{"@context":{"content":"http:\/\/purl.org\/rss\/1.0\/modules\/content\/","dc":"http:\/\/purl.org\/dc\/terms\/","foaf":"http:\/\/xmlns.com\/foaf\/0.1\/","og":"http:\/\/ogp.me\/ns#","rdfs":"http:\/\/www.w3.org\/2000\/01\/rdf-schema#","sioc":"http:\/\/rdfs.org\/sioc\/ns#","sioct":"http:\/\/rdfs.org\/sioc\/types#","skos":"http:\/\/www.w3.org\/2004\/02\/skos\/core#","xsd":"http:\/\/www.w3.org\/2001\/XMLSchema#","owl":"http:\/\/www.w3.org\/2002\/07\/owl#","rdf":"http:\/\/www.w3.org\/1999\/02\/22-rdf-syntax-ns#","rss":"http:\/\/purl.org\/rss\/1.0\/","site":"https:\/\/www.bco-dmo.org\/ns#","odo":"http:\/\/ocean-data.org\/schema\/","emo":"http:\/\/ocean-data.org\/schema\/entity-matching#","bibo":"http:\/\/purl.org\/ontology\/bibo\/","crypto":"http:\/\/id.loc.gov\/vocabulary\/preservation\/cryptographicHashFunctions\/","bcodmo":"http:\/\/lod.bco-dmo.org\/id\/","tw":"http:\/\/tw.rpi.edu\/schema\/","dcat":"http:\/\/www.w3.org\/ns\/dcat#","time":"http:\/\/www.w3.org\/2006\/time#","geo":"http:\/\/www.w3.org\/2003\/01\/geo\/wgs84_pos#","geosparql":"http:\/\/www.opengis.net\/ont\/geosparql#","sf":"http:\/\/www.opengis.net\/ont\/sf#","void":"http:\/\/rdfs.org\/ns\/void#","sd":"http:\/\/www.w3.org\/ns\/sparql-service-description#","dctype":"http:\/\/purl.org\/dc\/dcmitype\/","prov":"http:\/\/www.w3.org\/ns\/prov#","schema":"http:\/\/schema.org\/","geolink":"http:\/\/schema.geolink.org\/1.0\/base\/main#","spdx":"http:\/\/spdx.org\/rdf\/terms#","bcodmo_vocab":"http:\/\/schema.bco-dmo.org\/"},"@id":"http:\/\/lod.bco-dmo.org\/id\/dataset\/639794#graph","@graph":[{"http:\/\/lod.bco-dmo.org\/id\/dataset\/639794":{"@id":"http:\/\/lod.bco-dmo.org\/id\/dataset\/639794","@type":["http:\/\/ocean-data.org\/schema\/DeploymentDatasetCollection","http:\/\/www.w3.org\/ns\/dcat#Dataset","http:\/\/ocean-data.org\/schema\/Dataset"],"http:\/\/ocean-data.org\/schema\/hasAcquisitionDescription":[{"@value":"
Methods for May Cohorts (from Waldbusser et al.)<\/strong> Setting and Experimental Design<\/strong> During each tank change, all larvae were captured on an appropriately\u00a0sized sieve and a primary sample of the entire cohort was randomly collected with a clean stainless steel spatula. The mass of each primary sample varied from ~0.5 to 1.0 g representing an estimated 2.8 x 106<\/sup> to 5.6 x 106<\/sup> two-day old larvae or 2.3 x 105<\/sup> to 4.6 x 105<\/sup> 19-day old larvae. All 400 million larvae in the cohort were reared collectively in one tank, so replicate primary samples would have been pseudo-replicates of larvae from the same tank.<\/p>\n The larval samples were quickly rinsed with deionized water, immediately frozen, and freeze dried within 2 weeks with a Labconco FreeZone 6 Freeze Drier. Sub-samples from each primary sample were then used for subsequent analyses. Approximately 100\u2019s to 10\u2019s of larvae for stable isotope analyses of shell carbonates from\u00a0 Day 2 and 19 samples were needed, and lipid extractions required roughly 104<\/sup> to 105<\/sup> larvae for Day 2 and 19, respectively. Analyses of stable isotopes and elemental analyses of tissue required approximately 103<\/sup> to 104<\/sup> larvae. Variable volumes (200-800 ml) of algal cultures were vacuum filtered onto pre-combusted onto glass fiber filters (GF\/F 47mm, Whatman) to achieve a similar analytical mass for all analyses.<\/p>\n \u03b413<\/sup>C Measurements<\/em><\/strong> Water samples were analyzed by continuous mass flow spectrometry on a GasBench-Delta V system. The run had a precision of 0.02\u2030 and accuracy better than 0.06\u2030. Larval shell carbonate \u03b413<\/sup>C was measured by reacting the freeze-dried larvae with a viscous ~105% H3<\/sub>PO4 <\/sub>solution in a Finnigan MAT Kiel III at 70 degrees\u00a0C for 5 minutes after which the isotopic composition of the evolved and purified CO2<\/sub> was measured on a dual inlet mass spectrometer (MAT 252 IRMS).