http://lod.bco-dmo.org/id/dataset/556162
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2015-04-20
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Data describing Long-term PaV1 lobster surveys in the south-central Florida Bay from 1999-2014 (Lobster disease connectivity project)
2015-04-13
publication
2015-04-13
revision
BCO-DMO Linked Data URI
2015-04-13
creation
http://lod.bco-dmo.org/id/dataset/556162
Mark Butler
Old Dominion University
principalInvestigator
Donald Behringer
University of Florida
principalInvestigator
Claire B. Paris-Limouzy
University of Miami Rosenstiel School of Marine and Atmospheric Science
principalInvestigator
Jeffery Shields
Virginia Institute of Marine Science
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Butler, M., Behringer, D., Shields, J., Paris-Limouzy, C. B. (2015) Data describing Long-term PaV1 lobster surveys in the south-central Florida Bay from 1999-2014 (Lobster disease connectivity project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 2015-04-13) Version Date 2015-04-13 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/556162 [access date]
Long-term PaV1 lobster surveys Florida bayside, 1999-2014 Dataset Description: <p>From 1999-2014, lobsters were briefly captured in Florida Bay. Measurements taken included sex, length, molt condition, evidence of injury and disease. &nbsp;PaV1 virus&nbsp;PCR results&nbsp;were logged.</p>
<p><strong>Related References:</strong></p>
<p>Portions of these data have been reported in the following publication:</p>
<p>Behringer, D.C., M.J. Butler, J. Shields, and J. Moss. 2011. A review of Panulirus argus Virus 1 – a Decade after its Discovery. Diseases of Aquatic Organisms 94: 153–160.</p> Methods and Sampling: <p>At each permanent 25m x 25m sample location (hard-bottom habitat 1.5 – 3.0 m depth), delineated by rope border affixed to seafloor, two divers search the entire site for spiny lobsters (Panulirus argus).&nbsp; All lobsters sighted are collected in hand nets by divers and brought aboard the topside vessel where the data are collected &amp; recorded.&nbsp; Lobsters are then returned live to the original site.&nbsp; Hemolymph samples are stored in labeled vials with 90% ETOH and kept in dark and on ice until return to shore where they are then stored in a -40C freezer until processed.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-0928930 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=0928930
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-0929086 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=0929086
completed
Mark Butler
Old Dominion University
USA
mjbutleriv@gmail.com
pointOfContact
Donald Behringer
University of Florida
352-273-3634
UF School of Forest Resources and Conservation, Fisheries and Aquatic Sciences 7922 NW 71st Street
Gainesville
FL
32653
USA
behringer@ufl.edu
pointOfContact
Claire B. Paris-Limouzy
University of Miami Rosenstiel School of Marine and Atmospheric Science
305-421-4219
University of Miami Rosenstiel School of Marine and Atmospheric Science, Division of Marine Physics 4600 Rickenbacker Causeway
Miami
FL
33149-1098
USA
cparis@rsmas.miami.edu
pointOfContact
Jeffery Shields
Virginia Institute of Marine Science
(804) 684-7128
Virginia Institute of Marine Science
Gloucester Point
VA
23062
jeff@vims.edu
pointOfContact
asNeeded
Dataset Version: 2015-04-13
Unknown
year
date
site
lat
lon
sex
length_carap
molt_cond
leg_old_new
ant_old_new
other_old_new
visible_disease_flag
blood_sample
PCR_result
comments
pCR
theme
None, User defined
year
date
site
latitude
longitude
sex
length
No BCO-DMO term
comments
featureType
BCO-DMO Standard Parameters
Thermal Cycler
instrument
BCO-DMO Standard Instruments
Butler_FloridaBay
service
Deployment Activity
south-central Florida Bay
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Connectivity of disease in marine Ecosystems: multi-scale dynamics of a viral disease infecting caribbean spiny lobster
https://osprey.bco-dmo.org/project/472649
