http://lod.bco-dmo.org/id/dataset/651784
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2016-07-14
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Chlorophyll-a concentrations from CTD cast deployments and underway seawater inflow from Endeavor 532 and Endeavor 538 cruises in 2013 and 2014
2017-07-17
publication
2017-07-17
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2022-05-04
publication
https://doi.org/10.26008/1912/bco-dmo.651784.2
Bess B. Ward
Princeton University
principalInvestigator
Andrew E. Allen
J. Craig Venter Institute
principalInvestigator
Daniel M. Sigman
Princeton University
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Ward, B. B., Allen, A. E., Sigman, D. M. (2022) Chlorophyll-a concentrations from CTD cast deployments and underway seawater inflow from Endeavor 532 and Endeavor 538 cruises in 2013 and 2014. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 2) Version Date 2017-07-17 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.651784.2 [access date]
Chlorophyll-a concentrations from CTD cast deployments and underway seawater inflow from Endeavor 532 and Endeavor 538 Dataset Description: <p>Chlorophyll-a concentrations from&nbsp;CTD casts made during the August-September 2013 EN532 and April-May 2014 EN358 cruises aboard R/V Endeavor. Study sites are located in the subarctic Atlantic Ocean along the 20°W meridian between 50°N and 60°N.&nbsp;Two transects from the US East coast to the subarctic study sites were performed as well.</p> Methods and Sampling: <p>Discrete chlorophyll <em>a </em>(Chla) samples were measured fluorometrically (Holm-Hansen &amp; Riemann 1978) by Nicolas Van Oostende and Jessica Lueders Dumont at 6 depths spanning the surface, the bottom of the euphotic zone,&nbsp;the mixed layer, and Chla maximum depths. Up to 4 liters of seawater, retrieved directly from the Niskin bottles or the surface seawater inflow, were sequentially filtered through a 20 µm pore-size polycarbonate filter, a 2 µm pore-size polycarbonate filter, and a 0.3 µm pore-size glass fiber filter (GF-75; Sterlitech; 47 mm diameter). Filtrations were performed in the dark under low vacuum (&lt;200 mbar). The Chla filters were packaged into aluminium foil (GF filter), or a 5 ml cryovial, and immediately frozen at -80°C until analysis.</p>
<p>Chlorophyll was extracted in 90% acetone at 4°C overnight and measured using a Turner Trilogy fluorometer, calibrated against a pure Chla standard (<em>Anacystis nidulans</em> Chla, Sigma-Aldrich, Saint-Louis, USA). Measurements were corrected for the fluorescence of phaeopigments after acidification with HCl (24 mM final concentration). Limit of quantification was 1 µg per liter in extract, or approximately 1 ng per liter in the sample. The Chlorophyll <em>a</em> concentration of casts 15 to 41 was measured by James L. Pinckney using the HPLC method derived from the Van Heukelem et al. (1992, 1994) and Pinckney et al. (1996) protocols.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1136345 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1136345
completed
Bess B. Ward
Princeton University
609-258-5150
Geosciences Department 217 Guyot Hall
Princeton
NJ
08544-1003
USA
bbw@princeton.edu
pointOfContact
Andrew E. Allen
J. Craig Venter Institute
858-200-1826
4120 Capricorn Lane
La Jolla
CA
92037
USA
aallen@jcvi.org
pointOfContact
Daniel M. Sigman
Princeton University
609-258-2194
Department of Geosciences M52 Guyot Hall
Princeton
NJ
08544
USA
sigman@princeton.edu
pointOfContact
asNeeded
Dataset Version: 2
Unknown
cruise_id
cast
ISO_DateTime_UTC
lon
lat
depth_w
depth
Chla_2_20um
Chla_2_20um_flag
Chla_gt_20um
Chla_gt_20um_flag
Chla_lt_2um
Chla_lt_2um_flag
Chla_total
Chla_total_flag
theme
None, User defined
Cruise identfier
cast
ISO_DateTime_UTC
longitude
latitude
depth_w
depth
chlorophyll a
quality flag
featureType
BCO-DMO Standard Parameters
CTD Sea-Bird SBE 911plus
Fluorometer
High-Performance Liquid Chromatograph
Niskin bottle
instrument
BCO-DMO Standard Instruments
EN532
EN538
service
Deployment Activity
subarctic Atlantic Ocean
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Dimensions of Biodiversity
http://www.nsf.gov/funding/pgm_summ.jsp?pims_id=503446
Dimensions of Biodiversity
(adapted from the NSF Synopsis of Program)
Dimensions of Biodiversity is a program solicitation from the NSF Directorate for Biological Sciences. FY 2010 was year one of the program. [MORE from NSF]
The NSF Dimensions of Biodiversity program seeks to characterize biodiversity on Earth by using integrative, innovative approaches to fill rapidly the most substantial gaps in our understanding. The program will take a broad view of biodiversity, and in its initial phase will focus on the integration of genetic, taxonomic, and functional dimensions of biodiversity. Project investigators are encouraged to integrate these three dimensions to understand the interactions and feedbacks among them. While this focus complements several core NSF programs, it differs by requiring that multiple dimensions of biodiversity be addressed simultaneously, to understand the roles of biodiversity in critical ecological and evolutionary processes.
