http://lod.bco-dmo.org/id/dataset/652025
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2016-07-15
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Particulate nitrogen concentrations, N isotopic composition, and nitrate isotopic composition from EN532
2016-07-15
publication
2016-07-15
revision
BCO-DMO Linked Data URI
2016-07-15
creation
http://lod.bco-dmo.org/id/dataset/652025
Bess B. Ward
Princeton University
principalInvestigator
Andrew E. Allen
J. Craig Venter Institute
principalInvestigator
Daniel M. Sigman
Princeton University
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Ward, B. B., Allen, A. E., Sigman, D. M. (2016) Particulate nitrogen concentrations, N isotopic composition, and nitrate isotopic composition from EN532. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 2) Version Date 2016-07-15 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/652025 [access date]
Particulate nitrogen concentrations and N isotopic composition, and nitrate isotopic composition from EN532 Dataset Description: <p>Pico- and nanoplankton cell concentrations&nbsp;from&nbsp;CTD casts made during the August-September 2013 EN532 and April-May 2014 EN358 cruises aboard R/V Endeavor. Study sites in the subarctic Atlantic Ocean along the 20 °W meridian between 50 °N and 60 °N in September 2013 and May 2014. Two transects from the US East coast to the subarctic study sites were performed as well.</p>
<p><strong>Related Dataset:</strong><br />
<a href="http://www.bco-dmo.org/dataset/563684" target="_blank">EN532 - CTD</a><br />
<a href="http://www.bco-dmo.org/dataset/564097" target="_blank">EN538 - CTD</a><br />
<a href="http://www.bco-dmo.org/dataset/651784" target="_blank">Chlorophyll-a: EN532 and EN538</a><br />
<a href="http://www.bco-dmo.org/dataset/651816" target="_blank">Nutrients: EN532 and EN538</a><br />
<a href="http://www.bco-dmo.org/dataset/651890" target="_blank">FCM: EN532 and EN538</a></p> Methods and Sampling: <p>Seawater samples for analysis of the N isotopic composition of nitrate+nitrite and nitrate-only were collected unfiltered at regular depth intervals from the surface to 1000 m in 60 ml (&gt;150 m) or 125 ml (&lt;150 m) square-bottomed, wide-mouth HDPE bottles (Nalgene). Bottles were acid-washed and rinsed with deionized water prior to sampling. At sea, pre-labelled bottles and caps were rinsed three times with sample water, filled to ~85% of the bottle volume, and frozen upright at -20°C until analysis.</p>
<p>Isotopic analyses were conducted using the “denitrifier method”, wherein denitrifying bacteria lacking nitrous oxide (N2O) reductase quantitatively convert nitrate and nitrite in the sample to N2O gas (Sigman et al. 2001, Casciotti et al. 2002) (see also (Weigand et al. in review) for the updated protocol used for analyzing these samples). The isotopic composition of N2O was then measured by gas chromatography-isotope ratio mass spectrometry (GC-IRMS) using a purpose-built on-line N2O extraction and purification system and a Thermo MAT 253 mass spectrometer. Seawater solutions of the international nitrate reference materials, IAEA-N3 and USGS34, as well as an in-house N2O standard, were run in parallel to the samples in order to monitor the quality of bacterial N conversion and mass spectrometric measurements. The reference materials bracketed each group of ~10 samples and were used to correct the measured δ15N to N2 in air (Sigman et al. 2001, Casciotti et al. 2002, McIlvin &amp; Casciotti 2011). &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</p>
<p>The measurement of the 15N of nitrate-only for samples with a detectable concentration of nitrite required a nitrite removal pre-treatment. The detection limit for nitrite in this case was 2 nmol kg-1. Samples collected between the surface and ~125 m were treated for nitrite removal via the addition of 10 µl of sulphamic acid solution per ml of sample, which converts sample nitrite to N2 gas with a reaction time of 2-8 minutes, followed by the addition of 5.5 µl of 2M NaOH per ml of sample to restore the pH of the sample to ~7-9 (Granger &amp; Sigman 2009). The pooled standard error for 15N was 0.04‰ and 0.11‰ (n ≥3) for nitrate+nitrite and nitrate concentrations ≥0.5 µmol l-1 and &lt;0.5 µmol l-1, respectively. Hereafter, “nitrate” in the text refers to nitrate-only, after the subtraction (for concentration) or removal (for 15N) of nitrite.</p>