\u00a0 Each run had a precision and accuracy of 0.02\u2030 and 0.01 \u2030, respectively. Repeatability of larval shell samples was within 0.02\u2030. To obtain measures of tissue \u03b413<\/sup>C composition of tissues, freeze-dried larvae acid fumed with concentrated HCl to eliminate the shell carbonate signal. The remaining sample material was then analyzed by flash combustion (>1000 degree\u00a0C) using a Carlo Erba NA 1500 elemental analyzer interfaced by continuous-flow mass spectrometer (Delta-Plus XL). Repeatability of organic larval samples was 0.21\u2030. Algae samples, collected as noted above, were analyzed in the same way as larval tissue however without the acid fuming. Measurements of organic \u03b413<\/sup>C composition were made with a precision and accuracy of 0.17\u2030 and 0.12\u2030, respectively.<\/p>\n Larval Biochemical and Size Analyses<\/em><\/strong> Methods for August Cohorts (from Brunner et al.)<\/strong> Setting and Experimental Design<\/em><\/strong> Spawn and Larval Culture<\/em><\/strong> All larvae were fed a rotating mixture of algal cultures grown at WCH including Isochrysis sp<\/em>., Chaetoceros gracilis<\/em>, and Chaetoceros calcitrans<\/em>. In the first 5-10 days the larvae were fed Isochrysis sp<\/em> from a continuous culture bag system and these algae have a particularly low \u03b413<\/sup>C signal [around -45\u2030] which is due to the bubbling of culture water with compressed industrial grade CO2<\/sub>. At each feeding, between 200 and 800 mL of algal feed cultures were filtered onto pre-combusted 47mm GF\/F glass fiber filters (Whatman) and the algae retained on the filter dried at <45 degrees\u00a0C for storage until analysis. The sample volume was determined to account for different densities of algal culture and achieve optimal amounts of organic material on each filter for analysis.<\/p>\n At each tank water change, the previous tank\u2019s water was drained onto an appropriately sized sieve to capture all the larvae, and the larvae were then transferred into a newly-filled tank. A 0.5 \u2013 2 g dry weight sub-sample (representing an estimated 3-10 x\u00a0106<\/sup> two-day old larvae or 2-9 x\u00a0105<\/sup> 19-day old larvae) of the cohort was collected from the sieve, briefly rinsed with DI water and immediately frozen. The samples were then freeze-dried within three weeks of collection (Labconco FreeZone 6 Freeze Drier). As all the larvae from each cohort were living in the same tank, taking multiple primary samples would have been pseudo-replication, and the investigators\u00a0therefore assume that the sub-samples accurately represent the entire larval cohort. In May, after 5 days, the fastest growing larvae were selected by passing the cohort through an 80 um sieve, which caught about half of the larvae. This procedure for poorly performing cohorts commonly employed at WCH. The August cohorts were not sorted via this size-fraction screening as their general growth and fitness was better.<\/p>\n Isotopic and Compositional Measurements of Larvae and Algae<\/em><\/strong> To measure larval tissue \u03b413<\/sup>C and \u03b415<\/sup>N, 6-10 mg freeze-dried larvae in Ag boats were acidified in a concentrated HCl atmosphere following the method of Hedges & Stern (1984). The samples were dried overnight, encapsulated in Sn boats, and loaded into a Costech Zero Blank Autosampler. Samples were flash combusted at >1020 degrees\u00a0C using a Carlo Erba NA1500 Elemental Analyzer (Verardo et al. 1990) and the resulting gas was analyzed by continuous-flow mass spectrometry using a DeltaPlus XL isotope ratio mass spectrometer. Hole punches of filter (diameter ~7mm) with dried algae were analyzed via the same method as the larval tissue, without the acidification step. Repeatability of samples was 0.21\u2030 for \u03b413<\/sup>C, and 0.27\u2030 for \u03b415<\/sup>N.<\/p>\n In order to measure larval shell carbonate \u03b413<\/sup>C, the investigators\u00a0reacted 19-50 freeze-dried larvae with concentrated phosphoric acid at 70 degrees\u00a0C for 5 minutes in a Finnigan Keil III device. The resulting CO2<\/sub> was isolated from other reaction products and measured on a MAT 252 mass spectrometer by dual inlet mass spectrometry. Accuracy was better than \u00b10.05\u2030, and repeatability was \u00b10.02 \u2030.<\/p>\n Larval samples were also analyzed for organic and inorganic elemental composition before and after acidification as described above on a Carlo Erba NA-1500 elemental analyzer calibrated with at least 6 acetanilide standards following the methods of Verardo et al. (1990). Ash-free dry weight (AFDW) was determined by weight comparison before and after combustion at 490 degrees\u00a0C for 4 hours.