http://lod.bco-dmo.org/id/dataset/562749
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2015-07-23
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Data describing temperature-dependent sex ratios of Menidia in Florida from 2004-2008 (Local adaptation in Menidia project)
2015-07-08
publication
2015-07-08
revision
BCO-DMO Linked Data URI
2015-07-08
creation
http://lod.bco-dmo.org/id/dataset/562749
Dr David O. Conover
Stony Brook University
principalInvestigator
Dr Paul Barber
University of California-Los Angeles
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Conover, D. O., Barber, P. (2015) Data describing temperature-dependent sex ratios of Menidia in Florida from 2004-2008 (Local adaptation in Menidia project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 2015-07-08) Version Date 2015-07-08 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/562749 [access date]
Temperature-dependent sex ratios of Menidia from Florida to PEI, 2004 through 2008. Dataset Description: <p>To determine the latitudinal pattern of sex determination, embryos from M. menidia were collected from 31 locations along the species’ range from Amelia Island, FL (30° 31.13’ N, 081° 27.78’ W) to the Magdalen Islands, Quebec, Canada (47° 23.60’ N, 061° 50.76’ W) (Table 1, See Hice et al. (2012) for detailed methods). This resulted in collection sites that averaged 60-80 km from each other for complete coverage of M. menidia’s range (Figure 1). Briefly, spawning adult M. menidia were collected with a 30m seine net, stripped spawned following the methods employed by Lagomarsino and Conover (1993) and embryos were transported to Flax Pond Laboratory in Old Field, NY or the Bluepoint Laboratory in West Sayville, NY. Larvae of mixed parentage were pooled and randomly allocated into 15 °C or 28 °C in triplicate containers prior to sex determination (Conover and Fleisher 1986). Density per container was periodically adjusted and fish were euthanized when they reached a minimum of 21-23 mm TL. Time to reach this size ranged from one to six months, depending on growth rate and temperature treatment.</p>
<p>Sex was determined macroscopically with a dissecting microscope and total lengths were recorded. Females were identified by opaque or lightly pigmented fragile ovaries, as compared to thin, translucent and flexible testes in males (Conover and Fleisher 1986). In some replicates (27%), a few individuals did not reach a size at which sex could be definitively assessed, but this accounted for a small proportion (1.2%) of all fish. An average of 228 + 36 fish were sexed from each location.</p>
<p>Sea surface temperature (SST) data was collected from the NOAA National Buoy Data Center archives for estuarine or nearshore sites within the U.S.A. and from the Oceanographic Database maintained by the Dept. of Fisheries and Oceans, Canada. Data from two to three years between 2004 and 2007 were used to calculate the average SST (to reduce noise of an anomalous year), as well as mean values for the absolute range (maximum summer temperature - minimum winter temperature), summer temperature (July through September), and 30 day winter minimum temperature (coldest 30 day period). Season length was calculated as the mean number of days that SST within the estuary was <u>&gt;</u> 12 <sup>o</sup>C, as somatic growth is approximately zero below this temperature (Conover and Heins 1987a). Data for SST was available for 19 of the 31 sites.</p>
<p>Level of TSD was calculated as the difference in sex ratios of juvenile <em>M. menidia</em> reared at feminizing (15 °C) and masculinizing (28°C) temperatures (defined as, [F/(F+M) at 15 °C]-[F/(F+M) at 28 °C], Conover and Heins 1987a). Sex ratios were calculated by weighting the sex ratio from each replicate by the number of individuals. Measurements of TSD were repeated during an additional year for two populations that experienced high mortality during the common garden rearing (Norfolk, VA and Patchogue, NY). The repeated calculations of TSD were used in the data presented here. Piecewise linear regression was applied to the latitudinal distribution of TSD to determine breakpoints in the form of sex determination. The validity of five models (1 to 5 segments) was assessed using the methods in Hice et al. (2012). Confidence limits (95th percentile) were calculated using Sigmaplot (version 10.1, Systat Software, San Jose, CA, USA).</p>
