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        <gco:CharacterString>Physical data collected by at towed plankton imaging system in a subtropical, pelagic environment Dataset Description: &amp;lt;p&amp;gt;Physical data collected by at towed plankton imaging system in a subtropical, pelagic environment; collected on&amp;amp;nbsp;R/V Walton Smith cruises, WS1406 and&amp;amp;nbsp;WS15161.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;Status note:&amp;amp;nbsp;&amp;lt;/strong&amp;gt;These data are restricted from public access until the related manuscript has been published.&amp;lt;/p&amp;gt; Methods and Sampling: &amp;lt;p&amp;gt;The &amp;lt;em&amp;gt;In Situ&amp;lt;/em&amp;gt; Ichthyoplankton Imaging System (ISIIS) is a high resolution imaging system designed to image sufficient volumes of water to accurately quantify rare meso- and macroplankton such as fish and crab larvae, and also associated plankters (e.g., ctenophores, larvaceans, euphausiids, chaetognaths, hydrozoans, scyphozoans, pteropods, copepods) &amp;lt;em&amp;gt;in situ&amp;lt;/em&amp;gt; (Cowen &amp;amp;amp; Guigand 2008). An additional, finer scale imaging system has been added to the current system (ISIIS-2) and is capable of imaging up to 140 L per second, with pixel resolution of 45-68 um. This yields particle resolution down to ~ 450 um, adding considerable breadth to the full size range of organisms that can be detected and enumerated. The ISIIS is also instrumented with a set of environmental sensors including: Seabird CTD (SBE 49), Biospherical PAR (QCP2300), Seabird dissolved O&amp;lt;sub&amp;gt;2 &amp;lt;/sub&amp;gt;(SBE 43), and Wet Labs Fluorometer (ECO). All data are passed via fiber optic cable to an onboard computer, time-stamped for cross-referencing, and saved on a high-speed disk array. Oxygen data from 2014 were excluded as they were found to be outside the calibrated range of the sensor.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;Related references:&amp;lt;/strong&amp;gt;&amp;lt;br /&amp;gt;
Cowen RK, Guigand CM. 2008. In situ Ichthyoplankton Imaging System (ISIIS): system design and preliminary results. Limnol Oceanogr Methods. 6: 126-132. doi:&amp;lt;a href=&amp;quot;http://dx.doi.org/10.4319/lom.2008.6.126&amp;quot; target=&amp;quot;_blank&amp;quot;&amp;gt;10.4319/lom.2008.6.126&amp;lt;/a&amp;gt;&amp;lt;/p&amp;gt;</gco:CharacterString>
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        <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/award/529695.rdf" xlink:title="OCE-1419987" xlink:actuate="onRequest">Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1419987 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1419987</gmx:Anchor>
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            http://lod.bco-dmo.org/id/dataset-parameter/655572.rdf
	Name: cruise_name
	Units: unitless
	Description: &lt;p&gt;Cruise name&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/655573.rdf
	Name: cruise_id
	Units: unitless
	Description: &lt;p&gt;Official cruise identifier&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/655574.rdf
	Name: transect
	Units: unitless
	Description: &lt;p&gt;Unique transect identifier&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/655575.rdf
	Name: depth
	Units: meters (m)
	Description: &lt;p&gt;Depth&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/655576.rdf
	Name: ISO_DateTime_Local
	Units: unitless
	Description: &lt;p&gt;Local date and time (EDT) of observation (24h) formatted to ISO8601 standard&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/655577.rdf
	Name: lat
	Units: decimal degrees North
	Description: &lt;p&gt;Vehicle position latitude&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/655578.rdf
	Name: lon
	Units: decimal degrees West
	Description: &lt;p&gt;Vehicle position longitude&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/655579.rdf
	Name: temp
	Units: degrees Celsius
	Description: &lt;p&gt;Water temperature&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/655580.rdf
	Name: sal
	Units: parts per thousand
