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            <gco:CharacterString>Cite this dataset as: Van Mooy, B. A. (2015) Sinking POC and PIC fluxes measured with PIT sediment traps R/V Knorr cruise KN207-01 along the southern tip of Nova Scotia to Bermuda in 2012 (SargassoSeaLipids project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 15 April 2015) Version Date 2015-04-15 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/555879 [access date]</gco:CharacterString>
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        <gco:CharacterString>Sinking POC and PIC fluxes measured with PIT sediment traps on KN207-01 cruise. Dataset Description: &amp;lt;p&amp;gt;Sinking POC and PIC fluxes measured with PIT sediment traps on the KN207-01 cruise.&amp;lt;/p&amp;gt; Methods and Sampling: &amp;lt;p&amp;gt;Refer to Collins et al., &amp;lt;em&amp;gt;Global Biogeochem. Cycles &amp;lt;/em&amp;gt;(2015), in review. Excerpted from methods section:&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Vertically sinking particulate carbon fluxes were measured at 50, 150, and 300 meters using surface-tethered cylindrical sediment traps (0.0125 m2 cross-sectional area; materials and construction as described in McDonnell and Buesseler, 2012). A mooring consisting of four traps at each depth, a surface buoy, wave-action mitigation bungee cord, and several floats, was deployed at each process station and allowed to drift for 3-5 days. The quasi-Lagrangian behavior of the mooring during each deployment was confirmed by comparison of positional data obtained from an Argos satellite beacon mounted on the surface buoy with shipboard acoustic Doppler current profiler (ADCP) data from the R/V Knorr, which trailed the mooring at a range of 1-2 miles.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Traps were prepared, deployed, and recovered as described in McDonnell and Buesseler (2012). Traps were then sampled for particulate carbon in accordance with McDonnell and Buesseler (2012), except that the screened brine suspension (350 um pore size, to exclude macrozooplankton) was filtered onto a series of precombusted, 47 mm GF/F filters (0.7 um nominal pore size). Field and analytical blanks were collected at each station. Filters were immediately frozen in liquid nitrogen and then stored at -80 degrees C.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Filters from three of the four traps at each depth were used for determination of total particulate and particulate inorganic carbon (TPC and PIC, respectively). After thawing, the filters (including blanks) were first dried at 70 degrees C in a drying oven; each filter was then weighed and cut in half with precombusted stainless steel scissors. Each half was then weighed separately. One half was reserved for PIC analysis and the other reserved for determination of TPC.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;For TPC, sets of filter halves were transferred to 12 mm by 20 cm precombusted quartz tubes containing copper oxide (100 mg) and elemental silver wires. The tubes were then attached to a vacuum line, evacuated, flame-sealed, and combusted at 850 degrees C for 10 h. The evolved carbon dioxide was then isolated through a series of cold traps and quantified manometrically. PIC was determined from the other set of filter halves by coulometric analysis of acidified samples using a Model CM5014 UIC Coulometric Analyzer with Carbonate Acidification Module, as described in Honjo et al. (1995). Particulate organic carbon (POC) was determined for each trap by difference of the blank-corrected TPC and PIC measurements.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;References:&amp;lt;/strong&amp;gt;&amp;lt;br /&amp;gt;
Collins, J. R., B. R. Edwards, K. Thamatrakoln, J. E. Ossolinksi, G. R. DiTullio, K. D. Bidle, S. C. Doney, and B. A. S. Van Mooy (2015), The multiple fates of sinking particles in the North Atlantic Ocean, &amp;lt;em&amp;gt;Global Biogeochem. Cycles, &amp;lt;/em&amp;gt;in review.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;McDonnell, A. M. P., and K. O. Buesseler (2012), A new method for the estimation of sinking particle fluxes from measurements of the particle size distribution, average sinking velocity, and carbon content, &amp;lt;em&amp;gt;Limnol Oceanogr-Meth&amp;lt;/em&amp;gt;, &amp;lt;em&amp;gt;10&amp;lt;/em&amp;gt;, 329-346, doi:&amp;lt;a href=&amp;quot;http://dx.doi.org/10.4319/Lom.2012.10.329&amp;quot; target=&amp;quot;_blank&amp;quot;&amp;gt;10.4319/Lom.2012.10.329&amp;lt;/a&amp;gt;.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Honjo, S., J. Dymond, R. Collier, and S. J. Manganini (1995), Export production of particles to the interior of the equatorial Pacific Ocean during the 1992 EqPac experiment, &amp;lt;em&amp;gt;DSR&amp;lt;/em&amp;gt;, &amp;lt;em&amp;gt;42&amp;lt;/em&amp;gt;(2–3), 831-870, doi:&amp;lt;a href=&amp;quot;http://dx.doi.org/10.1016/0967-0645(95)00034-N&amp;quot; target=&amp;quot;_blank&amp;quot;&amp;gt;10.1016/0967-0645(95)00034-N&amp;lt;/a&amp;gt;.&amp;lt;/p&amp;gt;</gco:CharacterString>
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                            <gco:CharacterString>&lt;p&gt;Intact polar diacyglycerols (IP-DAGs) are the fatty-acid bearing lipid  molecules that compose bacterial and eukaryotic cell membranes. As such,  they are one of the most abundant classes of lipid molecules in  plankton, and play a major role in the marine carbon cycle. However,  until very recently, the molecular diversity of IP-DAGs was poorly  understood; the structural identity and characteristics of IP-DAGs were  inferred almost exclusively from their constituent fatty acids.  These  non-phosphorus containing IP-DAGs were largely unknown to chemical  oceanography. In contrast, phospholipids, which have been the focus of  considerable research, compose a disproportionally small fraction of  total IP-DAGs.  But we still lack even a cursory understanding of  biochemical functions and geochemical fates of non-phosphorus IP-DAGs.  Given that these molecules are among the most abundant lipid molecules  on the planet, this represents a profound and unexpected gap in our  understanding the marine carbon and phosphorus cycles.&lt;/p&gt;
