http://lod.bco-dmo.org/id/dataset/556033
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2015-04-17
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Data describing Tethered PaV1 infected juvenile lobsters introduced to occupied wild dens in the south-central Florida Bay (Lobster disease connectivity project)
2015-04-13
publication
2015-04-13
revision
BCO-DMO Linked Data URI
2015-04-13
creation
http://lod.bco-dmo.org/id/dataset/556033
Mark Butler
Old Dominion University
principalInvestigator
Donald Behringer
University of Florida
principalInvestigator
Claire B. Paris-Limouzy
University of Miami Rosenstiel School of Marine and Atmospheric Science
principalInvestigator
Jeffery Shields
Virginia Institute of Marine Science
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Butler, M., Behringer, D., Shields, J., Paris-Limouzy, C. B. (2015) Data describing Tethered PaV1 infected juvenile lobsters introduced to occupied wild dens in the south-central Florida Bay (Lobster disease connectivity project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 2015-04-13) Version Date 2015-04-13 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/556033 [access date]
Tethered PaV1 infected juvenile lobsters introduced to occupied wild dens Dataset Description: <p>Field experiment in which uninfected or PaV1-infected tethered juvenile lobsters were introduced into dens with natural aggregations of lobsters whose abundance was again observed 24 hrs later.</p>
<p><strong>Related References:</strong></p>
<p>Methods and results appear in the following publication: Butler, M.J. IV, D.C. Behringer Jr., T.W. Dolan III, J. Moss, and J.D. Shields. (in press, 04/2015) Behavioral immunity suppresses an epizootic in Caribbean lobsters. PLOS ONE.</p> Methods and Sampling: <p>We tested whether aggregations of healthy lobsters in natural dens differed in their response to the introduction into their den of an additional healthy lobster or a PaV1-infected lobster in a field experiment at three general locations in Florida Bay, Florida (USA)(approximate location coordinates: 24 56 29.72oN, 80 49 24.58oW; 24 50 30.55oN, 80 47 44.86oW; 24 50 17.04oN, 80 48 05.18oW) conducted on sites that had experienced a sponge die-off and thus where lobsters were highly aggregated.&nbsp; Lobsters (16 - 56 mm CL) were first collected by divers from natural habitats in the middle Florida Keys region, but not at the study site.&nbsp; The presence or absence of PaV1 in collected lobsters was assayed using PCR and all diseased lobsters used in the experiment were visibly diseased (i.e., showed clinical signs of infection). Lobsters were tethered by tying a loop of monofilament (8 kg test) around their carapace (between the second and third periopods) with a snap swivel tied on the dorsal side of the carapace and the knot sealed with cyanoacrylate superglue.&nbsp; The snap swivel was clipped to a brick by a 50 cm long monofilament line.&nbsp; This arrangement permitted tethered lobsters to move freely and naturally within the limits of the tether, but they could not escape.&nbsp; Divers introduced either a single healthy or single visibly diseased juvenile lobster into separate, haphazardly selected natural aggregations of lobsters found under coral heads or within rocky holes. Dens were individually marked with numbered surface buoys so they could be relocated.&nbsp; We counted and estimated the sizes of all lobsters within each den prior to the introduction of the tethered lobster and then again 24 hrs later. Healthy tethered lobsters were released after the study, whereas diseased lobsters were returned to the laboratory and euthanized to prevent further spread of the PaV1 virus.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-0928930 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=0928930
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-0929086 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=0929086
completed
Mark Butler
Old Dominion University
USA
mjbutleriv@gmail.com
pointOfContact
Donald Behringer
University of Florida
352-273-3634
UF School of Forest Resources and Conservation, Fisheries and Aquatic Sciences 7922 NW 71st Street
Gainesville
FL
32653
USA
behringer@ufl.edu
pointOfContact
Claire B. Paris-Limouzy
University of Miami Rosenstiel School of Marine and Atmospheric Science
305-421-4219
University of Miami Rosenstiel School of Marine and Atmospheric Science, Division of Marine Physics 4600 Rickenbacker Causeway
Miami
FL
33149-1098
USA
cparis@rsmas.miami.edu
pointOfContact
Jeffery Shields
Virginia Institute of Marine Science
(804) 684-7128
Virginia Institute of Marine Science
Gloucester Point
VA
23062
jeff@vims.edu
pointOfContact
asNeeded
Dataset Version: 2015-04-13
