http://lod.bco-dmo.org/id/dataset/628993
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2015-12-16
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Results from shipboard high-pressure incubations of diffuse flow vent fluids collected from the Crab Spa and Alvinella sites at East Pacific Rise during the AT26-10 expedition, Jan. 2014 (Microbial Communities at Deep-Sea Vents project)
2017-02-07
publication
2017-02-07
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2017-02-13
publication
https://doi.org/10.1575/1912/bco-dmo.682108
Dionysis Foustoukos
Carnegie Institution for Science
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Foustoukos, D. (2017) Results from shipboard high-pressure incubations of diffuse flow vent fluids collected from the Crab Spa and Alvinella sites at East Pacific Rise during the AT26-10 expedition, Jan. 2014 (Microbial Communities at Deep-Sea Vents project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 2) Version Date 2017-02-07 [if applicable, indicate subset used]. doi:10.1575/1912/bco-dmo.682108 [access date]
Results of on-board incubations of microbes in diffuse flow vent fluids collected from Crab Spa and Alvinella patch Dataset Description: <p>This dataset includes results from shipboard high-pressure incubations of diffuse flow vent fluids collected from the Crab Spa (9.8398º N, 104.2913º W) and Alvinella (9.8398º N, 104.2915º W) sites at East Pacific Rise during the AT26-10 oceanographic expedition in January 2014. Reported parameters include dates and time elapsed, flow rate, temperature, pressure, and pH, and concentrations of NO3, NH4, H2, H2S, CH4.</p>
<p>Vent fluids used in shipboard incubations were corrected from diffuse flow vent sites at the East Pacific Rise (2503 m): Crab Spa (9.8398º N, 104.2913º W) and Alvinella (9.8398º N, 104.2915º W) (see description in McNichol et al. [2016]). Fluids were collected using isobaric gas-tight samplers [Seewald et al., 2002] prior to their transfer to the shipboard continuous culture system [Foustoukos and Perez-Rodriguez, 2015]. Here, high-pressure incubations (250 bars) were conducted at mesophilic (30 ºC) and thermophilic (50 ºC) conditions to constrain the function and metabolic rates of denitrifying and DNRA microbial communities residing at Crab Spa and Alvinella, respectively. To enhance the activity of nitrate-respiring anaerobic bacteria, an NO3- (5 mm) and H2(aq) (1.30 mM)-enriched medium was introduced in the high-pressure incubations under strictly anaerobic conditions. Dissolved HCO3- (7.3 mm, 13C labeled) was used as added carbon source. Vent fluids were introduced at a flow rate of 0.042 mL/min, while growth medium was added at a rate of 0.0042 mL/min. The two sets of experiments were performed for 356 (Crab Spa) and 50 hours (Alvinella). Direct cell counts were conducted by staining cells with 0.1% acridine orange and counting them with a fluorescence microscope. 15N/14N isotopic analysis of the NO3-, NH4+ and biomass were conducted with a Thermo Scientific Delta VPlus mass spectrometer and CE Instruments NA 2500 series elemental analyzer (EA).</p>
<p><strong>References:</strong></p>
<p>Foustoukos, D., and I. Perez-Rodriguez (2015), A continuous culture system for assessing microbial activities in the piezosphere, Applied and Environmental Microbiology, 81(19), 6850-6856.</p>
<p>McNichol, J., S. P. Sylva, F. Thomas, C. D. Taylor, S. M. Sievert, and J. S. Seewald (2016), Assessing microbial processes in deep-sea hydrothermal systems by incubation at in situ temperature and pressure, Deep Sea Research Part I: Oceanographic Research Papers, 115, 221-232.</p>
<p>Seewald, J. S., K. W. Doherty, T. R. Hammar, and S. P. Liberatore (2002), A new gas-tight isobaric sampler for hydrothermal fluids, Deep-Sea Research, Part I: Oceanographic Research Papers, 49(1), 189-196.</p> Methods and Sampling: <p>From AT26-10&nbsp;cruise report (01/29/2014):<br />
<strong>DOB: An Integrated Study of Energy Metabolism, Carbon Fixation, and Colonization Mechanisms in Chemosynthetic Microbial Communities at Deep-Sea Vents</strong><br />
Cruise Report by the CIW research team: Dr. Ileana Perez-Rodriguez, Mr. Matt Rawls and Dr. Dionysis I. Foustoukos<br />