<\/p>\n Extractable Lipids, Length and SEM Imaging<\/em><\/strong> To measure extractable lipids, ~75 mg samples of freeze-dried larvae were extracted on a Dionex ASE 200 Accelerated Solvent Extractor for four cycles of 5 minutes at 1500 psi with 3:1 <\/em>methylene chloride:methanol.\u00a0The extracts were combined with 25 mL hexane and washed against 10 mL 50% saturated NaCl to remove hydrophilic impurities (proteins, carbohydrates). The aqueous phase was drained away and the lipid-containing fraction was dried under an N2<\/sub> gas stream. The lipid concentrate was then re-suspended in 500 uL CS2 <\/sub>and an aliquot (100 uL) moved to a tared nickle weighing boat, dried and re-weighed to get a gravimetric weight of lipid. This weight was compared to the original sample weight to find the fraction total lipid. As with the other bulk measurements, these values were normalized to the larval weights to estimate lipid (ng) per larvae. <\/em>Additionally, it should be noted that the lipid measurements by Waldbusser et al. (2013) may be an over-estimate, due to the gravimetric extraction method used. The washed values presented here provide a better estimate of the true lipid content. Due to a sampling error, the investigators\u00a0do not have a reliable washed lipid value for the 48 hour time point for the May cohort. Given the similarity between the washed and unwashed values in early life, the unwashed gravimetric lipid weight (as reported in Waldbusser et al. 2013) are used instead.<\/p>\n Carbonate chemistry<\/em><\/strong> Samples for PCO2<\/sub> and dissolved inorganic carbon (DIC) analysis were collected at each water change in 330 mL amber glass bottles, poisoned with HgCl2<\/sub> and analyzed following Bandstra et al. (2006) The uncertainty of these measurements is less than 2% and 0.2% for PCO2<\/sub> and DIC, respectively. The remaining carbonate system parameters were calculated using the carbonic acid dissociation constants of Millero (2010), the boric acid constants as defined by Dickson (1990), and the aragonite solubility as defined by Mucci (1983).<\/p><\/div>","@type":"rdf:HTML"}],"http:\/\/ocean-data.org\/schema\/hasBriefDescription":[{"@value":"Biochemistry of 3 pacific oyster cohorts from Whiskey Creek Hatchery.","@language":"en-US"}],"http:\/\/purl.org\/dc\/terms\/description":[{"@value":" Biochemistry summary\u00a0of 3 pacific oyster cohorts from Whiskey Creek Hatchery.\u00a0All data are presented in Table 4 of Brunner et al. (in review).<\/p>\n All details on sample collection, methods, and analytical techniques may be found in: The original file wass split into 3 spreadsheets containing three different, but related datasets Across all three datasets, there are 3 cohorts of pacific oyster larvae that have been sampled, one in May and two in August, one in untreated seawater, the other in buffered seawater.\u00a0Time is all indexed to fertilization of the oysters, with samples collected on days noted.\u00a0<\/p><\/div>","@type":"rdf:HTML"}],"http:\/\/www.w3.org\/2000\/01\/rdf-schema#label":[{"@value":"Pacific oyster cohort tracking data - Biochemistry","@type":"xsd:string"}],"http:\/\/ocean-data.org\/schema\/hasProcessingDescription":[{"@value":" The derived variables in the Biochemistry dataset\u00a0are Est. Weight (weight_est), Est. Lipid (lipid_est), Tissue atomic C:N (tissue_C_to_N), and calcification rate (calcif_rate). These are computed as follows:<\/p>\n Estimated Weight -\u00a0computed from Bochenek et al. (2001) weight at size model, initially assuming fixed proportion of organic and inorganic components to compute total weight.<\/p>\n Total weight = (6.745*(10-4)*(shell length2.557))*4<\/p>\n Est Lipid - fraction lipid multiplied by estimated total weight.<\/p>\n Tissue atomic C:N is the molar ratio of tissue carbon and nitrogen.<\/p>\n Calcification rate is computed by the change in shell mass divided by number days between measurements.<\/p>\n BCO-DMO Processing:
\nThe investigators measured the isotopic composition of carbon in larval shell, larval tissue, food, and holding tank water during growth and development of a commercial larval Crassostrea gigas <\/em>cohort in May 2011. These measurements were\u00a0coupled to bulk measures of larval biochemical composition to estimate energetic status and demands for the initial shell development.\u00a0<\/p>\n
\nCrassostrea gigas<\/em> larvae were raised according to commercial spawning protocols at Whiskey Creek Shellfish Hatchery in Netarts Bay, Oregon, as previously described in detail in Barton et al., 2012. Briefly, successfully fertilized Willapa Bay oyster larvae from several conditioned broodstock oysters were added to a 22 cubic meter\u00a0tank an hour after fertilization at an estimated density of approximately 5 x 107<\/sup> individuals per cubic meter. Water was changed in culture tanks every 2-3 days during cohort development. The pH of fertilization water was 8.15 (measured with a YSI 30 on the NBS scale), and developing embryos were reared under a PCO2<\/sub> of 396 uatm and aragonite saturation state of 2.38. Samples for PCO2<\/sub> and TCO2<\/sub> of culture tanks were collected at each tank filling and analyzed following Bandstra et al. (2006)\u00a0with an uncertainty of less than 5% and 0.2% for PCO2<\/sub> and TCO2, respectively. The rest of the carbonate system was calculated using standard dissociation constants as previously described Barton et al., 2012. Conditions in culture tanks at filling had an average PCO2<\/sub> (\u00b1 1 S.D.) of 364 (\u00b1 40) uatm aragonite saturation state with of 2.58 (\u00b1 0.33) at the culture temperature of 25 degrees\u00a0C, and a salinity of 26.5 (\u00b1 2.29). Larvae were fed a diet of Isocrysis galbani<\/em> cultured in a continuous culture system during the first week following fertilization and this was switched to a mixed algae diet consisting primarily of Chaetocerous gracilis, Thalassiorsira spp., <\/em>and Isochrysis galbana<\/em> cultured in batches. Estimated cell densities were 5-7 x 1010<\/sup> cells per cubic meter.<\/p>\n
\nAll stable carbon isotope samples were analyzed in Oregon State University\u2019s CEOAS Stable Isotope Laboratory and details for standard analyses may be found at\u00a0http:\/\/stable-isotope.coas.oregonstate.edu\/<\/a>. All runs were calibrated and checked with NIST certified (NBS 19, NBS 20 UQ6) and in-house calibrated standards, with precision and accuracy noted below.\u00a0<\/p>\n
\nFractions of organic tissue and shell (% of dry weight) were determined by first measuring %C on bulk freeze-dried larval samples and then decalcified samples (via HCl fuming) on a Carlo Erba NA1500 elemental analyzer. The difference between whole and decalcified larvae and the molar proportion of C in calcium carbonate were used to estimate the % calcium carbonate by weight, with the remainder being organic matter. These values for % organic matter were correlated to (r2<\/sup> = 0.89) loss on ignition (LOI) measurements, with LOI consistently over estimating % organic matter. Total lipid % was measured by extracting freeze-dried larvae with a methylene chloride: methanol solution (3:1) using a Dionex ASE 200 Accelerated Solvent Extractor and weighing an aliquot of the isolated total extractable lipid (TEL) fraction dissolved in CS2<\/sub> gravimetrically. Larval size was evaluated on 100-150 larvae per sample, taken as a separate sub-sample during tank water changes and evaluated on digital micrographs using standard image analysis techniques (ImageJ 1.44p).\u00a0 A standard mass at length model for C. gigas<\/em> larvae (Bocheneck et al<\/em>., 2001)\u00a0was used to calculate a total individual mass. Measured proportional composition data were then applied to the mass at length estimate to compute per larva weights of shell, bulk organic, and lipid content.<\/p>\n
\nThe investigators\u00a0sampled multiple commercial cohorts of Pacific oyster larvae, Crassostrea gigas<\/em>, at the Whiskey Creek Shellfish Hatchery (WCH), Netarts, Oregon, U.S.A. where carbonate chemistry within the hatchery is set by the ambient Netarts Bay conditions, but food availability (ad libitum<\/em>) and temperature (25 degrees\u00a0C) are controlled to optimal levels. The large (~400 million larvae) cohorts provided sufficient larvae to measure bulk biochemistry parameters such as organic content and composition while the sequential tank incubations allowed us to fully constrain the possible isotopic sources for incorporation into shell and tissue at discrete intervals during larval life (e.g., 0-2 days, 2-5 days).<\/p>\n