<p>Suspended particulate N: Suspended PN was collected at various depths throughout the euphotic zone, including within the surface mixed layer and at the depth of maximum chlorophyll concentration, by gentle vacuum filtration (&lt;135 mbar), of 8 l of seawater through a GF-75 filter. Filters were transferred to pre-combusted (500°C for 5 h) aluminium foil envelopes, and immediately frozen at -80°C until analysis. In the laboratory, the PN filters were dried in a desiccating oven at 40°C. Three subsamples were cored from each filter and transferred to combusted 4 mL glass Wheaton vials. PN was oxidised to nitrate using the persulphate oxidation method of Knapp et al. (2005), and as modified by Fawcett et al. (2011; 2014); this was conducted in a laminar flow hood equipped with an ammonia/amine filter. Briefly, 2 ml of persulphate oxidizing reagent (POR) were added to each sample vial, as well as to triplicate vials containing a filter blank plus varying quantities of two L-glutamic acid isotope standards, USGS-40 and USGS-41 (Qi et al. 2003); this allows determination of the N content and 15N of the POR+filter blank. The POR was made by dissolving 2.5 g of 4× recrystallised, methanol-rinsed potassium persulphate and 2.5 g of sodium hydroxide in 100 ml of ultra high-purity deionised water. Following POR addition, vials were autoclaved at 121°C for 55 minutes on a slow-vent setting, after which sample pH was lowered to 5-8 using 12N HCl. The concentration and δ15N of the resultant nitrate was measured via chemiluminescent analysis (Braman &amp; Hendrix 1989) and the denitrifier method (see above) (Sigman et al. 2001, Casciotti et al. 2002). The final N content and δ15N of the oxidised samples was corrected for the POR+filter blank. N content was converted to PN concentration by normalising to whole-filter area and volume of seawater filtered.</p>
<p>References:</p>
<p>Braman RS, Hendrix SA (1989) Nanogram nitrite and nitrate determination in environmental and biological materials by vanadium(iii) reduction with chemi-luminescence detection. Anal Chem 61:2715-2718</p>
<p>Casciotti K, Sigman D, Hastings MG, Böhlke J, Hilkert A (2002) Measurement of the oxygen isotopic composition of nitrate in seawater and freshwater using the denitrifier method. Anal Chem 74:4905-4912</p>
<p>Fawcett SE, Lomas M, Casey JR, Ward BB, Sigman DM (2011) Assimilation of upwelled nitrate by small eukaryotes in the Sargasso Sea. Nature Geoscience 4:717-722</p>
<p>Fawcett SE, Lomas MW, Ward BB, Sigman DM (2014) The counterintuitive effect of summer‐to‐fall mixed layer deepening on eukaryotic new production in the Sargasso Sea. Glob Biogeochem Cycle 28:86-102</p>
<p>Granger J, Sigman DM (2009) Removal of nitrite with sulfamic acid for nitrate N and O isotope analysis with the denitrifier method. Rapid Commun Mass Spectrom 23:3753-3762</p>
<p>Knapp AN, Sigman DM, Lipschultz F (2005) N isotopic composition of dissolved organic nitrogen and nitrate at the Bermuda Atlantic Time‐series Study site. Glob Biogeochem Cycle 19</p>
<p>McIlvin MR, Casciotti KL (2011) Technical updates to the bacterial method for nitrate isotopic analyses. Anal Chem 83:1850-1856</p>
<p>Qi H, Coplen TB, Geilmann H, Brand WA, Böhlke J (2003) Two new organic reference materials for δ13C and δ15N measurements and a new value for the δ13C of NBS 22 oil. Rapid Commun Mass Spectrom 17:2483-2487</p>
<p>Sigman D, Casciotti K, Andreani M, Barford C, Galanter M, Böhlke J (2001) A bacterial method for the nitrogen isotopic analysis of nitrate in seawater and freshwater. Anal Chem 73:4145-4153</p>
<p>Weigand MA, Foriel J, Barnett B, Oleynik S, Sigman DM (in review) Updates to instrumentation and protocols for isotopic analysis of nitrate by the denitrifier method. Rapid Commun Mass Spectrom</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1136345 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1136345
completed
Bess B. Ward
Princeton University
609-258-5150
Geosciences Department 217 Guyot Hall
Princeton
NJ
08544-1003
USA
bbw@princeton.edu
pointOfContact
