http://lod.bco-dmo.org/id/dataset/651520
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2016-07-08
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Laboratory experiment analyzing the photosynthetic and calcification rates of Pleurochrysis carterae (Ocean acidification effects on copes and coccoliths project)
2016-07-11
publication
2016-07-11
revision
BCO-DMO Linked Data URI
2016-07-11
creation
http://lod.bco-dmo.org/id/dataset/651520
William M. Balch
Bigelow Laboratory for Ocean Sciences
principalInvestigator
David Fields
Bigelow Laboratory for Ocean Sciences
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Balch, W. M., Fields, D. (2016) Laboratory experiment analyzing the photosynthetic and calcification rates of Pleurochrysis carterae (Ocean acidification effects on copes and coccoliths project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version Final) Version Date 2016-07-11 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/651520 [access date]
Photosynthetic and calcification rates of Pleurochrysis carterae Dataset Description: <p>Photosynthetic and calcification rates of <em>Pleurochrysis carterae </em>at three&nbsp;pCO2 levels.</p> Methods and Sampling: <p><strong>Cultures:</strong> <em>Pleurochrysis carterae </em>cultures were maintained in exponential growth phase under axenic conditions in semi-continuous batch culture using L1-Si media prepared on 0.2 um-filtered, UV-sterilized, autoclaved seawater.&nbsp; Cultures were acclimated to one of three <em>p</em>CO2&nbsp;treatments for over&nbsp;9 generations before experiments were performed.</p>
<p><strong><em>p</em>CO2</strong>: Carbonate chemistry was manipulated by bubbling cultures and prepared media with 500 mL/min^-1&nbsp;with 0.2 um-filtered 280, 380, or 750 ppm <em>p</em>CO2&nbsp;air.&nbsp; The <em>p</em>CO2&nbsp;levels of the treatment air were established using two mass flow controllers (Aalborg, Orangeburg, NY, USA) for each treatment to precisely mix in-house compressed air and pure CO2&nbsp;(Maine Oxy, Auburn, ME, USA).&nbsp; The in-house compressed air was stripped of CO2&nbsp;to less than 10 ppm CO2&nbsp;using a Puregas VCD CO2&nbsp;Adsorber (Puregas, LLC, Broomfield, CO, USA).&nbsp; The <em>p</em>CO2&nbsp;of the gas mixtures was stable to 8 ppm.</p>
<p><strong>Length of Incubation (hours)</strong>: Number of hours that algae were incubated with 14C-bicarbonate.</p>
<p><strong>Cell density (cells/</strong><strong>mL-1)</strong>: Culture density measured using a Moxi Z mini automated cell counter (ORFLO Technologies, Ketchum, ID, USA), which has a coefficient of variation of 4%.</p>
<p><strong>Photosynthetic rate (u</strong><strong>g C</strong>/<strong>L^-1&nbsp;d^-1) and Calcification rate (u</strong><strong>g C/</strong><strong>L^-1&nbsp;d^-1):&nbsp; </strong>Triplicate samples from each <em>p</em>CO2&nbsp;treatment were spiked with 14C-HCO3-&nbsp;and incubated at 16.5 +/-&nbsp;0.5 degrees Celsius&nbsp;and 415 umol photons/m^-2/s^-1&nbsp;PAR for 3 h.&nbsp; At the end of the incubation, the cells from each replicate, along with triplicate formalin-killed blanks for each <em>p</em>CO2&nbsp;treatment, were filtered onto 0.4 um polycarbonate filters and their carbon was partitioned into organic and inorganic fractions by acidification and subsequent capture of 14CO2&nbsp;(from PIC) in a trap containing a Whatman GFA filter presoaked with 0.2 mL phenethylamine (see Balch et al. 2000 for detailed methodology).&nbsp; The radioactivity of each fraction was measured on a Packard Tri-Carb 2750 LL scintillation counter (acquired by Perkin Elmer, Waltham, MA, USA) and the photosynthetic rate and calcification rate were calculated from the organic carbon and inorganic carbon fractions, respectively, using the equation on p. 118 from Parsons et al. (1984) and applying appropriate unit conversions:</p>
<p>Photosynthesis OR Calcification (mg C • (m^3)^-1&nbsp;d^-1)<sub> =&nbsp; </sub>[(<em>Rs&nbsp;– Rb</em>)<em> * W * </em>1.05]/(<em>R * t</em>)</p>
<p>Where <em>R</em> is the total activity (dpm) of bicarbonate added; <em>t</em>&nbsp; is the length of incubation (in days); <em>Rs</em>&nbsp;is the sample count (dpm), <em>Rb</em>&nbsp;is the formalin blank count (dpm), and <em>W</em> is the weight of total carbon dioxide present in mg C • (m^3)^-1, determined from the expression <em>W</em>=12,000*<em>TC</em>, where <em>TC</em> is the total carbon dioxide, which can be approximated from salinity, as described in Parsons et al. (1984). The factor of 1.05 accounts for preferential uptake of 12C.&nbsp; The calculation is the same for photosynthetic rate and calcification rate – the difference is using the sample count from either the organic fraction or the inorganic fraction, respectively.&nbsp;</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1220068 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1220068