Dimensions of Biodiversity
largerWorkCitation
program
Functional diversity of marine eukaryotic phytoplankton and their contributions to the C and N cycling
https://osprey.bco-dmo.org/project/544343
Functional diversity of marine eukaryotic phytoplankton and their contributions to the C and N cycling
<p>This project will investigate the taxonomic, genetic and functional diversity of eukaryotic phytoplankton at two North Atlantic sites (subarctic and subtropical) in two seasons. The PIs will use diagnostic microarrays for community analysis based on functional genes (both DNA and RNA) and next generation sequencing (i.e., transcriptomics using 454 technology) to identify the players, both in terms of community composition and activity, and to explore the functional diversity of the natural assemblage. In order to identify which groups are active in C and N assimilation and which N source is being utilized by the different size and functional groups, both filter-separated and flow cytometry-sorted samples will be used to 1) measure 13C primary production and 15N assimilation by incubations with isotope tracers, 2) measure the natural stable N isotope signatures of different taxonomic groups and 3) link the molecular diversity to the functional diversity in C and N transformations. Using flow cytometry linked to mass spectrometry, these investigators have found an unexpectedly strong differentiation in the form of N assimilated by prokaryotes and eukaryotes, with eukaryotes being more dynamic.</p>
<p>This project will investigate the taxonomic, genetic and functional diversity of eukaryotic phytoplankton and to link this diversity and assemblage composition to the carbon and nitrogen biogeochemistry of the surface ocean. Taxonomic diversity will be investigated by identifying the components of the phytoplankton assemblages using molecular, chemical and microscope methods. Genetic diversity will be explored at several levels, including direct sequencing of clone libraries of key functional genes and metatranscriptomic sequencing and microarray analysis of size fractionated/sorted phytoplankton assemblages. Using natural abundance and tracer stable isotope methods, genetic and taxonomic diversity will be linked to functional diversity in C and N assimilation in size- fractionated and taxon-sorted populations.</p>
DimBio NABE
largerWorkCitation
project
eng; USA
oceans
subarctic Atlantic Ocean
286.735
-19.904
35.547
59.83
2013-08-23
2014-05-17
North Atlantic Ocean, transects from southwest to northeast
0
BCO-DMO catalogue of parameters from Chlorophyll-a concentrations from CTD cast deployments and underway seawater inflow from Endeavor 532 and Endeavor 538 cruises in 2013 and 2014
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/651801.rdf
Name: cruise_id
Units: unitless
Description: <p>cruise identification</p>
http://lod.bco-dmo.org/id/dataset-parameter/651802.rdf
Name: cast
Units: unitless
Description: <p>cast number</p>
http://lod.bco-dmo.org/id/dataset-parameter/651803.rdf
Name: ISO_DateTime_UTC
Units: unitless
Description: <p>date and time of sampling in ISO8601 format (UTC) </p>
http://lod.bco-dmo.org/id/dataset-parameter/651804.rdf
Name: lon
Units: decimal degrees
Description: <p>longitude; east is positive</p>
http://lod.bco-dmo.org/id/dataset-parameter/651805.rdf
Name: lat
Units: decimal degrees
Description: <p>latitude; north is positive</p>
http://lod.bco-dmo.org/id/dataset-parameter/651806.rdf
Name: depth_w
Units: meters
Description: <p>depth of the water</p>
http://lod.bco-dmo.org/id/dataset-parameter/651807.rdf
Name: depth
Units: meters
Description: <p>depth</p>
http://lod.bco-dmo.org/id/dataset-parameter/651808.rdf
Name: Chla_2_20um