Andrew E. Allen
J. Craig Venter Institute
858-200-1826
4120 Capricorn Lane
La Jolla
CA
92037
USA
aallen@jcvi.org
pointOfContact
Daniel M. Sigman
Princeton University
609-258-2194
Department of Geosciences M52 Guyot Hall
Princeton
NJ
08544
USA
sigman@princeton.edu
pointOfContact
asNeeded
Dataset Version: 2
Unknown
cruise_id
cast
ISO_DateTime_UTC
lat
lon
depth
PN_muM
SD_PN_muM
d15NPN_permil
SDd15NPN_permil
d15NNO3NO2_permil
SDd15NNO3NO2_permil
d18ONO3NO2_permil
SDd18ONO3NO2_permil
d15NNO3_permil
SDd15NNO3_permil
d18ONO3_permil
SDd18ONO3_permil
GC-IRMS
theme
None, User defined
Cruise identfier
cast
ISO_DateTime_UTC
latitude
longitude
depth
No BCO-DMO term
standard deviation
featureType
BCO-DMO Standard Parameters
CTD Sea-Bird SBE 911plus
Isotope-ratio Mass Spectrometer
Niskin bottle
instrument
BCO-DMO Standard Instruments
EN532
service
Deployment Activity
subarctic Atlantic Ocean
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Dimensions of Biodiversity
http://www.nsf.gov/funding/pgm_summ.jsp?pims_id=503446
Dimensions of Biodiversity
(adapted from the NSF Synopsis of Program)
Dimensions of Biodiversity is a program solicitation from the NSF Directorate for Biological Sciences. FY 2010 was year one of the program. [MORE from NSF]
The NSF Dimensions of Biodiversity program seeks to characterize biodiversity on Earth by using integrative, innovative approaches to fill rapidly the most substantial gaps in our understanding. The program will take a broad view of biodiversity, and in its initial phase will focus on the integration of genetic, taxonomic, and functional dimensions of biodiversity. Project investigators are encouraged to integrate these three dimensions to understand the interactions and feedbacks among them. While this focus complements several core NSF programs, it differs by requiring that multiple dimensions of biodiversity be addressed simultaneously, to understand the roles of biodiversity in critical ecological and evolutionary processes.
Dimensions of Biodiversity
largerWorkCitation
program
Functional diversity of marine eukaryotic phytoplankton and their contributions to the C and N cycling
https://osprey.bco-dmo.org/project/544343
Functional diversity of marine eukaryotic phytoplankton and their contributions to the C and N cycling
<p>This project will investigate the taxonomic, genetic and functional diversity of eukaryotic phytoplankton at two North Atlantic sites (subarctic and subtropical) in two seasons. The PIs will use diagnostic microarrays for community analysis based on functional genes (both DNA and RNA) and next generation sequencing (i.e., transcriptomics using 454 technology) to identify the players, both in terms of community composition and activity, and to explore the functional diversity of the natural assemblage. In order to identify which groups are active in C and N assimilation and which N source is being utilized by the different size and functional groups, both filter-separated and flow cytometry-sorted samples will be used to 1) measure 13C primary production and 15N assimilation by incubations with isotope tracers, 2) measure the natural stable N isotope signatures of different taxonomic groups and 3) link the molecular diversity to the functional diversity in C and N transformations. Using flow cytometry linked to mass spectrometry, these investigators have found an unexpectedly strong differentiation in the form of N assimilated by prokaryotes and eukaryotes, with eukaryotes being more dynamic.</p>
<p>This project will investigate the taxonomic, genetic and functional diversity of eukaryotic phytoplankton and to link this diversity and assemblage composition to the carbon and nitrogen biogeochemistry of the surface ocean. Taxonomic diversity will be investigated by identifying the components of the phytoplankton assemblages using molecular, chemical and microscope methods. Genetic diversity will be explored at several levels, including direct sequencing of clone libraries of key functional genes and metatranscriptomic sequencing and microarray analysis of size fractionated/sorted phytoplankton assemblages. Using natural abundance and tracer stable isotope methods, genetic and taxonomic diversity will be linked to functional diversity in C and N assimilation in size- fractionated and taxon-sorted populations.</p>
