http://lod.bco-dmo.org/id/dataset/559738
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2015-06-02
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Porewater geochemistry data for sediment samples included in global seep survey from R/V Atlantis cruise AT15-40 and Alvin dives in the Guaymas Basin in 2008 (Guaymas Basin interactions project)
2015-06-02
publication
2015-06-02
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2020-08-24
publication
https://doi.org/10.26008/1912/bco-dmo.559738.1
Andreas Teske
University of North Carolina at Chapel Hill
principalInvestigator
Barbara J. MacGregor
University of North Carolina at Chapel Hill
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Teske, A., MacGregor, B. (2020) Porewater geochemistry data for sediment samples included in global seep survey from R/V Atlantis cruise AT15-40 and Alvin dives in the Guaymas Basin in 2008 (Guaymas Basin interactions project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2015-06-02 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.559738.1 [access date]
Porewater geochemistry data for sediment samples that were included in global seep survey (Ruff et al. 2015). Dataset Description: <p>Porewater geochemistry data for sediment samples that were included in global seep survey.</p> Methods and Sampling: <p><strong>Geochemical Analyses:</strong><br />
Sulfate concentration measurements were completed shipboard; after centrifuging sediment-filled 15 ml tubes, the overlying porewater was filtered through 0.45 um filters, acidified with 50 ul of 50% HCl and bubbled with nitrogen for 4 minutes to remove sulfide. Sulfate concentrations were then measured shipboard using a 2010i Dionex Ion Chromatograph (Sunnyvale, CA, USA) through Ag+ exchange columns (Dionex) so as to remove Cl- (Martens et al., 1999).</p>
<p>For sulfide, 1 ml porewater samples were combined with 0.1M zinc acetate and concentrations were analyzed spectrophotometrically on the ship (Cline 1969).</p>
<p>For methane, sediment subsamples were added to serum bottles containing a 0.1 M sodium hydroxide solution. Headspace methane concentrations were determined onboard using standard gas chromatography using a flame ionization detector. Stable isotopic compositions of methane was measured via gas chromatography/combustion/isotope ratio mass spectrometry (GC/C/IRMS) on a Finnigan MAT 252 Isotope Ratio Mass Spectrometer.</p>
<p>To measure DIC, 2 ml of unamended porewater from each sediment horizon were injected into evacuated serum vials (30 ml) and stored upside down at -20 degrees C. At UNC, the samples were thawed, and DIC was volatilized by adding 1 ml 30% phosphoric acid to each serum vial and shaking vigorously before GC analysis (Kelley et al., 1990). Stable isotopic values and concentrations of DIC were analyzed via coupled GC (Hewlett Packard 5890) and Isotope Ratio Mass Spectrometer (Finnigan MAT 252).</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1357238 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1357238
completed
Andreas Teske
University of North Carolina at Chapel Hill
508-287-1648
Department of Marine Sciences 3117B Venable Hall, CB 3300
Chapel Hill
NC
27599
USA
teske@email.unc.edu
pointOfContact
Barbara J. MacGregor
University of North Carolina at Chapel Hill
USA
macgregb@umn.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
sample
cruise_id
date
month
day
year
lat
lon
depth_range
depth_min
depth_max
temp_approx
CH4
SO4
HS
closest_geochemistry_core
Hewlett Packard 5890
2010i Dionex Ion Chromatograph
Finnigan MAT 252 Isotope Ratio Mass Spectrometer
theme
None, User defined
sample identification
Cruise identfier
date
month of year
day of month
year
latitude
longitude
depth_range
depth_min
depth_max
temperature of sample or a generic measurement (not in-situ water temp)
methane
sulfate
sulfide
core id
featureType
BCO-DMO Standard Parameters
Gas Chromatograph