	Description: &lt;p&gt;Salinity&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/655581.rdf
	Name: press
	Units: decibars (db)
	Description: &lt;p&gt;Pressure&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/655582.rdf
	Name: density
	Units: kilograms per cubic meter (kg/m3)
	Description: &lt;p&gt;Seawater density&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/655583.rdf
	Name: fluor_v
	Units: volts
	Description: &lt;p&gt;Fluorescence&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/655584.rdf
	Name: chl
	Units: micrograms per Liter (ug/L)
	Description: &lt;p&gt;Derived chlorophyll from fluorescence&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/655585.rdf
	Name: irradiance
	Units: microEinsteins per square cm (uE/cm2)
	Description: &lt;p&gt;Irradiance&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/655586.rdf
	Name: heading
	Units: degrees
	Description: &lt;p&gt;Vehicle heading&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/655587.rdf
	Name: hz_vel_mm_s
	Units: millimeters per second (mm/s)
	Description: &lt;p&gt;Vehicle horizontal velocity&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/655588.rdf
	Name: hz_vel_cm_s
	Units: centimeters per second (cm/s)
	Description: &lt;p&gt;Vehicle horizontal velocity&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/655589.rdf
	Name: vert_vel
	Units: millimeters per second (mm/s)
	Description: &lt;p&gt;Vehicle vertical velocity&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/655590.rdf
	Name: pitch
	Units: degrees
	Description: &lt;p&gt;Vehicle pitch&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/655591.rdf
	Name: lg_hz_line
	Units: centimeters per line (cm/line)
	Description: &lt;p&gt;Large camera horizontal line&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/655592.rdf
	Name: lg_eff_image_len
	Units: centimeters (cm)
	Description: &lt;p&gt;Large camera effective image length&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/655593.rdf
	Name: lg_images_sec
	Units: images/s
	Description: &lt;p&gt;Large camera images per second&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/655594.rdf
	Name: lg_vol_imaged
	Units: cubic centimeters (cm3)
	Description: &lt;p&gt;Large camera volume imaged&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/655595.rdf
	Name: lg_vol_rate
	Units: cubic meters per second (m3/s)
	Description: &lt;p&gt;Large camera volume rate&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/655596.rdf
	Name: sm_hz_line
	Units: centimeters per line (cm/line)
	Description: &lt;p&gt;Small camera horizontal line&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/655597.rdf
	Name: sm_eff_image_len
	Units: centimeters (cm)
	Description: &lt;p&gt;Small camera effective image length&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/655598.rdf
	Name: sm_images_sec
	Units: images/s
	Description: &lt;p&gt;Small camera images per second&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/655599.rdf
	Name: sm_vol_imaged
	Units: cubic centimeters (cm3)
	Description: &lt;p&gt;Small camera imaged volume&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/655600.rdf
	Name: sm_vol_rate
	Units: cubic meters per second (m3/s)
	Description: &lt;p&gt;Small camera volume rate&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/655601.rdf
	Name: oxygen
	Units: milliliters per liter (mL/L)
	Description: &lt;p&gt;Dissolved oxygen&lt;/p&gt; 
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                <gco:CharacterString>&amp;lt;p&amp;gt;The &amp;lt;em&amp;gt;In Situ&amp;lt;/em&amp;gt; Ichthyoplankton Imaging System (ISIIS) is a high resolution imaging system designed to image sufficient volumes of water to accurately quantify rare meso- and macroplankton such as fish and crab larvae, and also associated plankters (e.g., ctenophores, larvaceans, euphausiids, chaetognaths, hydrozoans, scyphozoans, pteropods, copepods) &amp;lt;em&amp;gt;in situ&amp;lt;/em&amp;gt; (Cowen &amp;amp;amp; Guigand 2008). An additional, finer scale imaging system has been added to the current system (ISIIS-2) and is capable of imaging up to 140 L per second, with pixel resolution