Connectivity of disease in marine Ecosystems: multi-scale dynamics of a viral disease infecting caribbean spiny lobster
<p>Scientists are struck by how different terrestrial epidemiology is from that in marine ecosystems, a crucial difference being the more rapid spread of diseases in the ocean due to the presumed absence of barriers to waterborne dispersal. Yet, the movement of pathogens in the sea and its importance to disease dynamics in marine metapopulations is virtually unstudied. Marine pathogens do spread among distant host populations, as demonstrated by dramatic epizootics, but is this common or demographically relevant? Nearly all studies of marine diseases treat such events as transitory, focusing instead on local disease dynamics. This approach suggests either that small-scale phenomena normally trump the influence of large-scale pathogen connectivity or, alternatively, that the dispersal of marine pathogens by highly motile adults or free-living waterborne pathogens is simply too intractable for empirical investigation. Yet, there is another perhaps unappreciated mechanism – dispersal by infected larvae. Most marine animals have life histories that include planktonic larvae, many of which are highly dispersive. If infected by pathogens, these “larval vectors” would provide an efficient mechanism for distributing pathogens at high concentrations directly into habitats where hosts dwell. More so than passive, waterborne pathogens that are subject to rapid dilution and have no means of targeting distant hosts.</p>
<p>We have evidence that long-distance pathogen dispersal in the sea via infected meroplanktonic larvae may be possible. The pathogen in question is an often lethal, pathogenic virus (PaV1; Panulirus argus virus 1) that infects the Caribbean spiny lobster, Panulirus argus – a species broadly distributed throughout the Caribbean where it supports the most valuable fishery in the region. We described the PaV1 virus in 1999 and since then have studied its pathology, epidemiology, transmission, and effects on juvenile lobster populations in the Florida Keys. Like others, our focus has been on local pathogen-host dynamics, but PaV1 infections in lobsters are now confirmed in distant areas of the Caribbean (Belize, Mexico, St. Croix) in regions that are demographically linked only by dispersing larvae that spend >6 mos. in the open ocean. We recently discovered that many lobster postlarvae recruiting to coastal nurseries in Florida are infected with PaV1, providing novel evidence for pathogen connectivity among distant host populations.</p>
<p>Focusing on the spiny lobster-PaV1 virus association as a case study, we propose an ambitious program of laboratory, field, and modeling research whose broader implications will better our understanding of the importance of dispersal by infectious agents on the spread and maintenance of disease in marine populations. The project builds upon data and techniques developed with prior NSF sponsorship, and brings together partners in developing Caribbean nations with a multidisciplinary group of scientists with long-standing research programs in larval biology, biophysical and ecological modeling, crustacean biology, molecular biology, and the study of marine diseases.</p>
Lobster disease connectivity
largerWorkCitation
project
eng; USA
biota
oceans
south-central Florida Bay
2015-04-13
Florida Keys, USA and Carrbbean
0
BCO-DMO catalogue of parameters from Data describing Long-term PaV1 lobster surveys in the south-central Florida Bay from 1999-2014 (Lobster disease connectivity project)
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/556351.rdf
Name: year
Units: yyyy
Description: <p>year of survey</p>
http://lod.bco-dmo.org/id/dataset-parameter/556352.rdf
Name: date
Units: yyyy-mm-dd
Description: <p>date of survey</p>
http://lod.bco-dmo.org/id/dataset-parameter/556353.rdf
Name: site
Units: unitless
Description: <p>survey site</p>
http://lod.bco-dmo.org/id/dataset-parameter/556354.rdf
Name: lat
Units: decimal degrees
