http://lod.bco-dmo.org/id/dataset/557179
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2015-04-28
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Time-series of nutrient measurements following addition of Prochlorococcus derived POM to seawater samples collected at Station ALOHA on R/V Kilo Moana cruise KM1110 in the North Pacific Subtropical Gyre in 2011
2015-04-28
publication
2015-04-28
revision
BCO-DMO Linked Data URI
2015-04-28
creation
http://lod.bco-dmo.org/id/dataset/557179
Angelicque E. White
Oregon State University
principalInvestigator
Adina Paytan
University of California-Santa Cruz
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: White, A. E., Paytan, A. (2015) Time-series of nutrient measurements following addition of Prochlorococcus derived POM to seawater samples collected at Station ALOHA on R/V Kilo Moana cruise KM1110 in the North Pacific Subtropical Gyre in 2011. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 28 April 2015) Version Date 2015-04-28 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/557179 [access date]
Time-series of nutrient measurements following addition of Prochlorococcus derived POM to seawater collected at Station ALOHA. Dataset Description: <p>Time-series of nutrient measurements following addition of<em> Prochlorococcus</em> derived POM to seawater collected at Station ALOHA. Data published in Figure 1 in Burkhardt et al. (2014).</p>
<p><em>Related Publications and References:</em><br />
Burkhardt, B., K. S. Watkins-Brandt, D. Defforey, A. Paytan and A. E. White. 2014. Remineralization of phytoplankton-derived organic matter by natural populations of heterotrophic bacteria. <em>Marine Chemistry </em>162. doi: <a href="http://dx.doi.org/10.1016/j.marchem.2014.03.007" target="_blank">10.1016/j.marchem.2014.03.007</a></p>
<p>See Related Datasets:<br />
<a href="http://www.bco-dmo.org/dataset/557396">Controls</a><br />
<a href="http://www.bco-dmo.org/dataset/558209">Killed Controls</a><br />
<a href="http://www.bco-dmo.org/dataset/557070">Trichodesmium</a><br />
<a href="http://www.bco-dmo.org/dataset/557137">Diatoms</a><br />
<a href="http://www.bco-dmo.org/dataset/557206">OR POM</a><br />
<a href="http://www.bco-dmo.org/dataset/557470">Tricho NMR</a><br />
<a href="http://www.bco-dmo.org/dataset/557572">Diatom NMR</a></p> Methods and Sampling: <p>All analytical and sampling methodologies are described in Burkhardt et al. (2014). However, summary of most relevant methods are included here:</p>
<p>To explore the relationship between POM source and remineralization rates and stoichiometry, the investigators conducted a suite of on-deck incubation experiments in the North Pacific Subtropical Gyre (NPSG) in March of 2011 near Station ALOHA. 20-L aliquots of seawater were collected from the 75-m depth horizon at Station ALOHA. Immediately after collection, seawater was stored in the dark in an incubator continually flushed with surface seawater for ~72 hours. Dried POM material (cultured Trichodesmium IMS 101, “TRICHO”, Prochlorococcus MED4, “PRO”, T. weissflogii, “DIATOM” and the natural POM from the Oregon coast, “OR-POM”) was added to the carboys with aged Station ALOHA seawater. Each treatment was prepared in duplicate except for the OR-POM. Concentrations of ammonium (NH4) and SRP were obtained every 5 min for roughly the first half hour following POM addition to capture any solubilization trends. This initial phase was followed by discrete sampling every 3 hours. Nutrient samples were run at OSU, NMR samples were run at the University of California, Santa Cruz.</p>
<p>Nutrients were analyzed using flow-through colorimetric methods on a Technicon Auto Analyzer II. SRP was analyzed using the phosphomolybdic acid reduction; ammonium (NH4) was measured by the indophenol blue method (Gordon et al., 1993); and nitrate + nitrite (N+N) was analyzed using the cadmium reduction method of Armstrong et al. (1967). Detection limits were 55 nmol L-1 for SRP, 22 nmol L-1 for NH4, and 8 nmol L-1 for N+N. Total dissolved P and N (TDP and TDN, respectively) were determined by the alkaline persulfate oxidation method (Valderrama, 1981) using a 1:10 oxidant to sample ratio. Dissolved organic P (DOP) and N (DON) were calculated as the difference of TDP and SRP and TDN less the sum of NH4+ + NO3- + NO2-, respectively.</p>