completed
William M. Balch
Bigelow Laboratory for Ocean Sciences
207-315-2567
60 Bigelow Drive
East Boothbay
ME
04544
USA
bbalch@bigelow.org
pointOfContact
David Fields
Bigelow Laboratory for Ocean Sciences
207-315-2567
60 Bigelow Dr.
East Boothbay
ME
04544
USA
dfields@bigelow.org
pointOfContact
asNeeded
Dataset Version: Final
Unknown
replicate
CO2_treatment
cell_density
photo_rate
calcification_rate
photoRate_LDcycle
calcRate_LDcycle
photoRate_cellDensity_ugC
photoRate_cellDensity_pmolC
calcRate_cellDensity_ugC
calcRate_cellDensity_pmolC
POC_ratio
photoRate_mean
photoRate_SD
calcRate_mean
calcRate_SD
POC_ratio_mean
POC_ratio_SD
Moxi Z Mini Automated Cell Counter
Puregas VCD CO2 Adsorber
Packard Tri-Carb 2750 LL
Aalborg Mass Flow Controller
theme
None, User defined
replicate
treatment
cell_concentration
photosynthetic rate
calcification
No BCO-DMO term
mean
standard deviation
featureType
BCO-DMO Standard Parameters
Automated Cell Counter
CO2 Adsorber
Liquid Scintillation Counter
Mass Flow Controller
instrument
BCO-DMO Standard Instruments
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Science, Engineering and Education for Sustainability NSF-Wide Investment (SEES): Ocean Acidification (formerly CRI-OA)
https://www.nsf.gov/funding/pgm_summ.jsp?pims_id=503477
Science, Engineering and Education for Sustainability NSF-Wide Investment (SEES): Ocean Acidification (formerly CRI-OA)
NSF Climate Research Investment (CRI) activities that were initiated in 2010 are now included under Science, Engineering and Education for Sustainability NSF-Wide Investment (SEES). SEES is a portfolio of activities that highlights NSF's unique role in helping society address the challenge(s) of achieving sustainability. Detailed information about the SEES program is available from NSF (https://www.nsf.gov/funding/pgm_summ.jsp?pims_id=504707).
In recognition of the need for basic research concerning the nature, extent and impact of ocean acidification on oceanic environments in the past, present and future, the goal of the SEES: OA program is to understand (a) the chemistry and physical chemistry of ocean acidification; (b) how ocean acidification interacts with processes at the organismal level; and (c) how the earth system history informs our understanding of the effects of ocean acidification on the present day and future ocean.
Solicitations issued under this program:
NSF 10-530, FY 2010-FY2011
NSF 12-500, FY 2012
NSF 12-600, FY 2013
NSF 13-586, FY 2014
NSF 13-586 was the final solicitation that will be released for this program.
PI Meetings:
1st U.S. Ocean Acidification PI Meeting(March 22-24, 2011, Woods Hole, MA)
2nd U.S. Ocean Acidification PI Meeting(Sept. 18-20, 2013, Washington, DC)
3rd U.S. Ocean Acidification PI Meeting (June 9-11, 2015, Woods Hole, MA – Tentative)
NSF media releases for the Ocean Acidification Program:
Press Release 10-186 NSF Awards Grants to Study Effects of Ocean Acidification
Discovery Blue Mussels "Hang On" Along Rocky Shores: For How Long?
