<div><p>Temporal changes in biochemical, physiological and microbiological associations in the <em>Aplysina cauliformis </em>holobiont over the course of transmission of <em>Aplysina </em>Red Band Disease (ARBS) were investigated in two experiments, one performed in July and one performed in January. In both experiments, healthy and ARBS-affected sponges (n = 21-24 of each) were marked <em>in situ </em>at North Norman’s reef in the Exuma Cays, Bahamas. Healthy sponges were collected, labeled and a subsample (10 cm) was removed into an individual resealable plastic bag underwater to serve as an "initial" sample. The remainder of each of these healthy sponges was randomly attached to either a healthy <em>in situ</em> sample or to the active red band on a diseased <em>in situ </em>sample using a cable tie. In July, randomly selected pairs of healthy-healthy and healthy-diseased sponges were collected on days 3, 6 and 9 to provide "final" samples. In January, randomly selected pairs of each type were collected on days 1, 3 and 9. Following final collection, all pairs were separated in the lab, and photographs were taken to confirm whether ARBS transmission had occurred.</p>
<p>Following collection of initial and final samples, the sponges were subsampled for several analyses. Only sections of healthy tissue were used, even from the ARBS-affected sponges (Gochfeld et al. 2012, Marine Ecology Progress Series; Gochfeld et al. 2012, Journal of Chemical Ecology; Olson et al. 2014). Subsamples were collected for measurement of chlorophyll a, total protein, secondary metabolite profiles, and microbial community analysis. In addition, in January, subsamples were also collected for heat shock protein 70 expression analysis.</p>
<p>Analytical methods followed those of Gochfeld et al. (2012, Marine Ecology Press Series) for chlorophyll <em>a </em>and total protein, and Olson et al. (2014) for microbial community assemblages. Secondary metabolite profiles used similar methods to those described in Gochfeld et al. (2012, Journal of Chemical Ecology) except that the samples were extracted three times in methanol prior to generating chemical profiles using HPLC. Areas under the curve for peaks that were consistently found in either <em>in situ</em> healthy and/or <em>in situ </em>diseased sponges were quantified. Heat shock protein 70 expression analysis was performed following methods in Sarkis et al. (2005).</p></div>
Heat shock protein 70 (HSP70) data from study of sponge disease transmission.
<div><p>Heat shock protein 70 (HSP70) data from study of sponge disease transmission. Temporal changes in biochemical, physiological and microbiological associations in the <em>Aplysina cauliformis</em> holobiont over the course of transmission of <em>Aplysina</em> Red Band Disease (ARBS) were investigated in two experiments, one performed in July and one performed in January.</p>
<p><strong>Related datasets:</strong><br /><a href="http://www.bco-dmo.org/dataset/640933" target="_blank">Sponge Disease Transmission - Chl-a</a><br /><a href="http://www.bco-dmo.org/dataset/641052" target="_blank">Sponge Disease Transmission - Secondary Metabolites</a><br /><a href="https://www.bco-dmo.org/dataset/749722" target="_blank">Sponge Disease Transmission - Total Protein</a><br /><a href="https://www.bco-dmo.org/dataset/749753" target="_blank">Sponge Disease Transmission - T-REX</a>
</p></div>
Sponge Disease Transmission - HSP70
<div><p>Chlorophyll-a<em> </em>and protein concentrations, areas under the curve of secondary metabolite peaks, and HSP70 band intensity from healthy sponges attached to either healthy or diseased <em>in situ </em>sponges were compared using repeated measures ANCOVA, with time (initial vs. final) and treatment (attached to a healthy or diseased sponge) as the main factors, and the number of days of attachment (3, 6, 9 in July; 1, 3, 9 in January) as the covariate. For the <em>in situ</em> sponges from which only final samples were collected, parameters were compared using a one-way ANCOVA with treatment (initially healthy or diseased) as the main factor and day collected (equivalent to number of days of attachment: 3, 6, 9 in July; 1, 3, 9 in January) as the covariate. Microbial community assemblage data were analyzed using T-REX and PRIMER, as described in Olson et al. (2014).</p>
<p>BCO-DMO Processing:<br />
- Reorganized data into one table (rather than 2);<br />
- Added "sample_type" column;<br />
- Added location information provided on metadata form, converted lat/lon to decimal degrees.<br />
- 16-Nov-2018: removed embargo on dataset.<br /></p></div>
641090
Sponge Disease Transmission - HSP70
2016-03-18T14:45:28-04:00
2016-03-18T14:45:28-04:00
2023-07-07T16:10:26-04:00
urn:bcodmo:dataset:641090
Heat shock protein 70 (HSP70) data from study of sponge disease transmission in the Exuma Cays, Bahamas from 2009-2014 (Sponge Disease Model project)
false
Gochfeld, D. J., Brandt, M., Olson, J. (2016) Heat shock protein 70 (HSP70) data from study of sponge disease transmission in the Exuma Cays, Bahamas from 2009-2014 (Sponge Disease Model project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 17 March 2016) Version Date 2016-03-17 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/641090 [access date]
false
17 March 2016
false
2016-03-17
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