http://lod.bco-dmo.org/id/dataset/566965
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2015-09-14
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Sampling overview from R/V Roger Revelle cruise RR1507 in the Eastern Lau Spreading Center in 2015 (Functional microbial dynamics of vent deposits project)
2015-09-21
publication
2015-09-21
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2021-12-07
publication
https://doi.org/10.26008/1912/bco-dmo.566965.1
Anna-Louise Reysenbach
Portland State University
principalInvestigator
Jeffrey S. Seewald
Woods Hole Oceanographic Institution
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Reysenbach, A., Seewald, J. S. (2021) Sampling overview from R/V Roger Revelle cruise RR1507 in the Eastern Lau Spreading Center in 2015 (Functional microbial dynamics of vent deposits project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2015-09-21 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.566965.1 [access date]
RR1507 sampling overview. Dataset Description: <p>Log of sampling events from RR1507 cruise.</p> Methods and Sampling:
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1235432 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1235432
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1233037 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1233037
completed
Anna-Louise Reysenbach
Portland State University
503-725-3864
1719 SW 10th Ave. SRTC Room 246
Portland
OR
97201
USA
bwar@pdx.edu
pointOfContact
Jeffrey S. Seewald
Woods Hole Oceanographic Institution
508-289-2966
Mail Stop #04 Fye 142A
Woods Hole
MA
02543
USA
jseewald@whoi.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
sample
type
sub_type
moleculer
culture
chemistry
RNAlater
bact_16S_rDNA
arch_16S_rDNA
mcrA
dsrB
ANME_1
date_collected
lat
lon
ROV Jason
theme
None, User defined
sample identification
sample type
No BCO-DMO term
date
latitude
longitude
featureType
BCO-DMO Standard Parameters
ROV Jason
instrument
BCO-DMO Standard Instruments
RR1507
service
Deployment Activity
Eastern Lau Spreading Center
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Geochemical effects on the functional microbial community dynamics of hyrothermal deposits along the Eastern Lau Spreading Center
https://osprey.bco-dmo.org/project/2265
Geochemical effects on the functional microbial community dynamics of hyrothermal deposits along the Eastern Lau Spreading Center
<p>Extreme environmental gradients exist at deep-sea hydrothermal vents where high temperature, low pH and reduced fluids mix with cold oxygenated seawater. This results in a plethora of microbes taking advantage of abundantly available microniches. From small subunit (16S) rRNA gene surveys and directed enrichment culturing of vent deposits from many sites, patterns in diversity are emerging that suggest that geochemical processes, particularly those that affect fluid pH, play a fundamental role in regulating microbial diversity and community composition.</p>
<p>This is a three year study at vent fields along the Eastern Lau Spreading Center (ELSC) to investigate the relationship between vent geochemistry and microbial community dynamics. The ELSC was chosen because it provides large and systematic changes in fluid and rock geochemistry, spreading rate, magmatic/tectonic processes, and proximity to the volcanic arc over its relatively short length of 397 km. The individual vent fields therefore provide excellent natural laboratories for exploring, in depth, the factors that influence the diversity and relationships of microbial communities associated with actively forming deep-sea hydrothermal deposits. The study will be carried out at 3 geochemically different hydrothermal fields along the ELSC. The scientists will document microbial community composition and diversity associated with hydrothermal deposits from each area for comparison with data obtained in 2009 and 2005. The close proximity of these geochemically distinct vent areas within the ELSC provides an ideal opportunity to investigate the effect of vent fluid and deposit geochemistry on the structure and function of microbial communities, as well as the specific roles of individual populations, associated with active hydrothermal deposits. The investigators hypothesize that, given the extreme environmental characteristics (e.g., low fluid pH and high iron at Mariner), they will see distinct differences in the metagenomes and particularly in the metatranscriptomes among the Kilo Moana, ABE and Mariner vent fields.</p>
<p>The specific objectives are to:<br />
1) Link geochemical and microbial dynamics along the ELSC (from 2005-2013);<br />
2) Use of metagenomic and transcriptomic data to explore biogeochemical cycles that are regulating the functional roles of the microbial communities in vent fields along the ELSC; and<br />
3) Use the metagenomic information to enrich for targeted novel Thermoprotei and acidophiles. Active hydrothermal metal sulfide deposits and fluid samples will be collected from the vent fields along the ELSC.</p>
<p>Bar-coded pyrosequencing of archaeal and bacterial 16S rRNA amplicons will be obtained for over eighty samples. The geochemical environment of the chimneys will be modeled to determine in situ geochemical conditions. These values will be used in statistical analyses to explore the factors affecting the observed differences in the communities. Using the 16S rRNA gene 454 pyrotags coupled with the geochemical characterization, specific samples will be selected for metagenomic and metatranscriptomic analyses (1-3 of each per site). The molecular information will be used to target specific samples that: (i) harbor novel unclassified diversity; (ii) have vent endemic lineages; and (iii) have acidophilic relatives from terrestrial systems, for enrichment culturing (using the geochemistry to help constrain culturing conditions).</p>