Discovery nsf.gov - National Science Foundation (NSF) Discoveries - Trouble in Paradise: Ocean Acidification This Way Comes - US National Science Foundation (NSF)
Press Release 12-179 nsf.gov - National Science Foundation (NSF) News - Ocean Acidification: Finding New Answers Through National Science Foundation Research Grants - US National Science Foundation (NSF)
Press Release 13-102 World Oceans Month Brings Mixed News for Oysters
Press Release 13-108 nsf.gov - National Science Foundation (NSF) News - Natural Underwater Springs Show How Coral Reefs Respond to Ocean Acidification - US National Science Foundation (NSF)
Press Release 13-148 Ocean acidification: Making new discoveries through National Science Foundation research grants
Press Release 13-148 - Video nsf.gov - News - Video - NSF Ocean Sciences Division Director David Conover answers questions about ocean acidification. - US National Science Foundation (NSF)
Press Release 14-010 nsf.gov - National Science Foundation (NSF) News - Palau's coral reefs surprisingly resistant to ocean acidification - US National Science Foundation (NSF)
Press Release 14-116 nsf.gov - National Science Foundation (NSF) News - Ocean Acidification: NSF awards $11.4 million in new grants to study effects on marine ecosystems - US National Science Foundation (NSF)
SEES-OA
largerWorkCitation
program
Effects of ocean acidification on Emiliania huxleyi and Calanus finmarchicus; insights into the oceanic alkalinity and biological carbon pumps
https://osprey.bco-dmo.org/project/514415
Effects of ocean acidification on Emiliania huxleyi and Calanus finmarchicus; insights into the oceanic alkalinity and biological carbon pumps
<p><em>(Extracted from the NSF award abstract)</em></p>
<p>Ocean acidification is one of the most pressing marine science issues of our time, with potential biological impacts spanning all marine phyla and potential societal impacts affecting man's relationship to the sea. Rising levels of atmospheric pCO2 are increasing the acidity of the world oceans. It is generally held that average surface ocean pH has already declined by 0.1 pH units relative to the pre-industrial level (Orr et al., 2005), and is projected to decrease 0.3 to 0.46 units by the end of this century, depending on CO2 emission scenarios (Caldeira and Wickett, 2005). The overall goal of this research is to parameterize how changes in pCO2 levels could alter the biological and alkalinity pumps of the world ocean. Specifically, the direct and indirect effects of ocean acidification will be examined within a simple, controlled predator/prey system containing a single prey phytoplankton species (the coccolithophore, Emiliania huxleyi) and a single predator (the oceanic metazoan grazer, Calanus finmarchicus). The experiments are designed to elucidate both direct effects (i.e. effects of ocean acidification on the individual organisms only) and interactive effects (i.e. effects on the combined predator/prey system). Interactive experiments with phytoplankton prey and zooplankton predator are a critical starting point for predicting the overall impact of ocean acidification in marine ecosystems. To meet these goals, a state-of-the-art facility will be constructed with growth chambers that are calibrated and have highly-controlled pH and alkalinity levels. The strength of this approach lies in meticulous calibration and redundant measurements that will be made to ensure that conditions within the chambers are well described and tightly monitored for DIC levels. Growth and calcification rates in coccolithophores and the developmental rates, morphological and behavioral effects on copepods will be measured. The PIC and POC in the algae and the excreted fecal pellets will be monitored for changes in the PIC/POC ratio, a key parameter for modeling feedback mechanisms for rising pCO2 levels. In addition, 14C experiments are planned to measure calcification rates in coccolithophores and dissolution rates as a result of grazing. These key experiments will verify closure in the mass balance of PIC, allowing the determination of actual dissolution rates of PIC within the guts of copepod grazers.</p>
OA_Copes_Coccoliths
largerWorkCitation
project
eng; USA
oceans
2016-07-11
Laboratory experiments; East Boothbay, Maine
