http://lod.bco-dmo.org/id/dataset/651890
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2016-07-15
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Pico- and Nanoplankton concentrations from CTD cast deployments collected from the R/V Endeavor (EN532, EN538) cruises in the subarctic Atlantic Ocean from 2013-2014
2025-01-08
publication
2025-01-08
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2025-01-05
publication
https://doi.org/10.26008/1912/bco-dmo.651890.1
Bess B. Ward
Princeton University
principalInvestigator
Andrew E. Allen
J. Craig Venter Institute
principalInvestigator
Daniel M. Sigman
Princeton University
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Ward, B. B., Allen, A. E., Sigman, D. M. (2025) Pico- and Nanoplankton concentrations from CTD cast deployments collected from the R/V Endeavor (EN532, EN538) cruises in the subarctic Atlantic Ocean from 2013-2014. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 2) Version Date 2025-01-08 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.651890.2 [access date]
Pico- and Nanoplankton concentrations from ctd cast deployments on Endeavor 532 and Endeavor 538 Dataset Description: <p>This dataset includes pico- and nanoplankton cell concentrations from CTD casts made during the August-September 2013 EN532 and April-May 2014 EN358 cruises aboard R/V Endeavor. Study sites were located in the subarctic Atlantic Ocean along the 20 °W meridian between 50 °N and 60 °N in September 2013 and May 2014. Two transects from the US East coast to the subarctic study sites were performed as well.</p>
<p>See the "Related Datasets" section for several related BCO-DMO datasets from the same cruises.</p> Methods and Sampling: <p>The cell abundance of pico- and nanophytoplankton (&lt;~14 micrometers (µm) cell diameter) was determined by flow cytometric analysis of 1500 microliters (µl) of glutaraldehyde-preserved (1% v/v) (Marie et al. 1997) samples using a BD Accuri C6 flow cytometer equipped with a blue laser (488 nanometers (nm)), at a flow rate of 100 µl per minute, and a core diameter of 22 µm. Standard fluorescent bead solutions were prepared daily and used as an internal standard to assess instrument performance, to standardize scatter and fluorescence measurements (Rainbow Calibration Particles (8 peaks), BD Biosciences), and to validate the flow rate (TruCount, BD Biosciences) for quantitative applications. Each sample was run with fluorescent beads (YG beads, 0.94 µm Fluoresbrite® Yellow Green Microspheres, Polysciences, Inc.) as an internal standard for forward scatter measurements.</p>
<p>We distinguished several phytoplankton groups based on their forward (FSC) and side scatter (SSC), Chla, and phycoerythrin (PE) fluorescence signals: the picophytoplankton (&lt;~2.5 µm) group comprised PE-containing <em>Synechococcus </em>and non-PE-containing picoeukaryotes (picoEuk), the nanophytoplankton groups (nanoEuk, &gt;~2.5 - 14 µm) included PE-containing nanophytoplankton (PE_Euk), non-PE-containing phytoplankton, and coccolithophores (Cocco) (the latter group was identified based on their enhanced side scatter signal). The total concentration of nanoeukaryote phytoplankton (totnanoEuk) is the sum of PE_Euk, Cocco, and non-PE-containing phytoplankton other than Cocco.</p>
<p>The picoplanktonic <em>Prochlorococcus</em> cells were counted in SYBR Green I-stained samples (Marie et al. 1997), according to (Heywood et al. 2006), because of the difficulty of discriminating unstained cells from background noise. The concentration of heterotrophic bacterial cells was determined by flow cytometric analysis of 250 µl of glutaraldehyde-preserved (1% v/v) and SYBR Green I-stained (1:7500) samples according to Marie et al. (1997) and Gasol and Del Giorgio (2000). The <em>Prochlorococcus</em> and heterotrophic bacteria samples were analysed using a BD Accuri C6 flow cytometer, at a flow rate of 35 µl per minute, and a core diameter of 16 µm. All plankton groups were gated and their abundance quantified using FlowJo software (Tree Star, Inc., <a href="http://www.flowjo.com" target="_blank">www.flowjo.com</a>).</p>