Functional microbial dynamics of vent deposits
largerWorkCitation
project
eng; USA
oceans
Eastern Lau Spreading Center
-176.6086121
-176.133725
-22.2157018
-20.0531712
2015-04-26
2015-05-10
Eastern Lau Spreading Center
0
BCO-DMO catalogue of parameters from Sampling overview from R/V Roger Revelle cruise RR1507 in the Eastern Lau Spreading Center in 2015 (Functional microbial dynamics of vent deposits project)
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/567935.rdf
Name: sample
Units: dimensionless
Description: <p>Sample identification number.</p>
http://lod.bco-dmo.org/id/dataset-parameter/567936.rdf
Name: type
Units: dimensionless
Description: <p>Description of sample type.</p>
http://lod.bco-dmo.org/id/dataset-parameter/567937.rdf
Name: sub_type
Units: dimensionless
Description: <p>Sample sub-type.</p>
http://lod.bco-dmo.org/id/dataset-parameter/567938.rdf
Name: moleculer
Units: dimensionless
Description: <p>Processed for DNA? Yes (+) or No (-)</p>
http://lod.bco-dmo.org/id/dataset-parameter/567939.rdf
Name: culture
Units: dimensionless
Description: <p>Processed for culturing? Yes (+) or No (-)</p>
http://lod.bco-dmo.org/id/dataset-parameter/567940.rdf
Name: chemistry
Units: dimensionless
Description: <p>Any end-member geochemical data? Yes (+) or No (-)</p>
http://lod.bco-dmo.org/id/dataset-parameter/567941.rdf
Name: RNAlater
Units: dimensionless
Description: <p>Is sample preserved in RNALater? Yes (+) or No (-)</p>
http://lod.bco-dmo.org/id/dataset-parameter/567942.rdf
Name: bact_16S_rDNA
Units: dimensionless
Description: <p>Was bacterial 16S rDNA detected by qPCR? Yes (+) or No (-)</p>
http://lod.bco-dmo.org/id/dataset-parameter/567943.rdf
Name: arch_16S_rDNA
Units: dimensionless
Description: <p>Was archaeal 16S rDNA detected by qPCR? Yes (+) or No (-)</p>
http://lod.bco-dmo.org/id/dataset-parameter/567944.rdf
Name: mcrA
Units: dimensionless
Description: <p>Was mcrA gene detected by qPCR? Yes (+) or No (-)</p>
http://lod.bco-dmo.org/id/dataset-parameter/567945.rdf
Name: dsrB
Units: dimensionless
Description: <p>Was dsrB gene detected by qPCR? Yes (+) or No (-)</p>
http://lod.bco-dmo.org/id/dataset-parameter/567946.rdf
Name: ANME_1
Units: dimensionless
Description: <p>Was ANME_1 16S rRNA gene detected by qPCR? Yes (+) or No (-)</p>
http://lod.bco-dmo.org/id/dataset-parameter/567947.rdf
Name: date_collected
Units: YYYYmmdd
Description: <p>Year, month, day when sample was collected (UTC).</p>
http://lod.bco-dmo.org/id/dataset-parameter/567948.rdf
Name: lat
Units: decimal degrees
Description: <p>Latitude of sample collection.</p>
http://lod.bco-dmo.org/id/dataset-parameter/567949.rdf
Name: lon
Units: decimal degrees
Description: <p>Longitude of sample collection.</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
5785
https://darchive.mblwhoilibrary.org/bitstream/1912/27802/1/dataset-566965_sample-overview-rr1507__v1.tsv
download
https://doi.org/10.26008/1912/bco-dmo.566965.1
download
onLine
dataset
<p>BCO-DMO Processing:<br />
- Modified parameter names to conform with BCO-DMO naming conventions.<br />
- Replaced blanks (missing data) and N/A with 'nd' to indicate 'no data'.<br />
- Replaced commas with semi-colons in the 'chemistry' column.<br />
- Changed format of lat and lon to decimal degrees.<br />
- Changed date format to yyyymmdd.</p>
<p>&nbsp;</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
ROV Jason
ROV Jason
PI Supplied Instrument Name: ROV Jason Instrument Name: ROV Jason Instrument Short Name:ROV Jason Instrument Description: The Remotely Operated Vehicle (ROV) Jason is operated by the Deep Submergence Laboratory (DSL) at Woods Hole Oceanographic Institution (WHOI). WHOI engineers and scientists designed and built the ROV Jason to give scientists access to the seafloor that didn't require them leaving the deck of the ship. Jason is a two-body ROV system. A 10-kilometer (6-mile) fiber-optic cable delivers electrical power and commands from the ship through Medea and down to Jason, which then returns data and live video imagery. Medea serves as a shock absorber, buffering Jason from the movements of the ship, while providing lighting and a bird’s eye view of the ROV during seafloor operations. During each dive (deployment of the ROV), Jason pilots and scientists work from a control room on the ship to monitor Jason’s instruments and video while maneuvering the vehicle and optionally performing a variety of sampling activities. Jason is equipped with sonar imagers, water samplers, video and still cameras, and lighting gear. Jason’s manipulator arms collect samples of rock, sediment, or marine life and place them in the vehicle’s basket or on "elevator" platforms that float heavier loads to the surface. More information is available from the operator site at URL. https://ndsf.whoi.edu/jason/
Cruise: RR1507
RR1507
R/V Roger Revelle
Community Standard Description
International Council for the Exploration of the Sea
R/V Roger Revelle
vessel
RR1507
Anna-Louise Reysenbach
Portland State University
http://dmoserv3.whoi.edu/data_docs/RR1507/RV1507report.pdf
Report describing RR1507
R/V Roger Revelle
Community Standard Description
International Council for the Exploration of the Sea
R/V Roger Revelle
vessel