0
BCO-DMO catalogue of parameters from Laboratory experiment analyzing the photosynthetic and calcification rates of Pleurochrysis carterae (Ocean acidification effects on copes and coccoliths project)
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/651538.rdf
Name: replicate
Units: unitless
Description: <p>Denotes the three replicates for each pCO2 treatment.</p>
http://lod.bco-dmo.org/id/dataset-parameter/651539.rdf
Name: CO2_treatment
Units: parts per million
Description: <p>The independent variable; one of three pCO2 levels: 280 ppm; 380 ppm; 750 ppm</p>
http://lod.bco-dmo.org/id/dataset-parameter/651540.rdf
Name: cell_density
Units: cells per milliliter
Description: <p>Density of cells in each replicate.</p>
http://lod.bco-dmo.org/id/dataset-parameter/651541.rdf
Name: photo_rate
Units: micrograms per liter per day
Description: <p>Rate of organic carbon production by Pleurochrysis based on a 3 h incubation in the light; divided by 0.125 (3 h/24 h) to give a rate per day.</p>
http://lod.bco-dmo.org/id/dataset-parameter/651542.rdf
Name: calcification_rate
Units: micrograms per liter per day
Description: <p>Rate of inorganic carbon production by Pleurochrysis based on a 3 h incubation in the light, but divided by 0.125 (3 h/24 h) to give a rate per day.</p>
http://lod.bco-dmo.org/id/dataset-parameter/651543.rdf
Name: photoRate_LDcycle
Units: micrograms per liter per day
Description: <p>Rate of organic carbon production by Pleurochrysis corrected to a 14-10 h light-dark cycle.</p>
http://lod.bco-dmo.org/id/dataset-parameter/651544.rdf
Name: calcRate_LDcycle
Units: micrograms per liter per day
Description: <p>Rate of inorganic carbon production by Pleurochrysis corrected to a 14-10 h light-dark cycle taking into consideration calcification in the dark.</p>
http://lod.bco-dmo.org/id/dataset-parameter/651545.rdf
Name: photoRate_cellDensity_ugC
Units: micrograms per cell per day
Description: <p>P24 corrected to the cell density of the replicate to give a per cell photosynthetic rate.</p>
http://lod.bco-dmo.org/id/dataset-parameter/651546.rdf
Name: photoRate_cellDensity_pmolC
Units: picomoles per cell per day
Description: <p>Unit conversion to give photosynthetic rate in pmol units.</p>
http://lod.bco-dmo.org/id/dataset-parameter/651547.rdf
Name: calcRate_cellDensity_ugC
Units: micrograms per cell per day
Description: <p>C24 corrected to the cell density of the replicate to give a per cell calcification rate.</p>
http://lod.bco-dmo.org/id/dataset-parameter/651548.rdf
Name: calcRate_cellDensity_pmolC
Units: picomoles per cell per day
Description: <p>Unit conversion to give calcification rate in pmol units.</p>
http://lod.bco-dmo.org/id/dataset-parameter/651549.rdf
Name: POC_ratio
Units: dimensionless
Description: <p>Calculation of the particulate inorganic (PIC) to particulate organic (POC) ratio of P. carterae.</p>
http://lod.bco-dmo.org/id/dataset-parameter/651550.rdf
Name: photoRate_mean
Units: picomoles per cell per day
Description: <p>Mean of the photosynthetic rate. Three replicates per mean.</p>
http://lod.bco-dmo.org/id/dataset-parameter/651551.rdf
Name: photoRate_SD
Units: picomoles per cell per day
Description: <p>Standard deviation of the photosynthetic rate. Three replicates per standard deviation.</p>
http://lod.bco-dmo.org/id/dataset-parameter/651552.rdf
Name: calcRate_mean
Units: picomoles per cell per day
Description: <p>Mean of the calcification rate. Three replicates per mean.</p>
http://lod.bco-dmo.org/id/dataset-parameter/651553.rdf
Name: calcRate_SD
Units: picomoles per cell per day
Description: <p>Standard deviation of the calcification rate. Three replicates per standard deviation.</p>
http://lod.bco-dmo.org/id/dataset-parameter/651554.rdf
Name: POC_ratio_mean
Units: dimensionless
Description: <p>Mean of PIC to POC ratio. Three replicates per mean.</p>
http://lod.bco-dmo.org/id/dataset-parameter/651555.rdf
Name: POC_ratio_SD
Units: dimensionless
Description: <p>Standard deviation of the PIC to POC ratio. Three replicates per standard deviation.</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
1215
https://datadocs.bco-dmo.org/file/EKKygrBh3lWlQz/photo_calc_rates.csv
photo_calc_rates.csv
Primary data file for dataset ID 651520
download
