http://lod.bco-dmo.org/id/dataset/616028
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2015-10-16
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Nutrient DNA sample log from coral recovery experiments performed in Florida during 2015 (Reef coral symbioses project)
2016-02-03
publication
2016-02-03
revision
BCO-DMO Linked Data URI
2016-02-03
creation
http://lod.bco-dmo.org/id/dataset/616028
Andrew Baker
University of Miami Rosenstiel School of Marine and Atmospheric Science
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Baker, A. (2016) Nutrient DNA sample log from coral recovery experiments performed in Florida during 2015 (Reef coral symbioses project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 2016-02-03) Version Date 2016-02-03 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/616028 [access date]
Nutrient DNA sample log Dataset Description: <p>This dataset includes the coral reef sample id's and their treatments with additional iron, nitrates, phosphorous and food. Coral species used were <em>Montastraea cavernosa</em> and <em>Siderastrea siderea</em>. This experimental study is being undertaken in tanks at the University of Miami.</p>
<p>Access to the DNA sample data&nbsp;themselves will be restricted until analysis is completed, typically within 2 years of collection.</p> Methods and Sampling: <p>Coral tissue biopsies were taken from replicate, one-inch cores of <em>Montastraea cavernosa</em> as small scrapings along a polyp’s calyx wall using a razor blade. The samples were immediately placed into 300uL of 1% SDS in DNA Buffer in 1.5mL Eppendorf tubes and heated to 65°C for one hour, where they are now stable at room temperature. Small aliquots of this lysate are then removed for DNA extraction on as as-needed basis.&nbsp;</p>
<p>We retain archived lysates (consisting of SDS-lysed tissue and a small piece of skeleton) that are stable long-term (&gt;18 years) at room temperature.&nbsp;&nbsp;</p>
<p>Data for this project will ultimately include symbiont:host cell ratios for these samples using a variety of Symbiodinium clade-specific probes. In addition, data may also include the results of ITS2 genotyping using Denaturing Gradient Gel Electrophoresis (DGGE). Additional data, such as environmental data collected by dataloggers during experiments or field deployments, may also be contributed.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1358699 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1358699
completed
Andrew Baker
University of Miami Rosenstiel School of Marine and Atmospheric Science
(305) 421-4642
University of Miami Rosenstiel School of Marine and Atmospheric Science 600 Rickenbacker Cswy.
Miami
FL
33149
USA
abaker@earth.miami.edu
pointOfContact
asNeeded
Dataset Version: 2016-02-03
Unknown
group
date
treatment
tissue_sample
species_core_id
theme
None, User defined
No BCO-DMO term
date
treatment
sample identification
featureType
BCO-DMO Standard Parameters
Baker_lab_2015
service
Deployment Activity
U. Miami RSMAS lab
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
New insights into the response of reef corals to climate change using the symbiont to host cell ratio as a metric of bleaching susceptibility
https://osprey.bco-dmo.org/project/472188
New insights into the response of reef corals to climate change using the symbiont to host cell ratio as a metric of bleaching susceptibility
<p><em>Description from NSF award abstract:</em><br />
The long-term future of reef ecosystems depends on the continued persistence of corals as essential habitat builders. Environmental threats facing coral reefs globally include chronic and acute thermal stress (which can result in "coral bleaching" as a result of the expulsion of their symbiotic algae), increasing CO2, and nutrient pollution. This project will assess the independent and interactive effects of these stressors using a new molecular based technique showing that coral bleaching susceptibility depends on the symbiont:host (S:H) cell ratio in coral tissues. Corals with higher ratios (more symbionts) are predicted to be more susceptible to thermal stress, suggesting that abiotic factors that affect symbiont densities can directly influence thermal tolerance. This new metric of symbiont density is of physiological relevance and has already revealed patterns that differ from currently used metrics, thus providing new insights into the dynamics of these symbioses. This project will also continue the development and application of these methods to further our understanding of the ecology of coral-algal symbioses, while helping to elucidate the responses of these critical ecosystem engineers to complex changes in their environment. The proposed activities will help us understand how tradeoffs in the regulation of symbiotic partners respond to the environment and relate to the differential susceptibility of corals to environmental stress.</p>
<p><em>From project proposal:</em><br />