of 45-68 um. This yields particle resolution down to ~ 450 um, adding considerable breadth to the full size range of organisms that can be detected and enumerated. The ISIIS is also instrumented with a set of environmental sensors including: Seabird CTD (SBE 49), Biospherical PAR (QCP2300), Seabird dissolved O&amp;lt;sub&amp;gt;2 &amp;lt;/sub&amp;gt;(SBE 43), and Wet Labs Fluorometer (ECO). All data are passed via fiber optic cable to an onboard computer, time-stamped for cross-referencing, and saved on a high-speed disk array. Oxygen data from 2014 were excluded as they were found to be outside the calibrated range of the sensor.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;Related references:&amp;lt;/strong&amp;gt;&amp;lt;br /&amp;gt;
Cowen RK, Guigand CM. 2008. In situ Ichthyoplankton Imaging System (ISIIS): system design and preliminary results. Limnol Oceanogr Methods. 6: 126-132. doi:&amp;lt;a href=&amp;quot;http://dx.doi.org/10.4319/lom.2008.6.126&amp;quot; target=&amp;quot;_blank&amp;quot;&amp;gt;10.4319/lom.2008.6.126&amp;lt;/a&amp;gt;&amp;lt;/p&amp;gt;</gco:CharacterString>
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                <gco:CharacterString>&amp;lt;p&amp;gt;Temperature, salinity, pressure, depth, oxygen, fluorescence, and irradiance data are collected at a higher temporal frequency than latitude and longitude observations that come from the ship’s NEMA system. Latitude and longitude values have been linearly interpolated between observations using time.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;In 2014, latitude and longitude data from the NEMA system were not automatically logged with the physical data during ISIIS tows. To address this, temperature, salinity, pressure, depth, oxygen, fluorescence, and irradiance data were binned to the nearest second (mean for each second bin) in order to merge them with the ship’s GPS data.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Seawater density (kg per cubic&amp;amp;nbsp;meter)&amp;amp;nbsp;was calculated from pressure, temperature, and salinity using the UNESCO equation of state from the ‘R’ Library ‘OCE’ package.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Temperature, salinity, oxygen, fluorescence, irradiance values less than zero were excluded as they are erroneous&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Density values less than 1000 kg per cubic meter&amp;amp;nbsp;were excluded as they are erroneous&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Oxygen (2015 only) was corrected for post-calibration sensor drift using the equation:&amp;lt;br /&amp;gt;
Final oxygen (ml L&amp;lt;sup&amp;gt;-1&amp;lt;/sup&amp;gt;) = Initial oxygen (ml L&amp;lt;sup&amp;gt;-1&amp;lt;/sup&amp;gt;)*1.0649702 – 0.0029434&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Fluorescence (volts) data from the Wet Labs Eco Fluorometer was converted to chlorophyll (ug/L) using the following equation:&amp;lt;br /&amp;gt;
chlorophyll (µg L&amp;lt;sup&amp;gt;-1&amp;lt;/sup&amp;gt;) = scale factor*(volts – dark counts)&amp;lt;br /&amp;gt;
2014: Scale factor = 25; Dark counts = 0.028. 2015: Scale factor = 24; Dark counts = 0.026.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;Large camera parameters used in volume imaged calculations:&amp;lt;/strong&amp;gt;&amp;lt;br /&amp;gt;
&amp;lt;em&amp;gt;&amp;lt;strong&amp;gt;2014:&amp;lt;/strong&amp;gt;&amp;lt;/em&amp;gt;&amp;lt;br /&amp;gt;
Image width: 2048 pixels&amp;lt;br /&amp;gt;
Scan rate: 35,000 lines s-1&amp;lt;br /&amp;gt;
Depth of field: 50 cm&amp;lt;br /&amp;gt;
Field of view: 11.2 cm&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;&amp;lt;em&amp;gt;&amp;lt;strong&amp;gt;2015:&amp;lt;/strong&amp;gt;&amp;lt;/em&amp;gt;&amp;lt;br /&amp;gt;
Image width: 2048 pixels&amp;lt;br /&amp;gt;
Scan rate: 35,000 lines s-1&amp;lt;br /&amp;gt;
Depth of field: 50 cm&amp;lt;br /&amp;gt;
Field of view: 11 cm&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;Small camera parameters used in volume imaged calculations:&amp;lt;/strong&amp;gt;&amp;lt;br /&amp;gt;