https://osprey.bco-dmo.org/dataset/651520/data/download
download
onLine
dataset
<p><strong>Cultures:</strong> <em>Pleurochrysis carterae </em>cultures were maintained in exponential growth phase under axenic conditions in semi-continuous batch culture using L1-Si media prepared on 0.2 um-filtered, UV-sterilized, autoclaved seawater.&nbsp; Cultures were acclimated to one of three <em>p</em>CO2&nbsp;treatments for over&nbsp;9 generations before experiments were performed.</p>
<p><strong><em>p</em>CO2</strong>: Carbonate chemistry was manipulated by bubbling cultures and prepared media with 500 mL/min^-1&nbsp;with 0.2 um-filtered 280, 380, or 750 ppm <em>p</em>CO2&nbsp;air.&nbsp; The <em>p</em>CO2&nbsp;levels of the treatment air were established using two mass flow controllers (Aalborg, Orangeburg, NY, USA) for each treatment to precisely mix in-house compressed air and pure CO2&nbsp;(Maine Oxy, Auburn, ME, USA).&nbsp; The in-house compressed air was stripped of CO2&nbsp;to less than 10 ppm CO2&nbsp;using a Puregas VCD CO2&nbsp;Adsorber (Puregas, LLC, Broomfield, CO, USA).&nbsp; The <em>p</em>CO2&nbsp;of the gas mixtures was stable to 8 ppm.</p>
<p><strong>Length of Incubation (hours)</strong>: Number of hours that algae were incubated with 14C-bicarbonate.</p>
<p><strong>Cell density (cells/</strong><strong>mL-1)</strong>: Culture density measured using a Moxi Z mini automated cell counter (ORFLO Technologies, Ketchum, ID, USA), which has a coefficient of variation of 4%.</p>
<p><strong>Photosynthetic rate (u</strong><strong>g C</strong>/<strong>L^-1&nbsp;d^-1) and Calcification rate (u</strong><strong>g C/</strong><strong>L^-1&nbsp;d^-1):&nbsp; </strong>Triplicate samples from each <em>p</em>CO2&nbsp;treatment were spiked with 14C-HCO3-&nbsp;and incubated at 16.5 +/-&nbsp;0.5 degrees Celsius&nbsp;and 415 umol photons/m^-2/s^-1&nbsp;PAR for 3 h.&nbsp; At the end of the incubation, the cells from each replicate, along with triplicate formalin-killed blanks for each <em>p</em>CO2&nbsp;treatment, were filtered onto 0.4 um polycarbonate filters and their carbon was partitioned into organic and inorganic fractions by acidification and subsequent capture of 14CO2&nbsp;(from PIC) in a trap containing a Whatman GFA filter presoaked with 0.2 mL phenethylamine (see Balch et al. 2000 for detailed methodology).&nbsp; The radioactivity of each fraction was measured on a Packard Tri-Carb 2750 LL scintillation counter (acquired by Perkin Elmer, Waltham, MA, USA) and the photosynthetic rate and calcification rate were calculated from the organic carbon and inorganic carbon fractions, respectively, using the equation on p. 118 from Parsons et al. (1984) and applying appropriate unit conversions:</p>
<p>Photosynthesis OR Calcification (mg C • (m^3)^-1&nbsp;d^-1)<sub> =&nbsp; </sub>[(<em>Rs&nbsp;– Rb</em>)<em> * W * </em>1.05]/(<em>R * t</em>)</p>
<p>Where <em>R</em> is the total activity (dpm) of bicarbonate added; <em>t</em>&nbsp; is the length of incubation (in days); <em>Rs</em>&nbsp;is the sample count (dpm), <em>Rb</em>&nbsp;is the formalin blank count (dpm), and <em>W</em> is the weight of total carbon dioxide present in mg C • (m^3)^-1, determined from the expression <em>W</em>=12,000*<em>TC</em>, where <em>TC</em> is the total carbon dioxide, which can be approximated from salinity, as described in Parsons et al. (1984). The factor of 1.05 accounts for preferential uptake of 12C.&nbsp; The calculation is the same for photosynthetic rate and calcification rate – the difference is using the sample count from either the organic fraction or the inorganic fraction, respectively.&nbsp;</p>
Specified by the Principal Investigator(s)
<p><strong>Photosynthetic rate corrected to 14:10 L:D cycle (u</strong><strong>g C </strong>/&nbsp;<strong>L^-1&nbsp;d^-1):</strong>&nbsp; Because our cultures are maintained in an incubator with a 14-10 h light-dark cycle, and our photosynthetic rate (<em>P3</em>)<em> </em>was extrapolated to a per day rate based on a 3 h light incubation, we corrected for our 14 h day to obtain a true 24 h day photosynthetic rate (<em>P24</em>) as follows:</p>
<p><img src="https://datadocs.bco-dmo.org/d3/data_docs/OA_Copes_Coccoliths/equation1.png" /></p>
