http://lod.bco-dmo.org/id/dataset/653431
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2016-08-08
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Particulate B vitamins from Hotmix expedition, B/O Sarmiento de Gamboa Hotmix, May 2014
2016-08-08
publication
2016-08-08
revision
BCO-DMO Linked Data URI
2016-08-08
creation
http://lod.bco-dmo.org/id/dataset/653431
Sergio A. Sanudo-Wilhelmy
University of Southern California
principalInvestigator
Feixue Fu
University of Southern California
principalInvestigator
David A. Hutchins
University of Southern California
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Sanudo-Wilhelmy, S. A., Hutchins, D. A., Fu, F. (2016) Particulate B vitamins from Hotmix expedition, B/O Sarmiento de Gamboa Hotmix, May 2014. Biological and Chemical Oceanography Data Management Office (BCO-DMO). Version Date 2016-08-08 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/653431 [access date]
Particulate B vitamins from Hotmix expedition, 2014 Dataset Description: <p>Access to this data set is restricted until 2017-12-31. Please contact L. Cutter for further information.</p>
<p><strong>Related Datasets:</strong><br />
<a href="http://www.bco-dmo.org/dataset/653404">vitamin B dissolved - Hotmix 2014</a><br />
<a href="http://www.bco-dmo.org/dataset/653453">vitamin B particulate - Vitacopss 2015</a></p> Methods and Sampling: <p>Samples for quantification of dissolved and particulate B vitamins were collected at a number of depths within the euphotic zone (see field study sites). Seawater was collected from each CTD depth using Niskin bottles and immediately filtered. Particulate samples were collected using 0.2µm in-line filters and a peristaltic pump (flow rate &lt; 50 ml per minute), transferred into sterile cryovials. and were immediately stored at -80 degrees C until analysis. One liter of filtrate was collected and stored in 1-L bottles at -20 degrees C until analysis. Dissolved B-vitamin pre-concentration was performed as described in Suffridge et. al (2017) and Sañudo et al 2012.</p>
<p>All vitamin samples were analyzed by liquid chromatography/tandem mass spectrometry (LC/MS/MS). The LCMS system consists of a ThermoTSQ Quantum Access electro-spray ionization triple quadrupole mass spectrometer, coupled to a Thermo Accela High Speed Liquid Chromatography system.</p>
<p>Particulate B-vitamins were extracted in acidic methanol (pH 3.5, 5% MeOH, 95% LC/MS Grade water) using bead-beating for cell lysis and a 30C incubation for B-vitamin extraction.&nbsp; A liquid phase extraction using chloroform (1:1 v/v) was used to clean up the samples prior to analysis. The extraction was performed as described in Suffridge et. al (submitted to L&amp;O Methods, June 2016).&nbsp;</p>
<p>Cited References:</p>
<p>Suffridge, Christopher; Cutter, Lynda; Sanudo-Wilhelmy, Sergio (2017) A New Analytical Method for Direct Measurement of Particulate and Dissolved B-vitamins and Their Congeners in Seawater. Front. Mar. Sci. vol.4 doi.org/10.3389/fmars.2017.00011</p>
<p>Sanudo 2012 -&nbsp;Proc Natl Acad Sci U S A. 2012 Aug 28;109(35):14041-5. doi: 10.1073/pnas.1208755109. Epub 2012 Jul 23</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1435666 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1435666
completed
Sergio A. Sanudo-Wilhelmy
University of Southern California
213-821-1302
3616 Trousdale Pkwy Dept. of Biological Sciences, AHF 206, Mail Code: 0371
Los Angeles
CA
90089
USA
sanudo@usc.edu
pointOfContact
Feixue Fu
University of Southern California
2137403188
Department of Biology, MC 0371, University of Southern California 3616 Trousdale Pkwy
Los Angeles
CA
90089
USA
ffu@usc.edu
pointOfContact
David A. Hutchins
University of Southern California
213-740-5616
Department of Biological Sciences 3616 Trousdale Parkway, AHF 20
Los Angeles
CA
90089
USA
dahutch@usc.edu
pointOfContact
asNeeded
Unknown
station
lat
lon
depth
analyte
mean_pM
std_dev
theme
None, User defined
station
latitude
longitude
depth
sample description
No BCO-DMO term
standard deviation
featureType
BCO-DMO Standard Parameters
CTD - profiler
High-Performance Liquid Chromatograph
Mass Spectrometer
Niskin bottle
instrument
BCO-DMO Standard Instruments
Hotmix2014
service
Deployment Activity
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Can the availability of B-vitamins control phyto-and-bacterioplankton successions in a coastal upwelling region?
https://osprey.bco-dmo.org/project/516960
Can the availability of B-vitamins control phyto-and-bacterioplankton successions in a coastal upwelling region?