<p><strong>Related Reference:</strong></p>
<p>Lyndie A. Hice,Tara A. Duffy, Stephan B. Munch and David O. Conover (2014) Spatial scale and divergent patterns of variation in adapted traits in the ocean. Ecology Letters 15: 568-575 doi: 10.1111/j.1461-0248.2012.01769.x</p>
<p><strong>Related Datasets:</strong></p>
<p><a href="http://www.bco-dmo.org/dataset/562695" target="_blank">Temperature-dependent sex ratio of Menidia and SST data</a><br />
<a href="http://www.bco-dmo.org/dataset/562834" target="_blank">Mean daily sea surface temperature</a></p> Methods and Sampling:
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-0425830 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=0425830
completed
Dr David O. Conover
Stony Brook University
631-632-7932
NY
USA
david.conover@stonybrook.edu
pointOfContact
Dr Paul Barber
University of California-Los Angeles
310-794-5349
610 Charles E. Young Dr. South
Los Angeles
CA
90095
United States
paulbarber@ucla.edu
pointOfContact
asNeeded
Dataset Version: 2015-07-08
Unknown
temp
site
lat
lon
year
sex_ratio_rep1
count_rep1_n
sex_ratio_rep2
count_rep2_n
sex_ratio_rep3
count_rep3_n
sex_ratio_wt
dissecting microscope
theme
None, User defined
water temperature at measurement depth
site
latitude
longitude
year
No BCO-DMO term
count
featureType
BCO-DMO Standard Parameters
Microscope - Optical
instrument
BCO-DMO Standard Instruments
Conover_lab
service
Deployment Activity
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Local adaptation across latitudes: spatial scales, gene flow, and correlates of countergradient growth variation
https://osprey.bco-dmo.org/project/561008
Local adaptation across latitudes: spatial scales, gene flow, and correlates of countergradient growth variation
<p><em>Description from NSF award abstract:</em><br />
Knowledge of geographic patterns in adaptive genetic variation is crucial to species conservation, yet in marine systems our understanding of this phenomenon is virtually nil. Until recently, the belief has been that because most marine species have highly dispersive or mobile life stages, local adaptation could occur only on broad geographic scales. This view is supported by comparatively low levels of genetic variation among populations as indexed by surveys of DNA markers neutral to selection. Phenotypic variation is expected to be largely of environmental origin, especially for physiological traits that are highly sensitive to environmental conditions. Stimulated by studies of the Atlantic silverside (Menidia menidia) and other species, these long-held beliefs are rapidly changing. The silverside shows dramatic evidence of highly structured local adaptation in a suite of coevolving physiological and morphological traits tightly associated with climate change across latitudes along the east coast of North America. Yet preliminary data from molecular genetic surveys suggest extensive gene flow along the entire latitudinal range.</p>
<p>This project will address two central problems. The first concerns the micro-geography of local adaptation. How finely scaled is it? How is it influenced by gene flow? Is it closely tied to physical features of the coastline? Do multiple traits co-vary similarly along the same gradient? The second problem focuses on the evolution of growth rate, a trait that strongly displays countergradient latitudinal variation not only in the silverside but also in numerous other taxa. Recent experiments show that growth rate can evolve very rapidly in response to size-selective mortality, including genetic changes caused by fishing. This project will examine the correlates of growth rate evolution. We will study how trade-offs with growth rate influence development, behavior, morphology, and the ability of animals to defend against disease. This research integrates biogeography, physiology, quantitative and molecular genetics, behavior, immunology, and developmental biology. It draws upon natural and experimentally evolved populations, thereby allowing rigorous testing of hypotheses that would be impossible in most other marine species.</p>