<p>Particulate C, N, and P content of each POM type was determined by collecting a subsample of the biomass onto combusted GFF filters, wrapping in foil, flash freezing, and storing at -80 degrees C. The filters were then thawed and dried at 60 degrees C overnight, folded into tin and silver boats, and run on a Carlo-Erba C/N Analyzer for particulate C (PC) and N (PN) content (Sharp (1974). For particulate P (PP) analyses samples were thawed and combusted at 450 degrees C for 4.5 hours, then extracted with 0.15 M HCl for 1 hour at 60 degrees C. PP was then analyzed as SRP in a 1.0 cm cell at 880 nm following Strickland and Parsons (1972).</p>
<p>Molecular characterization of PP compounds was performed using subsamples of each POM type with 31P nuclear magnetic resonance (NMR) spectral analysis as per Cade-Menun et al. (2005). Samples were freeze-dried, extracted with a 25-mL solution of 0.25M NaOH 0.05M Na2EDTA for 4h, and then centrifuged. 1-mL aliquots of the supernatant and digested residue samples were analyzed for P concentrations via inductively coupled plasma optical emission spectroscopy (ICP-OES) to determine the extracted P and fraction that was not extracted. The remaining supernatant was analyzed for 31P-NMR spectroscopy on a 600 MHz Varian Unity INOVA spectrometer equipped with a 10mm broadband probe at 20 degrees C and a 90 degrees pulse. Compounds were identified by their chemical shifts (ppm) relative to an external orthophosphoric acid standard. After standardizing the orthophosphate peak in all samples to 6 ppm, peak assignments were based on Tebby and Glonek (1991) Cade-Menun and Preston (1996) and Turner et al. (2003b,c). Peak areas were calculated by integration of spectra processed with a 5 Hz line broadening, using NUTS software (Acorn NMR Inc.) as described in Paytan et al., (2003). Finally, the relative contribution of surface-adsorbed P was assessed for remaining TRICHO and DIATOM POM samples via the oxalate rinse method described in Fu et al. (2005); not enough material remained from PRO and OR-POM for similar analyses.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-0962362 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=0962362
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-0961555 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=0961555
completed
Angelicque E. White
Oregon State University
808-956-6220
1950 East West Rd.
Honolulu
HI
USA
aewhite@hawaii.edu
pointOfContact
Adina Paytan
University of California-Santa Cruz
831-459-1437
Earth & Marine Sciences C308 1156 High St
Santa Cruz
CA
95064
USA
apaytan@ucsc.edu
pointOfContact
asNeeded
Dataset Version: 28 April 2015
Unknown
replicate
taxon
exp_type
run_time_hrs
date
time_local
run_id
PO4
NO3_NO2
silicate
NO2
NH4
ISO_DateTime_Local
Carlo-Erba C/N Analyzer
inductively coupled plasma optical emission spectroscopy (ICP-OES)
nuclear magnetic resonance (NMR)
Technicon Auto Analyzer II
theme
None, User defined
replicate
taxon
experiment type
No BCO-DMO term
date
time_local
reactive phosphorus (PO4)
nitrate plus nitrite
Silicate, Si(OH)4, silicic acid
Nitrite
Ammonium
ISO_DateTime_Local
featureType
BCO-DMO Standard Parameters
Elemental Analyzer
Inductively Coupled Plasma Mass Spectrometer
Nuclear Magnetic Resonance Spectrometers
Technicon AutoAnalyzer II
instrument
BCO-DMO Standard Instruments
KM1110
service
Deployment Activity
North Pacific Subtropical Gyre near Station ALOHA (22° 45'N, 158° 00'W)
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Taxon-Specific Variability of Organic Matter Production and Remineralization Potential
https://osprey.bco-dmo.org/project/556109
Taxon-Specific Variability of Organic Matter Production and Remineralization Potential
<p><em>Description from NSF award abstract:</em><br />
The marine phosphorus (P) cycle is characterized by tight coupling between the uptake and decomposition of dissolved inorganic P (DIP) and dissolved organic P (DOP). DIP is incorporated into a broad range of cellular compounds integral for energy storage, genetic material and cell structure. Cell death and autolysis, exudation, viral lysis and grazing all lead to the release of DOP into the environment where it can be depolymerized, hydrolyzed, reassimilated, removed by absorption onto sinking particles or accumulate in the surrounding environment. In this manner, the form and composition of P in the marine environment is largely controlled by the metabolic activity of microorganisms and is