&amp;lt;em&amp;gt;&amp;lt;strong&amp;gt;2014:&amp;lt;/strong&amp;gt;&amp;lt;/em&amp;gt;&amp;lt;br /&amp;gt;
Image width: 1024 pixels&amp;lt;br /&amp;gt;
Scan rate: 62,000 lines s-1&amp;lt;br /&amp;gt;
Depth of field: 10 cm&amp;lt;br /&amp;gt;
Field of view: 6 cm&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;&amp;lt;em&amp;gt;&amp;lt;strong&amp;gt;2015:&amp;lt;/strong&amp;gt;&amp;lt;/em&amp;gt;&amp;lt;br /&amp;gt;
Image width: 1024 pixels&amp;lt;br /&amp;gt;
Scan rate: 55,000 lines s-1&amp;lt;br /&amp;gt;
Depth of field: 10 cm&amp;lt;br /&amp;gt;
Field of view: 4.6 cm&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;Derived camera data calculated using equations below:&amp;lt;/strong&amp;gt;&amp;lt;br /&amp;gt;
lg.hz.line = &amp;amp;nbsp;horizontal.velocity (cm s-1) / lg.scan.rate&amp;lt;br /&amp;gt;
lg.eff.image.length (cm) = lg.image.width *lg.hz.line&amp;lt;br /&amp;gt;
lg.vol.image (cm3) = lg.dof*lg.fov*lg.eff.image.length&amp;lt;br /&amp;gt;
lg.image.sec (images s-1) = horizontal.velocity (cm s-1)/lg.eff.image.length&amp;lt;br /&amp;gt;
lg.vol.rate (m3 s-1) = (lg.image.sec*lg.vol.image)*1E-06&amp;lt;br /&amp;gt;
sm.hz.line = &amp;amp;nbsp;horizontal.velocity (cm s-1) / sm.scan.rate&amp;lt;br /&amp;gt;
sm.eff.image.length (cm) = sm.image.width *sm.hz.line&amp;lt;br /&amp;gt;
sm.vol.image (cm3) = sm.dof*sm.fov*sm.eff.image.length&amp;lt;br /&amp;gt;
sm.image.sec (images s-1) = horizontal.velocity (cm s-1)/sm.eff.image.length&amp;lt;br /&amp;gt;
sm.vol.rate (m3 s-1) = (sm.image.sec*sm.vol.image)*1E-06&amp;lt;/p&amp;gt;</gco:CharacterString>
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            <gco:CharacterString>PI Supplied Instrument Name: ISIIS PI Supplied Instrument Description:The In Situ Ichthyoplankton Imaging System (ISIIS) is a high resolution imaging system designed to image sufficient volumes of water to accurately quantify rare meso- and macroplankton such as fish and crab larvae, and also associated plankters (e.g., ctenophores, larvaceans, euphausiids, chaetognaths, hydrozoans, scyphozoans, pteropods, copepods) in situ (Cowen &amp; Guigand 2008). An additional, finer scale imaging system has been added to the current system (ISIIS-2) and is capable of imaging up to 140 L s-1, with pixel resolution of 45-68 µm. This yields particle resolution down to ~ 450 µm, adding considerable breadth to the full size range of organisms that can be detected and enumerated. The ISIIS is also instrumented with a set of environmental sensors including: Seabird CTD (SBE 49), Biospherical PAR (QCP2300), Seabird dissolved O2 (SBE 43), and Wet Labs Fluorometer (ECO). All data are passed via fiber optic cable to an onboard computer, time-stamped for cross-referencing, and saved on a high-speed disk array. Oxygen data from 2014 were excluded as they were found to be outside the calibrated range of the sensor. Instrument Name: In Situ Ichtyoplankton Imaging System Instrument Short Name:ISIIS   Instrument Description: The In Situ Ichthyoplankton Imaging System (ISIIS) is an underwater imaging system aimed at capturing in situ, real time images of marine zooplankton of relatively low abundance such as fish larvae and fragile gelatinous organisms. The first prototype, delivered in 2007, was attached to a relatively simple vehicle towed by an oceanographic vessel at a speed of five knots. The vehicle, and associated imaging system and sensors, was moved up and down through the water column by paying cable in and out via an oceanographic winch. Subsequently, a new vehicle has been designed with the capacity of self undulation using motor actuated dive fins. 

The ISIIS system utilizes a high-resolution line-scanning camera with a Light Emitting Diode (LED) light source, modified by plano-convex optics, to create a collimated light field to back-light a parcel of water.

ISIIS was developed in collaboration between the University of Miami's Rosenstiel School of Atmospheric and Marine Science (RSMAS) and the subsea engineering company, Bellamare, LLC, located in San Diego CA. See complete description from RSMAS.

Reference:
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