\nThe investigators followed three cohorts of approximately 400 million Crassostrea gigas<\/em> larvae from fertilization through metamorphosis: one cohort in May 2011, and two in August 2011. WCH\u2019s spawning protocols are described in detail by Barton et al. (2012), and by Waldbusser et al. (2013). Briefly, larvae are maintained in static 22 cubic meter\u00a0tanks, which have their water changed every 2-3 days, and are fed daily. The water for each tank change is pumped from Netarts Bay \u2014 a marine-dominated estuary on the northern Oregon coast (45.403N, 123.944W) which is flushed nearly every tidal cycle with water from the adjacent North Pacific Ocean (Whiting & McIntire 1985). With roughly two-thirds of the 9.41 square-kilometer\u00a0<\/sup>aerial extent covered in Zostrera spp. <\/em>beds, and the remaining tidal flats supporting microphytobenthos production, there is a diurnal PCO2<\/sub> cycle that reflects the daily cycle of photosynthesis and respiration in Spring and Summer such that the highest PCO2<\/sub> values are seen in the morning after a night of respiration and the lowest PCO2<\/sub> values are seen in the afternoon (Waldbusser & Salisbury 2014). Thus, the saturation state at the hatchery intake pipe is variable on hourly, weekly and seasonal timescales (Barton et al. 2012, Waldbusser & Salisbury 2014). To exaggerate the naturally-occurring PCO2<\/sub> conditions of the upwelling season in August, water was pumped in the morning. May 2011 was a period of high freshwater input into Netarts Bay and thus the water started with a lower salinity (~26) and thus alkalinity than usual. For the May cohort, water for each tank-fill was pumped in the afternoon, when PCO2<\/sub> was relatively low. All water is heated to 25 degree\u00a0C before being pumped into the tanks.<\/p>\n
\nOn 18 May 2011 and again on 14 August 2011 at least 3 Willapa Bay females were strip-spawned and fertilized with sperm from at least one Willapa Bay male. The fertilized eggs in August were split into two different tanks ~ 1 hr after fertilization (a \"buffered\"\u00a0and control, each starting with approximately 400 million larvae), and followed separately for the remainder of their larval period. The WCH buffer system treated incoming water with industrial grade soda ash (Na2<\/sub>CO3<\/sub>) to an aragonite saturation state (\u03a9ar<\/sub>) equal to 4 during this experiment.<\/p>\n
\nAll stable carbon isotope samples were analyzed in Oregon State University\u2019s College of Earth, Ocean, and Atmospheric Sciences Stable Isotope Laboratory. Detailed descriptions of these standard protocols can be found in Waldbusser et al. (2013) and at http:\/\/stable-isotope.coas.oregonstate.edu\/<\/a>. All runs were calibrated and checked with NIST certified and in-house calibrated standards. Precision and accuracy are noted for each analysis.<\/p>\n
\nAt each tank change, an aliquot of larvae were fixed in 2.5% gluteraldehyde and 1% paraformaldehyde in a 0.1 M cacodylate buffer. Samples were later transferred to 50% ethanol and, imaged using an Olympus SZH10 dissecting microscope. 100 larval lengths per sampling point were measured (ImageJ 1.44p). Lengths were converted into weights using the dry-tissue weight to length relationship for larval C. gigas<\/em> from Bochenek et al. (2001), accounting for our measured proportion shell (1-AFDW) at each time point, instead of the 75% shell assumption used by the model.<\/p>\n
\nater samples were collected at each tank filling prior to the addition of larvae or algae. For dissolved inorganic carbon (DIC) 13<\/sup>C analysis, aliquots of HgCl2 <\/sub>-fixed sample were pipetted into Labco glass vials sealed with rubber septa caps. The vials were cooled to 13 degrees\u00a0C, allowed to equilibrate for 15 minutes, and purged with He for 5 minutes each. 85% phosphoric acid was then added to each vial via syringe. The samples were left to equilibrate for 10 hours and analyzed via continuous-flow mass spectrometry using a GasBench-DeltaV system. Each run was standardized using a combination of sodium bicarbonate (3 mM in solution) and calcium carbonate standards. These methods have a precision of 0.15 \u2030 and repeatability better than 0.2 \u2030.<\/p>\n
