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            <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/dataset/641759.rdf" xlink:actuate="onRequest">Tank seawater conditions from Coral/Temperature/pCO2 Experiments at LTER site in Moorea, French Polynesia, 2011 (OA_Corals project)</gmx:Anchor>
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            <gco:CharacterString>Cite this dataset as: Edmunds, P. (2016) Tank seawater conditions from Coral/Temperature/pCO2 Experiments at LTER site in Moorea, French Polynesia, 2011 (OA_Corals project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). Version Date 2016-04-04 [if applicable, indicate subset used]. doi:10.1575/1912/bco-dmo.641942 [access date]</gco:CharacterString>
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        <gco:CharacterString>Seawater conditions in 8 tanks Dataset Description: &amp;lt;p&amp;gt;Summary of conditions in the eight tanks assigned randomly to create four treatments of AT-ACO2.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;Related Reference:&amp;lt;/strong&amp;gt;&amp;lt;br /&amp;gt;
Darren Brown, Peter J. Edmunds. Differences in the responses of three scleractinians and the hydrocoral Millepora platyphylla to ocean acidification. Marine Biology, 2016 (in press).&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;Related Dataset:&amp;lt;/strong&amp;gt;&amp;lt;br /&amp;gt;
&amp;lt;a href=&amp;quot;http://www.bco-dmo.org/dataset/641479&amp;quot; target=&amp;quot;_blank&amp;quot;&amp;gt;MarBio. 2016: calcification and biomass&amp;lt;/a&amp;gt;&amp;lt;/p&amp;gt; Methods and Sampling: &amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;Experimental conditions and maintenance&amp;lt;/strong&amp;gt;&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Treatments were created in 8 tanks (Aqua Logic, San Diego), each holding 150 L of seawater and regulated independently for temperature, light, and pCO2.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Temperatures were maintained at 28.0°C, which corresponded to the ambient seawater temperature in the back reef when the study was conducted, and 30.1°C which is close to the maximum temperature in this habitat (Putnam and Edmunds 2011). pCO2 treatments contrasted ambient conditions (~ 408 micro-atm) and 913 micro-atm pCO2, with the elevated value expected to occur within 100 y under the &amp;quot;stabilization without overshoot&amp;quot; representative concentration pathway (RCP 6.0) (van Vuuren et al. 2011). pCO2 treatments were created by bubbling ambient air or a mixture of ambient air and pure CO2 that was blended continually and monitored using an infrared gas analyzer (IRGA model S151, Qubit Systems). A solenoid-controlled, gas regulation system (Model A352, Qubit Systems, Ontario, Canada) regulated the flow of CO2 and air, with pCO2 logged on a PC running LabPro software (Vemier Software and Technology). Ambient air and the elevated pCO2 mixture were supplied at ~ 10-15 L min-1 to treatment tanks using pumps (Gast pump DOA-P704-AA, see Edmunds 2011).&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;The temperatures and pCO2 levels created four treatments with two tanks treatment-1: ambient temperature-ambient pCO2 (AT-ACO2), ambient temperature-high pCO2 (AT-HCO2), high temperature-ambient pCO2 (HT-ACO2) and high temperature-high pCO2 (HT-HCO2). Treatment conditions were monitored daily, with temperature measured at 08:00, 12:00 and 18:00 hrs using a digital thermometer (Fisher Scientific model #150778, ± 0.05 °C), and light intensities at 12:00 hrs using a Li-Cor LI-193 sensor attached t 170 o a LI-1400 meter. Seawater within each tank was replaced at 200 ml/min with filtered seawater (50 micro-m) pumped from Cook’s Bay.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;Carbonate chemistry and pH analysis&amp;lt;/strong&amp;gt;&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;To evaluate dissolved inorganic carbon (DIC) conditions in the 8 tanks, total alkalinity (TA) and pH of the seawater were recorded every third day of the experiment. Seawater was collected between 07:00-09:00 hrs using stoppered glass bottles, equilibrated to room temperature (25.0°C), and processed within 2-3 hrs of collection. TA was determined using an open cell potentiometric titrator (Model T50, Mettler-Toledo, Columbus, OH) fitted with a DG115-SC pH probe (Mettler-Toledo, Columbus, OH) calibrated daily using NBS buffers (pH 4.00, 7.00 and 10.00, Fisher Scientific, 15-0787-8, ± 0.05 °C), and used to perform gran titrations using standard operating procedure 3 (SOP) of Dickson et al. (2007). Seawater pH was determined spectrophotometrically using the dye m-cresol purple (SOP 6b of Dickson et al. 2007), where pH was expressed on the total scale. The results of the gran titrations together with seawater salinity (YSI 3100 conductivity meter) and seawater temperature were used to calculate TA, pCO2, HCO3-, CO3 2- and aragonite saturation state (Omega) using CO2SYS (Lewis and Wallace 1998), with the constants of Mehrbach et al. (1973) and pH on the total scale.