The CIW team was responsible for the shipboard continuous culturing incubations of vent fluids collected from Crab Spa and Tica hot springs during the AT26-10 expedition at 9oN EPR by utilizing our high-pressure bioreactor (Fig. 1). This was accomplished through a collaborative effort with Jeff Seewald and Sean Sylva (WHOI), who deployed isobaric gas-tight samplers (IGTs) to collect hydrothermal vent fluids at the diffuse flow sites. Experiments were designed to study the cycling to N through the metabolic processes of denitrification and dissimilatory nitrate reduction to ammonia (DNRA) under in-situ deep-sea vent temperature and pressure conditions.</p>
<p>We studied the evolution of nitrate reducing microorganisms at mesophilic (30oC) and thermophilic (50oC) conditions at pressures ranging from 5 to 250 bar. Vent fluids (16 IGTs) were delivered in the bioreactor and homogeneously mixed with aqueous media solution enriched in dissolved nitrate, hydrogen and 13C labeled bicarbonate to facilitate the growth of nitrate reducing microorganisms (Fig. 2). The two distinct sets of experiments were lasted for 356 and 100 hours. In short, experimental results constrained the function and metabolic rates of the denitrifying microbial communities in the Crab Spa fluids, while DNRA metabolic pathways were identified for the populations residing in the moderate temperature vent fluids (60oC) of the Alvinella colony at Tica.</p>
<p>During the course of the experiments we monitored the growth of deep-sea microbial communities by measuring the concentrations of dissolved aqueous species directly involved in nitrate based metabolism, such as NO3, NH4, H2 and H2S. We also monitored cell densities by utilizing an epi-fluorescence microscope (Sievert, WHOI). Dissolved gas and NH4+ concentrations were attained by gas and ion chromatography (Seewald - Sylva, WHOI). Subsamples were also collected for a number of offshore analysis to determine: i) the 15N/14N isotope composition of NO3-,/NH4+ and constrain kinetic isotope effects associated with denitrification/DNRA (Perez-Rodriguez, CIW), ii) to study the rates of autotrophic carbon fixation by NanoSIMS (Musat, UFZ), iii) to perform single cell genomics on the microbial populations grown in the bioreactor (Ramunas, Bigelow) and (iv) to isolate and characterize novel microogranisms from the communities cultured in our experiments (Perez-Rodriguez, CIW and Vetriani, Rutgers).</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1136608 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1136608
completed
Dionysis Foustoukos
Carnegie Institution for Science
1-202-478-8968
Geophysical Laboratory 5251 Broad Branch Rd. NW
Washington
DC
20015
USA
dfoustoukos@cis.edu
pointOfContact
asNeeded
Dataset Version: 2
Unknown
description
date_start
date_end
flow_rate
temp
press
time_elapsed
NO3_uM
NH4_uM
H2_uM
H2S_uM
CH4_uM
pH
cell_concentration
d15N_NO3_ppt
d15N_Biomass_ppt
IGT Sampler
custom high pressure bioreactor
theme
None, User defined
experiment id
date_start
date end
No BCO-DMO term
water temperature at measurement depth
water pressure
time_elapsed
Nitrate
Ammonium
sulfide
pH
cell_concentration
d15N
featureType
BCO-DMO Standard Parameters
Electron Microscope
Isobaric Gas-Tight Sampler
Microscope - Optical
Shipboard Incubator
instrument
BCO-DMO Standard Instruments
AT26-10
service
Deployment Activity
East Pacific Rise, Pacific Ocean
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Dimensions of Biodiversity
http://www.nsf.gov/funding/pgm_summ.jsp?pims_id=503446
Dimensions of Biodiversity
(adapted from the NSF Synopsis of Program)
Dimensions of Biodiversity is a program solicitation from the NSF Directorate for Biological Sciences. FY 2010 was year one of the program. [MORE from NSF]
The NSF Dimensions of Biodiversity program seeks to characterize biodiversity on Earth by using integrative, innovative approaches to fill rapidly the most substantial gaps in our understanding. The program will take a broad view of biodiversity, and in its initial phase will focus on the integration of genetic, taxonomic, and functional dimensions of biodiversity. Project investigators are encouraged to integrate these three dimensions to understand the interactions and feedbacks among them. While this focus complements several core NSF programs, it differs by requiring that multiple dimensions of biodiversity be addressed simultaneously, to understand the roles of biodiversity in critical ecological and evolutionary processes.