Units: nanogram/liter
Description: <p>chlorophyll a concentration in the larger than 2 µm and smaller than 20 µm size fraction</p>
http://lod.bco-dmo.org/id/dataset-parameter/651809.rdf
Name: Chla_2_20um_flag
Units: unitless
Description: <p>quality flag: 1=ok; 6=below detection limit; 9=no measurement</p>
http://lod.bco-dmo.org/id/dataset-parameter/651810.rdf
Name: Chla_gt_20um
Units: nanogram/liter
Description: <p>chlorophyll a concentration in the larger than 20 µm size fraction</p>
http://lod.bco-dmo.org/id/dataset-parameter/651811.rdf
Name: Chla_gt_20um_flag
Units: unitless
Description: <p>quality flag: 1=ok; 6=below detection limit; 9=no measurement</p>
http://lod.bco-dmo.org/id/dataset-parameter/651812.rdf
Name: Chla_lt_2um
Units: nanogram/liter
Description: <p>chlorophyll a concentration in the smaller than 2 µm size fraction</p>
http://lod.bco-dmo.org/id/dataset-parameter/651813.rdf
Name: Chla_lt_2um_flag
Units: unitless
Description: <p>quality flag: 1=ok; 6=below detection limit; 9=no measurement</p>
http://lod.bco-dmo.org/id/dataset-parameter/651814.rdf
Name: Chla_total
Units: nanogram/liter
Description: <p>sum of size-fractionated chlorophyll a concentration or total not size-fractionated chlorophyll a concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/651815.rdf
Name: Chla_total_flag
Units: unitless
Description: <p>quality flag: 1=ok; 6=below detection limit; 9=no measurement</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
21550
https://darchive.mblwhoilibrary.org/bitstream/1912/28643/1/dataset-651784_chlorophyll-en532-and-en538__v2.tsv
download
https://doi.org/10.26008/1912/bco-dmo.651784.2
download
onLine
dataset
<p>Discrete chlorophyll <em>a </em>(Chla) samples were measured fluorometrically (Holm-Hansen &amp; Riemann 1978) by Nicolas Van Oostende and Jessica Lueders Dumont at 6 depths spanning the surface, the bottom of the euphotic zone,&nbsp;the mixed layer, and Chla maximum depths. Up to 4 liters of seawater, retrieved directly from the Niskin bottles or the surface seawater inflow, were sequentially filtered through a 20 µm pore-size polycarbonate filter, a 2 µm pore-size polycarbonate filter, and a 0.3 µm pore-size glass fiber filter (GF-75; Sterlitech; 47 mm diameter). Filtrations were performed in the dark under low vacuum (&lt;200 mbar). The Chla filters were packaged into aluminium foil (GF filter), or a 5 ml cryovial, and immediately frozen at -80°C until analysis.</p>
<p>Chlorophyll was extracted in 90% acetone at 4°C overnight and measured using a Turner Trilogy fluorometer, calibrated against a pure Chla standard (<em>Anacystis nidulans</em> Chla, Sigma-Aldrich, Saint-Louis, USA). Measurements were corrected for the fluorescence of phaeopigments after acidification with HCl (24 mM final concentration). Limit of quantification was 1 µg per liter in extract, or approximately 1 ng per liter in the sample. The Chlorophyll <em>a</em> concentration of casts 15 to 41 was measured by James L. Pinckney using the HPLC method derived from the Van Heukelem et al. (1992, 1994) and Pinckney et al. (1996) protocols.</p>
Specified by the Principal Investigator(s)
<p>When measurements were below the limit of the quantification, the limit of quantification for that particular measurement is given and accompanied with a quality flag ("6"). 'nd' indicates no measurements were made with a quality flag (“9”).</p>
<p><strong>BCO-DMO Processing:</strong></p>
<p>-&nbsp;added conventional header with dataset name, PI name, version date<br />
- renamed parameters to BCO-DMO standard<br />
- replaced blank cells with nd<br />
- added depth_w to EN538 datasets so they'd match EN532</p>