\nWaldbusser, G.G., E. L. Brunner,B.A. Haley, B. Hales, C. J. Langdon, and F. G. Prahl.\u00a02013.\u00a0A developmental and energetic basis linking larval oyster shell formation to ocean acidification. Geophysical Research Letters 40: 2171-2176.\u00a0doi:10.1002\/grl.50449<\/a>
\nBrunner, E.L., F.G. Prahl, B. Hales, G.G. Waldbusser (in review).\u00a0Insights from Stable Isotopes into the Sensitivity of Larval Pacific Oysters to Ocean Acidification. Marine Ecology Progress Series.<\/p>\n
Isotope Data Summary<\/a>
\nBiochem Summary
Carbonate Chemistry Summary<\/a><\/p>\n
\n- modified parameter names to conform with BCO-DMO naming conventions;
\n- replaced blanks (missing data) with \"nd\", meaning \"no data\";
\n- replaced \"Aug\" with \"August\" in cohort name column;
\n- 04-April-2018: removed embargo on dataset.<\/p><\/div>","@type":"rdf:HTML"}],"http:\/\/purl.org\/dc\/terms\/identifier":[{"@value":"639794","@type":"xsd:int"}],"http:\/\/purl.org\/dc\/terms\/title":[{"@value":"Pacific oyster cohort tracking data - Biochemistry"}],"http:\/\/purl.org\/dc\/terms\/date":[{"@value":"2016-03-08T12:44:21-05:00","@type":"xsd:dateTime"}],"http:\/\/purl.org\/dc\/terms\/created":[{"@value":"2016-03-08T12:44:21-05:00","@type":"xsd:dateTime"}],"http:\/\/purl.org\/dc\/terms\/modified":[{"@value":"2023-07-07T16:10:26-04:00","@type":"xsd:dateTime"}],"http:\/\/rdfs.org\/ns\/void#inDataset":[{"@id":"http:\/\/www.bco-dmo.org\/"}],"http:\/\/ocean-data.org\/schema\/namedGraph":[{"@value":"urn:bcodmo:dataset:639794","@type":"xsd:token"}],"http:\/\/ocean-data.org\/schema\/osprey_page":[{"@id":"https:\/\/www.bco-dmo.org\/dataset\/639794"}],"http:\/\/ocean-data.org\/schema\/identifier":[{"@value":"_:Identifier639794"}],"http:\/\/ocean-data.org\/schema\/datasetTitle":[{"@value":"Biochemistry of 3 pacific oyster cohorts from Whiskey Creek Hatchery in Netarts Bay, OR, USA from 2009-2011","@language":"en-US"}],"http:\/\/ocean-data.org\/schema\/abstract":[{"@value":"","@language":"en-US"}],"http:\/\/purl.org\/dc\/terms\/rights":[{"@id":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"http:\/\/ocean-data.org\/schema\/deprecated":[{"@value":"false","@type":"xsd:boolean"}],"http:\/\/purl.org\/dc\/terms\/bibliographicCitation":[{"@value":"Waldbusser, G. G. (2016) Biochemistry of 3 pacific oyster cohorts from Whiskey Creek Hatchery in Netarts Bay, OR, USA from 2009-2011. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 11 Feb 2016) Version Date 2016-02-11 [if applicable, indicate subset used]. http:\/\/lod.bco-dmo.org\/id\/dataset\/639794 [access date]","@type":"xsd:string"}],"http:\/\/ocean-data.org\/schema\/validated":[{"@value":"true","@type":"xsd:boolean"}],"http:\/\/ocean-data.org\/schema\/versionLabel":[{"@value":"11 Feb 2016","@type":"xsd:string"}],"http:\/\/ocean-data.org\/schema\/currentState":[{"@id":"http:\/\/lod.bco-dmo.org\/id\/dataset-current-state\/7"}],"http:\/\/ocean-data.org\/schema\/nodcTopic":[{"@id":"http:\/\/lod.bco-dmo.org\/id\/nodc-dataset-topic\/150"},{"@id":"http:\/\/lod.bco-dmo.org\/id\/nodc-dataset-topic\/156"}],"http:\/\/ocean-data.org\/schema\/datasetType":[{"@id":"http:\/\/lod.bco-dmo.org\/id\/dataset-type\/157"}],"http:\/\/ocean-data.org\/schema\/restricted":[{"@value":"false","@type":"xsd:boolean"}],"http:\/\/ocean-data.org\/schema\/hasAward":[{"@id":"http:\/\/lod.bco-dmo.org\/id\/award\/54838"}],"http:\/\/ocean-data.org\/schema\/fromInstrument":[{"@id":"http:\/\/lod.bco-dmo.org\/id\/dataset-instrument\/639802"},{"@id":"http:\/\/lod.bco-dmo.org\/id\/dataset-instrument\/639800"},{"@id":"http:\/\/lod.bco-dmo.org\/id\/dataset-instrument\/639801"},{"@id":"http:\/\/lod.bco-dmo.org\/id\/dataset-instrument\/639803"}],"http:\/\/ocean-data.org\/schema\/storesValuesFor":[{"@id":"http:\/\/lod.bco-dmo.org\/id\/dataset-parameter\/639805"},{"@id":"http:\/\/lod.bco-dmo.org\/id\/dataset-parameter\/639806"},{"@id":"http:\/\/lod.bco-dmo.org\/id\/dataset-parameter\/639807"},{"@id":"http:\/\/lod.bco-dmo.org\/id\/dataset-parameter\/639808"},{"@id":"http:\/\/lod.bco-dmo.org\/id\/dataset-parameter\/639809