Dimensions of Biodiversity
largerWorkCitation
program
Center for Dark Energy Biosphere Investigations
http://www.darkenergybiosphere.org
Center for Dark Energy Biosphere Investigations
The mission of the Center for Dark Energy Biosphere Investigations (C-DEBI) is to explore life beneath the seafloor and make transformative discoveries that advance science, benefit society, and inspire people of all ages and origins.
C-DEBI provides a framework for a large, multi-disciplinary group of scientists to pursue fundamental questions about life deep in the sub-surface environment of Earth. The fundamental science questions of C-DEBI involve exploration and discovery, uncovering the processes that constrain the sub-surface biosphere below the oceans, and implications to the Earth system. What type of life exists in this deep biosphere, how much, and how is it distributed and dispersed? What are the physical-chemical conditions that promote or limit life? What are the important oxidation-reduction processes and are they unique or important to humankind? How does this biosphere influence global energy and material cycles, particularly the carbon cycle? Finally, can we discern how such life evolved in geological settings beneath the ocean floor, and how this might relate to ideas about the origin of life on our planet?
C-DEBI's scientific goals are pursued with a combination of approaches:
(1) coordinate, integrate, support, and extend the research associated with four major programs—Juan de Fuca Ridge flank (JdF), South Pacific Gyre (SPG), North Pond (NP), and Dorado Outcrop (DO)—and other field sites;
(2) make substantial investments of resources to support field, laboratory, analytical, and modeling studies of the deep subseafloor ecosystems;
(3) facilitate and encourage synthesis and thematic understanding of submarine microbiological processes, through funding of scientific and technical activities, coordination and hosting of meetings and workshops, and support of (mostly junior) researchers and graduate students; and
(4) entrain, educate, inspire, and mentor an interdisciplinary community of researchers and educators, with an emphasis on undergraduate and graduate students and early-career scientists.
Note: Katrina Edwards was a former PI of C-DEBI; James Cowen is a former co-PI.
Data Management:
C-DEBI is committed to ensuring all the data generated are publically available and deposited in a data repository for long-term storage as stated in their Data Management Plan (PDF) and in compliance with the NSF Ocean Sciences Sample and Data Policy. The data types and products resulting from C-DEBI-supported research include a wide variety of geophysical, geological, geochemical, and biological information, in addition to education and outreach materials, technical documents, and samples. All data and information generated by C-DEBI-supported research projects are required to be made publically available either following publication of research results or within two (2) years of data generation.
To ensure preservation and dissemination of the diverse data-types generated, C-DEBI researchers are working with BCO-DMO Data Managers make data publicly available online. The partnership with BCO-DMO helps ensure that the C-DEBI data are discoverable and available for reuse. Some C-DEBI data is better served by specialized repositories (NCBI's GenBank for sequence data, for example) and, in those cases, BCO-DMO provides dataset documentation (metadata) that includes links to those external repositories.