Ion Chromatograph
Isotope-ratio Mass Spectrometer
instrument
BCO-DMO Standard Instruments
AT15-40
AT15-40_Alvin_Dives
service
Deployment Activity
Guaymas Basin
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Collaborative Research: Microbial Carbon cycling and its interactions with Sulfur and Nitrogen transformations in Guaymas Basin hydrothermal sediments
https://osprey.bco-dmo.org/project/474317
Collaborative Research: Microbial Carbon cycling and its interactions with Sulfur and Nitrogen transformations in Guaymas Basin hydrothermal sediments
<p><em>Description from NSF award abstract:</em><br />
Hydrothermally active sediments in the Guaymas Basin are dominated by novel microbial communities that catalyze important biogeochemical processes in these seafloor ecosystems. This project will investigate genomic potential, physiological capabilities and biogeochemical roles of key uncultured organisms from Guaymas sediments, especially the high-temperature anaerobic methane oxidizers that occur specifically in hydrothermally active sediments (ANME-1Guaymas). The study will focus on their role in carbon transformations, but also explore their potential involvement in sulfur and nitrogen transformations. First-order research topics include quantifying anaerobic methane oxidation under high temperature,in situ concentrations of phosphorus and methane , and with alternate electron acceptors; sulfate and sulfur-dependent microbial pathways and isotopic signatures under these conditions; and nitrogen transformations in methane-oxidizing microbial communities, hydrothermal mats and sediments.</p>
<p>This integrated biogeochemical and microbiological research will explore the pathways of and environmental controls on the consumption and production of methane, other alkanes, inorganic carbon, organic acids and organic matter that fuel the Guaymas sedimentary microbial ecosystem. The hydrothermal sediments of Guaymas Basin provide a spatially compact, high-activity location for investigating novel modes of methane cycling and carbon assimilation into microbial biomass. In the case of anaerobic methane oxidation, the high temperature and pressure tolerance of Guaymas Basin methane-oxidizing microbial communities, and their potential to uncouple from the dominant electron acceptor sulfate, vastly increase the predicted subsurface habitat space and biogeochemical role for anaerobic microbial methanotrophy in global deep subsurface diagenesis. Further, microbial methane production and oxidation interlocks with syulfur and nitrogen transformations, which will be explored at the organism and process level in hydrothermal sediment microbial communities and mats of Guaymas Basin. In general, first-order research tasks (rate measurements, radiotracer incorporation studies, genomes, in situ microgradients) define the key microbial capabilities, pathways and processes that mediate chemical exchange between the subsurface hydrothermal/seeps and deep ocean waters.</p>
Guaymas Basin Interactions
largerWorkCitation
project
eng; USA
oceans
Guaymas Basin
-111.4096
-111.4087
27.0065
27.0085
2008-12-06
2008-12-13
Guaymas Basin, Gulf of California, 27.00 N, 111.00W
0
BCO-DMO catalogue of parameters from Porewater geochemistry data for sediment samples included in global seep survey from R/V Atlantis cruise AT15-40 and Alvin dives in the Guaymas Basin in 2008 (Guaymas Basin interactions project)
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/559755.rdf
Name: sample
Units: alphanumeric
Description: <p>Sample identification number.</p>
http://lod.bco-dmo.org/id/dataset-parameter/559756.rdf
Name: cruise_id
Units: alphanumeric
Description: <p>Cruise identification number.</p>
http://lod.bco-dmo.org/id/dataset-parameter/559757.rdf
Name: date
Units: mm/dd/YYYY
Description: <p>Month, day, and year of sampling.</p>
http://lod.bco-dmo.org/id/dataset-parameter/559758.rdf