<p>- replaced&nbsp;original 2016-07-13 EN532 data with new version submitted 2017-07-14. Cast numbers were rearranged.</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
PI Supplied Instrument Name: Instrument Name: CTD Sea-Bird SBE 911plus Instrument Short Name:CTD SBE 911plus Instrument Description: The Sea-Bird SBE 911 plus is a type of CTD instrument package for continuous measurement of conductivity, temperature and pressure. The SBE 911 plus includes the SBE 9plus Underwater Unit and the SBE 11plus Deck Unit (for real-time readout using conductive wire) for deployment from a vessel. The combination of the SBE 9 plus and SBE 11 plus is called a SBE 911 plus. The SBE 9 plus uses Sea-Bird's standard modular temperature and conductivity sensors (SBE 3 plus and SBE 4). The SBE 9 plus CTD can be configured with up to eight auxiliary sensors to measure other parameters including dissolved oxygen, pH, turbidity, fluorescence, light (PAR), light transmission, etc.). more information from Sea-Bird Electronics Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0058/
PI Supplied Instrument Name: PI Supplied Instrument Description:Turner Trilogy fluorometer Instrument Name: Fluorometer Instrument Short Name:Fluorometer Instrument Description: A fluorometer or fluorimeter is a device used to measure parameters of fluorescence: its intensity and wavelength distribution of emission spectrum after excitation by a certain spectrum of light. The instrument is designed to measure the amount of stimulated electromagnetic radiation produced by pulses of electromagnetic radiation emitted into a water sample or in situ. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/113/
PI Supplied Instrument Name: Instrument Name: High-Performance Liquid Chromatograph Instrument Short Name:HPLC Instrument Description: A High-performance liquid chromatograph (HPLC) is a type of liquid chromatography used to separate compounds that are dissolved in solution. HPLC instruments consist of a reservoir of the mobile phase, a pump, an injector, a separation column, and a detector. Compounds are separated by high pressure pumping of the sample mixture onto a column packed with microspheres coated with the stationary phase. The different components in the mixture pass through the column at different rates due to differences in their partitioning behavior between the mobile liquid phase and the stationary phase. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB11/
PI Supplied Instrument Name: Instrument Name: Niskin bottle Instrument Short Name:Niskin bottle Instrument Description: A Niskin bottle (a next generation water sampler based on the Nansen bottle) is a cylindrical, non-metallic water collection device with stoppers at both ends. The bottles can be attached individually on a hydrowire or deployed in 12, 24, or 36 bottle Rosette systems mounted on a frame and combined with a CTD. Niskin bottles are used to collect discrete water samples for a range of measurements including pigments, nutrients, plankton, etc. Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0412/
Cruise: EN532
EN532
R/V Endeavor
Community Standard Description
International Council for the Exploration of the Sea
R/V Endeavor
vessel
EN532
Bess B. Ward
Princeton University
http://dmoserv3.bco-dmo.org/data_docs/DimBio_NABE/EN532_CruiseReport.pdf
Report describing EN532
Cruise: EN538
EN538
R/V Endeavor
Community Standard Description
International Council for the Exploration of the Sea
R/V Endeavor
vessel
EN538
Bess B. Ward
Princeton University
http://dmoserv3.bco-dmo.org/data_docs/DimBio_NABE/EN538_CruiseReport.pdf
Report describing EN538
R/V Endeavor
Community Standard Description
International Council for the Exploration of the Sea
R/V Endeavor
vessel