DimBio NABE
largerWorkCitation
project
eng; USA
oceans
subarctic Atlantic Ocean
2016-07-15
North Atlantic Ocean, transects from southwest to northeast
0
BCO-DMO catalogue of parameters from Particulate nitrogen concentrations, N isotopic composition, and nitrate isotopic composition from EN532
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/652043.rdf
Name: cruise_id
Units: unitless
Description: <p>cruise identification</p>
http://lod.bco-dmo.org/id/dataset-parameter/652044.rdf
Name: cast
Units: unitless
Description: <p>cast number</p>
http://lod.bco-dmo.org/id/dataset-parameter/652045.rdf
Name: ISO_DateTime_UTC
Units: yrday_utc
Description: <p>UTC day and decimal time; as 326.5 for the 326th day of the year or November 22 at 1200 hours (noon).</p>
http://lod.bco-dmo.org/id/dataset-parameter/652046.rdf
Name: lat
Units: decimal degrees
Description: <p>latitude; north is positive</p>
http://lod.bco-dmo.org/id/dataset-parameter/652047.rdf
Name: lon
Units: decimal degrees
Description: <p>longitude; east is positive</p>
http://lod.bco-dmo.org/id/dataset-parameter/652048.rdf
Name: depth
Units: meters
Description: <p>depth</p>
http://lod.bco-dmo.org/id/dataset-parameter/652049.rdf
Name: PN_muM
Units: umol/liter
Description: <p>particulate nitrogen concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/652050.rdf
Name: SD_PN_muM
Units: umol/liter
Description: <p>standard deviation of particulate nitrogen concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/652051.rdf
Name: d15NPN_permil
Units: per mille
Description: <p>delta 15N vs. atmospheric N2 of particulate nitrogen</p>
http://lod.bco-dmo.org/id/dataset-parameter/652052.rdf
Name: SDd15NPN_permil
Units: per mille
Description: <p>standard deviation of delta 15N vs. atmospheric N2 of particulate nitrogen</p>
http://lod.bco-dmo.org/id/dataset-parameter/652053.rdf
Name: d15NNO3NO2_permil
Units: per mille
Description: <p>delta 15N vs. atmospheric N2 of nitrate+nitrite</p>
http://lod.bco-dmo.org/id/dataset-parameter/652054.rdf
Name: SDd15NNO3NO2_permil
Units: per mille
Description: <p>standard deviation of delta 15N vs. atmospheric N2 of nitrate+nitrite</p>
http://lod.bco-dmo.org/id/dataset-parameter/652055.rdf
Name: d18ONO3NO2_permil
Units: per mille
Description: <p>delta 18O vs. VSMOW of nitrate+nitrite</p>
http://lod.bco-dmo.org/id/dataset-parameter/652056.rdf
Name: SDd18ONO3NO2_permil
Units: per mille
Description: <p>standard deviation of delta 18O vs. VSMOW of nitrate+nitrite</p>
http://lod.bco-dmo.org/id/dataset-parameter/652057.rdf
Name: d15NNO3_permil
Units: per mille
Description: <p>delta 15N vs. atmospheric N2 of nitrate</p>
http://lod.bco-dmo.org/id/dataset-parameter/652058.rdf
Name: SDd15NNO3_permil
Units: per mille
Description: <p>standard deviation of delta 15N vs. atmospheric N2 of nitrate</p>
http://lod.bco-dmo.org/id/dataset-parameter/652059.rdf
Name: d18ONO3_permil
Units: per mille
Description: <p>delta 18O vs. VSMOW of nitrate</p>
http://lod.bco-dmo.org/id/dataset-parameter/652060.rdf
Name: SDd18ONO3_permil
Units: per mille
Description: <p>standard deviation of delta 18O vs. VSMOW of nitrate</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
23507
https://datadocs.bco-dmo.org/file/p99nDrWiOnzjM7/EN532_PN_NO3_isotopes.csv
EN532_PN_NO3_isotopes.csv
Primary data file for dataset ID 652025
download
https://osprey.bco-dmo.org/dataset/652025/data/download
download
onLine
dataset
<p>Seawater samples for analysis of the N isotopic composition of nitrate+nitrite and nitrate-only were collected unfiltered at regular depth intervals from the surface to 1000 m in 60 ml (&gt;150 m) or 125 ml (&lt;150 m) square-bottomed, wide-mouth HDPE bottles (Nalgene). Bottles were acid-washed and rinsed with deionized water prior to sampling. At sea, pre-labelled bottles and caps were rinsed three times with sample water, filled to ~85% of the bottle volume, and frozen upright at -20°C until analysis.</p>