Name: month
Units: mm (01 to 12)
Description: <p>2-digit month of sampling.</p>
http://lod.bco-dmo.org/id/dataset-parameter/559759.rdf
Name: day
Units: dd (01 to 31)
Description: <p>2-digit day of sampling.</p>
http://lod.bco-dmo.org/id/dataset-parameter/559760.rdf
Name: year
Units: YYYY
Description: <p>4-digit year of sampling.</p>
http://lod.bco-dmo.org/id/dataset-parameter/559761.rdf
Name: lat
Units: decimal degrees
Description: <p>Latitude in decimal degrees North.</p>
http://lod.bco-dmo.org/id/dataset-parameter/559762.rdf
Name: lon
Units: decimal degrees
Description: <p>Longitude in decimal degrees East (negative = West).</p>
http://lod.bco-dmo.org/id/dataset-parameter/559763.rdf
Name: depth_range
Units: centimeters
Description: <p>Range of depth of the sediment sample.</p>
http://lod.bco-dmo.org/id/dataset-parameter/559764.rdf
Name: depth_min
Units: centimeters
Description: <p>Minimum depth of the sediment sample.</p>
http://lod.bco-dmo.org/id/dataset-parameter/559765.rdf
Name: depth_max
Units: centimeters
Description: <p>Maxium depth of the sediment sample.</p>
http://lod.bco-dmo.org/id/dataset-parameter/559766.rdf
Name: temp_approx
Units: degrees Celsius
Description: <p>Approximate temperature.</p>
http://lod.bco-dmo.org/id/dataset-parameter/559767.rdf
Name: CH4
Units: milli-Molar (mM)
Description: <p>Methane(CH4) concentration determined in closest geochemistry core.</p>
http://lod.bco-dmo.org/id/dataset-parameter/559768.rdf
Name: SO4
Units: milli-Molar (mM)
Description: <p>Sulfate (SO4) concentration determined in closest geochemistry core.</p>
http://lod.bco-dmo.org/id/dataset-parameter/559769.rdf
Name: HS
Units: milli-Molar (mM)
Description: <p>Sulfide (HS-) concentration determined in closest geochemistry core.</p>
http://lod.bco-dmo.org/id/dataset-parameter/559770.rdf
Name: closest_geochemistry_core
Units: alphanumeric
Description: <p>Identification number of the closest geochemistry core.</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
589
https://darchive.mblwhoilibrary.org/bitstream/1912/26107/1/dataset-559738_porewater-geochemistry-ruff__v1.tsv
download
https://doi.org/10.26008/1912/bco-dmo.559738.1
download
onLine
dataset
<p><strong>Geochemical Analyses:</strong><br />
Sulfate concentration measurements were completed shipboard; after centrifuging sediment-filled 15 ml tubes, the overlying porewater was filtered through 0.45 um filters, acidified with 50 ul of 50% HCl and bubbled with nitrogen for 4 minutes to remove sulfide. Sulfate concentrations were then measured shipboard using a 2010i Dionex Ion Chromatograph (Sunnyvale, CA, USA) through Ag+ exchange columns (Dionex) so as to remove Cl- (Martens et al., 1999).</p>
<p>For sulfide, 1 ml porewater samples were combined with 0.1M zinc acetate and concentrations were analyzed spectrophotometrically on the ship (Cline 1969).</p>
<p>For methane, sediment subsamples were added to serum bottles containing a 0.1 M sodium hydroxide solution. Headspace methane concentrations were determined onboard using standard gas chromatography using a flame ionization detector. Stable isotopic compositions of methane was measured via gas chromatography/combustion/isotope ratio mass spectrometry (GC/C/IRMS) on a Finnigan MAT 252 Isotope Ratio Mass Spectrometer.</p>
<p>To measure DIC, 2 ml of unamended porewater from each sediment horizon were injected into evacuated serum vials (30 ml) and stored upside down at -20 degrees C. At UNC, the samples were thawed, and DIC was volatilized by adding 1 ml 30% phosphoric acid to each serum vial and shaking vigorously before GC analysis (Kelley et al., 1990). Stable isotopic values and concentrations of DIC were analyzed via coupled GC (Hewlett Packard 5890) and Isotope Ratio Mass Spectrometer (Finnigan MAT 252).</p>
Specified by the Principal Investigator(s)
