http://lod.bco-dmo.org/id/dataset/641654
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2016-04-01
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Results of stable isotope analysis (C, N) of the posteriorly regenerated muscle tissue of Diopatra from the Georgia and South Carolina coasts in 2013 (Gracilaria effects project)
2016-04-07
publication
2016-04-07
revision
BCO-DMO Linked Data URI
2016-04-07
creation
http://lod.bco-dmo.org/id/dataset/641654
James E. Byers
University of Georgia
principalInvestigator
Erik Sotka
College of Charleston
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Sotka, E., Byers, J. E. (2016) Results of stable isotope analysis (C, N) of the posteriorly regenerated muscle tissue of Diopatra from the Georgia and South Carolina coasts in 2013 (Gracilaria effects project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). Version Date 2016-04-07 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/641654 [access date]
Data for Kollars etal (2016) MEPS Figure 4B: Results of stable isotope analysis (C, N) of the posteriorly regenerated muscle tissue of Diopatra Dataset Description: Methods and Sampling: <p><strong>Lab Experiments: </strong>Using laboratory and field-based experiments, we tested whether Gracilaria increased the survivorship and growth of Diopatra through direct or indirect provisioning of food, or through refuge from predation. Diopatra tubes extend up to 1 m below the benthos and it is difficult to collect Diopatra bodies without incidentally severing the worm. Severing, however, does not cause mortality because these animals are capable of both anterior and posterior regeneration (Berke et al. 2009). To standardize the initial size of the worm and create a point of posterior regeneration, we cut the field-collected worms to ~3 cm in length. We buried the worm in field-collected sediment contained in a 15 cm long × 3 cm diameter plastic tube in the laboratory. Tubes were held in racks in a recirculating seawater table at 22°C and a salinity of 30 ppt to allow the worms to regenerate their sediment-based tubes before experimentation, which usually happened within 24 h (Berke et al. 2009, N. M. Kollars pers. obs.).</p>
<p>In the laboratory, we offered Gracilaria-associated diet items to Diopatra and measured survivorship and growth after 6 wk. Diopatra were collected from the Fort Johnson mudflat in January 2013, and placed into plastic tubes that were encircled with window screen to create feeding chambers that still allowed water flow. Diopatra were randomly offered one of 4 diets: sediment-only control, Gracilaria, amphipods, or Gracilaria and amphipods (n = 24 per treatment). Gracilaria was offered ad libitum and replaced weekly. Three frozen, field-collected amphipods were offered daily (primarily Gammarus mucronatus, the most abundant amphipod species on Gracilaria; Wright et al. 2014). Though we offered Diopatra dead amphipods due to logistical constraints, we do have video evidence that shows that, in the laboratory, Diopatra are capable of pursuing and catching amphipods (see video Supplement 2 at www. int-res. com/ articles/ suppl/ m545 p135_ supp/). After 6 wk, we removed Diopatra from their sediment tubes and dried them at 60°C until no change in mass occurred and measured the final body mass. To examine the effects of diet treatment on Diopatra final dry weight, we used a 1-way ANOVA followed by a post-hoc Tukey’s test.</p>
<p>To assess the incorporation of the supplied food source into the new worm tissue, we quantified the carbon and nitrogen stable isotopic signatures in the posteriorly regenerated tissue of 5 worms from each diet treatment. We randomly selected 5 individuals from each diet treatment and sampled the dried posteriorly regenerated tissue (targeting muscle tissue and avoiding the digestive tract or fecal pellets). For comparison, we also analyzed 3 samples of each resource (collected from the field in April 2013): Gracilaria and amphipods. We generated the isotopic signature data using a Delta V plus spectrometer (ThermoFinnigan), with a Thermo Flash EA as the interface at the Skidaway Institute Scientific Stable Isotope Laboratory (Savannah, GA, USA).</p>
<p><strong>Related Reference:</strong><br />
Kollars, N.M., J.E. Byers and E.E. Sotka (2016) Invasive decor: an association between a native decorator worm and a non-native seaweed can be mutualistic. Marine Ecology Progress Series (DOI: 10.3354/meps11602)</p>
<p><strong>Related Datasets:</strong><br />
<a href="http://www.bco-dmo.org/dataset/641577">MEPS_2016: Fig.2A - survey</a><br />
<a href="http://www.bco-dmo.org/dataset/641615">MEPS_2016: Fig.2B - Gracilaria growth rate</a><br />
<a href="http://www.bco-dmo.org/dataset/641628">MEPS_2016: Fig.3 - growth rate and depth</a><br />
<a href="http://www.bco-dmo.org/dataset/641641">MEPS_2016: Fig.4A - worm growth</a><br />
<a href="http://www.bco-dmo.org/dataset/641667">MEPS_2016: Fig.5A - field expt 2012</a><br />
<a href="http://www.bco-dmo.org/dataset/641680">MEPS_2016: Fig.5B - field expt 2013</a></p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1057707 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1057707
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1057713 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1057713
completed
James E. Byers
University of Georgia
706-583-0012
Odum School of Ecology 140 E. Green St.