<p>The biovolume of phytoplankton cells analyzed by flow cytometry was derived from forward scatter measurements of individual cells based on the polynomial relationship between the log10 of measured biovolumes of pico- and nanophytoplankton cells and the log10 of the peak area of their forward scatter signal (FSC-A) (Laney &amp; Sosik 2014). A calibration procedure, using bead stocks and an unidentified cultured picoeukaryote from the Sosik Lab at Woods Hole Oceanographic Institution, confirmed the inter-lab agreement of flow cytometry-derived biovolume estimates. Since the largest phytoplankton cell in the empirical relationship of Laney &amp; Sosik (2014) had a cell diameter of 14 µm and the number of cells larger than this in our samples was negligible, only cells up to ~14 µm in diameter were included in the cell abundance and biovolume calculations. Cellular biomass was estimated according to the relationship between cellular biovolume (cubic micrometers per cell (µm3 cell-1)) and carbon content (picomoles per cell (pmol cell-1)) for glutaraldehyde preserved pico- and nanophytoplankton cells from (Verity et al. 1992): C = (0.433 ⁄ 12) × biovolume^0.863.</p>
<p>Although <em>Synechococcus</em> cells could readily be counted based on their size and their characteristic PE fluorescence the high signal-to-noise ratio in the FSC-A channel of the Accuri precluded a reliable cell size estimate for particles smaller than 1 µm. Therefore, the biomass of <em>Synechococcus</em> was estimated using a conversion factor of 140 femtograms carbon per cell (fg C cell-1) assuming a cell diameter of 1 µm and 270 fg C µm-3 (Bertilsson et al. 2003). The biomass of <em>Prochlorococcus</em> cells was calculated by using an average cellular carbon content of 53.5 fg C cell-1 (Bertilsson et al. 2003), which is very similar to the range of cellular carbon content determined by Casey et al. (2013) for <em>Prochlorococcus</em> in the euphotic zone.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1136345 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1136345
completed
Bess B. Ward
Princeton University
609-258-5150
Geosciences Department 217 Guyot Hall
Princeton
NJ
08544-1003
USA
bbw@princeton.edu
pointOfContact
Andrew E. Allen
J. Craig Venter Institute
858-200-1826
4120 Capricorn Lane
La Jolla
CA
92037
USA
aallen@jcvi.org
pointOfContact
Daniel M. Sigman
Princeton University
609-258-2194
Department of Geosciences M52 Guyot Hall
Princeton
NJ
08544
USA
sigman@princeton.edu
pointOfContact
asNeeded
Dataset Version: 2
Unknown
cruise_id
cast
ISO_DateTime_UTC
lat
lon
lon_360
depth_w
depth
Syn_biomass_nMC
Syn_cells_per_ml
Syn_cells_per_ml_flag
picoEuk_biomass_nMC
picoEuk_cells_per_ml
picoEuk_cells_per_ml_flag
PE_Euk_biomass_nMC
PE_Euk_cells_per_ml
PE_Euk_cells_per_ml_flag
totnanoEuk_biomass_nMC
totnanoEuk_cells_per_ml
totnanoEuk_cells_per_ml_flag
cocco_biomass_nMC
cocco_cells_per_ml
cocco_cells_per_ml_flag
Prochlor_biomass_nMC
Prochlor_cells_per_ml
Prochlor_cells_per_ml_flag
total_FC_phyto_biomass_nMC
total_FC_phyto_cells_per_ml
total_FC_phyto_cells_per_ml_flag
total_het_bact_cells_per_ml
total_het_bact_cells_per_ml_flag
comment
theme
None, User defined
Cruise identfier
cast
ISO_DateTime_UTC
latitude
longitude
longitude from 0 to 360 degrees
depth_w
depth
biomass_C
synechococcus abundance
quality flag
pico_euks
nano eukaryotic phytoplankton
abundance
prochlorococcus abundance
comments
featureType
BCO-DMO Standard Parameters