Unknown
site
date_start
lat
lon
den_id
den_type
treatment
length_carap_teth
sex_teth
num_lobsters_start
length_carap_natl
num_lobsters_24hrs
length_carap_natl_24hrs
live_dead_teth
theme
None, User defined
site
date_start
latitude
longitude
sample identification
sample description
treatment
length
sex
count
No BCO-DMO term
featureType
BCO-DMO Standard Parameters
Butler_FloridaBay
service
Deployment Activity
south-central Florida Bay
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Connectivity of disease in marine Ecosystems: multi-scale dynamics of a viral disease infecting caribbean spiny lobster
https://osprey.bco-dmo.org/project/472649
Connectivity of disease in marine Ecosystems: multi-scale dynamics of a viral disease infecting caribbean spiny lobster
<p>Scientists are struck by how different terrestrial epidemiology is from that in marine ecosystems, a crucial difference being the more rapid spread of diseases in the ocean due to the presumed absence of barriers to waterborne dispersal. Yet, the movement of pathogens in the sea and its importance to disease dynamics in marine metapopulations is virtually unstudied. Marine pathogens do spread among distant host populations, as demonstrated by dramatic epizootics, but is this common or demographically relevant? Nearly all studies of marine diseases treat such events as transitory, focusing instead on local disease dynamics. This approach suggests either that small-scale phenomena normally trump the influence of large-scale pathogen connectivity or, alternatively, that the dispersal of marine pathogens by highly motile adults or free-living waterborne pathogens is simply too intractable for empirical investigation. Yet, there is another perhaps unappreciated mechanism – dispersal by infected larvae. Most marine animals have life histories that include planktonic larvae, many of which are highly dispersive. If infected by pathogens, these “larval vectors” would provide an efficient mechanism for distributing pathogens at high concentrations directly into habitats where hosts dwell. More so than passive, waterborne pathogens that are subject to rapid dilution and have no means of targeting distant hosts.</p>
<p>We have evidence that long-distance pathogen dispersal in the sea via infected meroplanktonic larvae may be possible. The pathogen in question is an often lethal, pathogenic virus (PaV1; Panulirus argus virus 1) that infects the Caribbean spiny lobster, Panulirus argus – a species broadly distributed throughout the Caribbean where it supports the most valuable fishery in the region. We described the PaV1 virus in 1999 and since then have studied its pathology, epidemiology, transmission, and effects on juvenile lobster populations in the Florida Keys. Like others, our focus has been on local pathogen-host dynamics, but PaV1 infections in lobsters are now confirmed in distant areas of the Caribbean (Belize, Mexico, St. Croix) in regions that are demographically linked only by dispersing larvae that spend >6 mos. in the open ocean. We recently discovered that many lobster postlarvae recruiting to coastal nurseries in Florida are infected with PaV1, providing novel evidence for pathogen connectivity among distant host populations.</p>
<p>Focusing on the spiny lobster-PaV1 virus association as a case study, we propose an ambitious program of laboratory, field, and modeling research whose broader implications will better our understanding of the importance of dispersal by infectious agents on the spread and maintenance of disease in marine populations. The project builds upon data and techniques developed with prior NSF sponsorship, and brings together partners in developing Caribbean nations with a multidisciplinary group of scientists with long-standing research programs in larval biology, biophysical and ecological modeling, crustacean biology, molecular biology, and the study of marine diseases.</p>
Lobster disease connectivity
largerWorkCitation
project
eng; USA
biota
oceans
south-central Florida Bay
2015-04-13
Florida Keys, USA and Carrbbean
0
BCO-DMO catalogue of parameters from Data describing Tethered PaV1 infected juvenile lobsters introduced to occupied wild dens in the south-central Florida Bay (Lobster disease connectivity project)
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/556116.rdf
Name: site
Units: unitless
Description: <p>site of tethering</p>
http://lod.bco-dmo.org/id/dataset-parameter/556117.rdf
Name: date_start
Units: yyyy-mm-dd
Description: <p>date tethering began</p>
http://lod.bco-dmo.org/id/dataset-parameter/556118.rdf
Name: lat
Units: decimal degrees
Description: <p>latitude; north is positive</p>
http://lod.bco-dmo.org/id/dataset-parameter/556119.rdf
Name: lon