Athens
GA
30602
USA
jebyers@uga.edu
pointOfContact
Erik Sotka
College of Charleston
843-953-9191
Grice Marine Laboratory 205 Fort Johnson Road
Charleston
SC
29412
USA
SotkaE@cofc.edu
pointOfContact
asNeeded
Unknown
sample
treatment
N_pcent
N15
C_pcent
C13
theme
None, User defined
sample identification
treatment
No BCO-DMO term
biomass_C
featureType
BCO-DMO Standard Parameters
Elemental Analyzer
Spectrometer
instrument
BCO-DMO Standard Instruments
Sotka_2013
service
Deployment Activity
Georgia and South Carolina coasts
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Cascading effects of an invasive seaweed on estuarine food webs of the southeastern US
https://osprey.bco-dmo.org/project/564687
Cascading effects of an invasive seaweed on estuarine food webs of the southeastern US
<p><em>Description from NSF award abstract:</em><br />
During the last decade, the Asian seaweed, <em>Gracilaria vermiculophylla</em>, has proliferated along high-salinity mudflats in several Georgia and South Carolina estuaries. The invasion is noteworthy because the mudflats in these estuaries were historically devoid of macrophyte-based primary production and structure. <em>Gracilaria</em> has few native analogues in these mudflat environments, and thus represents an opportunity to examine the ecosystem consequences of an invasion within an historically-unexploited niche. In theory, <em>Gracilaria</em> affects populations of species that are directly dependent on the invader for structure and food, as well as altering community- and ecosystem-level processes such as detrital production and food web structure. Through a combination of manipulative field experiments, laboratory assays and stable isotope analysis, the investigators will test three mechanisms by which <em>Gracilaria</em> influences native community structure. The novel structure and primary production generated by <em>Gracilaria vermiculophylla</em> may be 1) increasing rates of secondary production, 2) increasing levels of mudflat microbial production through leeching of dissolved nutrients, and 3) increasing detrital input to microbial and macrobial food webs.</p>
<p>This project will provide a mechanistic understanding of the multiple cascading impacts of an invasive species within the estuarine community. Species invasions that alter ecosystem functions are usually the most profound. These alterations are often generated by a small number of invaders that create physical structure, including important biogenic habitat, de novo. By altering physical structure, these non-native ecosystem engineers alter local abiotic conditions, interactions between species, and species composition. Highly influential invaders may also change food web structure and trophic flow of energy and materials. Such substantive food web changes can occur when an influential invader provides nutrients or resources that are different in quality, quantity or both. An invasive species that both provisions new physical structure and fundamentally alters food web structure could exert an overwhelming influence on native communities when these mechanisms act in synergy.</p>
Gracilaria effects
largerWorkCitation
project
eng; USA
biota
oceans
Georgia and South Carolina coasts
-79.89816
-79.89816
32.75196
32.75196
2016-04-07
Georgia and South Carolina coasts
0
BCO-DMO catalogue of parameters from Results of stable isotope analysis (C, N) of the posteriorly regenerated muscle tissue of Diopatra from the Georgia and South Carolina coasts in 2013 (Gracilaria effects project)
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/642026.rdf
Name: sample
Units: unitless
Description: <p>unique identification number for each sampled (Diopatra?) worm</p>
http://lod.bco-dmo.org/id/dataset-parameter/642027.rdf
Name: treatment
Units: unitless
Description: <p>treatment assignment: mud=sediment-only control; grac=Gracilaria provided ad libitum; pods=provided 3 freshly-killed and frozen amphipods [primarily Gammarus mucranatus] daily; grac_and_pods=Gracilaria provided ad libitum and provided with 3 freshly killed and frozen amphipods; pods_field=additional resource samples that were collected from the field</p>
http://lod.bco-dmo.org/id/dataset-parameter/642028.rdf
Name: N_pcent
Units: percent
Description: <p>percent nitrogen content</p>
http://lod.bco-dmo.org/id/dataset-parameter/642029.rdf
Name: N15
Units: percent
Description: <p>nitrogen as 15N content</p>
http://lod.bco-dmo.org/id/dataset-parameter/642030.rdf
Name: C_pcent
Units: percent
Description: <p>percent carbon content</p>
http://lod.bco-dmo.org/id/dataset-parameter/642031.rdf
Name: C13
Units: percent
Description: <p>carbon as 13C content</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
1122
https://datadocs.bco-dmo.org/file/N77GrZwIPJqpGx/Kollars_fig4B.csv
Kollars_fig4B.csv
Primary data file for dataset ID 641654
download
https://osprey.bco-dmo.org/dataset/641654/data/download
download
onLine
dataset