<p>Isotopic analyses were conducted using the “denitrifier method”, wherein denitrifying bacteria lacking nitrous oxide (N2O) reductase quantitatively convert nitrate and nitrite in the sample to N2O gas (Sigman et al. 2001, Casciotti et al. 2002) (see also (Weigand et al. in review) for the updated protocol used for analyzing these samples). The isotopic composition of N2O was then measured by gas chromatography-isotope ratio mass spectrometry (GC-IRMS) using a purpose-built on-line N2O extraction and purification system and a Thermo MAT 253 mass spectrometer. Seawater solutions of the international nitrate reference materials, IAEA-N3 and USGS34, as well as an in-house N2O standard, were run in parallel to the samples in order to monitor the quality of bacterial N conversion and mass spectrometric measurements. The reference materials bracketed each group of ~10 samples and were used to correct the measured δ15N to N2 in air (Sigman et al. 2001, Casciotti et al. 2002, McIlvin &amp; Casciotti 2011). &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</p>
<p>The measurement of the 15N of nitrate-only for samples with a detectable concentration of nitrite required a nitrite removal pre-treatment. The detection limit for nitrite in this case was 2 nmol kg-1. Samples collected between the surface and ~125 m were treated for nitrite removal via the addition of 10 µl of sulphamic acid solution per ml of sample, which converts sample nitrite to N2 gas with a reaction time of 2-8 minutes, followed by the addition of 5.5 µl of 2M NaOH per ml of sample to restore the pH of the sample to ~7-9 (Granger &amp; Sigman 2009). The pooled standard error for 15N was 0.04‰ and 0.11‰ (n ≥3) for nitrate+nitrite and nitrate concentrations ≥0.5 µmol l-1 and &lt;0.5 µmol l-1, respectively. Hereafter, “nitrate” in the text refers to nitrate-only, after the subtraction (for concentration) or removal (for 15N) of nitrite.</p>
<p>Suspended particulate N: Suspended PN was collected at various depths throughout the euphotic zone, including within the surface mixed layer and at the depth of maximum chlorophyll concentration, by gentle vacuum filtration (&lt;135 mbar), of 8 l of seawater through a GF-75 filter. Filters were transferred to pre-combusted (500°C for 5 h) aluminium foil envelopes, and immediately frozen at -80°C until analysis. In the laboratory, the PN filters were dried in a desiccating oven at 40°C. Three subsamples were cored from each filter and transferred to combusted 4 mL glass Wheaton vials. PN was oxidised to nitrate using the persulphate oxidation method of Knapp et al. (2005), and as modified by Fawcett et al. (2011; 2014); this was conducted in a laminar flow hood equipped with an ammonia/amine filter. Briefly, 2 ml of persulphate oxidizing reagent (POR) were added to each sample vial, as well as to triplicate vials containing a filter blank plus varying quantities of two L-glutamic acid isotope standards, USGS-40 and USGS-41 (Qi et al. 2003); this allows determination of the N content and 15N of the POR+filter blank. The POR was made by dissolving 2.5 g of 4× recrystallised, methanol-rinsed potassium persulphate and 2.5 g of sodium hydroxide in 100 ml of ultra high-purity deionised water. Following POR addition, vials were autoclaved at 121°C for 55 minutes on a slow-vent setting, after which sample pH was lowered to 5-8 using 12N HCl. The concentration and δ15N of the resultant nitrate was measured via chemiluminescent analysis (Braman &amp; Hendrix 1989) and the denitrifier method (see above) (Sigman et al. 2001, Casciotti et al. 2002). The final N content and δ15N of the oxidised samples was corrected for the POR+filter blank. N content was converted to PN concentration by normalising to whole-filter area and volume of seawater filtered.</p>
<p>References:</p>
<p>Braman RS, Hendrix SA (1989) Nanogram nitrite and nitrate determination in environmental and biological materials by vanadium(iii) reduction with chemi-luminescence detection. Anal Chem 61:2715-2718</p>
<p>Casciotti K, Sigman D, Hastings MG, Böhlke J, Hilkert A (2002) Measurement of the oxygen isotopic composition of nitrate in seawater and freshwater using the denitrifier method. Anal Chem 74:4905-4912</p>
<p>Fawcett SE, Lomas M, Casey JR, Ward BB, Sigman DM (2011) Assimilation of upwelled nitrate by small eukaryotes in the Sargasso Sea. Nature Geoscience 4:717-722</p>