<p>PLAN FOR ARCHIVING AND SHARING DATA AND SPECIMENS (from original proposal):<br />
<strong>Preserved specimens: </strong>Specimens for genetic analysis will be stored whole at .80 C with replicate fin clips stored in 95% ethanol. Each sample from the field collections, those from the common garden experiments, and those from each generation of each population in the size selective harvest experiment will be subdivided and frozen or preserved in alcohol and archived at Stony Brook University and made available to other investigators. <strong>Genetic sequence data: </strong>All unique haplotypes will be submitted to Genbank as a population data set, annotated to reflect the frequency of each haplotype with respect to locality. Data sets will also be made available on Barber's website.</p>
Local adaptation in Menidia
largerWorkCitation
project
eng; USA
biota
oceans
2015-07-08
Western N. Atlantic, N 30 31' to 47 23' (Florida to Magdalen Islands, Gulf of St. Lawrence)
0
BCO-DMO catalogue of parameters from Data describing temperature-dependent sex ratios of Menidia in Florida from 2004-2008 (Local adaptation in Menidia project)
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/562757.rdf
Name: temp
Units: degrees Celsius
Description: <p>treatment temperature: either 15 or 28 degrees</p>
http://lod.bco-dmo.org/id/dataset-parameter/562758.rdf
Name: site
Units: unitless
Description: <p>location of sampling</p>
http://lod.bco-dmo.org/id/dataset-parameter/562759.rdf
Name: lat
Units: decimal degrees
Description: <p>latitude; north is positive</p>
http://lod.bco-dmo.org/id/dataset-parameter/562760.rdf
Name: lon
Units: decimal degrees
Description: <p>longitude; east is positive</p>
http://lod.bco-dmo.org/id/dataset-parameter/562761.rdf
Name: year
Units: yyyy
Description: <p>year of Temperature-dependent Sex Determination study</p>
http://lod.bco-dmo.org/id/dataset-parameter/562762.rdf
Name: sex_ratio_rep1
Units: unitless
Description: <p>sex ratio in replicate 1</p>
http://lod.bco-dmo.org/id/dataset-parameter/562763.rdf
Name: count_rep1_n
Units: fish
Description: <p>number of fish sexed from replicate 1</p>
http://lod.bco-dmo.org/id/dataset-parameter/562764.rdf
Name: sex_ratio_rep2
Units: unitless
Description: <p>sex ratio in replicate 2</p>
http://lod.bco-dmo.org/id/dataset-parameter/562765.rdf
Name: count_rep2_n
Units: fish
Description: <p>number of fish sexed from replicate 2</p>
http://lod.bco-dmo.org/id/dataset-parameter/562766.rdf
Name: sex_ratio_rep3
Units: unitless
Description: <p>sex ratio in replicate 3</p>
http://lod.bco-dmo.org/id/dataset-parameter/562767.rdf
Name: count_rep3_n
Units: fish
Description: <p>number of fish sexed from replicate 3</p>
http://lod.bco-dmo.org/id/dataset-parameter/562768.rdf
Name: sex_ratio_wt
Units: unitless
Description: <p>weighted sex ratios at 28C and 15C. Sex ratios were then weighted by the number of fish per replicate and calculated for each temperature (28 degrees C and 15 degrees C).</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
4996
https://datadocs.bco-dmo.org/file/p99nDEviyrknvD/sex_ratios.csv
sex_ratios.csv
Primary data file for dataset ID 562749
download
https://osprey.bco-dmo.org/dataset/562749/data/download
download
onLine
dataset
<p><strong>BCO-DMO Processing:</strong><br />
- added conventional header with dataset name, PI name, version date, reference information<br />
- renamed parameters to BCO-DMO standard<br />
- added temperature and longitude columns<br />
- replaced blank cells with nd<br />
- removed special characters<br />
- replaced spaces with underscores</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
dissecting microscope
dissecting microscope
PI Supplied Instrument Name: dissecting microscope Instrument Name: Microscope - Optical Instrument Short Name: Instrument Description: Instruments that generate enlarged images of samples using the phenomena of reflection and absorption of visible light. Includes conventional and inverted instruments. Also called a "light microscope". Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB05/
Deployment: Conover_lab
Conover_lab
lab StonyBrook_Conover
laboratory
Conover_lab
Dr David O. Conover
Stony Brook University
lab StonyBrook_Conover
laboratory