Table of Contents

• Dataset Description
  • Methods & Sampling
  • Data Processing Description
• Data Files
• Parameters
• Deployments
• Project Information
• Funding

2014 NOAA Seamap Fall Groundfish Cruise Stations where approximately 20 Atlantic croaker per station were collected for this project.

Sampling and Analytical Methodology:
Trawls were conducted by the NOAA SEAMAP Fall Groundfish Survey according to a stratified random sampling design using a standard SEAMAP 40’ net. Further details about sampling protocols can be found online at: http://www.gsmfc.org/seamap-gomrs.php
At each station, up to 20 Atlantic croaker (Micropogonias undulatus) were retained for this project.

BCO-DMO Processing Notes
- Generated from original file "2014 Hypoxolith sampling sites UPLOAD.csv" contributed by Benjamin Walther
- Parameter names edited to conform to BCO-DMO naming convention found at Choosing Parameter Name
- ISO Date/Time UTC Generated
- Lat/Lon in degs, mins converted to Lat/Lon in decimal degrees

Description from NSF award abstract:
Hypoxia occurs when dissolved oxygen concentrations in aquatic habitats drop below levels required by living organisms. The increased frequency, duration and intensity of hypoxia events worldwide have led to impaired health and functioning of marine and freshwater ecosystems. Although the potential impacts of hypoxic exposure are severe, there is little known about the consequences of systemic, sub-lethal exposure to hypoxic events for populations and communities of fishes. The objective of this project is to determine whether sub-lethal exposure to hypoxia during early life stages leads to poor growth and hence increased mortality. This project will use "environmental fingerprint" methods in fish ear stones (otoliths) retrospectively to identify periods of hypoxia exposure. The project will compare consequences of hypoxia exposure in different fish species from the Gulf of Mexico, the Baltic Sea, and Lake Erie, thus examining the largest anthropogenic hypoxic regions in the world spanning freshwater, estuarine, and marine ecosystems.

This project will employ long-term, permanent markers incorporated into fish otoliths to identify life-long patterns of sub-lethal hypoxia exposure far beyond time spans currently achievable using molecular markers. This work will capitalize on patterns of geochemical proxies such as Mn/Ca and I/Ca incorporated into otoliths and analyzed using laser ablation inductively coupled plasma mass spectrometry to identify patterns of sub-lethal hypoxia exposure. The investigators will then determine whether exposure results in differential growth and survival patterns compared to non-exposed fish by tracking cohorts over time and identifying characteristics of survivors. Because this work involves multiple species in multiple hypoxic regions, it will allow cross-system comparisons among unique ecosystems. The results from this project will thus provide unprecedented insight into effects of hypoxia exposure in three major basins using novel biogeochemical proxies, thereby paving the way for a fuller understanding of the impacts of "dead zones" on coastal resources.