<p><strong>Calcification rate corrected to 14:10 L:D cycle (u</strong><strong>g C </strong>/<strong>&nbsp;L^-1&nbsp;d^-1): </strong>Because our cultures are maintained in an incubator with a 14-10 h light-dark cycle, and our calcification rate (<em>C3</em>)<em> </em>was extrapolated to a per day rate based on a 3 h light incubation, we corrected for our 14 h day to obtain a true 24 h day calcification rate (<em>C24</em>).&nbsp; In a separate experiment we determined that calcification in <em>P. carterae</em> is somewhat, but not entirely, light dependent, so we calculated <em>x</em>, the proportion of light calcification that is representative of dark calcification for each pCO2&nbsp;treatment as follows:</p>
<p><em>x(280)</em>&nbsp;= 0.0092</p>
<p><em>x(380)</em>&nbsp;= 0.1983</p>
<p><em>x(750)</em>&nbsp;= 0.2191</p>
<p>We then corrected <em>C3</em>&nbsp;to <em>C24&nbsp;</em>as follows:</p>
<p><img alt="" src="https://datadocs.bco-dmo.org/d3/data_docs/OA_Copes_Coccoliths/equation2.png" style="height:47px; width:278px" /></p>
<p><strong>Photosynthetic and calcification rates corrected to cell density (ug C /&nbsp;cell^-1&nbsp;/&nbsp;d^-1):</strong>&nbsp; To obtain <em>P24&nbsp;</em>and <em>C24</em>&nbsp;rates on a per cell basis, rates were divided by cell density and then by a volume conversion factor.</p>
<p><strong>Photosynthetic and calcification rates corrected to cell density (pmol C /&nbsp;cell^-1&nbsp;/&nbsp;d^-1): </strong>Standard unit conversion factors were applied to get photosynthetic and calcification rates in terms of pmol organic C or pmol CaCO3&nbsp;cell^-1&nbsp;day^-1, respectively.</p>
<p><strong>Calcification/Photosynthesis:</strong> To obtain a particulate inorganic carbon to particulate organic carbon ratio (PIC /&nbsp;POC^-1), the calcification rate was divided by the photosynthetic rate.</p>
<p><strong>DMO notes:</strong><br />
- added underscores and removed spaces and units from column names<br />
- changed column names to comply with BCO-DMO standards.<br />
- added "na" to blank cells in columns where SD and mean were calculated</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Moxi Z Mini Automated Cell Counter
Moxi Z Mini Automated Cell Counter
PI Supplied Instrument Name: Moxi Z Mini Automated Cell Counter PI Supplied Instrument Description:measures culture density Instrument Name: Automated Cell Counter Instrument Short Name:ACC Instrument Description: An instrument that determines the numbers, types or viability of cells present in a sample.
Puregas VCD CO2 Adsorber
Puregas VCD CO2 Adsorber
PI Supplied Instrument Name: Puregas VCD CO2 Adsorber PI Supplied Instrument Description:instrument stripped compressed air of CO2 Instrument Name: CO2 Adsorber Instrument Short Name:CO2 Adsorber Instrument Description: CO2 Adsorber - an instrument designed to remove CO2 and moisture from compressed air.
Packard Tri-Carb 2750 LL
Packard Tri-Carb 2750 LL
PI Supplied Instrument Name: Packard Tri-Carb 2750 LL PI Supplied Instrument Description:measures radioactivity Instrument Name: Liquid Scintillation Counter Instrument Short Name:LSC Instrument Description: Liquid scintillation counting is an analytical technique which is defined by the incorporation of the radiolabeled analyte into uniform distribution with a liquid chemical medium capable of converting the kinetic energy of nuclear emissions into light energy. Although the liquid scintillation counter is a sophisticated laboratory counting system used the quantify the activity of particulate emitting (ß and a) radioactive samples, it can also detect the auger electrons emitted from 51Cr and 125I samples.
Liquid scintillation counters are instruments assaying alpha and beta radiation by quantitative detection of visible light produced by the passage of rays or particles through a suitable scintillant incorporated into the sample. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB21/
Aalborg Mass Flow Controller
Aalborg Mass Flow Controller
PI Supplied Instrument Name: Aalborg Mass Flow Controller PI Supplied Instrument Description:Indicate and control set flow rates of gases. Manufactured in Orangeburg, NY USA. Instrument Name: Mass Flow Controller Instrument Short Name:MFC Instrument Description: Mass Flow Controller (MFC) - A device used to measure and control the flow of fluids and gases