C-DEBI
largerWorkCitation
program
An Integrated Study of Energy Metabolism, Carbon Fixation, and Colonization Mechanisms in Chemosynthetic Microbial Communities at Deep-Sea Vents
https://osprey.bco-dmo.org/project/2216
An Integrated Study of Energy Metabolism, Carbon Fixation, and Colonization Mechanisms in Chemosynthetic Microbial Communities at Deep-Sea Vents
<p></p><p>
Deep-sea hydrothermal vents, first discovered in 1977, are poster child ecosystems where microbial chemosynthesis rather than photosynthesis is the primary source of organic carbon. Significant gaps remain in our understanding of the underlying microbiology and biogeochemistry of these fascinating ecosystems. Missing are the identification of specific microorganisms mediating critical reactions in various geothermal systems, metabolic pathways used by the microbes, rates of the catalyzed reactions, amounts of organic carbon being produced, and the larger role of these ecosystems in global biogeochemical cycles. To fill these gaps, the investigators will conduct a 3-year interdisciplinary, international hypothesis-driven research program to understand microbial processes and their quantitative importance at deep-sea vents. Specifically, the investigators will address the following objectives: 1. Determine key relationships between the taxonomic, genetic and functional diversity, as well as the mechanisms of energy and carbon transfer, in deep-sea hydrothermal vent microbial communities. 2. Identify the predominant metabolic pathways and thus the main energy sources driving chemoautotrophic production in high and low temperature diffuse flow vents. 3. Determine energy conservation efficiency and rates of aerobic and anaerobic chemosynthetic primary productivity in high and low temperature diffuse flow vents. 4. Determine gene expression patterns in diffuse-flow vent microbial communities during attachment to substrates and the development of biofilms.
</p>
<p>
Integration: To address these objectives and to characterize the complexity of microbially-catalyzed processes at deep-sea vents at a qualitatively new level, we will pursue an integrated approach that couples an assessment of taxonomic diversity using cultivation-dependent and -independent approaches with methodologies that address genetic diversity, including a) metagenomics (genetic potential and diversity of community), b) single cell genomics (genetic potential and diversity of uncultured single cells), c) meta-transcriptomics and -proteomics (identification and function of active community members, realized potential of the community). To assess function and response to the environment, these approaches will be combined with 1) measurement of in situ rates of chemoautotrophic production, 2) geochemical characterization of microbial habitats, and 3) shipboard incubations under simulated in situ conditions (hypothesis testing under controlled physicochemical conditions). Network approaches and mathematical simulation will be used to reconstruct the metabolic network of the natural communities. A 3-day long project meeting towards the end of the second year will take place in Woods Hole. This Data Integration and Synthesis meeting will allow for progress reports and presentations from each PI, postdoc, and/or student, with the aim of synthesizing data generated to facilitate the preparation of manuscripts.
</p>
<p>
Intellectual Merit. Combining the community expression profile with diversity and metagenomic analyses as well as process and habitat characterization will be unique to hydrothermal vent microbiology. The approach will provide new insights into the functioning of deep-sea vent microbial communities and the constraints regulating the interactions between the microbes and their abiotic and biotic environment, ultimately enabling us to put these systems into a quantitative framework and thus a larger global context.
</p>
<p>
Broader Impacts. This is an interdisciplinary and collaborative effort between 4 US and 4 foreign institutions, creating unique opportunities for networking and fostering international collaborations. This will also benefit the involved students (2 graduate, several undergraduate) and 2 postdoctoral associates. This project will directly contribute to many educational and public outreach activities of the involved PIs, including the WHOI Dive & Discover program; single cell genomics workshops and Cafe Scientifique (Bigelow); REU (WHOI, Bigelow, CIW); COSEE and RIOS (Rutgers), and others. The proposed research fits with the focus of a number of multidisciplinary and international initiatives, in which PIs are active members (SCOR working group on Hydrothermal energy and the ocean carbon cycle, <a href="http://www.scorint">http://www.scorint</a>. org/Working_Groups/wg135.htm; Deep Carbon Observatory at CIW, <a href="https://dco.gl.ciw.edu/;">https://dco.gl.ciw.edu/;</a> Global Biogeochemical Flux (GBF) component of the Ocean Observatories Initiative (OOI), <a href="http://www.whoi.edu/GBF-OOI/page.do?pid=41475">http://www.whoi.edu/GBF-OOI/page.do?pid=41475</a>)
