http://lod.bco-dmo.org/id/dataset/650175
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2016-06-28
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Metadata from sediment samples collected at Paleochori Bay, Milos Island, Greece in 2012 (Hydrothermal Autotrophic Carbon Fixation project)
2016-06-28
publication
2016-06-28
revision
BCO-DMO Linked Data URI
2016-06-28
creation
http://lod.bco-dmo.org/id/dataset/650175
Costantino Vetriani
Rutgers University
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Vetriani, C. (2016) Metadata from sediment samples collected at Paleochori Bay, Milos Island, Greece in 2012 (Hydrothermal Autotrophic Carbon Fixation project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). Version Date 2016-06-28 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/650175 [access date]
Metadata from sediment samples collected at Paleochori Bay, Milos island, Greece Dataset Description: <p>List of samples collected at the shallow-water hydrothermal vent sites at Paleochori Bay, Milos Island, Greece, and&nbsp;includes the sample description, collection&nbsp;distance from the vent, type and amount of nucleic acids.</p>
<p><strong>Related Datasets:</strong><br />
<a href="http://www.bco-dmo.org/dataset/650224">GenBank accessions</a><br />
<a href="http://www.bco-dmo.org/dataset/650210">Microbial isolates</a></p> Methods and Sampling: <p>Sequence data (bacterial ssrRNA) was obtained using the Ion Torrent platform from total RNA extracted from 32 sediment and microbial biofilm samples characterized along a transect. Sequence data are currently being generated and analyzed, they will be deposited in GenBank prior to publication and will be made available to the scientific community.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1124141 Award URL: http://www.nsf.gov/awardsearch/showAward?AWD_ID=1124141
completed
Costantino Vetriani
Rutgers University
732-932-6555 x373
Rutgers University 71 Dudley Road
New Brunswick
NJ
08901
USA
vetriani@marine.rutgers.edu
pointOfContact
asNeeded
Unknown
lat
lon
sample_description
sample_id
dist_vent_cm
sampling_instr
preservation
core_depth
date_collected
nucleic_acid
nucleic_acid_conc_ng_ul
cDNA_conc_ng_ul
theme
None, User defined
latitude
longitude
sample description
sample identification
No BCO-DMO term
instrument
depth
date
featureType
BCO-DMO Standard Parameters
Automated DNA Sequencer
Push Corer
instrument
BCO-DMO Standard Instruments
Milos_vents_2012
service
Deployment Activity
Milos Island, Greece
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Center for Dark Energy Biosphere Investigations
http://www.darkenergybiosphere.org
Center for Dark Energy Biosphere Investigations
The mission of the Center for Dark Energy Biosphere Investigations (C-DEBI) is to explore life beneath the seafloor and make transformative discoveries that advance science, benefit society, and inspire people of all ages and origins.
C-DEBI provides a framework for a large, multi-disciplinary group of scientists to pursue fundamental questions about life deep in the sub-surface environment of Earth. The fundamental science questions of C-DEBI involve exploration and discovery, uncovering the processes that constrain the sub-surface biosphere below the oceans, and implications to the Earth system. What type of life exists in this deep biosphere, how much, and how is it distributed and dispersed? What are the physical-chemical conditions that promote or limit life? What are the important oxidation-reduction processes and are they unique or important to humankind? How does this biosphere influence global energy and material cycles, particularly the carbon cycle? Finally, can we discern how such life evolved in geological settings beneath the ocean floor, and how this might relate to ideas about the origin of life on our planet?
C-DEBI's scientific goals are pursued with a combination of approaches:
(1) coordinate, integrate, support, and extend the research associated with four major programs—Juan de Fuca Ridge flank (JdF), South Pacific Gyre (SPG), North Pond (NP), and Dorado Outcrop (DO)—and other field sites;
(2) make substantial investments of resources to support field, laboratory, analytical, and modeling studies of the deep subseafloor ecosystems;
(3) facilitate and encourage synthesis and thematic understanding of submarine microbiological processes, through funding of scientific and technical activities, coordination and hosting of meetings and workshops, and support of (mostly junior) researchers and graduate students; and
(4) entrain, educate, inspire, and mentor an interdisciplinary community of researchers and educators, with an emphasis on undergraduate and graduate students and early-career scientists.
Note: Katrina Edwards was a former PI of C-DEBI; James Cowen is a former co-PI.
Data Management:
C-DEBI is committed to ensuring all the data generated are publically available and deposited in a data repository for long-term storage as stated in their Data Management Plan (PDF) and in compliance with the NSF Ocean Sciences Sample and Data Policy. The data types and products resulting from C-DEBI-supported research include a wide variety of geophysical, geological, geochemical, and biological information, in addition to education and outreach materials, technical documents, and samples. All data and information generated by C-DEBI-supported research projects are required to be made publically available either following publication of research results or within two (2) years of data generation.
