Proteins that were identified in biological replicate Av2 that were not identified in biological replicate Av1. (GSO: Gamma Sulfur Oxidizer)<\/em><\/p>\n
\n\u00a0<\/p>\n
\u00a0<\/p>\n
\u00a0<\/p>\n
\u00a0<\/p>\n
\u00a0<\/p>\n
\"Although fewer proteins were identified in Av2, nearly all (94%) of the proteins identified in Av2 were also identified in Av1.\u00a0 Differences in the total number of proteins identified in replicate samples may result from differences in the amount of biomass obtained during sample processing.\"<\/p>\n
DMO notes:<\/strong>
\nPut multiple FASTA entries on separate lines
\nSplit out one number in FASTA header for linking
\nLeft it sorted by Total Independent Spectra column
\nAdded linkage column
\nRemoved commas in 'consensus annotation' column (signals database to put in new column)
\nReordered columns to put KEGG last -- much longer than any other column<\/p><\/div>","@type":"rdf:HTML"}],"http:\/\/www.w3.org\/2000\/01\/rdf-schema#label":[{"@value":"Inferno vent plume proteins-Av1","@type":"xsd:string"}],"http:\/\/ocean-data.org\/schema\/hasProcessingDescription":[{"@value":"Protein identifications<\/p>\n
Tandem mass spectra were interrogated against a composite database containing deduced protein sequences from lineages identified in the CTD17 clone library and lineages that are dominant in the deep ocean (background seawater). The database contained marine GSOs Candidatus Vesicomyosocius okutanii HA, Candidatus Ruthia magnifica Cm, the SUP05 metagenome (Walsh et al 2009), and SCGC AAA001-B15 (Arctic96BD-19 draft genome); the methylotrophs Methylobacter tundripaludum SV96 and Methylomicrobium alcaliphilum; iron-oxidizing bacteria Gallionella capsiferriformans ES-2 and Sideroxydans lithotrophicus ES-1; abundant lineages in seawater Candidatus Pelagibacter ubique HTCC1062; Candidatus Pelagibacter ubique HTCC1002; Ammonia-oxidizing archaea Nitrosopumilus maritimus SCM1, an uncultured marine group II (Iverson et al 2012); an incomplete hydrothermal vent metagenome (Xie et al 2011); and common contaminants. SEQUEST (v. UW2011.01.1) was used to correlate observed tandem mass spectra to peptide sequence via theoretical tandem mass spectra from the composite database described above (Eng et al 1994, Eng et al 2008). For a detailed discussion of database considerations in community proteomics see Morris et al. (2010). SEQUEST parameters included a 3.75 Da peptide mass tolerance on MS1 spectra, specifying trypsin as the enzyme, variable oxidation modification on methionine (15.9949 Da), and static modification on Cysteine residues (57.021464 Da) resulting from alkylation.<\/p><\/div>","@type":"rdf:HTML"}],"http:\/\/purl.org\/dc\/terms\/identifier":[{"@value":"627835","@type":"xsd:int"}],"http:\/\/purl.org\/dc\/terms\/title":[{"@value":"Inferno vent plume proteins-Av1"}],"http:\/\/purl.org\/dc\/terms\/date":[{"@value":"2015-11-25T11:24:25-05:00","@type":"xsd:dateTime"}],"http:\/\/purl.org\/dc\/terms\/created":[{"@value":"2015-11-25T11:24:25-05:00","@type":"xsd:dateTime"}],"http:\/\/purl.org\/dc\/terms\/modified":[{"@value":"2023-07-07T16:10:26-04:00","@type":"xsd:dateTime"}],"http:\/\/rdfs.org\/ns\/void#inDataset":[{"@id":"http:\/\/www.bco-dmo.org\/"}],"http:\/\/ocean-data.org\/schema\/namedGraph":[{"@value":"urn:bcodmo:dataset:627835","@type":"xsd:token"}],"http:\/\/ocean-data.org\/schema\/osprey_page":[{"@id":"https:\/\/www.bco-dmo.org\/dataset\/627835"}],"http:\/\/ocean-data.org\/schema\/identifier":[{"@value":"_:Identifier627835"}],"http:\/\/ocean-data.org\/schema\/datasetTitle":[{"@value":"Proteins identified from the black smoker chimney Inferno hydrothermal vent plume meta-proteome - replicate Av1 - on the Axial seamount off the coast of Washington in 2011.","@language":"en-US"}],"http:\/\/ocean-data.org\/schema\/abstract":[{"@value":"","@language":"en-US"}],"http:\/\/purl.org\/dc\/terms\/rights":[{"@id":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"http:\/\/ocean-data.org\/schema\/deprecated":[{"@value":"false","@type":"xsd:boolean"}],"http:\/\/ocean-data.org\/schema\/temporalExtent":[{"@value":"_:temporalExtent627835"}],"http:\/\/ocean-data.org\/schema\/spatialCoverage":[{"@value":"_:spatialCoverage627835"}],"http:\/\/purl.org\/dc\/terms\/bibliographicCitation":[{"@value":"Morris, R. 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