&lt;p&gt;In this  project, researchers at the Woods Hole Oceanographic Institution will  launch a pioneering study of these poorly understood compounds.  Their  approach will be guided by four questions:  (1) How do non-phosphorus  lipids contribute to variations in the C:N:P of particulate organic  matter in the Sargasso Sea?  (2) What are the relative degradation rates  of phospholipids and non-phosphorus lipids in surface waters?  (3)  Which groups of microbes utilize the carbon and phosphorus from  different IP-DAGs?  (4) What are the relative contributions of different  IP-DAGs to particulate organic matter export to the deep-sea? &lt;/p&gt;
&lt;p&gt;These  questions will be answered by using sophisticated HPLC/MS analyses and  novel isotope tracing approaches in conjunction with long-standing  methods for measuring the C:N:P of plankton and determining the  degradation rates of organic molecules.  The research team will  establish whether these newly-recognized sulfolipids and betaine lipids  molecules are a quantitatively important biochemical option for  phytoplankton to affect flexible C:N:P stoichiometry in the face of  nutrient stress. They will also elucidate the degradation rate,  microbial fate, and export potential of the carbon and phosphorus from  IP-DAGs. This will shed new light on the broader roles of these  molecules in the cycling of these elements by the planktonic community. &lt;/p&gt;
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&amp;lt;p&amp;gt;Vertically sinking particulate carbon fluxes were measured at 50, 150, and 300 meters using surface-tethered cylindrical sediment traps (0.0125 m2 cross-sectional area; materials and construction as described in McDonnell and Buesseler, 2012). A mooring consisting of four traps at each depth, a surface buoy, wave-action mitigation bungee cord, and several floats, was deployed at each process station and allowed to drift for 3-5 days. The quasi-Lagrangian behavior of the mooring during each deployment was confirmed by comparison of positional data obtained from an Argos satellite beacon mounted on the surface buoy with shipboard acoustic Doppler current profiler (ADCP) data from the R/V Knorr, which trailed the mooring at a range of 1-2 miles.&amp;lt;/p&amp;gt;

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&amp;lt;p&amp;gt;For TPC, sets of filter halves were transferred to 12 mm by 20 cm precombusted quartz tubes containing copper oxide (100 mg) and elemental silver wires. The tubes were then attached to a vacuum line, evacuated, flame-sealed, and combusted at 850 degrees C for 10 h. The evolved carbon dioxide was then isolated through a series of cold traps and quantified manometrically. PIC was determined from the other set of filter halves by coulometric analysis of acidified samples using a Model CM5014 UIC Coulometric Analyzer with Carbonate Acidification Module, as described in Honjo et al. (1995). Particulate organic carbon (POC) was determined for each trap by difference of the blank-corrected TPC and PIC measurements.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;References:&amp;lt;/strong&amp;gt;&amp;lt;br /&amp;gt;
Collins, J. R., B. R. Edwards, K. Thamatrakoln, J. E. Ossolinksi, G. R. DiTullio, K. D. Bidle, S. C. Doney, and B. A. S. Van Mooy (2015), The multiple fates of sinking particles in the North Atlantic Ocean, &amp;lt;em&amp;gt;Global Biogeochem. Cycles, &amp;lt;/em&amp;gt;in review.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;McDonnell, A. M. P., and K. O. Buesseler (2012), A new method for the estimation of sinking particle fluxes from measurements of the particle size distribution, average sinking velocity, and carbon content, &amp;lt;em&amp;gt;Limnol Oceanogr-Meth&amp;lt;/em&amp;gt;, &amp;lt;em&amp;gt;10&amp;lt;/em&amp;gt;, 329-346, doi:&amp;lt;a href=&amp;quot;http://dx.doi.org/10.4319/Lom.2012.10.329&amp;quot; target=&amp;quot;_blank&amp;quot;&amp;gt;10.4319/Lom.2012.10.329&amp;lt;/a&amp;gt;.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Honjo, S., J. Dymond, R. Collier, and S. J. Manganini (1995), Export production of particles to the interior of the equatorial Pacific Ocean during the 1992 EqPac experiment, &amp;lt;em&amp;gt;DSR&amp;lt;/em&amp;gt;, &amp;lt;em&amp;gt;42&amp;lt;/em&amp;gt;(2–3), 831-870, doi:&amp;lt;a href=&amp;quot;http://dx.doi.org/10.1016/0967-0645(95)00034-N&amp;quot; target=&amp;quot;_blank&amp;quot;&amp;gt;10.1016/0967-0645(95)00034-N&amp;lt;/a&amp;gt;.&amp;lt;/p&amp;gt;</gco:CharacterString>
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          <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/platform/53994.rdf"
           xlink:title="316N" xlink:actuate="onRequest">R/V Knorr</gmx:Anchor>
        </gmd:code>
      </gmd:MD_Identifier>
    </gmi:identifier>
    <gmi:description>
      <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/platform/53994.rdf" xlink:title="R/V Knorr" xlink:actuate="onRequest">vessel</gmx:Anchor>
    </gmi:description>
    <gmi:instrument gco:nilReason="unknown"/>
  </gmi:MI_Platform>
</gmi:platform>
          </gmi:MI_AcquisitionInformation>
  </gmi:acquisitionInformation>
</gmi:MI_Metadata>