To ensure preservation and dissemination of the diverse data-types generated, C-DEBI researchers are working with BCO-DMO Data Managers make data publicly available online. The partnership with BCO-DMO helps ensure that the C-DEBI data are discoverable and available for reuse. Some C-DEBI data is better served by specialized repositories (NCBI's GenBank for sequence data, for example) and, in those cases, BCO-DMO provides dataset documentation (metadata) that includes links to those external repositories.
C-DEBI
largerWorkCitation
program
Autotrophic carbon fixation at a shallow-water hydrothermal system: Constraining microbial activity, isotopic and geochemical regimes
https://www.bco-dmo.org/project/473892
Autotrophic carbon fixation at a shallow-water hydrothermal system: Constraining microbial activity, isotopic and geochemical regimes
<p>In this project we studied the shallow-water hydrothermal vent sites at Milos Island (Greece) to better understand the extent of autotrophic carbon fixation and its chemical and isotopic signature along environmental (redox/thermal) gradients. This was a 12-day long expedition (May 18 to 30, 2012) to sample vent fluids, gases and retrieve sediment cores at Paleochori Bay by using SCUBA diving at 8-10 m depth. In addition to the submarine vent sites, two subaerial locations of venting were identified at 36o 40' 28"N - 24o 31' 14" E and 36o 40' 25" N - 24o 30' 44" E. Both the subaerial and submarine sites are located on the same fracture zone that likely controls the hydrothermal circulation of evolved meteoritic water and seawater within the magmatic zone of Milos Island. To this end, the geochemistry of the fluids and gases emitted from subaerial sites provide important information towards identifying the linkage between the subaerial and submarine magmatic activity and provide insights on the metabolic functions (e.g. H2 oxidation, Fe(III) reduction, C and S cycling) of the subsurface microbial community. </p>
<p>Abstract:<br />
Currently, there is only limited information on the identity and activity of the microorganisms carrying out CO2-fixation in situ, despite the fact that these organisms form the basis of their respective ecosystems. Representatives that are able to grow autotrophically are known to exist in almost all major groups of prokaryotes, and these organisms play essential roles in ecosystems by providing a continuous supply of organic carbon for heterotrophs. Microorganisms present in extreme environments utilize CO2- fixation pathways other than the Calvin-Benson-Bassham (CBB) cycle. At present, five alternative autotrophic CO2 fixation pathways are known. Different carbon fixation pathways result in distinct isotopic signatures of the produced biomass due to the isotopic discrimination between light (12C) and heavy (13C) carbon by the carboxylating enzymes. Thus, inferences about the carbon fixation pathway predominantly utilized by the microbial community can also be made based on the stable carbon isotopic composition of the organic matter, in extant systems as well as in the geological record. However, at present little is known about the systematics and extents of fractionation during carbon fixation by prokaryotic organisms, and to our knowledge no studies exist that have systematically studied the relationship between the operation of different carbon fixation pathways and how this is reflected in the stable carbon isotopic composition in a natural system. This is a 2-year interdisciplinary, international research program that employs a powerful combination of cutting-edge research tools aiming to improve our understanding of autotrophic carbon fixation and its chemical and isotopic signature along environmental gradients in a natural hydrothermal system. The following hypotheses are addressed:</p>
<p>1. The diversity of microorganisms present along a thermal and redox gradient, and rates of CO2 fixation, will reflect adaptation to in situ temperatures and geochemical conditions</p>
<p>2. Microorganisms utilizing the CBB cycle for autotrophic CO2-fixation will represent a smaller percentage of the chemolithoautotrophic community at higher temperatures, where microorganisms utilizing alternative CO2-fixation pathways dominate</p>
<p>3. Isotopic values of biomass and specific biomarker molecules will vary along a thermal and redox gradient from zones characterized by a higher hydrothermal fluid flux and thus higher temperatures to the surrounding, cooler areas, corresponding to the physiology of the microorganisms utilizing different pathways for carbon fixation</p>
<p>The PIs will use a multidisciplinary approach to delineate the relative contribution of the different carbon fixation pathways along an environmental gradient by combining metagenomic analyses coupled with: 1) an assessment of the frequency and the expression of specific key genes involved in carbon fixation, and 2) with the measurement of carbon fixation rates. These data will be integrated with the determination of stable C isotopic composition of biomass, DIC, and specific hydrocarbons/lipids. Due to its easy accessibility, well-established environmental gradients, and extensive background information, the shallow-water vents off Milos (Greece) will be used as a natural laboratory to perform these studies.</p>