intimately linked to the cycling of carbon (C) and nitrogen (N) as particulate organic P (POP) and DOP is bound to C and N in multiple forms, including esters, phospholipids and phosphonates. Thus, a consideration of marine P cycling is most relevant when P transformations are viewed as part of the nutrient and energy flow in the oceanic water column. At the ecosystem scale, the balance of productivity and respiration in the open ocean is regulated by the availability of potentially limiting nutrients such as C, N and P. Therefore, understanding the coupling of C, N, and P cycles is central to the determination of the long-term controls of the magnitude and variability of primary production and particle export. Nonetheless, a paucity of simultaneous measures of dissolved organic carbon (DOC), dissolved organic nitrogen (DON) and DOP and a relative lack of information on production and decomposition processes have hindered progress in understanding the coupled dynamics of these pools. Recent studies of dissolved organic matter (DOM) dynamics show large departures from Redfield trajectories driven by alterations in phytoplankton species composition, the stoichiometry and chemical composition of organic matter production, differential lability of organic compounds and preferential remineralization of N and P by heterotrophic bacteria. Furthermore, there is mounting evidence of the potential liberation of greenhouse gases occurring via DOP hydrolysis.</p>
<p>In this research, the investigators will characterize the composition, lability and remineralization stoichiometry of organic P-C-N produced by ecologically significant photosynthetic genera. They will conduct a series of in situ and laboratory-based bio-assays where particulate (POM) and DOM isolated from <em>Prochlorococcus</em> and phosphonate-containing strains of <em>Trichodesmium</em> are added to natural microbial populations and incubated in the laboratory and at sea. Hypothesis driven experiments will address the following objectives:</p>
<p>(1) Determine the elemental (P-C-N) stoichiometry and biomolecular alterations (31P-nuclear magnetic resonance) occurring in response to exogenous additions of <em>Trichodesmium</em> and <em>Prochlorococcus</em> POM and DOM to natural populations of heterotrophic bacteria, estimate the labile and semi-labile fraction of organic material generated by ecologically significant genera and measure potential aerobic production of select greenhouse gases (methane and ethane).</p>
<p>(2) Initiate decomposition experiments in the NPSG at opposing phases of the seasonal cycle (summer/winter) in order to capture varying microbial assemblages having different initial metabolic status and community structure.</p>
Taxon-Specific Organic P-C-N Production
largerWorkCitation
project
eng; USA
oceans
North Pacific Subtropical Gyre near Station ALOHA (22° 45'N, 158° 00'W)
2015-04-28
0
BCO-DMO catalogue of parameters from Time-series of nutrient measurements following addition of Prochlorococcus derived POM to seawater samples collected at Station ALOHA on R/V Kilo Moana cruise KM1110 in the North Pacific Subtropical Gyre in 2011
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/557193.rdf
Name: replicate
Units: A or B
Description: <p>Replicate identifier.</p>
http://lod.bco-dmo.org/id/dataset-parameter/557194.rdf
Name: taxon
Units: text
Description: <p>Taxon name.</p>
http://lod.bco-dmo.org/id/dataset-parameter/557195.rdf
Name: exp_type
Units: text
Description: <p>Experiment type/location. (lab = measured in the lab post-cruise; sea = measured at sea)</p>
http://lod.bco-dmo.org/id/dataset-parameter/557196.rdf
Name: run_time_hrs
Units: hours
Description: <p>Experiment run-time.</p>
http://lod.bco-dmo.org/id/dataset-parameter/557197.rdf
Name: date
Units: mm/dd/yyyy
Description: <p>Month, day, and year (local time zone).</p>
http://lod.bco-dmo.org/id/dataset-parameter/557198.rdf
Name: time_local
Units: HH:MM
Description: <p>Time, in hours and minutes (local time zone); 24-hour clock. Recorded during lab measurements.</p>
http://lod.bco-dmo.org/id/dataset-parameter/557199.rdf
Name: run_id
Units: alphanumeric
Description: <p>Run identification number.</p>
http://lod.bco-dmo.org/id/dataset-parameter/557200.rdf
Name: PO4
Units: micromoles per liter (umol L-1)
Description: <p>Phosphate.</p>
http://lod.bco-dmo.org/id/dataset-parameter/557201.rdf
Name: NO3_NO2
