<div><p>All samples were collected via Niskin bottles.</p>
<p>Seawater samples were fixed with 2% borate-buffered formaldehyde, frozen overnight, then filtered the next day onto 0.2-um polycarbonate membrane filters. Filters were refrozen at -20 degrees celsius until hybridization. All FISH hybridization procedures were performed according to Pernthaler et al. (2001) with fluorescent probes labeled with CY3 or fluorescein. Filters were counterstained with DAPI and probe-positive cells were enumerated as a percentage of the total community (DAPI-positive). FISH probes were:</p>
<p><img alt="" src="https://datadocs.bco-dmo.org/d3/data_docs/CariacoMetaOmics/FISH_table.png" style="height:105px; width:739px" /></p></div>
Fluorescent in situ hybridization data from the CAR212 and CAR216 cruises.
<div><p>Fluorescent in situ hybridization (FISH) data enumerated from microscopic samples on 2 legs of each of the CAR212 and CAR216 cruises.</p></div>
Fluorescent in situ hybridization data from the Cariaco Basin
<div><p>All data were normalized to volume of seawater.</p>
<p><span style="font-size:11px">DMO Notes:</span></p>
<p><span style="font-size:11px">-transferred FISH data section into separate a data object.<br />
-reformatted the date to comply with BCO-DMO standards.<br />
-reformatted some column names to comply with BCO-DMO standards.</span></p></div>
652438
Fluorescent in situ hybridization data from the Cariaco Basin
2016-07-22T13:40:42-04:00
2016-07-22T13:40:42-04:00
2023-07-07T16:10:26-04:00
urn:bcodmo:dataset:652438
Fluorescent in situ hybridization data from B/O Hermano Gines cruises for the CARIACO Basin Time Series Station from May to November 2014 (CariacoMetaOmics project)
false
Taylor, G. T., Edgcomb, V. P., Taylor, C. (2016) Fluorescent in situ hybridization data from B/O Hermano Gines cruises for the CARIACO Basin Time Series Station from May to November 2014 (CariacoMetaOmics project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version Final) Version Date 2016-07-22 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/652438 [access date]
true
Final
false
2016-07-22
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Final with updates expected