Units: decimal degrees
Description: <p>longitude; east is positive</p>
http://lod.bco-dmo.org/id/dataset-parameter/556120.rdf
Name: den_id
Units: unitless
Description: <p>den identification number</p>
http://lod.bco-dmo.org/id/dataset-parameter/556121.rdf
Name: den_type
Units: unitless
Description: <p>type of den structure: spl = loggerhead sponge; sol = solution hole; c = coral head; spf = finger sponge; rock = rock</p>
http://lod.bco-dmo.org/id/dataset-parameter/556122.rdf
Name: treatment
Units: unitless
Description: <p>tethered lobster treatment: either PaV1 diseased or healthy lobster</p>
http://lod.bco-dmo.org/id/dataset-parameter/556123.rdf
Name: length_carap_teth
Units: millimeters
Description: <p>carapace length of tethered lobster</p>
http://lod.bco-dmo.org/id/dataset-parameter/556124.rdf
Name: sex_teth
Units: unitless
Description: <p>sex of tethered lobster</p>
http://lod.bco-dmo.org/id/dataset-parameter/556125.rdf
Name: num_lobsters_start
Units: unit
Description: <p>number of naturally occurring lobsters observed in den at start of study</p>
http://lod.bco-dmo.org/id/dataset-parameter/556126.rdf
Name: length_carap_natl
Units: millimeters
Description: <p>estimated carapace length of lobsters observed in den at start</p>
http://lod.bco-dmo.org/id/dataset-parameter/556127.rdf
Name: num_lobsters_24hrs
Units: unit
Description: <p>number of naturally occurring lobsters observed in den after 24 hrs</p>
http://lod.bco-dmo.org/id/dataset-parameter/556128.rdf
Name: length_carap_natl_24hrs
Units: millimeters
Description: <p>estimated carapace length of lobsters observed in den after 24 hrs</p>
http://lod.bco-dmo.org/id/dataset-parameter/556129.rdf
Name: live_dead_teth
Units: unitless
Description: <p>whether tethered lobster alive or dead after 24 hrs</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
4696
https://datadocs.bco-dmo.org/file/5AAMv6RSg25qEy/tether.csv
tether.csv
Primary data file for dataset ID 556033
download
https://osprey.bco-dmo.org/dataset/556033/data/download
download
onLine
dataset
<p>We tested whether aggregations of healthy lobsters in natural dens differed in their response to the introduction into their den of an additional healthy lobster or a PaV1-infected lobster in a field experiment at three general locations in Florida Bay, Florida (USA)(approximate location coordinates: 24 56 29.72oN, 80 49 24.58oW; 24 50 30.55oN, 80 47 44.86oW; 24 50 17.04oN, 80 48 05.18oW) conducted on sites that had experienced a sponge die-off and thus where lobsters were highly aggregated.&nbsp; Lobsters (16 - 56 mm CL) were first collected by divers from natural habitats in the middle Florida Keys region, but not at the study site.&nbsp; The presence or absence of PaV1 in collected lobsters was assayed using PCR and all diseased lobsters used in the experiment were visibly diseased (i.e., showed clinical signs of infection). Lobsters were tethered by tying a loop of monofilament (8 kg test) around their carapace (between the second and third periopods) with a snap swivel tied on the dorsal side of the carapace and the knot sealed with cyanoacrylate superglue.&nbsp; The snap swivel was clipped to a brick by a 50 cm long monofilament line.&nbsp; This arrangement permitted tethered lobsters to move freely and naturally within the limits of the tether, but they could not escape.&nbsp; Divers introduced either a single healthy or single visibly diseased juvenile lobster into separate, haphazardly selected natural aggregations of lobsters found under coral heads or within rocky holes. Dens were individually marked with numbered surface buoys so they could be relocated.&nbsp; We counted and estimated the sizes of all lobsters within each den prior to the introduction of the tethered lobster and then again 24 hrs later. Healthy tethered lobsters were released after the study, whereas diseased lobsters were returned to the laboratory and euthanized to prevent further spread of the PaV1 virus.</p>
Specified by the Principal Investigator(s)
<p>Data analyzed using a 2-way log-linear contingency table analysis to test for independence of the effect of PaV1 infection and tendancy for den aggregation to decrease or to increase/remain the same.&nbsp;&nbsp;</p>
<p><strong>BCO-DMO Processing:</strong></p>
<p>- added conventional header with dataset name, PI name, version date, reference information<br />
- renamed parameters to BCO-DMO standard<br />
- added lat, lon columns<br />
- reformatted date from m/d/yyyy to yyyy-mm-dd<br />
- replaced blank cells with nd</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Deployment: Butler_FloridaBay
Butler_FloridaBay
Lobster habitat
shoreside
Butler_FloridaBay
Mark Butler
Old Dominion University
Lobster habitat
shoreside