"},{"@id":"http:\/\/lod.bco-dmo.org\/id\/dataset-parameter\/639810"},{"@id":"http:\/\/lod.bco-dmo.org\/id\/dataset-parameter\/639811"},{"@id":"http:\/\/lod.bco-dmo.org\/id\/dataset-parameter\/639812"},{"@id":"http:\/\/lod.bco-dmo.org\/id\/dataset-parameter\/639813"},{"@id":"http:\/\/lod.bco-dmo.org\/id\/dataset-parameter\/639814"},{"@id":"http:\/\/lod.bco-dmo.org\/id\/dataset-parameter\/639815"},{"@id":"http:\/\/lod.bco-dmo.org\/id\/dataset-parameter\/639816"},{"@id":"http:\/\/lod.bco-dmo.org\/id\/dataset-parameter\/639817"},{"@id":"http:\/\/lod.bco-dmo.org\/id\/dataset-parameter\/639818"}],"http:\/\/ocean-data.org\/schema\/hasAgentWithRole":[{"@id":"http:\/\/lod.bco-dmo.org\/id\/person-role\/639797"},{"@id":"http:\/\/lod.bco-dmo.org\/id\/person-role\/639798"}],"http:\/\/purl.org\/dc\/terms\/language":[{"@value":"http:\/\/id.loc.gov\/vocabulary\/iso639-1\/en","@type":"xsd:anyURI"}],"http:\/\/xmlns.com\/foaf\/0.1\/homepage":[{"@id":"https:\/\/www.bco-dmo.org\/dataset\/639794"}],"http:\/\/purl.org\/dc\/terms\/issued":[{"@value":"2016-02-11","@type":"xsd:date"}],"http:\/\/purl.org\/dc\/terms\/publisher":[{"@id":"http:\/\/lod.bco-dmo.org\/id\/affiliation\/191"}],"http:\/\/www.w3.org\/ns\/dcat#contactPoint":[{"@id":"http:\/\/lod.bco-dmo.org\/id\/person\/51380"}]},"_:html639794":{"@id":"_:html639794","@type":["http:\/\/ocean-data.org\/schema\/MetadataViewAffordance"],"http:\/\/schema.org\/subjectOf":[{"@id":"http:\/\/lod.bco-dmo.org\/id\/dataset\/639794"}],"http:\/\/schema.org\/name":[{"@value":"HTML","@type":"xsd:string"}],"http:\/\/ocean-data.org\/schema\/affordedBy":[{"@id":"http:\/\/lod.bco-dmo.org\/id\/affiliation\/191"}],"http:\/\/schema.org\/target":[{"@value":"_:html639794entryPoint"}]},"_:html639794entryPoint":{"@id":"_:html639794entryPoint","@type":["http:\/\/schema.org\/EntryPoint","http:\/\/ocean-data.org\/schema\/HtmlLandingPage"],"http:\/\/schema.org\/url":[{"@value":"https:\/\/www.bco-dmo.org\/dataset\/639794","@type":"xsd:anyURI"}],"http:\/\/schema.org\/contentType":[{"@value":"text\/html","@type":"xsd:token"}]},"_:frictionlessdata639794":{"@id":"_:frictionlessdata639794","@type":["http:\/\/ocean-data.org\/schema\/MetadataDownloadAffordance"],"http:\/\/schema.org\/subjectOf":[{"@id":"http:\/\/lod.bco-dmo.org\/id\/dataset\/639794"}],"http:\/\/schema.org\/name":[{"@value":"Datapackage.json","@type":"xsd:string"}],"http:\/\/schema.org\/alternateName":[{"@value":"Frictionless Data Package","@type":"xsd:string"}],"http:\/\/ocean-data.org\/schema\/affordedBy":[{"@id":"http:\/\/lod.bco-dmo.org\/id\/affiliation\/191"}],"http:\/\/schema.org\/target":[{"@value":"_:frictionlessdata639794entryPoint"}]},"_:frictionlessdata639794entryPoint":{"@id":"_:frictionlessdata639794entryPoint","@type":["http:\/\/schema.org\/EntryPoint"],"http:\/\/schema.org\/url":[{"@value":"https:\/\/www.bco-dmo.org\/dataset\/639794\/datapackage.json","@type":"xsd:anyURI"}],"http:\/\/schema.org\/contentType":[{"@value":"application\/vnd.datapackage+json","@type":"xsd:token"}]},"_:datasetdescription639794":{"@id":"_:datasetdescription639794","@type":["http:\/\/ocean-data.org\/schema\/MetadataDownloadAffordance"],"http:\/\/schema.org\/subjectOf":[{"@id":"http:\/\/lod.bco-dmo.org\/id\/dataset\/639794"}],"http:\/\/schema.org\/name":[{"@value":"PDF","@type":"xsd:string"}],"http:\/\/ocean-data.org\/schema\/affordedBy":[{"@id":"http:\/\/lod.bco-dmo.org\/id\/affiliation\/191"}],"http:\/\/schema.org\/target":[{"@value":"_:datasetdescription639794entryPoint"}]},"_:datasetdescription639794entryPoint":{"@id":"_:datasetdescription639794entryPoint","@type":["http:\/\/schema.org\/EntryPoint","http:\/\/ocean-data.org\/schema\/PdfDatasetDescription"],"http:\/\/schema.org\/url":[{"@value":"https:\/\/www.bco-dmo.org\/dataset\/639794\/Dataset_description.pdf","@type":"xsd:anyURI"}],"http:\/\/schema.org\/contentType":[{"@value":"application\/pdf","@type":"xsd:token"}]},"_:json639794":{"@id":"_:json639794","@type":["http:\/\/ocean-data.org\/schema\/MetadataDownloadAffordance"],"http:\/\/schema.org\/subjectOf":[{"@id":"http:\/\/lod.bco-dmo.org\/id\/dataset\/639794"}],"http:\/\/schema.org\/name":[{"@value":"JSON-LD","@type":"xsd:string"}],"http:\/\/ocean-data.org\/schema\/affordedBy":[{"@id":"http:\/\/lod.bco-dmo.org\/id\/affiliation\/191