet the NSF criteria for "advancing discovery and understanding while promoting teaching, training and learning." The project will support the training of a graduate student and postdoctoral fellow. In addition, the research team will work with the non-profit Zephyr Foundation in Woods Hole to design educational 'units' based on the team's research that will be tailored to student in grades 6 - 12. The Foundation will present these units as part of their hands-on marine science field trip series that is delivered to over 200 students and their teachers per year.</p>
SargassoSeaLipids
largerWorkCitation
project
eng; USA
oceans
southern tip of Nova Scotia to Bermuda
2015-04-14
Sargasso Sea
0
BCO-DMO catalogue of parameters from Bacterial production data from R/V Knorr cruise KN207-01 along the southern tip of Nova Scotia to Bermuda in 2012 (SargassoSeaLipids project)
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/555836.rdf
Name: station_ctd
Units: dimensionless
Description: <p>CTD station number.</p>
http://lod.bco-dmo.org/id/dataset-parameter/555837.rdf
Name: incub_duration
Units: minutes
Description: <p>Incubation duration.</p>
http://lod.bco-dmo.org/id/dataset-parameter/555838.rdf
Name: ISO_DateTime_UTC
Units: YYYY-mm-ddTHH:MM:SS.xxZ
Description: <p>Date and time (from CTD timestamp) formatted to ISO 8601 standard. T represents the start of the time string and Z indicates UTC.</p>
http://lod.bco-dmo.org/id/dataset-parameter/555839.rdf
Name: lat_CTD
Units: decimal degrees
Description: <p>Latitude of CTD station.</p>
http://lod.bco-dmo.org/id/dataset-parameter/555840.rdf
Name: lon_CTD
Units: decimal degrees
Description: <p>Longitude of CTD station.</p>
http://lod.bco-dmo.org/id/dataset-parameter/555841.rdf
Name: depth
Units: meters (m)
Description: <p>Sample depth.</p>
http://lod.bco-dmo.org/id/dataset-parameter/555842.rdf
Name: DPM_live_samples_mean
Units: dpm (disintegrations per minute)
Description: <p>Mean DPM (disintegrations per minute), live samples.</p>
http://lod.bco-dmo.org/id/dataset-parameter/555843.rdf
Name: DPM_live_samples_stdev
Units: dpm (disintegrations per minute)
Description: <p>Standard deviation DPM (disintegrations per minute), live samples.</p>
http://lod.bco-dmo.org/id/dataset-parameter/555844.rdf
Name: DPM_of_killed_control
Units: dpm (disintegrations per minute)
Description: <p>DPM (disintegrations per minute) of killed control.</p>
http://lod.bco-dmo.org/id/dataset-parameter/555845.rdf
Name: trit_leu_uptake_live_mean_dpm
Units: dpm per liter per hour (dpm/L/hr)
Description: <p>Mean tritium-labeled leucine (3H-Leu) uptake, live samples.</p>
http://lod.bco-dmo.org/id/dataset-parameter/555846.rdf
Name: trit_leu_uptake_live_stdev_dpm
Units: dpm per liter per hour (dpm/L/hr)
Description: <p>Standard deviation of tritium-labeled leucine (3H-Leu) uptake, live samples.</p>
http://lod.bco-dmo.org/id/dataset-parameter/555847.rdf
Name: trit_leu_uptake_killed_dpm
Units: dpm per liter per hour (dpm/L/hr)
Description: <p>Tritium-labeled leucine (3H-Leu) uptake, killed control.</p>
http://lod.bco-dmo.org/id/dataset-parameter/555848.rdf
Name: trit_leu_uptake_dpm
Units: dpm per liter per hour (dpm/L/hr)
Description: <p>Tritium-labeled leucine (3H-Leu) uptake.</p>
http://lod.bco-dmo.org/id/dataset-parameter/555849.rdf
Name: trit_leu_uptake_stdev_dpm
Units: dpm per liter per hour (dpm/L/hr)
Description: <p>Standard deviation of tritium-labeled leucine (3H-Leu) uptake.</p>
http://lod.bco-dmo.org/id/dataset-parameter/555850.rdf
Name: trit_leu_uptake_live_mean_pmol
Units: picomoles per liter per hour (pmol/L/hr)
Description: <p>Mean tritium-labeled leucine (3H-Leu) uptake, live samples.</p>
http://lod.bco-dmo.org/id/dataset-parameter/555851.rdf
Name: trit_leu_uptake_live_stdev_pmol
Units: picomoles per liter per hour (pmol/L/hr)
Description: <p>Standard deviation tritium-labeled leucine (3H-Leu) uptake, live samples.</p>
http://lod.bco-dmo.org/id/dataset-parameter/555852.rdf
Name: trit_leu_uptake_killed_pmol
Units: picomoles per liter per hour (pmol/L/hr)