Units: micromoles per liter (umol L-1)
Description: <p>Nitrate + nitrite.</p>
http://lod.bco-dmo.org/id/dataset-parameter/557202.rdf
Name: silicate
Units: micromoles per liter (umol L-1)
Description: <p>Silicate.</p>
http://lod.bco-dmo.org/id/dataset-parameter/557203.rdf
Name: NO2
Units: micromoles per liter (umol L-1)
Description: <p>Nitrite.</p>
http://lod.bco-dmo.org/id/dataset-parameter/557204.rdf
Name: NH4
Units: micromoles per liter (umol L-1)
Description: <p>Ammonium.</p>
http://lod.bco-dmo.org/id/dataset-parameter/557205.rdf
Name: ISO_DateTime_Local
Units: YYYY-mm-ddTHH:MM:SS.xx
Description: <p>Date and time (local time zone) formatted to the ISO 8601 standard.</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
5185
https://datadocs.bco-dmo.org/file/1VVMo2YUnVx0OP/pro.csv
pro.csv
Primary data file for dataset ID 557179
download
https://osprey.bco-dmo.org/dataset/557179/data/download
download
onLine
dataset
<p>All analytical and sampling methodologies are described in Burkhardt et al. (2014). However, summary of most relevant methods are included here:</p>
<p>To explore the relationship between POM source and remineralization rates and stoichiometry, the investigators conducted a suite of on-deck incubation experiments in the North Pacific Subtropical Gyre (NPSG) in March of 2011 near Station ALOHA. 20-L aliquots of seawater were collected from the 75-m depth horizon at Station ALOHA. Immediately after collection, seawater was stored in the dark in an incubator continually flushed with surface seawater for ~72 hours. Dried POM material (cultured Trichodesmium IMS 101, “TRICHO”, Prochlorococcus MED4, “PRO”, T. weissflogii, “DIATOM” and the natural POM from the Oregon coast, “OR-POM”) was added to the carboys with aged Station ALOHA seawater. Each treatment was prepared in duplicate except for the OR-POM. Concentrations of ammonium (NH4) and SRP were obtained every 5 min for roughly the first half hour following POM addition to capture any solubilization trends. This initial phase was followed by discrete sampling every 3 hours. Nutrient samples were run at OSU, NMR samples were run at the University of California, Santa Cruz.</p>
<p>Nutrients were analyzed using flow-through colorimetric methods on a Technicon Auto Analyzer II. SRP was analyzed using the phosphomolybdic acid reduction; ammonium (NH4) was measured by the indophenol blue method (Gordon et al., 1993); and nitrate + nitrite (N+N) was analyzed using the cadmium reduction method of Armstrong et al. (1967). Detection limits were 55 nmol L-1 for SRP, 22 nmol L-1 for NH4, and 8 nmol L-1 for N+N. Total dissolved P and N (TDP and TDN, respectively) were determined by the alkaline persulfate oxidation method (Valderrama, 1981) using a 1:10 oxidant to sample ratio. Dissolved organic P (DOP) and N (DON) were calculated as the difference of TDP and SRP and TDN less the sum of NH4+ + NO3- + NO2-, respectively.</p>
<p>Particulate C, N, and P content of each POM type was determined by collecting a subsample of the biomass onto combusted GFF filters, wrapping in foil, flash freezing, and storing at -80 degrees C. The filters were then thawed and dried at 60 degrees C overnight, folded into tin and silver boats, and run on a Carlo-Erba C/N Analyzer for particulate C (PC) and N (PN) content (Sharp (1974). For particulate P (PP) analyses samples were thawed and combusted at 450 degrees C for 4.5 hours, then extracted with 0.15 M HCl for 1 hour at 60 degrees C. PP was then analyzed as SRP in a 1.0 cm cell at 880 nm following Strickland and Parsons (1972).</p>
<p>Molecular characterization of PP compounds was performed using subsamples of each POM type with 31P nuclear magnetic resonance (NMR) spectral analysis as per Cade-Menun et al. (2005). Samples were freeze-dried, extracted with a 25-mL solution of 0.25M NaOH 0.05M Na2EDTA for 4h, and then centrifuged. 1-mL aliquots of the supernatant and digested residue samples were analyzed for P concentrations via inductively coupled plasma optical emission spectroscopy (ICP-OES) to determine the extracted P and fraction that was not extracted. The remaining supernatant was analyzed for 31P-NMR spectroscopy on a 600 MHz Varian Unity INOVA spectrometer equipped with a 10mm broadband probe at 20 degrees C and a 90 degrees pulse. Compounds were identified by their chemical shifts (ppm) relative to an external orthophosphoric acid standard. After standardizing the orthophosphate peak in all samples to 6 ppm, peak assignments were based on Tebby and Glonek (1991) Cade-Menun and Preston (1996) and Turner et al. (2003b,c). Peak areas were calculated by integration of spectra processed with a 5 Hz line broadening, using NUTS software (Acorn NMR Inc.) as described in Paytan et al., (2003). Finally, the relative contribution of surface-adsorbed P was assessed for remaining TRICHO and DIATOM POM samples via the oxalate rinse method described in Fu et al. (2005); not enough material remained from PRO and OR-POM for similar analyses.</p>