<p>The porewater data of Guaymas Basin sediment have been tabulated in Excel sheets and were in part used to characterize Guaymas Basin samples that were included in a global census of seep microbiota (Ruff et al. 2015).</p>
<p>BCO-DMO processing:<br />
- Extracted table from Word document.<br />
- Modified format of date.<br />
- Removed trailing N and W from lat/lon values. Changed lat values to negative (to indicate West).<br />
- Created separate columns for depth_min and depth_max.<br />
- Separated columns for CH4, SO4, HS-.</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Hewlett Packard 5890
Hewlett Packard 5890
PI Supplied Instrument Name: Hewlett Packard 5890 PI Supplied Instrument Description:Stable isotopic values and concentrations of DIC were analyzed via coupled GC (Hewlett Packard 5890) and Isotope Ratio Mass Spectrometer (Finnigan MAT 252). Instrument Name: Gas Chromatograph Instrument Short Name:Gas Chromatograph Instrument Description: Instrument separating gases, volatile substances, or substances dissolved in a volatile solvent by transporting an inert gas through a column packed with a sorbent to a detector for assay. (from SeaDataNet, BODC) Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB02/
2010i Dionex Ion Chromatograph
2010i Dionex Ion Chromatograph
PI Supplied Instrument Name: 2010i Dionex Ion Chromatograph PI Supplied Instrument Description:Sulfate concentrations were measured shipboard using a 2010i Dionex Ion Chromatograph (Sunnyvale, CA, USA) through Ag+ exchange columns (Dionex). Instrument Name: Ion Chromatograph Instrument Short Name:Ion Chromatograph Instrument Description: Ion chromatography is a form of liquid chromatography that measures concentrations of ionic species by separating them based on their interaction with a resin. Ionic species separate differently depending on species type and size. Ion chromatographs are able to measure concentrations of major anions, such as fluoride, chloride, nitrate, nitrite, and sulfate, as well as major cations such as lithium, sodium, ammonium, potassium, calcium, and magnesium in the parts-per-billion (ppb) range. (from http://serc.carleton.edu/microbelife/research_methods/biogeochemical/ic.html)
Finnigan MAT 252 Isotope Ratio Mass Spectrometer
Finnigan MAT 252 Isotope Ratio Mass Spectrometer
PI Supplied Instrument Name: Finnigan MAT 252 Isotope Ratio Mass Spectrometer PI Supplied Instrument Description:Stable isotopic compositions of methane was measured via gas chromatography/ combustion/isotope ratio mass spectrometry (GC/C/IRMS) on a Finnigan MAT 252 Isotope Ratio Mass Spectrometer.
Stable isotopic values and concentrations of DIC were analyzed via coupled GC (Hewlett Packard 5890) and Isotope Ratio Mass Spectrometer (Finnigan MAT 252). Instrument Name: Isotope-ratio Mass Spectrometer Instrument Short Name:IR Mass Spec; IRMS Instrument Description: The Isotope-ratio Mass Spectrometer is a particular type of mass spectrometer used to measure the relative abundance of isotopes in a given sample (e.g. VG Prism II Isotope Ratio Mass-Spectrometer). Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB16/
Cruise: AT15-40
AT15-40
R/V Atlantis
Community Standard Description
International Council for the Exploration of the Sea
R/V Atlantis
vessel
AT15-40
Andreas Teske
University of North Carolina at Chapel Hill
http://www.marine.whoi.edu/at_synop.nsf/9452cb38d8d28f30852568cd004b8077/13f181c7f933dbac052574e4006399a9?OpenDocument
Report describing AT15-40
Deployment: AT15-40_Alvin_Dives
AT15-40_Alvin_Dives
HOV Alvin
Community Standard Description
International Council for the Exploration of the Sea
HOV Alvin
submarine-manned
AT15-40_Alvin_Dives
Andreas Teske
University of North Carolina at Chapel Hill
R/V Atlantis
Community Standard Description
International Council for the Exploration of the Sea
R/V Atlantis
vessel
HOV Alvin
Community Standard Description
International Council for the Exploration of the Sea
HOV Alvin
submarine-manned