<p><em>Description from NSF award abstract:</em><br />
B-vitamins (thiamin (B1), biotin (B7), and cobalamin (B12)) are organic molecules used by all organisms for many biochemical reactions ranging from DNA and amino acid synthesis to carbon dioxide assimilation. Despite their metabolic importance, many marine organisms cannot make them and need to obtain them from the environment. Because the requirement for a specific vitamin is different for different organisms, changes in the species composition of algae could be explained by their different B-vitamin requirements. For example, changes in the biological properties of waters during an algal bloom (removal of needed vitamins and release of other vitamins) may favor algae that require the vitamin released by the previous bloom (setting up a floral succession). This selective preconditioning of the waters may be one factor in the seasonal succession of algal species. However, evaluating the role of vitamins in marine ecology has been difficult. No study to date has been comprehensive enough to estimate the importance of vitamins in primary productivity and species succession. This is especially true in coastal upwelling regions that although relatively small in area, are orders of magnitude more productive than their open-ocean counterparts. In fact, those regions contribute a significant portion of the world fisheries. Therefore, in order to try to predict future changes in the world ocean due to human activity, the variables that influence or control the algal communities that dominate the very productive food chains of upwelling regions need to be identified.</p>
<p>This study will investigate how the availability of B-vitamins affects the dynamics of algal- and bacterioplankton population growth in coastal waters of an upwelling region off Southern California. This comprehensive field investigation will determine in situ temporal concentrations of several dissolved and particulate B-vitamins, inorganic micro- and macronutrients, concurrently with seasonal changes in phytoplankton and bacterial abundances and species composition at a long-term time series station within the San Pedro Basin near Los Angeles. Those measurements will be complemented with field incubation experiments with natural plankton assemblages to study the effect of organic and inorganic nutrient amendments on phytoplankton and bacterial community structure. This study will establish for the first time that the availability of ambient B-vitamins influence algal and bacterial species succession in a highly productive coastal upwelling region and that multiple and differing B-vitamin requirements limit growth of some phytoplankton species in those areas. Furthermore, this study will try to show that coastal upwelling transports some B-vitamins to the phytoplankton community in the photic zone from bacterially-influenced source waters within the upper mesopelagic zone.</p>
B-vitamin plankton succession
largerWorkCitation
project
eng; USA
oceans
2016-08-08
Southern California Bight
0
BCO-DMO catalogue of parameters from Particulate B vitamins from Hotmix expedition, B/O Sarmiento de Gamboa Hotmix, May 2014
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/653445.rdf
Name: station
Units: unitless
Description: <p>station number</p>
http://lod.bco-dmo.org/id/dataset-parameter/653446.rdf
Name: lat
Units: decimal degrees
Description: <p>latitude; north is positive</p>
http://lod.bco-dmo.org/id/dataset-parameter/653447.rdf
Name: lon
Units: decimal degrees
Description: <p>longitude; east is positive</p>
http://lod.bco-dmo.org/id/dataset-parameter/653448.rdf
Name: depth
Units: meters
Description: <p>sample depth</p>
http://lod.bco-dmo.org/id/dataset-parameter/653449.rdf
Name: analyte
Units: unitless
Description: <p>dissolved vitamin-B form:<br />
AB12: Adenosyl Cobalamin<br />
B1: Thiamine<br />
B7: Biotin<br />
CB12: Cyanocobalamin<br />
HB12: Hydroxycobalamin<br />
HMP: 4-Amino-2-methyl-5-pyrimidinyl methanol<br />
MB12: Methylcobalamin<br />
MET: Methionine<br />
TMP: Thiamine monophosphate<br />
TPP: Thiamine pyrophosphate</p>
http://lod.bco-dmo.org/id/dataset-parameter/653450.rdf
Name: mean_pM
Units: picomoles per liter
Description: <p>analyte concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/653451.rdf
Name: std_dev
Units: picomoles per liter
Description: <p>concentration standard deviation</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
https://osprey.bco-dmo.org/dataset/653431/data/download
download
onLine
dataset
<p>Samples for quantification of dissolved and particulate B vitamins were collected at a number of depths within the euphotic zone (see field study sites). Seawater was collected from each CTD depth using Niskin bottles and immediately filtered. Particulate samples were collected using 0.2µm in-line filters and a peristaltic pump (flow rate &lt; 50 ml per minute), transferred into sterile cryovials. and were immediately stored at -80 degrees C until analysis. One liter of filtrate was collected and stored in 1-L bottles at -20 degrees C until analysis. Dissolved B-vitamin pre-concentration was performed as described in Suffridge et. al (2017) and Sañudo et al 2012.</p>