CTD Sea-Bird SBE 911plus
Flow Cytometer
Niskin bottle
instrument
BCO-DMO Standard Instruments
EN532
EN538
service
Deployment Activity
subarctic Atlantic Ocean
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Dimensions of Biodiversity
http://www.nsf.gov/funding/pgm_summ.jsp?pims_id=503446
Dimensions of Biodiversity
(adapted from the NSF Synopsis of Program)
Dimensions of Biodiversity is a program solicitation from the NSF Directorate for Biological Sciences. FY 2010 was year one of the program. [MORE from NSF]
The NSF Dimensions of Biodiversity program seeks to characterize biodiversity on Earth by using integrative, innovative approaches to fill rapidly the most substantial gaps in our understanding. The program will take a broad view of biodiversity, and in its initial phase will focus on the integration of genetic, taxonomic, and functional dimensions of biodiversity. Project investigators are encouraged to integrate these three dimensions to understand the interactions and feedbacks among them. While this focus complements several core NSF programs, it differs by requiring that multiple dimensions of biodiversity be addressed simultaneously, to understand the roles of biodiversity in critical ecological and evolutionary processes.
Dimensions of Biodiversity
largerWorkCitation
program
Functional diversity of marine eukaryotic phytoplankton and their contributions to the C and N cycling
https://osprey.bco-dmo.org/project/544343
Functional diversity of marine eukaryotic phytoplankton and their contributions to the C and N cycling
<p>This project will investigate the taxonomic, genetic and functional diversity of eukaryotic phytoplankton at two North Atlantic sites (subarctic and subtropical) in two seasons. The PIs will use diagnostic microarrays for community analysis based on functional genes (both DNA and RNA) and next generation sequencing (i.e., transcriptomics using 454 technology) to identify the players, both in terms of community composition and activity, and to explore the functional diversity of the natural assemblage. In order to identify which groups are active in C and N assimilation and which N source is being utilized by the different size and functional groups, both filter-separated and flow cytometry-sorted samples will be used to 1) measure 13C primary production and 15N assimilation by incubations with isotope tracers, 2) measure the natural stable N isotope signatures of different taxonomic groups and 3) link the molecular diversity to the functional diversity in C and N transformations. Using flow cytometry linked to mass spectrometry, these investigators have found an unexpectedly strong differentiation in the form of N assimilated by prokaryotes and eukaryotes, with eukaryotes being more dynamic.</p>
<p>This project will investigate the taxonomic, genetic and functional diversity of eukaryotic phytoplankton and to link this diversity and assemblage composition to the carbon and nitrogen biogeochemistry of the surface ocean. Taxonomic diversity will be investigated by identifying the components of the phytoplankton assemblages using molecular, chemical and microscope methods. Genetic diversity will be explored at several levels, including direct sequencing of clone libraries of key functional genes and metatranscriptomic sequencing and microarray analysis of size fractionated/sorted phytoplankton assemblages. Using natural abundance and tracer stable isotope methods, genetic and taxonomic diversity will be linked to functional diversity in C and N assimilation in size- fractionated and taxon-sorted populations.</p>
DimBio NABE
largerWorkCitation
project
eng; USA
biota
oceans
subarctic Atlantic Ocean
-73.265
-19.988
35.547
60
2013-08-23
2014-05-20
North Atlantic Ocean, transects from southwest to northeast
0