Specified by the Principal Investigator(s)
<p>All data processing is described in Burkhardt et al. (2014). In general, data processing for nutrients involved conversion of raw absorbance data to nutrient concentrations using standard curves.</p>
<p>BCO-DMO processing:<br />
- Re-formatted date and time fields; added ISO_DateTime_Local.<br />
- Replaced blanks (missing data) and 'NaN' with 'nd' to indicate 'no data'.<br />
- Modified parameter names to conform with BCO-DMO naming conventions.<br />
&nbsp;</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Carlo-Erba C/N Analyzer
Carlo-Erba C/N Analyzer
PI Supplied Instrument Name: Carlo-Erba C/N Analyzer PI Supplied Instrument Description:A Carlo-Erba C/N Analyzer was used to determine particulate C (PC) and N (PN) content. Instrument Name: Elemental Analyzer Instrument Short Name: Instrument Description: Instruments that quantify carbon, nitrogen and sometimes other elements by combusting the sample at very high temperature and assaying the resulting gaseous oxides. Usually used for samples including organic material. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB01/
inductively coupled plasma optical emission spectroscopy (ICP-OES)
inductively coupled plasma optical emission spectroscopy (ICP-OES)
PI Supplied Instrument Name: inductively coupled plasma optical emission spectroscopy (ICP-OES) PI Supplied Instrument Description:Samples were analyzed for P concentrations via inductively coupled plasma optical emission spectroscopy (ICP-OES). Instrument Name: Inductively Coupled Plasma Mass Spectrometer Instrument Short Name:ICP Mass Spec Instrument Description: An ICP Mass Spec is an instrument that passes nebulized samples into an inductively-coupled gas plasma (8-10000 K) where they are atomized and ionized. Ions of specific mass-to-charge ratios are quantified in a quadrupole mass spectrometer. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB15/
nuclear magnetic resonance (NMR)
nuclear magnetic resonance (NMR)
PI Supplied Instrument Name: nuclear magnetic resonance (NMR) PI Supplied Instrument Description:Molecular characterization of PP compounds was performed using subsamples of each POM type with 31P nuclear magnetic resonance (NMR) spectral analysis. Instrument Name: Nuclear Magnetic Resonance Spectrometers Instrument Short Name:NMR Instrument Description: Instruments that identify and quantify magnetically active chemical entities by subjecting a sample to orthogonal magnetic and electrical fields. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB18/
Technicon Auto Analyzer II
Technicon Auto Analyzer II
PI Supplied Instrument Name: Technicon Auto Analyzer II PI Supplied Instrument Description:Nutrients were analyzed using flow-through colorimetric methods on a Technicon Auto Analyzer II. Instrument Name: Technicon AutoAnalyzer II Instrument Short Name: Instrument Description: A rapid flow analyzer that may be used to measure nutrient concentrations in seawater. It is a continuous segmented flow instrument consisting of a sampler, peristaltic pump, analytical cartridge, heating bath, and colorimeter. See more information about this instrument from the manufacturer. Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0291/
Cruise: KM1110
KM1110
R/V Kilo Moana
Community Standard Description
International Council for the Exploration of the Sea
R/V Kilo Moana
vessel
KM1110
Matthew J. Church
University of Hawai'i
http://dmoserv3.bco-dmo.org/jg/serv/BCO-DMO/DIAZOTROPHS_CO2/726342.html1%7Bdir=dmoserv3.whoi.edu/jg/dir/BCO-DMO/DIAZOTROPHS_CO2/,info=dmoserv3.bco-dmo.org/jg/info/BCO-DMO/DIAZOTROPHS_CO2/CO2_experimental%7D?cruise_id_eq_km1110
Report describing KM1110
R/V Kilo Moana
Community Standard Description
International Council for the Exploration of the Sea
R/V Kilo Moana
vessel