"}],"http:\/\/schema.org\/target":[{"@value":"_:json639794entryPoint"}]},"_:json639794entryPoint":{"@id":"_:json639794entryPoint","@type":["http:\/\/schema.org\/EntryPoint"],"http:\/\/schema.org\/url":[{"@value":"https:\/\/www.bco-dmo.org\/dataset\/639794.json","@type":"xsd:anyURI"}],"http:\/\/schema.org\/contentType":[{"@value":"application\/ld+json","@type":"xsd:token"}]},"_:ttl639794":{"@id":"_:ttl639794","@type":["http:\/\/ocean-data.org\/schema\/MetadataDownloadAffordance"],"http:\/\/schema.org\/subjectOf":[{"@id":"http:\/\/lod.bco-dmo.org\/id\/dataset\/639794"}],"http:\/\/schema.org\/name":[{"@value":"Turtle","@type":"xsd:string"}],"http:\/\/ocean-data.org\/schema\/affordedBy":[{"@id":"http:\/\/lod.bco-dmo.org\/id\/affiliation\/191"}],"http:\/\/schema.org\/target":[{"@value":"_:ttl639794entryPoint"}]},"_:ttl639794entryPoint":{"@id":"_:ttl639794entryPoint","@type":["http:\/\/schema.org\/EntryPoint"],"http:\/\/schema.org\/url":[{"@value":"https:\/\/www.bco-dmo.org\/dataset\/639794.ttl","@type":"xsd:anyURI"}],"http:\/\/schema.org\/contentType":[{"@value":"text\/turtle","@type":"xsd:token"}]},"_:rdf639794":{"@id":"_:rdf639794","@type":["http:\/\/ocean-data.org\/schema\/MetadataDownloadAffordance"],"http:\/\/schema.org\/subjectOf":[{"@id":"http:\/\/lod.bco-dmo.org\/id\/dataset\/639794"}],"http:\/\/schema.org\/name":[{"@value":"RDF\/XML","@type":"xsd:string"}],"http:\/\/ocean-data.org\/schema\/affordedBy":[{"@id":"http:\/\/lod.bco-dmo.org\/id\/affiliation\/191"}],"http:\/\/schema.org\/target":[{"@value":"_:rdf639794entryPoint"}]},"_:rdf639794entryPoint":{"@id":"_:rdf639794entryPoint","@type":["http:\/\/schema.org\/EntryPoint"],"http:\/\/schema.org\/url":[{"@value":"https:\/\/www.bco-dmo.org\/dataset\/639794.rdf","@type":"xsd:anyURI"}],"http:\/\/schema.org\/contentType":[{"@value":"application\/rdf+xml","@type":"xsd:token"}]},"_:iso639794":{"@id":"_:iso639794","@type":["http:\/\/ocean-data.org\/schema\/MetadataDownloadAffordance"],"http:\/\/schema.org\/subjectOf":[{"@id":"http:\/\/lod.bco-dmo.org\/id\/dataset\/639794"}],"http:\/\/schema.org\/name":[{"@value":"ISO 19115-2 (NOAA Profile)","@type":"xsd:string"}],"http:\/\/ocean-data.org\/schema\/affordedBy":[{"@id":"http:\/\/lod.bco-dmo.org\/id\/affiliation\/191"}],"http:\/\/schema.org\/target":[{"@value":"_:iso639794entryPoint"}]},"_:iso639794entryPoint":{"@id":"_:iso639794entryPoint","@type":["http:\/\/schema.org\/EntryPoint","http:\/\/ocean-data.org\/schema\/ISOMetadata"],"http:\/\/schema.org\/url":[{"@value":"https:\/\/www.bco-dmo.org\/dataset\/639794\/iso","@type":"xsd:anyURI"}],"http:\/\/schema.org\/contentType":[{"@value":"application\/xml","@type":"xsd:token"}],"http:\/\/purl.org\/dc\/terms\/conformsTo":[{"@value":"http:\/\/www.isotc211.org\/2005\/gmd-noaa","@type":"xsd:anyURI"}]},"_:dublincore639794":{"@id":"_:dublincore639794","@type":["http:\/\/ocean-data.org\/schema\/MetadataDownloadAffordance"],"http:\/\/schema.org\/subjectOf":[{"@id":"http:\/\/lod.bco-dmo.org\/id\/dataset\/639794"}],"http:\/\/schema.org\/name":[{"@value":"Dublin Core","@type":"xsd:string"}],"http:\/\/ocean-data.org\/schema\/affordedBy":[{"@id":"http:\/\/lod.bco-dmo.org\/id\/affiliation\/191"}],"http:\/\/schema.org\/target":[{"@value":"_:dublincore639794entryPoint"}]},"_:dublincore639794entryPoint":{"@id":"_:dublincore639794entryPoint","@type":["http:\/\/schema.org\/EntryPoint"],"http:\/\/schema.org\/url":[{"@value":"https:\/\/www.bco-dmo.org\/dataset\/639794\/dublin-core","@type":"xsd:anyURI"}],"http:\/\/schema.org\/contentType":[{"@value":"application\/xml","@type":"xsd:token"}],"http:\/\/purl.org\/dc\/terms\/conformsTo":[{"@value":"http:\/\/purl.org\/dc\/elements\/1.1\/","@type":"xsd:anyURI"}]},"_:Identifier639794":{"@id":"_:Identifier639794","@type":["http:\/\/ocean-data.org\/schema\/Identifier","http:\/\/ocean-data.org\/schema\/BCODMOIdentifier","http:\/\/ocean-data.org\/schema\/OSPREY_v2_Node_dataset"],"http:\/\/ocean-data.org\/schema\/identifierScheme":[{"@id":"http:\/\/ocean-data.org\/schema\/IdentifierScheme_BCODMO_Version_2"}],"http:\/\/ocean-data.org\/schema\/identifierValue":[{"@value":"639794","@type":"xsd:token"}],"http:\/\/ocean-data.org\/schema\/resolvableURL":[{"@value":"http:\/\/lod.bco-dmo.org\/id\/dataset\/639794","@type":"xsd:anyURI"}]}}]}