<p>All vitamin samples were analyzed by liquid chromatography/tandem mass spectrometry (LC/MS/MS). The LCMS system consists of a ThermoTSQ Quantum Access electro-spray ionization triple quadrupole mass spectrometer, coupled to a Thermo Accela High Speed Liquid Chromatography system.</p>
<p>Particulate B-vitamins were extracted in acidic methanol (pH 3.5, 5% MeOH, 95% LC/MS Grade water) using bead-beating for cell lysis and a 30C incubation for B-vitamin extraction.&nbsp; A liquid phase extraction using chloroform (1:1 v/v) was used to clean up the samples prior to analysis. The extraction was performed as described in Suffridge et. al (submitted to L&amp;O Methods, June 2016).&nbsp;</p>
<p>Cited References:</p>
<p>Suffridge, Christopher; Cutter, Lynda; Sanudo-Wilhelmy, Sergio (2017) A New Analytical Method for Direct Measurement of Particulate and Dissolved B-vitamins and Their Congeners in Seawater. Front. Mar. Sci. vol.4 doi.org/10.3389/fmars.2017.00011</p>
<p>Sanudo 2012 -&nbsp;Proc Natl Acad Sci U S A. 2012 Aug 28;109(35):14041-5. doi: 10.1073/pnas.1208755109. Epub 2012 Jul 23</p>
Specified by the Principal Investigator(s)
<p><strong>BCO-DMO Processing:</strong></p>
<p>- added conventional header with dataset name, PI name, version date<br />
- renamed parameters to BCO-DMO standard<br />
- replaced blank cells with nd (no data)<br />
- mean_pM and std_dev: reduced digits right of decimal from 9 to 1 to take measurement precision into consideration</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
PI Supplied Instrument Name: Instrument Name: CTD - profiler Instrument Short Name: Instrument Description: The Conductivity, Temperature, Depth (CTD) unit is an integrated instrument package designed to measure the conductivity, temperature, and pressure (depth) of the water column. The instrument is lowered via cable through the water column. It permits scientists to observe the physical properties in real-time via a conducting cable, which is typically connected to a CTD to a deck unit and computer on a ship. The CTD is often configured with additional optional sensors including fluorometers, transmissometers and/or radiometers. It is often combined with a Rosette of water sampling bottles (e.g. Niskin, GO-FLO) for collecting discrete water samples during the cast.
This term applies to profiling CTDs. For fixed CTDs, see https://www.bco-dmo.org/instrument/869934. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/130/
PI Supplied Instrument Name: PI Supplied Instrument Description:Thermo Accela High Speed Liquid Chromatography system Instrument Name: High-Performance Liquid Chromatograph Instrument Short Name:HPLC Instrument Description: A High-performance liquid chromatograph (HPLC) is a type of liquid chromatography used to separate compounds that are dissolved in solution. HPLC instruments consist of a reservoir of the mobile phase, a pump, an injector, a separation column, and a detector. Compounds are separated by high pressure pumping of the sample mixture onto a column packed with microspheres coated with the stationary phase. The different components in the mixture pass through the column at different rates due to differences in their partitioning behavior between the mobile liquid phase and the stationary phase. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB11/
PI Supplied Instrument Name: PI Supplied Instrument Description:ThermoTSQ Quantum Access electro-spray ionization triple quadrupole mass spectrometer Instrument Name: Mass Spectrometer Instrument Short Name:Mass Spec Instrument Description: General term for instruments used to measure the mass-to-charge ratio of ions; generally used to find the composition of a sample by generating a mass spectrum representing the masses of sample components. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB16/
PI Supplied Instrument Name: Instrument Name: Niskin bottle Instrument Short Name:Niskin bottle Instrument Description: A Niskin bottle (a next generation water sampler based on the Nansen bottle) is a cylindrical, non-metallic water collection device with stoppers at both ends. The bottles can be attached individually on a hydrowire or deployed in 12, 24, or 36 bottle Rosette systems mounted on a frame and combined with a CTD. Niskin bottles are used to collect discrete water samples for a range of measurements including pigments, nutrients, plankton, etc. Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0412/
Cruise: Hotmix2014
Hotmix2014
B/O Sarmiento de Gamboa
Community Standard Description
International Council for the Exploration of the Sea
B/O Sarmiento de Gamboa
vessel
B/O Sarmiento de Gamboa
Community Standard Description
International Council for the Exploration of the Sea
B/O Sarmiento de Gamboa
vessel