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All analytical and sampling methodologies are described in Burkhardt et al. (2014). However, summary of most relevant methods are included here:<\/p>\n
To explore the relationship between POM source and remineralization rates and stoichiometry, the investigators conducted a suite of on-deck incubation experiments in the North Pacific Subtropical Gyre (NPSG) in March of 2011 near Station ALOHA. 20-L aliquots of seawater were collected from the 75-m depth horizon at Station ALOHA. Immediately after collection, seawater was stored in the dark in an incubator continually flushed with surface seawater for ~72 hours. Dried POM material (cultured Trichodesmium IMS 101, \u201cTRICHO\u201d, Prochlorococcus MED4, \u201cPRO\u201d, T. weissflogii, \u201cDIATOM\u201d and the natural POM from the Oregon coast, \u201cOR-POM\u201d) was added to the carboys with aged Station ALOHA seawater. Each treatment was prepared in duplicate except for the OR-POM. Concentrations of ammonium (NH4) and SRP were obtained every 5 min for roughly the first half hour following POM addition to capture any solubilization trends. This initial phase was followed by discrete sampling every 3 hours. Nutrient samples were run at OSU, NMR samples were run at the University of California, Santa Cruz.<\/p>\n
Nutrients were analyzed using flow-through colorimetric methods on a Technicon Auto Analyzer II. SRP was analyzed using the phosphomolybdic acid reduction; ammonium (NH4) was measured by the indophenol blue method (Gordon et al., 1993); and nitrate + nitrite (N+N) was analyzed using the cadmium reduction method of Armstrong et al. (1967). Detection limits were 55 nmol L-1 for SRP, 22 nmol L-1 for NH4, and 8 nmol L-1 for N+N. Total dissolved P and N (TDP and TDN, respectively) were determined by the alkaline persulfate oxidation method (Valderrama, 1981) using a 1:10 oxidant to sample ratio. Dissolved organic P (DOP) and N (DON) were calculated as the difference of TDP and SRP and TDN less the sum of NH4+ + NO3- + NO2-, respectively.<\/p>\n
Particulate C, N, and P content of each POM type was determined by collecting a subsample of the biomass onto combusted GFF filters, wrapping in foil, flash freezing, and storing at -80 degrees C. The filters were then thawed and dried at 60 degrees C overnight, folded into tin and silver boats, and run on a Carlo-Erba C\/N Analyzer for particulate C (PC) and N (PN) content (Sharp (1974). For particulate P (PP) analyses samples were thawed and combusted at 450 degrees C for 4.5 hours, then extracted with 0.15 M HCl for 1 hour at 60 degrees C. PP was then analyzed as SRP in a 1.0 cm cell at 880 nm following Strickland and Parsons (1972).<\/p>\n
Molecular characterization of PP compounds was performed using subsamples of each POM type with 31P nuclear magnetic resonance (NMR) spectral analysis as per Cade-Menun et al. (2005). Samples were freeze-dried, extracted with a 25-mL solution of 0.25M NaOH 0.05M Na2EDTA for 4h, and then centrifuged. 1-mL aliquots of the supernatant and digested residue samples were analyzed for P concentrations via inductively coupled plasma optical emission spectroscopy (ICP-OES) to determine the extracted P and fraction that was not extracted. The remaining supernatant was analyzed for 31P-NMR spectroscopy on a 600 MHz Varian Unity INOVA spectrometer equipped with a 10mm broadband probe at 20 degrees C and a 90 degrees pulse. Compounds were identified by their chemical shifts (ppm) relative to an external orthophosphoric acid standard. After standardizing the orthophosphate peak in all samples to 6 ppm, peak assignments were based on Tebby and Glonek (1991) Cade-Menun and Preston (1996) and Turner et al. (2003b,c). Peak areas were calculated by integration of spectra processed with a 5 Hz line broadening, using NUTS software (Acorn NMR Inc.) as described in Paytan et al., (2003). Finally, the relative contribution of surface-adsorbed P was assessed for remaining TRICHO and DIATOM POM samples via the oxalate rinse method described in Fu et al. (2005); not enough material remained from PRO and OR-POM for similar analyses.