BCO-DMO catalogue of parameters from Pico- and Nanoplankton concentrations from CTD cast deployments collected from the R/V Endeavor (EN532, EN538) cruises in the subarctic Atlantic Ocean from 2013-2014
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/948120.rdf
Name: cruise_id
Units: unitless
Description: <p>cruise identification</p>
http://lod.bco-dmo.org/id/dataset-parameter/948121.rdf
Name: cast
Units: unitless
Description: <p>cast number</p>
http://lod.bco-dmo.org/id/dataset-parameter/948122.rdf
Name: ISO_DateTime_UTC
Units: unitless
Description: <p>Date and time (UTC) in ISO 8601 format</p>
http://lod.bco-dmo.org/id/dataset-parameter/948123.rdf
Name: lat
Units: decimal degrees
Description: <p>latitude; positive values = North</p>
http://lod.bco-dmo.org/id/dataset-parameter/948124.rdf
Name: lon
Units: decimal degrees
Description: <p>longitude; negative values = West</p>
http://lod.bco-dmo.org/id/dataset-parameter/948125.rdf
Name: lon_360
Units: decimal degrees
Description: <p>longitude from 0 to 360 degrees</p>
http://lod.bco-dmo.org/id/dataset-parameter/948126.rdf
Name: depth_w
Units: meters
Description: <p>depth of the water</p>
http://lod.bco-dmo.org/id/dataset-parameter/948127.rdf
Name: depth
Units: meters
Description: <p>sample depth</p>
http://lod.bco-dmo.org/id/dataset-parameter/948128.rdf
Name: Syn_biomass_nMC
Units: nanomoles Carbon per liter (nmol C/liter)
Description: <p>concentration of Synechococcus carbon biomass</p>
http://lod.bco-dmo.org/id/dataset-parameter/948129.rdf
Name: Syn_cells_per_ml
Units: cells/milliliter
Description: <p>concentration of Synechococcus cells</p>
http://lod.bco-dmo.org/id/dataset-parameter/948130.rdf
Name: Syn_cells_per_ml_flag
Units: unitless
Description: <p>quality flag - see description in the Data Processing section of metadata</p>
http://lod.bco-dmo.org/id/dataset-parameter/948131.rdf
Name: picoEuk_biomass_nMC
Units: nanomoles Carbon per liter (nmol C/liter)
Description: <p>concentration of picoeukaryote phytoplankton carbon biomass</p>
http://lod.bco-dmo.org/id/dataset-parameter/948132.rdf
Name: picoEuk_cells_per_ml
Units: cells/milliliter
Description: <p>concentration of picoeukaryote phytoplankton cells</p>
http://lod.bco-dmo.org/id/dataset-parameter/948133.rdf
Name: picoEuk_cells_per_ml_flag
Units: unitless
Description: <p>quality flag - see description in the Data Processing section of metadata</p>
http://lod.bco-dmo.org/id/dataset-parameter/948134.rdf
Name: PE_Euk_biomass_nMC
Units: nanomoles Carbon per liter (nmol C/liter)
Description: <p>concentration of phycoerythrin-containing nanoeukaryote phytoplankton carbon biomass</p>
http://lod.bco-dmo.org/id/dataset-parameter/948135.rdf
Name: PE_Euk_cells_per_ml
Units: cells/milliliter
Description: <p>concentration of phycoerythrin-containing nanoeukaryote phytoplankton cells</p>
http://lod.bco-dmo.org/id/dataset-parameter/948136.rdf
Name: PE_Euk_cells_per_ml_flag
Units: unitless
Description: <p>quality flag - see description in the Data Processing section of metadata</p>
http://lod.bco-dmo.org/id/dataset-parameter/948137.rdf
Name: totnanoEuk_biomass_nMC
Units: nanomoles Carbon per liter (nmol C/liter)
Description: <p>concentration of nanoeukaryote phytoplankton carbon biomass</p>
http://lod.bco-dmo.org/id/dataset-parameter/948138.rdf
Name: totnanoEuk_cells_per_ml
Units: cells/milliliter
Description: <p>concentration of nanoeukaryote phytoplankton cells</p>
http://lod.bco-dmo.org/id/dataset-parameter/948139.rdf
Name: totnanoEuk_cells_per_ml_flag
Units: unitless
Description: <p>quality flag - see description in the Data Processing section of metadata</p>
http://lod.bco-dmo.org/id/dataset-parameter/948140.rdf
Name: cocco_biomass_nMC
Units: nanomoles Carbon per liter (nmol C/liter)
Description: <p>concentration of coccolithophore carbon biomass</p>
