<div><p>Coral spawn and adult fragments were collected in the Florida Keys in a shallow reef environment at 30 ft. by scuba. RNA was extracted following standard protocols from Ambion kits. Samples were QCed with Nanodrop and Bionalyzer instruments and cDNA libraries were built for EST sequencing.</p>
<p><strong>Protocols:</strong><br /><a href="http://dmoserv3.bco-dmo.org/data_docs/SymBioSys/Coral-RNA-isolation-protocol-for-small-preps-in-1.5mL-or-2mL-tubes.pdf" target="_blank">Coral RNA isolation protocol (for small prep’s in 1.5mL or 2mL tubes)</a><br /><a href="http://dmoserv3.bco-dmo.org/data_docs/SymBioSys/Coral-RNA-isolation-for-large-preps-in-15mL-Falcon-tubes.pdf" target="_blank">Coral RNA isolation (for large prep’s in 15mL Falcon tubes)</a></p></div>
cDNA Expressed Sequence Tags for the corals Acropora palmate and Orbicella faveola
<div><p>This dataset includes links to NCBI accession pages for cDNA EST's for the corals <em>Acropora palmate</em> and <em>Orbicella faveola</em> (<em>Montastraea faveolata</em>).</p>
<p><strong>Related References:</strong></p>
<p>Desalvo, MK., CR. Voolstra, S. Sunagawa, JA. Schwarz, JH. Stillman, MA. Coffroth, AM. Szmant and M. Medina (2008) Differential gene expression during thermal stress and bleaching in the Caribbean coral <em>Montastraea faveolata. </em>Molecular Ecology, doi: 10.1111/j.1365-294X.2008.03879.x</p>
<p>Desalvo, MK., S. Sunagawa, PL. Fisher, CR. Voolstra, R. Iglesias-Prieto and M. Medina (2010) Coral host transcriptomic states are correlated with Symbiodinium genotypes. Molecular Ecology, 19, 1174–1186 doi: 10.1111/j.1365-294X.2010.04534.x</p>
<p>DeSalvo MK., S Sunagawa, CR. Voolstra, M. Medina (2010) Transcriptomic responses to heat stress and bleaching in the elkhorn coral <em>Acropora palmate. </em>Mar Ecol Prog Ser, 402: 97–113, doi: 10.3354/meps08372</p>
<p>DeSalvo MK., A. Estrada, S. Sunagawa, M. Medina (2011) Transcriptomic responses to darkness stress point to common coral bleaching mechanisms. Coral Reefs, doi 10.1007/s00338-011-0833-4.</p>
<p>Schwarz JA, PB Brokstein, C Voolstra, AY Terry, DJ Miller, AM Szmant, MA Coffroth and M Medina (2008) Coral life history and symbiosis: Functional genomic resources for two reef building Caribbean corals, <em>Acropora palmata </em>and <em>Montastraea faveolata</em>. BMC Genomics, 9:97, doi:10.1186/1471-2164-9-97.</p>
<p>Voolstra CR., JA. Schwarz, J Schnetzer,S Sunagawa,MK. Desalvo, AM. Szmant, MA Coffroth and M Medina (2009) The host transcriptome remains unaltered during the establishment of coral–algal symbioses. Molecular Ecology, 18, 1823–1833, doi: 10.1111/j.1365-294X.2009.04167.x</p></div>
Coral cDNA Expressed Sequences
<div><p>Raw sequence data have been submitted to NCBI.</p>
<p><strong>BCO-DMO Processing:</strong><br />
- added conventional header with dataset name, PI name, version date, reference information<br />
- renamed parameters to BCO-DMO standard<br />
- added species column and links to NCBI accessions were added to the EST accession numbers.<br />
- split lats and lon into separate columns<br />
- replaced spaces with underscores</p></div>
627996
Coral cDNA Expressed Sequences
2015-12-02T11:13:30-05:00
2015-12-02T11:13:30-05:00
2023-07-07T16:10:26-04:00
urn:bcodmo:dataset:627996
cDNA expressed sequence tags for the corals Acropora palmate and Orbicella faveola in Mexico, Panama, and the Florida Keys in 2003 (SymBioSys project)
false
Medina, M. (2016) cDNA expressed sequence tags for the corals Acropora palmate and Orbicella faveola in Mexico, Panama, and the Florida Keys in 2003 (SymBioSys project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 2016-02-01) Version Date 2016-02-01 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/627996 [access date]
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2016-02-01
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