| Contributors | Affiliation | Role |
|---|---|---|
| Hassett, R. Patrick | Ohio University | Principal Investigator |
| Giner, Jose | State University of New York College of Environmental Science and Forestry (SUNY ESF) | Co-Principal Investigator |
| Copley, Nancy | Woods Hole Oceanographic Institution (WHOI BCO-DMO) | BCO-DMO Data Manager |
| Marine algal sterols used in metabolic studies with Artemia and Acartia tonsa | ||
| Id# | Sterol | Natural occurrence |
| 1 | cholesterol | common |
| 2 | 24-methylenecholesterol | common |
| 3 | 24-methylcholesterol | common |
| 4 | 24-ethylidenecholesterol | common |
| 5 | 24-ethylcholesterol | common |
| 6 | 24-propylidenecholesterol | brown tide |
| 7 | 27-norbrassicasterol | variant of brevesterol |
| 8 | brassicasterol | common |
| 9 | 23-methylbrassicasterol | diatoms |
| 10 | 24,25-methylenecholesterol | sponges and fungi |
| 11 | ∆0 4a-methylcholestanol | variant of dinosterol |
| 12 | ∆8(14)4a-methylcholestanol | variant of brevesterol |
| 13 | brevesterol | Karenia |
| 14 | gymnodynosterol | Karenia |
| 15 | gorgosterol | Alexandrium |
| 16 | 24-epibrassicasterol | common |
| 18 | 24-methylpavloval | Pavlova |
| 19 | pfiesterol | Pfiesteria |
| Marine algal sterols used in studies of effects on growth and reproduction of Artemia, Eurytemora affinis, and Acartia tonsa | ||
| Id# | Sterol | Natural occurrence |
| 2 | 24-methylenecholesterol | common |
| 3 | 24-methylcholesterol | common |
| 6 | 24-propylidenecholesterol | brown tide |
| 7 | 27-norbrassicasterol | variant of brevesterol |
| 9 | 23-methylbrassicasterol | common |
| 10 | 24,25-methylenecholesterol | sponges and fungi |
| 13 | brevesterol | Karenia |
| 15 | gorgosterol | Alexandrium |
| 20 | 24-methyl-androsterone | derived from pfiesterol |
| 21 | 24 methylpavlovol | Pavlova |
| 22 | dinosterol | dinoflagellates |
| 23 | cholesta-pavlovol | Pavlova |
| 24 | 4-methylenecholestanol | ? |
| 25 | petrosterol | sponges and fungi |
| File |
|---|
sterols_zoop.csv (Comma Separated Values (.csv), 2.58 KB) MD5:aa0a8cfd8d3e206e2ea2e55f52336f6a Primary data file for dataset ID 643054 |
| Parameter | Description | Units |
| expt_type | type of experiment using marine algal sterols | unitless |
| species | species tested | unitless |
| sterol_id | sterol identification number | unitless |
| sterol_name | chemical name | unitless |
| natural_occurrence | where sterol is found in nature | unitless |
| Dataset-specific Instrument Name | |
| Generic Instrument Name | In-situ incubator |
| Dataset-specific Description | Sanyo MIR252 incubator |
| Generic Instrument Description | A device on a ship or in the laboratory that holds water samples under controlled conditions of temperature and possibly illumination. |
| Dataset-specific Instrument Name | |
| Generic Instrument Name | Camera |
| Dataset-specific Description | Nikon CoolPix950 digital camera |
| Generic Instrument Description | All types of photographic equipment including stills, video, film and digital systems. |
| Dataset-specific Instrument Name | |
| Generic Instrument Name | Microscope - Optical |
| Dataset-specific Description | Olympus SZH30 stereo microscope |
| Generic Instrument Description | Instruments that generate enlarged images of samples using the phenomena of reflection and absorption of visible light. Includes conventional and inverted instruments. Also called a "light microscope". |
| Dataset-specific Instrument Name | |
| Generic Instrument Name | scale |
| Dataset-specific Description | Mettler AE analytical balance |
| Generic Instrument Description | An instrument used to measure weight or mass. |
| Dataset-specific Instrument Name | |
| Generic Instrument Name | Shaker |
| Dataset-specific Description | LabGenius Digital Orbital Shaker |
| Generic Instrument Description | A Shaker is a piece of lab equipment used to mix, blend, or to agitate substances in tube(s) or flask(s) by shaking them, which is mainly used in the fields of chemistry and biology. A shaker contains an oscillating board which is used to place the flasks, beakers, test tubes, etc. |
Description from NSF award abstract:
Autotroph-herbivore interactions in marine food webs are important to fisheries, the global carbon cycle, and, because of harmful algal blooms, human health. The recent hypothesis that harmful algae interfere with the growth and reproduction of zooplankton because of specific structural modifications of the algal sterols will be tested in research on the roles of nutritional factors in planktonic food webs. The effects of marine algal sterols on herbivorous crustaceans will be investigated in three calanoid copepods, Acartia hudsonica, Eurytemora affinis, and Calanus finmarchicus, and brine shrimp, Artemia salina. In this project, studies will be carried out to determine whether marine algal sterols can be metabolized to cholesterol by zooplankton and the relative efficiency of this process. This information is critical for assessing the nutritional value of different algal diets. Using the metabolic studies as a foundation, further experiments will seek to determine whether selected sterols, some of which have structural similarities to steroid hormones, have an inhibitory impact on the growth and reproduction of crustaceans. The analytical techniques used in these experiments will be high-field 13C-nuclear magnetic resonance spectrometry (NMR) and gas chromatography-high resolution mass spectrometry (GC-HRMS). Test sterols for these experiments will be labeled with stable isotopes (13C and 2H) in specific positions by chemical synthesis.
| Funding Source | Award |
|---|---|
| NSF Division of Ocean Sciences (NSF OCE) |