<p>Fawcett SE, Lomas MW, Ward BB, Sigman DM (2014) The counterintuitive effect of summer‐to‐fall mixed layer deepening on eukaryotic new production in the Sargasso Sea. Glob Biogeochem Cycle 28:86-102</p>
<p>Granger J, Sigman DM (2009) Removal of nitrite with sulfamic acid for nitrate N and O isotope analysis with the denitrifier method. Rapid Commun Mass Spectrom 23:3753-3762</p>
<p>Knapp AN, Sigman DM, Lipschultz F (2005) N isotopic composition of dissolved organic nitrogen and nitrate at the Bermuda Atlantic Time‐series Study site. Glob Biogeochem Cycle 19</p>
<p>McIlvin MR, Casciotti KL (2011) Technical updates to the bacterial method for nitrate isotopic analyses. Anal Chem 83:1850-1856</p>
<p>Qi H, Coplen TB, Geilmann H, Brand WA, Böhlke J (2003) Two new organic reference materials for δ13C and δ15N measurements and a new value for the δ13C of NBS 22 oil. Rapid Commun Mass Spectrom 17:2483-2487</p>
<p>Sigman D, Casciotti K, Andreani M, Barford C, Galanter M, Böhlke J (2001) A bacterial method for the nitrogen isotopic analysis of nitrate in seawater and freshwater. Anal Chem 73:4145-4153</p>
<p>Weigand MA, Foriel J, Barnett B, Oleynik S, Sigman DM (in review) Updates to instrumentation and protocols for isotopic analysis of nitrate by the denitrifier method. Rapid Commun Mass Spectrom</p>
Specified by the Principal Investigator(s)
<p>Standard deviations are derived from at least two analytical measurements</p>
<p><strong>BCO-DMO Processing:</strong></p>
<p>-&nbsp;added conventional header with dataset name, PI name, version date<br />
- renamed parameters to BCO-DMO standard<br />
- replaced blank cells with nd<br />
- formatted lat and long to 4 decimal places</p>
<p>- replaced&nbsp;original 2016-07-13 EN532 data with new version submitted 2017-07-14. Cast numbers were rearranged.</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
PI Supplied Instrument Name: Instrument Name: CTD Sea-Bird SBE 911plus Instrument Short Name:CTD SBE 911plus Instrument Description: The Sea-Bird SBE 911 plus is a type of CTD instrument package for continuous measurement of conductivity, temperature and pressure. The SBE 911 plus includes the SBE 9plus Underwater Unit and the SBE 11plus Deck Unit (for real-time readout using conductive wire) for deployment from a vessel. The combination of the SBE 9 plus and SBE 11 plus is called a SBE 911 plus. The SBE 9 plus uses Sea-Bird's standard modular temperature and conductivity sensors (SBE 3 plus and SBE 4). The SBE 9 plus CTD can be configured with up to eight auxiliary sensors to measure other parameters including dissolved oxygen, pH, turbidity, fluorescence, light (PAR), light transmission, etc.). more information from Sea-Bird Electronics Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0058/
GC-IRMS
GC-IRMS
PI Supplied Instrument Name: GC-IRMS PI Supplied Instrument Description:Thermo MAT 253 mass spectrometer Instrument Name: Isotope-ratio Mass Spectrometer Instrument Short Name:IR Mass Spec; IRMS Instrument Description: The Isotope-ratio Mass Spectrometer is a particular type of mass spectrometer used to measure the relative abundance of isotopes in a given sample (e.g. VG Prism II Isotope Ratio Mass-Spectrometer). Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB16/
PI Supplied Instrument Name: Instrument Name: Niskin bottle Instrument Short Name:Niskin bottle Instrument Description: A Niskin bottle (a next generation water sampler based on the Nansen bottle) is a cylindrical, non-metallic water collection device with stoppers at both ends. The bottles can be attached individually on a hydrowire or deployed in 12, 24, or 36 bottle Rosette systems mounted on a frame and combined with a CTD. Niskin bottles are used to collect discrete water samples for a range of measurements including pigments, nutrients, plankton, etc. Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0412/
Cruise: EN532
EN532
R/V Endeavor
Community Standard Description
International Council for the Exploration of the Sea
R/V Endeavor
vessel
EN532
Bess B. Ward
Princeton University
http://dmoserv3.bco-dmo.org/data_docs/DimBio_NABE/EN532_CruiseReport.pdf
Report describing EN532
R/V Endeavor
Community Standard Description
International Council for the Exploration of the Sea
R/V Endeavor
vessel