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;To evaluate the accuracy and precision of TA analyses, certified reference materials (CRM, batch 105 from A. Dickson, Scripps Institution of Oceanography) were processed before each set of seawater samples. CRMs were evaluated with a mean error of 0.37% (~ 8 micro-mol kg-1, n = 11) relative to the certified values. The precision and accuracy of pH measurements were evaluated using standardized Tris buffers (Batch 5 from A. D 193 Dickson Laboratory, Scripps Institution of Oceanography) that were processed spectrophotometrically with m-cresol as described above. Percent average error from the known pH of the Tris buffer was 0.16% (0.01 pH units, n = 13).&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;Incubation schedule and dependent variables&amp;lt;/strong&amp;gt;&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;On April 24th 2011, nubbins and cores were buoyant weighed (± 1 mg, Spencer-Davies 1989) and randomly placed in the mesocosm, with four taxa and two replicates per taxon in each tank. Over the following 24 h, seawater temperature and pCO2 were adjusted to target values. Corals remained in the treatments for 19 d, and were moved randomly within the tanks daily to eliminate position effects. Individual corals, along with the racks holding them, were cleaned every 5 d by wiping algal growth from walls of the tanks, racks, and PVC coral holders. On May 12th, the experiment ended and the corals were again buoyant weighed and the area of living tissue determined using aluminum foil (Marsh 1970).&amp;lt;/p&amp;gt;</gco:CharacterString>
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        <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/award/54987.rdf" xlink:title="OCE-0417412" xlink:actuate="onRequest">Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-0417412 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=0417412</gmx:Anchor>
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        <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/award/55110.rdf" xlink:title="OCE-1041270" xlink:actuate="onRequest">Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1041270 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1041270</gmx:Anchor>
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        <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/award/520630.rdf" xlink:title="OCE-1026851" xlink:actuate="onRequest">Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1026851 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1026851</gmx:Anchor>
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                            <gco:CharacterString>NSF Climate Research Investment (CRI) activities that were initiated in 2010 are now included under Science, Engineering and Education for Sustainability NSF-Wide Investment (SEES). SEES is a portfolio of activities that highlights NSF's unique role in helping society address the challenge(s) of achieving sustainability. Detailed information about the SEES program is available from NSF (https://www.nsf.gov/funding/pgm_summ.jsp?pims_id=504707).
In recognition of the need for basic research concerning the nature, extent and impact of ocean acidification on oceanic environments in the past, present and future, the goal of the SEES: OA program is to understand (a) the chemistry and physical chemistry of ocean acidification; (b) how ocean acidification interacts with processes at the organismal level; and (c) how the earth system history informs our understanding of the effects of ocean acidification on the present day and future ocean.
Solicitations issued under this program:
NSF 10-530, FY 2010-FY2011
NSF 12-500, FY 2012
NSF 12-600, FY 2013
NSF 13-586, FY 2014
NSF 13-586 was the final solicitation that will be released for this program.
PI Meetings:
1st U.S. Ocean Acidification PI Meeting(March 22-24, 2011, Woods Hole, MA)
2nd U.S. Ocean Acidification PI Meeting(Sept. 18-20, 2013, Washington, DC)
3rd U.S. Ocean Acidification PI Meeting (June 9-11, 2015, Woods Hole, MA – Tentative)
NSF media releases for the Ocean Acidification Program:
Press Release 10-186 NSF Awards Grants to Study Effects of Ocean Acidification
Discovery Blue Mussels &quot;Hang On&quot; Along Rocky Shores: For How Long?
