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            <gco:CharacterString>Cite this dataset as: Girguis, P., Glazer, B., Huber, J. (2022) Dissolved organic carbon and total dissolved nitrogen from the formation fluids recovered from the CORKs installed at the North Pond; collected on Maria S. Merian cruise MSM20-5 in 2012. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2015-12-30 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.630362.1 [access date]</gco:CharacterString>
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        <gco:CharacterString>Dissolved organic carbon and total dissolved nitrogen from the formation fluids recovered from the CORKs installed at the North Pond in 2012. Dataset Description: &amp;lt;p&amp;gt;Dissolved organic carbon&amp;amp;nbsp;and total dissolved nitrogen from&amp;amp;nbsp;the formation fluids recovered from the CORKs installed at the North Pond in 2012&amp;amp;nbsp;on MSM20-5. The North Pond is an isolated, northeast-trending, ~8 km × 15 km sediment pond located on the western flank of the Mid-Atlantic Ridge (MAR) at 22°45' N and 46°05' W.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Details of these CORKS and their positions, construction and depth can be found in the Proceedings of the IODP expedition 336. See:&amp;amp;nbsp;&amp;lt;a href=&amp;quot;http://publications.iodp.org/scientific_prospectus/336/336sp_6.htm&amp;quot; target=&amp;quot;_blank&amp;quot;&amp;gt;http://publications.iodp.org/scientific_prospectus/336/336sp_6.htm&amp;lt;/a&amp;gt;&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;In addition to NSF OCE-1061934 (to Girguis), this dataset was funded by C-DEBI (OCE-0939564) sub-award number 41940192 granted to Beate Kraft.&amp;lt;/p&amp;gt; Methods and Sampling: &amp;lt;p&amp;gt;Dissolved organic carbon (DOC) and total dissolved nitrogen (TDN) were measured by high temperature (680 ºC) combustion using a Shimadzu TOC-L analyzer at the SOEST Laboratory for Analytical Biogeochemistry, University of Hawaii. Samples analyzed include those filtered in situ for crustal fluids as well as the CTD samples filtered on deck. Samples were acidified to pH &amp;amp;lt;2 within the autosampler syringe and were purged with nitrogen to remove inorganic carbon. Five to six replicate analyses were performed using an injected sample volume of 150 μL. The detection limits of DOC and TDN were about 2 and 1.5 uM, respectively. Two consensus reference materials CRM, from University of Miami, deep seawater and low carbon water, were used extensively before, between, and after sample analysis to monitor analytical accuracy. The analytical reproducibility for DOC and TDN is better than 1.1 uM and 460 0.2 uM, respectively, by repeated analysis of four deep seawater CRM.&amp;lt;/p&amp;gt;</gco:CharacterString>
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        <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/award/554913.rdf" xlink:title="OCE-1061934" xlink:actuate="onRequest">Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1061934 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1061934</gmx:Anchor>
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        <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/award/554980.rdf" xlink:title="OCE-0939564" xlink:actuate="onRequest">Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-0939564 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=0939564</gmx:Anchor>
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C-DEBI's scientific goals are pursued with a combination of approaches:
(1) coordinate, integrate, support, and extend the research associated with four major programs—Juan de Fuca Ridge flank (JdF), South Pacific Gyre (SPG), North Pond (NP), and Dorado Outcrop (DO)—and other field sites;
(2) make substantial investments of resources to support field, laboratory, analytical, and modeling studies of the deep subseafloor ecosystems;
(3) facilitate and encourage synthesis and thematic understanding of submarine microbiological processes, through funding of scientific and technical activities, coordination and hosting of meetings and workshops, and support of (mostly junior) researchers and graduate students; and
(4) entrain, educate, inspire, and mentor an interdisciplinary community of researchers and educators, with an emphasis on undergraduate and graduate students and early-career scientists.
Note: Katrina Edwards was a former PI of C-DEBI; James Cowen is a former co-PI.
Data Management:
C-DEBI is committed to ensuring all the data generated are publically available and deposited in a data repository for long-term storage as stated in their Data Management Plan (PDF) and in compliance with the NSF Ocean Sciences Sample and Data Policy. The data types and products resulting from C-DEBI-supported research include a wide variety of geophysical, geological, geochemical, and biological information, in addition to education and outreach materials, technical documents, and samples. All data and information generated by C-DEBI-supported research projects are required to be made publically available either following publication of research results or within two (2) years of data generation.
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The U.S. National Science Foundation (NSF) 
Japan's Ministry of Education, Culture, Sports, Science and Technology (MEXT) 
The European Consortium for Ocean Research Drilling (ECORD)

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                            <gco:CharacterString>&lt;p&gt;&lt;em&gt;Description from NSF award abstract:&lt;/em&gt;&lt;br /&gt;
Current estimates suggest that the volume of ocean crust capable of sustaining life is comparable in magnitude to that of the oceans. To date, there is little understanding of the composition or functional capacity of microbial communities in the sub-seafloor, or their influence on the chemistry of the oceans and subsequent consequences for global biogeochemical cycles. This project focuses on understanding the relationship between microbial communities and fluid chemistry in young crustal fluids that are responsible for the transport of energy, nutrients, and organisms in the crust. Specifically, the PIs will couple microbial activity measurements, including autotrophic carbon, nitrogen and sulfur metabolisms as well as mineral oxide reduction, with quantitative assessments of functional gene expression and geochemical transformations in basement fluids. Through a comprehensive suite of in situ and shipboard analyses, this research will yield cross-disciplinary advances in our understanding of the microbial ecology and geochemistry of the sub-seafloor biosphere. The focus of the effort is at North Pond, an isolated sediment pond located on ridge flank oceanic crust 7-8 million years old on the western side of the Mid-Atlantic Ridge. North Pond is currently the target for drilling on IODP expedition 336, during which it will be instrumented with three sub-seafloor basement observatories.&lt;/p&gt;
&lt;p&gt;The project will leverage this opportunity for targeted and distinct sampling at North Pond on two German-US research cruises to accomplish three main objectives:&lt;/p&gt;
&lt;p&gt;1. to determine if different basement fluid horizons across North Pond host distinct microbial communities and chemical milieus and the degree to which they change over a two-year post-drilling period.&lt;/p&gt;
&lt;p&gt;2. to quantify the extent of autotrophic metabolism via microbially-mediated transformations in carbon, nitrogen, and sulfur species in basement fluids at North Pond.&lt;/p&gt;
&lt;p&gt;3. to determine the extent of suspended particulate mineral oxides in basement fluids at North Pond and to characterize their role as oxidants for fluid-hosted microbial communities.&lt;/p&gt;
&lt;p&gt;Specific outcomes include quantitative assessments of microbial activity and gene expression as well as geochemical transformations. The program builds on the integrative research goals for North Pond and will provide important data for guiding the development of that and future deep biosphere research programs. Results will increase understanding of microbial life and chemistry in young oceanic crust as well as provide new insights into controls on the distribution and activity of marine microbial communities throughout the worlds oceans.&lt;/p&gt;
&lt;p&gt;There are no data about microbial communities in ubiquitous cold, oceanic crust, the emphasis of the proposed work. This is an interdisciplinary project at the interface of microbial ecology, chemistry, and deep-sea oceanography with direct links to international and national research and educational organizations.&lt;/p&gt;</gco:CharacterString>
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