http://lod.bco-dmo.org/id/dataset-parameter/948141.rdf
Name: cocco_cells_per_ml
Units: cells/milliliter
Description: <p>concentration of coccolithophore cells</p>
http://lod.bco-dmo.org/id/dataset-parameter/948142.rdf
Name: cocco_cells_per_ml_flag
Units: unitless
Description: <p>quality flag - see description in the Data Processing section of metadata</p>
http://lod.bco-dmo.org/id/dataset-parameter/948143.rdf
Name: Prochlor_biomass_nMC
Units: nanomoles Carbon per liter (nmol C/liter)
Description: <p>concentration of Prochlorococcus carbon biomass</p>
http://lod.bco-dmo.org/id/dataset-parameter/948144.rdf
Name: Prochlor_cells_per_ml
Units: cells/milliliter
Description: <p>concentration of Prochlorococcus cells</p>
http://lod.bco-dmo.org/id/dataset-parameter/948145.rdf
Name: Prochlor_cells_per_ml_flag
Units: unitless
Description: <p>quality flag - see description in the Data Processing section of metadata</p>
http://lod.bco-dmo.org/id/dataset-parameter/948146.rdf
Name: total_FC_phyto_biomass_nMC
Units: nanomoles Carbon per liter (nmol C/liter)
Description: <p>concentration of total flow cytometrically determined phytoplankton carbon biomass</p>
http://lod.bco-dmo.org/id/dataset-parameter/948147.rdf
Name: total_FC_phyto_cells_per_ml
Units: cells/milliliter
Description: <p>concentration of total flow cytometrically determined phytoplankton cells</p>
http://lod.bco-dmo.org/id/dataset-parameter/948148.rdf
Name: total_FC_phyto_cells_per_ml_flag
Units: unitless
Description: <p>quality flag - see description in the Data Processing section of metadata</p>
http://lod.bco-dmo.org/id/dataset-parameter/948149.rdf
Name: total_het_bact_cells_per_ml
Units: cells/milliliter
Description: <p>concentration of non-chlorophyll a containing bacterial cells</p>
http://lod.bco-dmo.org/id/dataset-parameter/948150.rdf
Name: total_het_bact_cells_per_ml_flag
Units: unitless
Description: <p>quality flag - see description in the Data Processing section of metadata</p>
http://lod.bco-dmo.org/id/dataset-parameter/948151.rdf
Name: comment
Units: unitless
Description: <p>comments</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
35540
https://darchive.mblwhoilibrary.org/server/api/core/bitstreams/6c279b02-45fc-432d-a6b4-5bdccba504e9/content
download
37399
https://darchive.mblwhoilibrary.org/server/api/core/bitstreams/3f3d6cb4-a3ac-4578-8e82-0507ff978ea9/content
download
https://doi.org/10.26008/1912/bco-dmo.651890.1
download
onLine
dataset
<p>The cell abundance of pico- and nanophytoplankton (&lt;~14 micrometers (µm) cell diameter) was determined by flow cytometric analysis of 1500 microliters (µl) of glutaraldehyde-preserved (1% v/v) (Marie et al. 1997) samples using a BD Accuri C6 flow cytometer equipped with a blue laser (488 nanometers (nm)), at a flow rate of 100 µl per minute, and a core diameter of 22 µm. Standard fluorescent bead solutions were prepared daily and used as an internal standard to assess instrument performance, to standardize scatter and fluorescence measurements (Rainbow Calibration Particles (8 peaks), BD Biosciences), and to validate the flow rate (TruCount, BD Biosciences) for quantitative applications. Each sample was run with fluorescent beads (YG beads, 0.94 µm Fluoresbrite® Yellow Green Microspheres, Polysciences, Inc.) as an internal standard for forward scatter measurements.</p>
<p>We distinguished several phytoplankton groups based on their forward (FSC) and side scatter (SSC), Chla, and phycoerythrin (PE) fluorescence signals: the picophytoplankton (&lt;~2.5 µm) group comprised PE-containing <em>Synechococcus </em>and non-PE-containing picoeukaryotes (picoEuk), the nanophytoplankton groups (nanoEuk, &gt;~2.5 - 14 µm) included PE-containing nanophytoplankton (PE_Euk), non-PE-containing phytoplankton, and coccolithophores (Cocco) (the latter group was identified based on their enhanced side scatter signal). The total concentration of nanoeukaryote phytoplankton (totnanoEuk) is the sum of PE_Euk, Cocco, and non-PE-containing phytoplankton other than Cocco.</p>