Discovery nsf.gov - National Science Foundation (NSF) Discoveries - Trouble in Paradise: Ocean Acidification This Way Comes - US National Science Foundation (NSF)
Press Release 12-179 nsf.gov - National Science Foundation (NSF) News - Ocean Acidification: Finding New Answers Through National Science Foundation Research Grants - US National Science Foundation (NSF)
Press Release 13-102 World Oceans Month Brings Mixed News for Oysters
Press Release 13-108 nsf.gov - National Science Foundation (NSF) News - Natural Underwater Springs Show How Coral Reefs Respond to Ocean Acidification - US National Science Foundation (NSF)
Press Release 13-148 Ocean acidification: Making new discoveries through National Science Foundation research grants
Press Release 13-148 - Video nsf.gov - News - Video - NSF Ocean Sciences Division Director David Conover answers questions about ocean acidification. - US National Science Foundation (NSF)
Press Release 14-010 nsf.gov - National Science Foundation (NSF) News - Palau's coral reefs surprisingly resistant to ocean acidification - US National Science Foundation (NSF)
Press Release 14-116 nsf.gov - National Science Foundation (NSF) News - Ocean Acidification: NSF awards $11.4 million in new grants to study effects on marine ecosystems - US National Science Foundation (NSF)</gco:CharacterString>
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                            <gco:CharacterString>&lt;p&gt;While coral reefs have undergone unprecedented changes in community structure in the past 50 y, they now may be exposed to their gravest threat since the Triassic. This threat is increasing atmospheric CO2, which equilibrates with seawater and causes ocean acidification (OA). In the marine environment, the resulting decline in carbonate saturation state (Omega) makes it energetically less feasible for calcifying taxa to mineralize; this is a major concern for coral reefs. It is possible that the scleractinian architects of reefs will cease to exist as a mineralized taxon within a century, and that calcifying algae will be severely impaired. While there is a rush to understand these effects and make recommendations leading to their mitigation, these efforts are influenced strongly by the notion that the impacts of pCO2 (which causes Omega to change) on calcifying taxa, and the mechanisms that drive them, are well-known. The investigators believe that many of the key processes of mineralization on reefs that are potentially affected by OA are only poorly known and that current knowledge is inadequate to support the scaling of OA effects to the community level. It is vital to measure organismal-scale calcification of key taxa, elucidate the mechanistic bases of these responses, evaluate community scale calcification, and finally, to conduct focused experiments to describe the functional relationships between these scales of mineralization.&lt;/p&gt;
&lt;p&gt;This project is a 4-y effort focused on the effects of Ocean Acidification (OA) on coral reefs at multiple spatial and functional scales. The project focuses on the corals, calcified algae, and coral reefs of Moorea, French Polynesia, establishes baseline community-wide calcification data for the detection of OA effects on a decadal-scale, and builds on the research context and climate change focus of the Moorea Coral Reef LTER.&lt;/p&gt;
&lt;p&gt;This project is a hypothesis-driven approach to compare the effects of OA on reef taxa and coral reefs in Moorea. The PIs will utilize microcosms to address the impacts and mechanisms of OA on biological processes, as well as the ecological processes shaping community structure. Additionally, studies of reef-wide metabolism will be used to evaluate the impacts of OA on intact reef ecosystems, to provide a context within which the experimental investigations can be scaled to the real world, and critically, to provide a much needed reference against which future changes can be gauged.&lt;/p&gt;
&lt;p&gt;&lt;strong&gt;Datasets listed in the &quot;Dataset Collection&quot; section include references to results journal publications published as part of this project.&lt;/strong&gt;&lt;/p&gt;</gco:CharacterString>
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&amp;lt;p&amp;gt;Temperatures were maintained at 28.0°C, which corresponded to the ambient seawater temperature in the back reef when the study was conducted, and 30.1°C which is close to the maximum temperature in this habitat (Putnam and Edmunds 2011). pCO2 treatments contrasted ambient conditions (~ 408 micro-atm) and 913 micro-atm pCO2, with the elevated value expected to occur within 100 y under the &amp;quot;stabilization without overshoot&amp;quot; representative concentration pathway (RCP 6.0) (van Vuuren et al. 2011). pCO2 treatments were created by bubbling ambient air or a mixture of ambient air and pure CO2 that was blended continually and monitored using an infrared gas analyzer (IRGA model S151, Qubit Systems). A solenoid-controlled, gas regulation system (Model A352, Qubit Systems, Ontario, Canada) regulated the flow of CO2 and air, with pCO2 logged on a PC running LabPro software (Vemier Software and Technology). Ambient air and the elevated pCO2 mixture were supplied at ~ 10-15 L min-1 to treatment tanks using pumps (Gast pump DOA-P704-AA, see Edmunds 2011).&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;The temperatures and pCO2 levels created four treatments with two tanks treatment-1: ambient temperature-ambient pCO2 (AT-ACO2), ambient temperature-high pCO2 (AT-HCO2), high temperature-ambient pCO2 (HT-ACO2) and high temperature-high pCO2 (HT-HCO2). Treatment conditions were monitored daily, with temperature measured at 08:00, 12:00 and 18:00 hrs using a digital thermometer (Fisher Scientific model #150778, ± 0.05 °C), and light intensities at 12:00 hrs using a Li-Cor LI-193 sensor attached t 170 o a LI-1400 meter. Seawater within each tank was replaced at 200 ml/min with filtered seawater (50 micro-m) pumped from Cook’s Bay.&amp;lt;/p&amp;gt;

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&amp;lt;p&amp;gt;To evaluate the accuracy and precision of TA analyses, certified reference materials (CRM, batch 105 from A. Dickson, Scripps Institution of Oceanography) were processed before each set of seawater samples. CRMs were evaluated with a mean error of 0.37% (~ 8 micro-mol kg-1, n = 11) relative to the certified values. The precision and accuracy of pH measurements were evaluated using standardized Tris buffers (Batch 5 from A. D 193 Dickson Laboratory, Scripps Institution of Oceanography) that were processed spectrophotometrically with m-cresol as described above. Percent average error from the known pH of the Tris buffer was 0.16% (0.01 pH units, n = 13).&amp;lt;/p&amp;gt;

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