<div><p><strong>Field experiments</strong><br />
To quantify the effect of Gracilaria on Diopatra growth and survivorship in the field, we manipulated Gracilaria density and predator access to Diopatra in a fully factorial and blocked field experiment over 4 wk in June 2012. We prepared worms in plastic tubes as above and out-planted them along a transect parallel to the shore at ~+0.15 m MLLW in one of 4 treatments: ambient control, Gracilaria addition, Gracilaria addition and predator exclusion cage, and cage only. Two worms were added per plot and 10 plots were generated per treatment. We report the final body masses of surviving worms (if both survived we used the average of the 2 worms). Treatments of a given replicate block were within 1 m of each other and blocks were separated by at least 1 m. We added Gracilaria to a plot by weaving 50.00 g (± 0.05 g, acceptable range of variation) blotted wet mass of the seaweed through 30 cm of 0.76 cm 3- strand rope and placing the rope in the plot center. A pre-experimental survey indicated that the maximum seaweed biomass on a Diopatra individual in the field was ~25.0 g wet mass (data not shown). Cages were 0.5 m wide by 0.3 m tall, constructed with PVC-coated chicken wire (2.5 cm mesh size) and embedded ~10 cm into the sediment. Plots without cages were marked with 3 PVC posts extending <10 cm out of the sediment and separated by ~30 cm. After 4 wk, we removed the worms and severed the regenerated tissue from the rest of the body (see photo graph in Supplement 1B at <a href="http://www.int-res.com/articles/suppl/m545p135_supp/">http://www.int-res.com/articles/suppl/m545p135_supp/</a>) using a razor blade. We dried and measured the original body mass and regenerated tissue mass at 60°C until no change in mass occurred (see Supplement 1B for details). We collected all Gracilaria biomass within the plot, defaunated it, measured its wet mass and dry mass, and counted the number of whole, epifaunal amphipods.</p>
<p>To assess spatial and temporal variation in the effect of Gracilaria on Diopatra, we manipulated Gracilaria density in replicated field experiments in June 2013 at 2 sites near Charleston, SC (Fort Johnson, Stono River) and 2 sites near Savannah, GA (Priest’s Landing and Bull’s River). The 2013 experiments did not include a cage treatment be cause we did not detect a predator-exclusion effect in 2012 (see ‘Results’). Relative to the 2012 experiment, the 2013 experiments used only one worm per plot, had 30 replicates per treatment, and we reduced the initial level of Gracilaria biomass in the addition treatment from 50 g down to 25 g wet mass. We randomly distributed plots 1 m apart along a transect parallel to the shore at approx. +0.09 m MLLW. In addition, we attached the seaweed-embedded ropes to PVC posts using zip-ties and pushed the posts into the mudflat to in crease seaweed stability (see Supplement 1A). Plots where we did not add Gracilaria still contained a PVC post and empty rope. After 4 wk, we processed the worms, seaweed, and epifauna as in the 2012 experiment. Because of logistical constraints, we counted epifauna for only 2 of the 4 sites (Fort Johnson and Priest’s Landing) used in 2013.</p>
<p><strong>Related Reference:</strong><br />
Kollars, N.M., J.E. Byers and E.E. Sotka (2016) Invasive decor: an association between a native decorator worm and a non-native seaweed can be mutualistic. Marine Ecology Progress Series (DOI: 10.3354/meps11602)</p>
<p><strong>Related Datasets:</strong><br /><a href="http://www.bco-dmo.org/dataset/641577">MEPS_2016: Fig.2A - survey</a><br /><a href="http://www.bco-dmo.org/dataset/641615">MEPS_2016: Fig.2B - Gracilaria growth rate</a><br /><a href="http://www.bco-dmo.org/dataset/641628">MEPS_2016: Fig.3 - growth rate and depth</a><br /><a href="http://www.bco-dmo.org/dataset/641641">MEPS_2016: Fig.4A - worm growth</a><br /><a href="http://www.bco-dmo.org/dataset/641654">MEPS_2016: Fig.4B - stable isotopes</a><br /><a href="http://www.bco-dmo.org/dataset/641680">MEPS_2016: Fig.5B - field expt 2013</a></p></div>
Data for Kollars etal (2016) MEPS Figure 5A: Response of Diopatra final size to Gracilaria abundance and the predator-exclusion cages at Fort Johnson, Charleston SC in 2012
MEPS_2016: Fig.5A - field expt 2012
<div><p>We analyzed survivorship of Diopatra as a function of treatment (Gracilaria addition and presence or absence of cage [2012 only]) using a Chi<sup>2</sup> test. Because we did not measure Gracilaria final density for treatments in which worms died, we cannot pursue a logistic regression. For growth analyses, we excluded worms with evidence of sublethal predation on Diopatra (c.f. Berke et al. 2009; seen only in 2013 and in GA; 5 of 239 worms removed) and worms that were damaged during collection and processing (2 damaged worms out of 80 in June 2012; 15 damaged worms out of 239 in June 2013). Although the Gracilaria manipulation was initially designed as a categorical variable, we treated Gracilaria final biomass as an independent, continuous variable in our growth analysis because plots gained or lost Gracilaria biomass from Diopatra decoration activity, Gracilaria growth, and Gracilaria detachment. Moreover, epifauna abundance - an important proxy for Diopatra growth in the laboratory growth assay - scales positively with Gracilaria abundance (Supplement 1C at <a href="http://www.int-res.com/articles/suppl/m545p135_supp/" target="_blank">http://www.int-res.com/articles/suppl/m545p135_supp/</a>, Byers et al. 2012). The response of Diopatra final size (as well as other growth metrics, Supplement 1B) was analyzed using ANCOVA with Gracilaria density as a covariate and cage presence as a categorical variable for the 2012 experiment, and Gracilaria density as a covariate and site as a categorical variable for the 2013 experiment. To assess the potential for Gracilaria as an indirect food source in the field (i.e. an attractor of prey species for Diopatra), we examined the relationship between counts of epifaunal amphipods and Gracilaria biomass using a generalized linear model with a quasi-Poisson distribution (Zuur et al. 2009). Finally, we used ANCOVA to examine the relationship between Diopatra size and the count of epifaunal amphipods.</p>
<p><strong>BCO-DMO Processing:</strong><br />
- added conventional header with dataset name, PI name, version date, reference information<br />
- renamed parameters to BCO-DMO standard<br />
- added site, lat and lon columns for mapping purposes</p></div>
641667
MEPS_2016: Fig.5A - field expt 2012
2016-04-01T14:56:39-04:00
2016-04-01T14:56:39-04:00
2023-07-07T16:10:26-04:00
urn:bcodmo:dataset:641667
Response of Diopatra final size to Gracilaria abundance and the predator-exclusion cages in Fort Johnson, Charleston South Carolina from 2012 (Gracilaria effects project)
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Sotka, E., Byers, J. E. (2016) Response of Diopatra final size to Gracilaria abundance and the predator-exclusion cages in Fort Johnson, Charleston South Carolina from 2012 (Gracilaria effects project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). Version Date 2016-04-07 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/641667 [access date]
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