</p>
Microbial Communities at Deep-Sea Vents
largerWorkCitation
project
eng; USA
biota
oceans
East Pacific Rise, Pacific Ocean
-104.2915
-104.2915
9.83992
9.83992
2014-01-03
2014-01-22
0
BCO-DMO catalogue of parameters from Results from shipboard high-pressure incubations of diffuse flow vent fluids collected from the Crab Spa and Alvinella sites at East Pacific Rise during the AT26-10 expedition, Jan. 2014 (Microbial Communities at Deep-Sea Vents project)
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/629031.rdf
Name: description
Units: unitless
Description: <p>description of experimental incubation</p>
http://lod.bco-dmo.org/id/dataset-parameter/629032.rdf
Name: date_start
Units: unitless
Description: <p>start date of incubation in yyyy-mm-dd format</p>
http://lod.bco-dmo.org/id/dataset-parameter/629033.rdf
Name: date_end
Units: unitless
Description: <p>end date of incubation in yyyy-mm-dd format</p>
http://lod.bco-dmo.org/id/dataset-parameter/629034.rdf
Name: flow_rate
Units: milliliters/minute
Description: <p>flow rate</p>
http://lod.bco-dmo.org/id/dataset-parameter/629035.rdf
Name: temp
Units: degrees Celsius
Description: <p>temperature</p>
http://lod.bco-dmo.org/id/dataset-parameter/629036.rdf
Name: press
Units: MegaPascals
Description: <p>pressure</p>
http://lod.bco-dmo.org/id/dataset-parameter/629037.rdf
Name: time_elapsed
Units: hours
Description: <p>time since start of incubation</p>
http://lod.bco-dmo.org/id/dataset-parameter/629038.rdf
Name: NO3_uM
Units: umoles/kgr
Description: <p>nitrate concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/629039.rdf
Name: NH4_uM
Units: umoles/kgr
Description: <p>ammonium concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/629040.rdf
Name: H2_uM
Units: umoles/kgr
Description: <p>hydrogen concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/629041.rdf
Name: H2S_uM
Units: umoles/kgr
Description: <p>hydrogen sulfide concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/629042.rdf
Name: CH4_uM
Units: umoles/kgr
Description: <p>methane concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/629043.rdf
Name: pH
Units: unitless
Description: <p>pH at 25 C</p>
http://lod.bco-dmo.org/id/dataset-parameter/764198.rdf
Name: cell_concentration
Units: unknown
Description: <p>cell_concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/764199.rdf
Name: d15N_NO3_ppt
Units: unknown
Description: <p>d15N_NO3_ppt</p>
http://lod.bco-dmo.org/id/dataset-parameter/764200.rdf
Name: d15N_Biomass_ppt
Units: unknown
Description: <p>d15N_Biomass_ppt</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
5154
https://darchive.mblwhoilibrary.org/bitstream/1912/8706/1/data_incubation-diffuse-flow-vent-fluids-crab-spa.tsv
download
https://doi.org/10.1575/1912/bco-dmo.682108
download
onLine
dataset
<p>From AT26-10&nbsp;cruise report (01/29/2014):<br />
<strong>DOB: An Integrated Study of Energy Metabolism, Carbon Fixation, and Colonization Mechanisms in Chemosynthetic Microbial Communities at Deep-Sea Vents</strong><br />
Cruise Report by the CIW research team: Dr. Ileana Perez-Rodriguez, Mr. Matt Rawls and Dr. Dionysis I. Foustoukos<br />
The CIW team was responsible for the shipboard continuous culturing incubations of vent fluids collected from Crab Spa and Tica hot springs during the AT26-10 expedition at 9oN EPR by utilizing our high-pressure bioreactor (Fig. 1). This was accomplished through a collaborative effort with Jeff Seewald and Sean Sylva (WHOI), who deployed isobaric gas-tight samplers (IGTs) to collect hydrothermal vent fluids at the diffuse flow sites. Experiments were designed to study the cycling to N through the metabolic processes of denitrification and dissimilatory nitrate reduction to ammonia (DNRA) under in-situ deep-sea vent temperature and pressure conditions.</p>
