http://lod.bco-dmo.org/id/dataset/637695
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2016-02-02
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Field conditions during grazing experiments in Kaneohe Bay, HI during 2012-2013 (EAGER: Copepod nauplii project)
2016-02-02
publication
2016-02-02
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2021-01-20
publication
https://doi.org/10.26008/1912/bco-dmo.637695.1
Erica Goetze
University of Hawaiʻi at Mānoa
principalInvestigator
Petra H. Lenz
University of Hawaiʻi at Mānoa
principalInvestigator
Karen E. Selph
University of Hawaiʻi at Mānoa
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Goetze, E., Lenz, P., Selph, K. E. (2021) Field conditions during grazing experiments in Kaneohe Bay, HI during 2012-2013 (EAGER: Copepod nauplii project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2016-02-02 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.637695.1 [access date]
Field conditions during grazing experiments Dataset Description: Methods and Sampling: <p>Five combined bottle incubation and seawater dilution experiments were performed over a two-week period where the in-situ 2-35 µm total cell biomass ranged from 37 – 158 µg C L-1. Both Parvocalanus crassirostris and Bestiolina similis grazed a range of prey types and sizes, and shifted their selectivity of prey groups over the two-week period. In general, P. crassirostris grazed on a wider spectrum of prey than B. similis, which avoided the smallest potential prey (2-5 µm) across all dates. Both species had similar overall grazing rates as well as high daily carbon rations (at times &gt;100%), and selected for the largest cells when they were more abundant. The trophic impact of each species was driven largely by in situ nauplius abundance, which was higher for P. crassirostris, from 0.8 to 8.9 nauplii L-1, than for B. similis, which ranged from 0.2 to 0.8 nauplii L-1.&nbsp; Our results suggest that the two species overlap in their potential prey, however, P. crassirostris appears to target a wider variety of prey, with B. similis preferring larger cells.&nbsp;</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1255697 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1255697
completed
Erica Goetze
University of Hawaiʻi at Mānoa
808-956-7156
Department of Oceanography, University of Hawaii at Manoa 1000 Pope Road
Honolulu
HI
96822
United States
egoetze@hawaii.edu
pointOfContact
Petra H. Lenz
University of Hawaiʻi at Mānoa
808-956-8003
Pacific Biosciences Research Center (PBRC), Bekesy Laboratory of Neurobiology 1993 East-West Road
Honolulu
HI
96822
United States
petra@hawaii.edu
pointOfContact
Karen E. Selph
University of Hawaiʻi at Mānoa
808-956-7941
Department of Oceanography 1000 Pope Road, MSB 205
Honolulu
HI
96822
USA
selph@hawaii.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
date_local
lat
lon
irradiance
wind_spd
wind_dir
sal
temp
chl_a
chl_std_err
theme
None, User defined
date_local
latitude
longitude
irradiance
wind_speed_kts
wind_dir
salinity
water temperature at measurement depth
chlorophyll a
standard error
featureType
BCO-DMO Standard Parameters
Anemometer
Flow Cytometer
Fluorometer
Photosynthetically Available Radiation Sensor
Water Temperature Sensor
instrument
BCO-DMO Standard Instruments
Goetze_2012-2013
service
Deployment Activity
Kaneohe Bay, HI
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
EAGER: New molecular methods for studying copepod nauplii in the field
https://osprey.bco-dmo.org/project/473049
EAGER: New molecular methods for studying copepod nauplii in the field
<p><em>Description from NSF Award Abstract:</em><br />
The most abundant metazoans in the open sea are often the earliest developmental stages of copepods, their nauplii. Nauplii remain under-studied due to the limitations of conventional techniques and an historical emphasis on studying the larger mesozooplankton. However, there is increasing recognition that nauplii play important roles in food web dynamics, and considerable evidence that nauplii may be important trophic intermediaries between microbial and classical food webs due to their high abundance, high weight-specific ingestion rates, and ability to feed on relatively small particles. This team of investigators is developing a novel molecular approach to studying diverse populations of nauplii in mixed field samples based on quantitative Polymerase Chain Reaction (qPCR). They propose to complete development and validation of this qPCR-based technique for enumeration of nauplii, and evaluate its utility in the field. The specific objectives of this research are to identify and reduce technical and biological sources of error in the methodology, determine the accuracy of the method across a range of environmental conditions, and complete one paired field experiment that compares the grazing impact of naupliar and protozoan micro-grazers in a model subtropical coastal ecosystem.</p>
<p><strong>Note:</strong> This project is funded by an NSF EAGER award.</p>
<p><em>Related publications:</em><br />
Jungbluth, M.J., Goetze, E., and Lenz, P.H. 2013. Measuring copepod naupliar abundance in a subtropical bay using quantitative PCR. Marine Biology, 160: 3125-3141. doi: <a href="http://dx.doi.org/10.1007/s00227-013-2300-y" target="_blank">10.1007/s00227-013-2300-y</a></p>
<p>Jungbluth, M.J., and Lenz, P.H. 2013. Copepod diversity in a subtropical bay based on a fragment of the mitochondrial COI gene. Journal of Plankton Research, 35(3): 630-643. doi: <a href="http://dx.doi.org/10.1093/plankt/fbt015" target="_blank">10.1093/plankt/fbt015</a></p>
EAGER: Copepod nauplii
largerWorkCitation
project
eng; USA
biota
oceans
Kaneohe Bay, HI
-157.7797
-157.7797
21.4322
21.4322
2013-05-27
2013-06-05
Kaneohe Bay, Oahu, Hawaii
0