<p><strong>Lab Experiments: </strong>Using laboratory and field-based experiments, we tested whether Gracilaria increased the survivorship and growth of Diopatra through direct or indirect provisioning of food, or through refuge from predation. Diopatra tubes extend up to 1 m below the benthos and it is difficult to collect Diopatra bodies without incidentally severing the worm. Severing, however, does not cause mortality because these animals are capable of both anterior and posterior regeneration (Berke et al. 2009). To standardize the initial size of the worm and create a point of posterior regeneration, we cut the field-collected worms to ~3 cm in length. We buried the worm in field-collected sediment contained in a 15 cm long × 3 cm diameter plastic tube in the laboratory. Tubes were held in racks in a recirculating seawater table at 22°C and a salinity of 30 ppt to allow the worms to regenerate their sediment-based tubes before experimentation, which usually happened within 24 h (Berke et al. 2009, N. M. Kollars pers. obs.).</p>
<p>In the laboratory, we offered Gracilaria-associated diet items to Diopatra and measured survivorship and growth after 6 wk. Diopatra were collected from the Fort Johnson mudflat in January 2013, and placed into plastic tubes that were encircled with window screen to create feeding chambers that still allowed water flow. Diopatra were randomly offered one of 4 diets: sediment-only control, Gracilaria, amphipods, or Gracilaria and amphipods (n = 24 per treatment). Gracilaria was offered ad libitum and replaced weekly. Three frozen, field-collected amphipods were offered daily (primarily Gammarus mucronatus, the most abundant amphipod species on Gracilaria; Wright et al. 2014). Though we offered Diopatra dead amphipods due to logistical constraints, we do have video evidence that shows that, in the laboratory, Diopatra are capable of pursuing and catching amphipods (see video Supplement 2 at www. int-res. com/ articles/ suppl/ m545 p135_ supp/). After 6 wk, we removed Diopatra from their sediment tubes and dried them at 60°C until no change in mass occurred and measured the final body mass. To examine the effects of diet treatment on Diopatra final dry weight, we used a 1-way ANOVA followed by a post-hoc Tukey’s test.</p>
<p>To assess the incorporation of the supplied food source into the new worm tissue, we quantified the carbon and nitrogen stable isotopic signatures in the posteriorly regenerated tissue of 5 worms from each diet treatment. We randomly selected 5 individuals from each diet treatment and sampled the dried posteriorly regenerated tissue (targeting muscle tissue and avoiding the digestive tract or fecal pellets). For comparison, we also analyzed 3 samples of each resource (collected from the field in April 2013): Gracilaria and amphipods. We generated the isotopic signature data using a Delta V plus spectrometer (ThermoFinnigan), with a Thermo Flash EA as the interface at the Skidaway Institute Scientific Stable Isotope Laboratory (Savannah, GA, USA).</p>
<p><strong>Related Reference:</strong><br />
Kollars, N.M., J.E. Byers and E.E. Sotka (2016) Invasive decor: an association between a native decorator worm and a non-native seaweed can be mutualistic. Marine Ecology Progress Series (DOI: 10.3354/meps11602)</p>
<p><strong>Related Datasets:</strong><br />
<a href="http://www.bco-dmo.org/dataset/641577">MEPS_2016: Fig.2A - survey</a><br />
<a href="http://www.bco-dmo.org/dataset/641615">MEPS_2016: Fig.2B - Gracilaria growth rate</a><br />
<a href="http://www.bco-dmo.org/dataset/641628">MEPS_2016: Fig.3 - growth rate and depth</a><br />
<a href="http://www.bco-dmo.org/dataset/641641">MEPS_2016: Fig.4A - worm growth</a><br />
<a href="http://www.bco-dmo.org/dataset/641667">MEPS_2016: Fig.5A - field expt 2012</a><br />
<a href="http://www.bco-dmo.org/dataset/641680">MEPS_2016: Fig.5B - field expt 2013</a></p>
Specified by the Principal Investigator(s)
<p>&nbsp;</p>
<p><strong>BCO-DMO Processing:</strong><br />
- added conventional header with dataset name, PI name, version date, reference information<br />
- renamed parameters to BCO-DMO standard<br />
- replaced spaces with underscores<br />
- replaced special characters</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
PI Supplied Instrument Name: PI Supplied Instrument Description:Thermo Flash EA Instrument Name: Elemental Analyzer Instrument Short Name: Instrument Description: Instruments that quantify carbon, nitrogen and sometimes other elements by combusting the sample at very high temperature and assaying the resulting gaseous oxides. Usually used for samples including organic material. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB01/
PI Supplied Instrument Name: PI Supplied Instrument Description:Delta V plus spectrometer Instrument Name: Spectrometer Instrument Short Name:Spectrometer Instrument Description: A spectrometer is an optical instrument used to measure properties of light over a specific portion of the electromagnetic spectrum. Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0460/
Deployment: Sotka_2013
Sotka_2013
Coll_Charleston
shoreside
Sotka_2013
Erik Sotka
College of Charleston
Coll_Charleston
shoreside