Description: <p>latitude; north is positive</p>
http://lod.bco-dmo.org/id/dataset-parameter/556355.rdf
Name: lon
Units: decimal degrees
Description: <p>longitude; east is positive</p>
http://lod.bco-dmo.org/id/dataset-parameter/556356.rdf
Name: sex
Units: unitless
Description: <p>sex of lobster</p>
http://lod.bco-dmo.org/id/dataset-parameter/556357.rdf
Name: length_carap
Units: millimeters
Description: <p>carapace length of tethered lobster</p>
http://lod.bco-dmo.org/id/dataset-parameter/556358.rdf
Name: molt_cond
Units: unitless
Description: <p>visual assessment of molt condition: I = intermolt; PR = premolt; PO = postmolt</p>
http://lod.bco-dmo.org/id/dataset-parameter/556359.rdf
Name: leg_old_new
Units: unitless
Description: <p>old and new injuries number of old/new leg injuries if any</p>
http://lod.bco-dmo.org/id/dataset-parameter/556360.rdf
Name: ant_old_new
Units: unitless
Description: <p>number of old/new antennae injuries if any</p>
http://lod.bco-dmo.org/id/dataset-parameter/556361.rdf
Name: other_old_new
Units: unitless
Description: <p>number of old/new other injuries if any</p>
http://lod.bco-dmo.org/id/dataset-parameter/556362.rdf
Name: visible_disease_flag
Units: unitless
Description: <p>signs of PaV1 infection in lobster obvious to naked eye; 0 = no; 1 = yes</p>
http://lod.bco-dmo.org/id/dataset-parameter/556363.rdf
Name: blood_sample
Units: unitless
Description: <p>hemolymph sample vial label if any</p>
http://lod.bco-dmo.org/id/dataset-parameter/556364.rdf
Name: PCR_result
Units: unitless
Description: <p>positive or negative PCR test for presence of PaV1 virus DNAa</p>
http://lod.bco-dmo.org/id/dataset-parameter/556365.rdf
Name: comments
Units: unitless
Description: <p>comments</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
213970
https://datadocs.bco-dmo.org/file/LAAGpLJTkn16pJ/longterm_survey.csv
longterm_survey.csv
Primary data file for dataset ID 556162
download
https://osprey.bco-dmo.org/dataset/556162/data/download
download
onLine
dataset
<p>At each permanent 25m x 25m sample location (hard-bottom habitat 1.5 – 3.0 m depth), delineated by rope border affixed to seafloor, two divers search the entire site for spiny lobsters (Panulirus argus).&nbsp; All lobsters sighted are collected in hand nets by divers and brought aboard the topside vessel where the data are collected &amp; recorded.&nbsp; Lobsters are then returned live to the original site.&nbsp; Hemolymph samples are stored in labeled vials with 90% ETOH and kept in dark and on ice until return to shore where they are then stored in a -40C freezer until processed.</p>
Specified by the Principal Investigator(s)
<p><strong>BCO-DMO Processing:</strong></p>
<p>- added conventional header with dataset name, PI name, version date, reference information<br />
- renamed parameters to BCO-DMO standard<br />
- added lat/lon<br />
- changed NA and ? to nd<br />
- replace blanks with 0 for injuries, disease, blood sample, PCR Results; replaced other blanks with nd</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
pCR
pCR
PI Supplied Instrument Name: pCR Instrument Name: Thermal Cycler Instrument Short Name:Thermal Cycler Instrument Description: A thermal cycler or "thermocycler" is a general term for a type of laboratory apparatus, commonly used for performing polymerase chain reaction (PCR), that is capable of repeatedly altering and maintaining specific temperatures for defined periods of time. The device has a thermal block with holes where tubes with the PCR reaction mixtures can be inserted. The cycler then raises and lowers the temperature of the block in discrete, pre-programmed steps. They can also be used to facilitate other temperature-sensitive reactions, including restriction enzyme digestion or rapid diagnostics.
(adapted from http://serc.carleton.edu/microbelife/research_methods/genomics/pcr.html)
Deployment: Butler_FloridaBay
Butler_FloridaBay
Lobster habitat
shoreside
Butler_FloridaBay
Mark Butler
Old Dominion University
Lobster habitat
shoreside