BCO-DMO catalogue of parameters from Field conditions during grazing experiments in Kaneohe Bay, HI during 2012-2013 (EAGER: Copepod nauplii project)
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/637710.rdf
Name: date_local
Units: yyyy-mm-dd
Description: <p>local date</p>
http://lod.bco-dmo.org/id/dataset-parameter/637711.rdf
Name: lat
Units: decimal degrees
Description: <p>latitude; north is positive</p>
http://lod.bco-dmo.org/id/dataset-parameter/637712.rdf
Name: lon
Units: decimal degrees
Description: <p>longitude; east is positive</p>
http://lod.bco-dmo.org/id/dataset-parameter/637713.rdf
Name: irradiance
Units: uE/sec/m^2
Description: <p>solar irradiance as measured at HIMB</p>
http://lod.bco-dmo.org/id/dataset-parameter/637714.rdf
Name: wind_spd
Units: knots
Description: <p>wind speed</p>
http://lod.bco-dmo.org/id/dataset-parameter/637715.rdf
Name: wind_dir
Units: unitless
Description: <p>wind direction: N=north; S=south; E=east; W=west</p>
http://lod.bco-dmo.org/id/dataset-parameter/637716.rdf
Name: sal
Units: PSU
Description: <p>salinity at 2 meters depth</p>
http://lod.bco-dmo.org/id/dataset-parameter/637717.rdf
Name: temp
Units: degees Celsius
Description: <p>water temperature</p>
http://lod.bco-dmo.org/id/dataset-parameter/637718.rdf
Name: chl_a
Units: micrograms/liter
Description: <p>chlorophyll</p>
http://lod.bco-dmo.org/id/dataset-parameter/637719.rdf
Name: chl_std_err
Units: micrograms/liter
Description: <p>chlorophyll standard error</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
385
https://darchive.mblwhoilibrary.org/bitstream/1912/26595/1/dataset-637695_kaneohe-bay-physical-conditions__v1.tsv
download
https://doi.org/10.26008/1912/bco-dmo.637695.1
download
onLine
dataset
<p>Five combined bottle incubation and seawater dilution experiments were performed over a two-week period where the in-situ 2-35 µm total cell biomass ranged from 37 – 158 µg C L-1. Both Parvocalanus crassirostris and Bestiolina similis grazed a range of prey types and sizes, and shifted their selectivity of prey groups over the two-week period. In general, P. crassirostris grazed on a wider spectrum of prey than B. similis, which avoided the smallest potential prey (2-5 µm) across all dates. Both species had similar overall grazing rates as well as high daily carbon rations (at times &gt;100%), and selected for the largest cells when they were more abundant. The trophic impact of each species was driven largely by in situ nauplius abundance, which was higher for P. crassirostris, from 0.8 to 8.9 nauplii L-1, than for B. similis, which ranged from 0.2 to 0.8 nauplii L-1.&nbsp; Our results suggest that the two species overlap in their potential prey, however, P. crassirostris appears to target a wider variety of prey, with B. similis preferring larger cells.&nbsp;</p>
Specified by the Principal Investigator(s)
<p><strong>BCO-DMO Processing:</strong></p>
<p>- added conventional header with dataset name, PI name, version date, reference information<br />
- renamed parameters to BCO-DMO standard<br />
- reformatted date from d-Mon-yy to yyyy-mm-dd<br />
- transformed table rows and columns<br />
- added lat/lon columns</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
PI Supplied Instrument Name: Instrument Name: Anemometer Instrument Short Name:Anemometer Instrument Description: An anemometer is a device for measuring the velocity or the pressure of the wind. It is commonly used to measure wind speed. Aboard research vessels, it is often mounted with other meteorological instruments and sensors. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/101/
PI Supplied Instrument Name: Instrument Name: Flow Cytometer Instrument Short Name:Flow Cytometer Instrument Description: Flow cytometers (FC or FCM) are automated instruments that quantitate properties of single cells, one cell at a time. They can measure cell size, cell granularity, the amounts of cell components such as total DNA, newly synthesized DNA, gene expression as the amount messenger RNA for a particular gene, amounts of specific surface receptors, amounts of intracellular proteins, or transient signalling events in living cells.
(from: http://www.bio.umass.edu/micro/immunology/facs542/facswhat.htm) Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB37/
PI Supplied Instrument Name: Instrument Name: Fluorometer Instrument Short Name:Fluorometer Instrument Description: A fluorometer or fluorimeter is a device used to measure parameters of fluorescence: its intensity and wavelength distribution of emission spectrum after excitation by a certain spectrum of light. The instrument is designed to measure the amount of stimulated electromagnetic radiation produced by pulses of electromagnetic radiation emitted into a water sample or in situ. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/113/
PI Supplied Instrument Name: Instrument Name: Photosynthetically Available Radiation Sensor Instrument Short Name:PAR sensor Instrument Description: A PAR sensor measures photosynthetically available (or active) radiation. The sensor measures photon flux density (photons per second per square meter) within the visible wavelength range (typically 400 to 700 nanometers). PAR gives an indication of the total energy available to plants for photosynthesis. This instrument name is used when specific type, make and model are not known. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/122/
PI Supplied Instrument Name: Instrument Name: Water Temperature Sensor Instrument Short Name:Water Temp Sensor Instrument Description: General term for an instrument that measures the temperature of the water with which it is in contact (thermometer). Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/134/
Deployment: Goetze_2012-2013
Goetze_2012-2013
lab UHawaii_SOEST
laboratory
Goetze_2012-2013
Erica Goetze
University of Hawaiʻi at Mānoa
lab UHawaii_SOEST
laboratory