<p>The picoplanktonic <em>Prochlorococcus</em> cells were counted in SYBR Green I-stained samples (Marie et al. 1997), according to (Heywood et al. 2006), because of the difficulty of discriminating unstained cells from background noise. The concentration of heterotrophic bacterial cells was determined by flow cytometric analysis of 250 µl of glutaraldehyde-preserved (1% v/v) and SYBR Green I-stained (1:7500) samples according to Marie et al. (1997) and Gasol and Del Giorgio (2000). The <em>Prochlorococcus</em> and heterotrophic bacteria samples were analysed using a BD Accuri C6 flow cytometer, at a flow rate of 35 µl per minute, and a core diameter of 16 µm. All plankton groups were gated and their abundance quantified using FlowJo software (Tree Star, Inc., <a href="http://www.flowjo.com" target="_blank">www.flowjo.com</a>).</p>
<p>The biovolume of phytoplankton cells analyzed by flow cytometry was derived from forward scatter measurements of individual cells based on the polynomial relationship between the log10 of measured biovolumes of pico- and nanophytoplankton cells and the log10 of the peak area of their forward scatter signal (FSC-A) (Laney &amp; Sosik 2014). A calibration procedure, using bead stocks and an unidentified cultured picoeukaryote from the Sosik Lab at Woods Hole Oceanographic Institution, confirmed the inter-lab agreement of flow cytometry-derived biovolume estimates. Since the largest phytoplankton cell in the empirical relationship of Laney &amp; Sosik (2014) had a cell diameter of 14 µm and the number of cells larger than this in our samples was negligible, only cells up to ~14 µm in diameter were included in the cell abundance and biovolume calculations. Cellular biomass was estimated according to the relationship between cellular biovolume (cubic micrometers per cell (µm3 cell-1)) and carbon content (picomoles per cell (pmol cell-1)) for glutaraldehyde preserved pico- and nanophytoplankton cells from (Verity et al. 1992): C = (0.433 ⁄ 12) × biovolume^0.863.</p>
<p>Although <em>Synechococcus</em> cells could readily be counted based on their size and their characteristic PE fluorescence the high signal-to-noise ratio in the FSC-A channel of the Accuri precluded a reliable cell size estimate for particles smaller than 1 µm. Therefore, the biomass of <em>Synechococcus</em> was estimated using a conversion factor of 140 femtograms carbon per cell (fg C cell-1) assuming a cell diameter of 1 µm and 270 fg C µm-3 (Bertilsson et al. 2003). The biomass of <em>Prochlorococcus</em> cells was calculated by using an average cellular carbon content of 53.5 fg C cell-1 (Bertilsson et al. 2003), which is very similar to the range of cellular carbon content determined by Casey et al. (2013) for <em>Prochlorococcus</em> in the euphotic zone.</p>
Specified by the Principal Investigator(s)
<p><strong>Quality Flags:</strong><br />
The data quality flag scheme used is that of SEADATANET, where:<br />
no quality control = 0<br />
good value = 1<br />
probably good value = 2<br />
probably bad value = 3<br />
bad value = 4<br />
changed value = 5<br />
value below detection = 6<br />
value in excess = 7<br />
interpolated value = 8<br />
missing value = 9<br />
value phenomenon uncertain = A</p>
<p><strong>BCO-DMO Processing &amp; Version History:<br />
Version 1 (date: 2016-07-15)</strong><br />
- added conventional header with dataset name, PI name, version date.<br />
- renamed parameters to BCO-DMO standard.<br />
- replaced blank cells with "nd".<br />
- replaced blanks with underscores.<br />