Description: <p>Tritium-labeled leucine (3H-Leu) uptake, killed control.</p>
http://lod.bco-dmo.org/id/dataset-parameter/555853.rdf
Name: trit_leu_uptake_pmol
Units: picomoles 3H-leucine per liter per hour (pmol 3H-leu/L/hr)
Description: <p>The final, blank-corrected, replicate-averaged rates of leucine incorporation for each CTD station and depth.</p>
http://lod.bco-dmo.org/id/dataset-parameter/555854.rdf
Name: trit_leu_uptake_stdev_pmol
Units: picomoles 3H-leucine per liter per hour (pmol 3H-leu/L/hr)
Description: <p>Standard deviation of the final, blank-corrected, replicate-averaged rates of leucine incorporation for each CTD station and depth.</p>
http://lod.bco-dmo.org/id/dataset-parameter/555855.rdf
Name: signal_to_noise
Units: dimensionless (ratio)
Description: <p>The "signal to noise" ratio for each measurement, i.e., the ratio of the incorporation rate in the live samples to the killed control at that depth.</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
29897
https://datadocs.bco-dmo.org/file/yppz40wC2LZMkL/KN207-01_bac_prod.csv
KN207-01_bac_prod.csv
Primary data file for dataset ID 555826
download
https://osprey.bco-dmo.org/dataset/555826/data/download
download
onLine
dataset
<p>Refer to Collins et al., <em>Global Biogeochem. Cycles </em>(2015), in review. Excerpted from methods section:</p>
<p>Water column bacterial production (BP) rates were measured using the 3H-leucine incorporation microcentrifuge method of Simon and Azam (1989), as modified by Kirchman (2001). Incubations were conducted following each CTD cast using water samples from six depths; the first and sixth samples were always from the immediate surface layer (3-5 m) and 150 m. Triplicate 1 mL samples from each chosen depth or net trap were incubated with 3H-leucine (PerkinElmer, Inc., Waltham, MA; 146.5 Ci mmol-1, diluted to achieve 20 nM final concentration) for 4-12 hours at the temperature of the mixed layer. At the conclusion of the cruise, samples were processed and analyzed in a laboratory ashore according to Kirchman (2001) using Ultima Gold Low-Level Tritium cocktail (PerkinElmer, Inc.). Decay per minute counts in killed control samples were subtracted from the mean of each set of triplicates and divided by the incubation time to obtain a blank-corrected leucine incorporation rate in units of pmol leucine L-1 h-1.</p>
<p><strong>References:</strong><br />
Collins, J. R., B. R. Edwards, K. Thamatrakoln, J. E. Ossolinksi, G. R. DiTullio, K. D. Bidle, S. C. Doney, and B. A. S. Van Mooy (2015), The multiple fates of sinking particles in the North Atlantic Ocean, <em>Global Biogeochem. Cycles, </em>in review.</p>
<p>Simon, M., and F. Azam (1989), Protein-content and protein-synthesis rates of planktonic marine bacteria, <em>Mar. Ecol. Prog. Ser.</em>, <em>51</em>(3), 201-213, doi:<a href="http://dx.doi.org/10.3354/Meps051201" target="_blank">10.3354/Meps051201</a>.</p>
<p>Kirchman, D. (2001), Measuring bacterial biomass production and growth rates from leucine incorporation in natural aquatic environments, in <em>Methods in Microbiology</em>, edited by J. H. Paul, pp. 227-237, Academic Press, doi:<a href="http://dx.doi.org/10.1016/S0580-9517(01)30047-8" target="_blank">10.1016/S0580-9517(01)30047-8</a>.</p>
Specified by the Principal Investigator(s)
<p>The "signal_to_noise" column gives the ratio for each measurement, i.e., the ratio of the incorporation rate in the live samples to the killed control at that depth.</p>
<p>BCO-DMO processing notes:<br />
- Replaced 'NaN' with 'nd' to indicate 'no data'.<br />
- Modified parameter names to conform with BCO-DMO naming conventions.<br />
- Modified format of date/time to fit ISO8601 format.</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Cruise: KN207-01
KN207-01
R/V Knorr
Community Standard Description
International Council for the Exploration of the Sea
R/V Knorr
vessel
KN207-01
Benjamin A.S. Van Mooy
Woods Hole Oceanographic Institution
R/V Knorr
Community Standard Description
International Council for the Exploration of the Sea
R/V Knorr
vessel