In the laboratory, we will experimentally test the effects of light, temperature, nutrients, and CO2 on the S:H cell ratio in five coral species from the Caribbean and Indo-Pacific. We will then expose these corals to thermal stress to test whether conditions which elevate the S:H ratio, such as elevated nutrients, also increase coral susceptibility to bleaching. We will also test whether conditions which decrease the ratio, such as elevated CO2, decrease bleaching susceptibility. We further hypothesize that higher S:H cell ratios result in higher levels of oxidative stress when exposed to bleaching stressors, and will test this in collaboration with a leading scientist in coral immunity. Complementing these laboratory experiments, we will also assess the degree of spatial and temporal variability in S:H ratios in the field by monitoring seasonal changes at several sites in the northern Florida Keys, and in a new educational coral nursery at the University of Miami’s field station on Broad Key. In addition, we will also apply our molecular assay to existing long-term datasets in order to further test these hypotheses in a collaborative, cost-effective way.</p>
<p>These combined activities will help us understand how tradeoffs in symbiosis are regulated in response to the environment. By identifying the links between environment, S:H cell ratios, and stress susceptibility, we will be better able to understand and predict coral bleaching and mortality in response to climate change, while addressing the critical question of why there is so much variability in bleaching susceptibility among corals in space and time. Testing the hypothesis that the S:H ratio drives bleaching susceptibility may help us properly interpret a large body of previous and ongoing work testing the interactive effects of multiple stressors on corals.</p>
<p><em>Project-related links:</em><br />
<a href="http://www.facebook.com/#!/cr2lab" target="_blank">The Coral Reef Conservation Research Lab</a><br />
<a href="http://www.rsmas.miami.edu/people/faculty-index/?p=andrew-c-baker" target="_blank">Additional information about Dr. Andrew Baker</a></p>
Reef coral symbioses
largerWorkCitation
project
eng; USA
biota
oceans
U. Miami RSMAS lab
2016-02-03
Miami, Florida
0
BCO-DMO catalogue of parameters from Nutrient DNA sample log from coral recovery experiments performed in Florida during 2015 (Reef coral symbioses project)
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/616034.rdf
Name: group
Units: unitless
Description: <p>column added by BCO-DMO to enable structuring of the data</p>
http://lod.bco-dmo.org/id/dataset-parameter/616035.rdf
Name: date
Units: yyyy-mm-dd
Description: <p>date of treatment application</p>
http://lod.bco-dmo.org/id/dataset-parameter/616036.rdf
Name: treatment
Units: unitless
Description: <p>experimental treatment: Fe = addition of Iron; N = addition of Nitrates; P = addition of phosphates</p>
http://lod.bco-dmo.org/id/dataset-parameter/616037.rdf
Name: tissue_sample
Units: unitless
Description: <p>tissue sample identification code</p>
http://lod.bco-dmo.org/id/dataset-parameter/616038.rdf
Name: species_core_id
Units: unitless
Description: <p>sample id: species code + core code + specimen number</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
69681
https://datadocs.bco-dmo.org/file/wnnxNPncvPGm8R/nuts_meta.csv
nuts_meta.csv
Primary data file for dataset ID 616028
download
https://osprey.bco-dmo.org/dataset/616028/data/download
download
onLine
dataset
<p>Coral tissue biopsies were taken from replicate, one-inch cores of <em>Montastraea cavernosa</em> as small scrapings along a polyp’s calyx wall using a razor blade. The samples were immediately placed into 300uL of 1% SDS in DNA Buffer in 1.5mL Eppendorf tubes and heated to 65°C for one hour, where they are now stable at room temperature. Small aliquots of this lysate are then removed for DNA extraction on as as-needed basis.&nbsp;</p>
<p>We retain archived lysates (consisting of SDS-lysed tissue and a small piece of skeleton) that are stable long-term (&gt;18 years) at room temperature.&nbsp;&nbsp;</p>
<p>Data for this project will ultimately include symbiont:host cell ratios for these samples using a variety of Symbiodinium clade-specific probes. In addition, data may also include the results of ITS2 genotyping using Denaturing Gradient Gel Electrophoresis (DGGE). Additional data, such as environmental data collected by dataloggers during experiments or field deployments, may also be contributed.</p>
Specified by the Principal Investigator(s)
<p><strong>BCO-DMO Processing:</strong></p>
<p>- added conventional header with dataset name, PI name, version date<br />
- renamed parameters to BCO-DMO standard<br />
- reformatted dates from m/d/yy to yyyy-mm-dd<br />
- replaced spaces with underscores</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Deployment: Baker_lab_2015
Baker_lab_2015
UM-RSMAS
laboratory
UM-RSMAS
laboratory