<p>Intellectual Merit. The data generated in this study will allow constraints on the relationship between autotrophic carbon fixation and the resulting isotopic signatures of biomass and specific biomarker molecules (e.g. CH4, C2+ alkanes, lipids) in a natural system. This has implications for assessing the importance of carbon fixation in extant ecosystems, and it will also provide a tool to improve the interpretation of isotopic values in the geological record.</p>
Hydrothermal Autotrophic Carbon Fixation
largerWorkCitation
project
eng; USA
biota
oceans
Milos Island, Greece
2016-06-28
Shallow-water hydrothermal vents, Paleochori Bay, Milos Island, Greece
0
BCO-DMO catalogue of parameters from Metadata from sediment samples collected at Paleochori Bay, Milos Island, Greece in 2012 (Hydrothermal Autotrophic Carbon Fixation project)
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/650197.rdf
Name: lat
Units: decimal degrees
Description: latitude; north is positive
http://lod.bco-dmo.org/id/dataset-parameter/650198.rdf
Name: lon
Units: decimal degrees
Description: longitude; east is positive
http://lod.bco-dmo.org/id/dataset-parameter/650199.rdf
Name: sample_description
Units: unitless
Description: type of sample: from sediment of microbial mat
http://lod.bco-dmo.org/id/dataset-parameter/650200.rdf
Name: sample_id
Units: unitless
Description: sample identification
http://lod.bco-dmo.org/id/dataset-parameter/650201.rdf
Name: dist_vent_cm
Units: cm
Description: distance from vent center
http://lod.bco-dmo.org/id/dataset-parameter/650202.rdf
Name: sampling_instr
Units: unitless
Description: instrument used to collect sample
http://lod.bco-dmo.org/id/dataset-parameter/650203.rdf
Name: preservation
Units: unitless
Description: sample preservative
http://lod.bco-dmo.org/id/dataset-parameter/650204.rdf
Name: core_depth
Units: cm
Description: depth within the sediment core
http://lod.bco-dmo.org/id/dataset-parameter/650205.rdf
Name: date_collected
Units: unitless
Description: date sampled
http://lod.bco-dmo.org/id/dataset-parameter/650206.rdf
Name: nucleic_acid
Units: unitless
Description: nucleic acid extracted
http://lod.bco-dmo.org/id/dataset-parameter/650207.rdf
Name: nucleic_acid_conc_ng_ul
Units: nanograms/microliter
Description: nucleic acid concentration
http://lod.bco-dmo.org/id/dataset-parameter/650208.rdf
Name: cDNA_conc_ng_ul
Units: nanograms/microliter
Description: cDNA concentration
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
3851
https://datadocs.bco-dmo.org/file/JEE1nGjCK603gr/sample_log.csv
sample_log.csv
Primary data file for dataset ID 650175
download
https://www.bco-dmo.org/dataset/650175/data/download
download
onLine
dataset
<p>Sequence data (bacterial ssrRNA) was obtained using the Ion Torrent platform from total RNA extracted from 32 sediment and microbial biofilm samples characterized along a transect. Sequence data are currently being generated and analyzed, they will be deposited in GenBank prior to publication and will be made available to the scientific community.</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
PI Supplied Instrument Name: PI Supplied Instrument Description:Ion Torrent platform Instrument Name: Automated DNA Sequencer Instrument Short Name:Automated Sequencer Instrument Description: General term for a laboratory instrument used for deciphering the order of bases in a strand of DNA. Sanger sequencers detect fluorescence from different dyes that are used to identify the A, C, G, and T extension reactions. Contemporary or Pyrosequencer methods are based on detecting the activity of DNA polymerase (a DNA synthesizing enzyme) with another chemoluminescent enzyme. Essentially, the method allows sequencing of a single strand of DNA by synthesizing the complementary strand along it, one base pair at a time, and detecting which base was actually added at each step.
PI Supplied Instrument Name: PI Supplied Instrument Description:push cores were collected by SCUBA divers Instrument Name: Push Corer Instrument Short Name: Instrument Description: Capable of being performed in numerous environments, push coring is just as it sounds. Push coring is simply pushing the core barrel (often an aluminum or polycarbonate tube) into the sediment by hand. A push core is useful in that it causes very little disturbance to the more delicate upper layers of a sub-aqueous sediment.
Description obtained from: http://web.whoi.edu/coastal-group/about/how-we-work/field-methods/coring/
Deployment: Milos_vents_2012
Milos_vents_2012
shoreside Milos_vents
island
Milos_vents_2012
Dionysis Foustoukos
Carnegie Institution for Science
shoreside Milos_vents
island