<div><p>Adult Acropora palmata colonies were sampled (1 cm<sup>2</sup>) with hammer and chisel and tissues were preserved in 96% non-denature Ethanol. DNA was extracted using the DNEasy tissue kit (Qiagen) following manufacturer’s instructions. Thirteen singleplex Polymerase Chain Reactions (PCR) were performed per sample using fluorescently labeled primers to assay 13 loci containing tri repeats and one di repeat. PCR products were visualized with an automated sequencer (ABI 3730). An internal size standard (Gene Scan 500-Liz, Applied Biosystems CA) ensured accurate sizing. Electropherograms were analyzed with GeneMapper Software 5.0 (Applied Biosystems, CA). Alleles were scored as PCR product size in basepairs.</p>
<p>For quality detailed protocol and quality control please see Pinzon et al 2010 and Baums et al. 2014.</p></div>
Symbiodinium ‘fitti’ multilocus genotypes derived from 13 microsatellite markers
<div><p>Symbiodinium ‘fitti’ (ITS2 clade A3) symbiont genotypes from Acropora palmata</p></div>
Acropora palmata symbiont genotypes
<div><p>Electropherograms of microsatellite alleles were scored for allele sizes (basepairs) in Genemapper vers. 5.0 (Applied Biosystems) and transferred to a Filemaker Database. </p>
<p><strong>BCO-DMO Processing Notes:</strong></p>
<p>- Added PI-provided lat/lon for each "Reef" location.</p>
<p>- Edited primer column where two alleles were present in one column to split into separate columns (i.e., allele size 1 was the first 3 digits, and allele size 2 were the second consecutive digits of a six digit column. These were separated into<br />
separate columns per allele size at each locus).</p>
<p>- Edited entire header to BCO-DMO format (e.g., lowercase names, underscores where needed)</p>
<p>- Edited lat/lon values to four decimal precision consistently.</p>
<p>- Edited spaces in reef names to underscores.</p>
<p>- Edited zero value allele sizes to 'nd' as per PI.</p>
<p>- Split date into year, month, day fromat.</p></div>
636908
Acropora palmata symbiont genotypes
2016-01-28T22:52:58-05:00
2016-01-28T22:52:58-05:00
2023-07-07T16:10:26-04:00
urn:bcodmo:dataset:636908
Symbiodinium genotypes derived from microsatellite markers collected from multiple reefs in the Florida Keys National Marine Sanctuary from 2005-2014 (Surviving Climate Change project)
false
Baums, I. B., Williams, D. E. (2016) Symbiodinium genotypes derived from microsatellite markers collected from multiple reefs in the Florida Keys National Marine Sanctuary from 2005-2014 (Surviving Climate Change project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 2016.01.26) Version Date 2016-01-26 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/636908 [access date]
true
2016.01.26
false
2016-01-26
HTML
https://www.bco-dmo.org/dataset/636908
text/html
Datapackage.json
Frictionless Data Package
https://www.bco-dmo.org/dataset/636908/datapackage.json
application/vnd.datapackage+json
PDF
https://www.bco-dmo.org/dataset/636908/Dataset_description.pdf
application/pdf
JSON-LD
https://www.bco-dmo.org/dataset/636908.json
application/ld+json
Turtle
https://www.bco-dmo.org/dataset/636908.ttl
text/turtle
RDF/XML
https://www.bco-dmo.org/dataset/636908.rdf
application/rdf+xml
ISO 19115-2 (NOAA Profile)
https://www.bco-dmo.org/dataset/636908/iso
application/xml
http://www.isotc211.org/2005/gmd-noaa
Dublin Core
https://www.bco-dmo.org/dataset/636908/dublin-core
application/xml
http://purl.org/dc/elements/1.1/
636908
http://lod.bco-dmo.org/id/dataset/636908
<http://www.opengis.net/def/crs/OGC/1.3/CRS84> POLYGON ((-80.3748 25.0096, -80.2973 25.0096, -80.2973 25.1236, -80.3748 25.1236, -80.3748 25.0096))
JGOFS
http://www.opengis.net/def/crs/OGC/1.3/CRS84
-80.3748
25.0096
-80.2973
25.1236
25.066600000129
-80.336050000407