<p>We studied the evolution of nitrate reducing microorganisms at mesophilic (30oC) and thermophilic (50oC) conditions at pressures ranging from 5 to 250 bar. Vent fluids (16 IGTs) were delivered in the bioreactor and homogeneously mixed with aqueous media solution enriched in dissolved nitrate, hydrogen and 13C labeled bicarbonate to facilitate the growth of nitrate reducing microorganisms (Fig. 2). The two distinct sets of experiments were lasted for 356 and 100 hours. In short, experimental results constrained the function and metabolic rates of the denitrifying microbial communities in the Crab Spa fluids, while DNRA metabolic pathways were identified for the populations residing in the moderate temperature vent fluids (60oC) of the Alvinella colony at Tica.</p>
<p>During the course of the experiments we monitored the growth of deep-sea microbial communities by measuring the concentrations of dissolved aqueous species directly involved in nitrate based metabolism, such as NO3, NH4, H2 and H2S. We also monitored cell densities by utilizing an epi-fluorescence microscope (Sievert, WHOI). Dissolved gas and NH4+ concentrations were attained by gas and ion chromatography (Seewald - Sylva, WHOI). Subsamples were also collected for a number of offshore analysis to determine: i) the 15N/14N isotope composition of NO3-,/NH4+ and constrain kinetic isotope effects associated with denitrification/DNRA (Perez-Rodriguez, CIW), ii) to study the rates of autotrophic carbon fixation by NanoSIMS (Musat, UFZ), iii) to perform single cell genomics on the microbial populations grown in the bioreactor (Ramunas, Bigelow) and (iv) to isolate and characterize novel microogranisms from the communities cultured in our experiments (Perez-Rodriguez, CIW and Vetriani, Rutgers).</p>
Specified by the Principal Investigator(s)
<p><strong>BCO-DMO Processing:</strong></p>
<ul>
<li>added conventional header with dataset name, PI name, version date</li>
<li>renamed parameters to BCO-DMO standard</li>
<li>concatenated the 2 datasets: Crab Spa and Alvinella patch</li>
<li>blank cells replaced with 'nd'</li>
<li>added columns for description, date_start and date_end</li>
<li>version&nbsp;2017-02-07 replaced version 2015-12-17: added cell concentration, d15N_NO3_ppt, and d15N_Biomass_ppt</li>
</ul>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
PI Supplied Instrument Name: PI Supplied Instrument Description:JSM-6500F field emission scanning electron microscope (JEOL) Instrument Name: Electron Microscope Instrument Short Name: Instrument Description: Instruments that generate enlarged images of samples using the phenomena of reflection and absorption of electrons behaving as waves. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB07/
IGT Sampler
IGT Sampler
PI Supplied Instrument Name: IGT Sampler Instrument Name: Isobaric Gas-Tight Sampler Instrument Short Name:IGT Sampler Instrument Description: Isobaric Gas Tight (IGT) samplers, designed and built by scientists and engineers at WHOI, are titanium instruments designed to be used with deep submergence vehicles to sample corrosive hydrothermal vent fluids at high temperature and high pressure. The IGT prevents the sampled fluid from degassing as pressure decreases during the vehicle’s ascent to the surface.
PI Supplied Instrument Name: PI Supplied Instrument Description:Olympus BX61 microscope with a UPlanF1 100x (numerical aperture, 1.3) oil immersion objective Instrument Name: Microscope - Optical Instrument Short Name: Instrument Description: Instruments that generate enlarged images of samples using the phenomena of reflection and absorption of visible light. Includes conventional and inverted instruments. Also called a "light microscope". Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB05/
custom high pressure bioreactor
custom high pressure bioreactor
PI Supplied Instrument Name: custom high pressure bioreactor PI Supplied Instrument Description:The integrated system allows for the culturing of microorganisms under hydrostatic pressures from 0.1 to 69 MPa (and up to 138 MPa with ongoing developments) and at temperatures ranging from 25 to 120°C. For full description, see Foustoukos and Perez-Rodriguez (2015), Applied and Environmental Microbiology, 81, 6850 Instrument Name: Shipboard Incubator Instrument Short Name: Instrument Description: A device mounted on a ship that holds water samples under conditions of controlled temperature or controlled temperature and illumination.
Cruise: AT26-10
AT26-10
R/V Atlantis
Community Standard Description
International Council for the Exploration of the Sea
R/V Atlantis
vessel
AT26-10
Stefan M. Sievert
Woods Hole Oceanographic Institution
http://dmoserv3.bco-dmo.org/data_docs/Microbe_Vent_Communities/AT26-10_Cruise_Report_v2_2015-07-09.pdf
Report describing AT26-10
R/V Atlantis
Community Standard Description
International Council for the Exploration of the Sea
R/V Atlantis
vessel