<\/p><\/div>","@type":"rdf:HTML"}],"http:\/\/ocean-data.org\/schema\/hasBriefDescription":[{"@value":"Time-series of nutrient measurements following addition of POM collected off the coast of Oregon to seawater collected at Station ALOHA.","@language":"en-US"}],"http:\/\/purl.org\/dc\/terms\/description":[{"@value":"
Time-series of nutrient measurements following addition of POM collected off the coast of Oregon to seawater collected at Station ALOHA. Data published in Figure 1 in Burkhardt et al. (2014).<\/p>\n
Related Publications and References:<\/em> See Related Datasets: All data processing is described in Burkhardt et al. (2014). In general, data processing for nutrients involved conversion of raw absorbance data to nutrient concentrations using standard curves.<\/p>\n BCO-DMO processing:
\nBurkhardt, B., K. S. Watkins-Brandt, D. Defforey, A. Paytan and A. E. White. 2014. Remineralization of phytoplankton-derived organic matter by natural populations of heterotrophic bacteria. Marine Chemistry <\/em>162. doi: 10.1016\/j.marchem.2014.03.007<\/a><\/p>\n
Controls<\/a>
Killed Controls<\/a>
Diatoms<\/a>
Prochlorococcus<\/a>
Trichodesmium<\/a>
Tricho NMR<\/a>
Diatom NMR<\/a><\/p><\/div>","@type":"rdf:HTML"}],"http:\/\/www.w3.org\/2000\/01\/rdf-schema#label":[{"@value":"OR POM Exp","@type":"xsd:string"}],"http:\/\/ocean-data.org\/schema\/hasProcessingDescription":[{"@value":"
\n- Re-formatted date and time fields; added ISO_DateTime_Local.
\n- Replaced blanks (missing data) and 'NaN' with 'nd' to indicate 'no data'.
\n- Modified parameter names to conform with BCO-DMO naming conventions.<\/p><\/div>","@type":"rdf:HTML"}],"http:\/\/purl.org\/dc\/terms\/identifier":[{"@value":"557206","@type":"xsd:int"}],"http:\/\/purl.org\/dc\/terms\/title":[{"@value":"OR POM Exp"}],"http:\/\/purl.org\/dc\/terms\/date":[{"@value":"2015-04-28T16:10:05-04:00","@type":"xsd:dateTime"}],"http:\/\/purl.org\/dc\/terms\/created":[{"@value":"2015-04-28T16:10:05-04:00","@type":"xsd:dateTime"}],"http:\/\/purl.org\/dc\/terms\/modified":[{"@value":"2023-07-07T16:10:26-04:00","@type":"xsd:dateTime"}],"http:\/\/rdfs.org\/ns\/void#inDataset":[{"@id":"http:\/\/www.bco-dmo.org\/"}],"http:\/\/ocean-data.org\/schema\/namedGraph":[{"@value":"urn:bcodmo:dataset:557206","@type":"xsd:token"}],"http:\/\/ocean-data.org\/schema\/osprey_page":[{"@id":"https:\/\/www.bco-dmo.org\/dataset\/557206"}],"http:\/\/ocean-data.org\/schema\/identifier":[{"@value":"_:Identifier557206"}],"http:\/\/ocean-data.org\/schema\/datasetTitle":[{"@value":"Time-series of nutrient measurements following addition of POM collected off the coast of Oregon to seawater samples collected on R\/V Kilo Moana cruise KM1110 in the North Pacific Subtropical Gyre in 2011","@language":"en-US"}],"http:\/\/ocean-data.org\/schema\/abstract":[{"@value":"","@language":"en-US"}],"http:\/\/purl.org\/dc\/terms\/rights":[{"@id":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"http:\/\/ocean-data.org\/schema\/deprecated":[{"@value":"false","@type":"xsd:boolean"}],"http:\/\/purl.org\/dc\/terms\/bibliographicCitation":[{"@value":"White, A. 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