- formatted lat and long to 4 decimal places.<br />
Version 1 contained some incorrect values in column "totnanoEuk_biomass_nMC". These values have been corrected in version 2.</p>
<p><strong>Version 2 (date: 2025-01-08)</strong><br />
- Imported original file "FCM_Ward_Dimensions_2024.xlsx" into the BCO-DMO system.<br />
- Marked "nd" as a missing data identifier; all missing data are empty/blank in the final csv file.<br />
- Added a trailing "Z" to the ISO_DateTime_UTC column to indicate UTC time zone.<br />
- Added column for longitude in -180&nbsp;to 180 degrees; renamed the original longitude column "lon_360"<br />
- Saved the final file as "651890_v2_pico_nano_abund_fcm_en532_en538.csv".<br />
<strong>In this version, values in the column "totnanoEuk_biomass_nMC" have been corrected.</strong></p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
PI Supplied Instrument Name: Instrument Name: CTD Sea-Bird SBE 911plus Instrument Short Name:CTD SBE 911plus Instrument Description: The Sea-Bird SBE 911 plus is a type of CTD instrument package for continuous measurement of conductivity, temperature and pressure. The SBE 911 plus includes the SBE 9plus Underwater Unit and the SBE 11plus Deck Unit (for real-time readout using conductive wire) for deployment from a vessel. The combination of the SBE 9 plus and SBE 11 plus is called a SBE 911 plus. The SBE 9 plus uses Sea-Bird's standard modular temperature and conductivity sensors (SBE 3 plus and SBE 4). The SBE 9 plus CTD can be configured with up to eight auxiliary sensors to measure other parameters including dissolved oxygen, pH, turbidity, fluorescence, light (PAR), light transmission, etc.). more information from Sea-Bird Electronics Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0058/
PI Supplied Instrument Name: PI Supplied Instrument Description:BD Accuri C6 flow cytometer Instrument Name: Flow Cytometer Instrument Short Name:Flow Cytometer Instrument Description: Flow cytometers (FC or FCM) are automated instruments that quantitate properties of single cells, one cell at a time. They can measure cell size, cell granularity, the amounts of cell components such as total DNA, newly synthesized DNA, gene expression as the amount messenger RNA for a particular gene, amounts of specific surface receptors, amounts of intracellular proteins, or transient signalling events in living cells.
(from: http://www.bio.umass.edu/micro/immunology/facs542/facswhat.htm) Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB37/
PI Supplied Instrument Name: Instrument Name: Niskin bottle Instrument Short Name:Niskin bottle Instrument Description: A Niskin bottle (a next generation water sampler based on the Nansen bottle) is a cylindrical, non-metallic water collection device with stoppers at both ends. The bottles can be attached individually on a hydrowire or deployed in 12, 24, or 36 bottle Rosette systems mounted on a frame and combined with a CTD. Niskin bottles are used to collect discrete water samples for a range of measurements including pigments, nutrients, plankton, etc. Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0412/
Cruise: EN532
EN532
R/V Endeavor
Community Standard Description
International Council for the Exploration of the Sea
R/V Endeavor
vessel
EN532
Bess B. Ward
Princeton University
http://dmoserv3.bco-dmo.org/data_docs/DimBio_NABE/EN532_CruiseReport.pdf
Report describing EN532
Cruise: EN538
EN538
R/V Endeavor
Community Standard Description
International Council for the Exploration of the Sea
R/V Endeavor
vessel
EN538
Bess B. Ward
Princeton University
http://dmoserv3.bco-dmo.org/data_docs/DimBio_NABE/EN538_CruiseReport.pdf
Report describing EN538
R/V Endeavor
Community Standard Description
International Council for the Exploration of the Sea
R/V Endeavor
vessel