http://lod.bco-dmo.org/id/dataset/658894
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2016-09-16
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Juvenile growth post-settlement under four larval-to-juvenile ocean acidification transitions in the laboratory; conducted at Bodega Marine Lab in July-Sept 2009
2016-09-16
publication
2016-09-16
revision
BCO-DMO Linked Data URI
2016-09-16
creation
http://lod.bco-dmo.org/id/dataset/658894
Brian Gaylord
University of California - Davis: Bodega Marine Laboratory
principalInvestigator
Tessa M. Hill
University of California - Davis: Bodega Marine Laboratory
principalInvestigator
Ann D. Russell
University of California-Davis
principalInvestigator
Eric Sanford
University of California - Davis: Bodega Marine Laboratory
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Gaylord, B., Hill, T. M., Sanford, E., Russell, A. D. (2016) Juvenile growth post-settlement under four larval-to-juvenile ocean acidification transitions in the laboratory; conducted at Bodega Marine Lab in July-Sept 2009. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 16 Sept 2016) Version Date 2016-09-16 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/658894 [access date]
Juvenile growth (on day 7 post-settlement) under four larval-to-juvenile ocean acidification transitions in the laboratory Dataset Description: <p>Juvenile growth (on day 7 post-settlement) of Olympia oysters under four larval-to-juvenile ocean acidification transitions in the laboratory.&nbsp;The study examined the consequences of decreased pH and carbonate saturation on the early life stages of Olympia oysters. Oysters were reared in three levels of seawater pH (8.0, 7.9, 7.8) and their growth rates were measured/compared.</p>
<p>Related datasets:<br />
<a href="http://www.bco-dmo.org/dataset/658678" target="_blank">juvenile growth - experiment 1</a><br />
<a href="http://www.bco-dmo.org/dataset/658918" target="_blank">larval growth - experiment 1</a></p> Methods and Sampling: <p><strong>Detailed methodology and results are described in following publication:</strong><br />
Hettinger, A., E. Sanford, T.M. Hill, A.D. Russell, K.N. Sato, J. Hoey, M. Forsch, H.N. Page, and B. Gaylord. 2012. Persistent carry-over effects of planktonic exposure to ocean acidification in the Olympia oyster.&nbsp;Ecology 93: 2758-2768. doi:<a href="http://dx.doi.org/10.1890/12-0567.1" target="_blank">10.1890/12-0567.1</a></p>
<p><strong>Briefly (excerpted from above):</strong><br />
Oysters were reared at Bodega Marine Laboratory (BML) in two experiments. These data are from experiment 1. In both, seawater CO2 concentrations in the treatement cultrues were increased relative to present-day levles by 100 and 400 ppm. Thus, seawater CO2 concentrations employed in the experiments were: 700 (used as an operational control), 800, and 1100 ppm. Such CO2 levels correspond to pH values of approximately 8.0, 7.9, and 7.8 (NBS scale). These nominal pH levels were used subsequently to identify the treatments.&nbsp;</p>
<p>Oysters were reared from early larval life in 4.5-L glass culture jars held in seawater tables maintained at 20.0 (+/- 0.02) degrees C. All seawater used during rearing was filtered at 0.45 um and pre-adjusted to appropriate pH levels in 20 L carboys by bubbling for 2-3 days with NIST-traceable CO2 air mixtures (hereafter referred to as "carboy water"). Acrylic boxes mounted over each seawater table received the same mixed gases and provided a common head space for six jars, minimizing off-gassing during culturing. Levels of pH used for each box, and jar position within a box, were randomly assigned.</p>
<p><em>Larval culturing:</em><br />
Adult Olympia oysters (4-7 cm in length) were collected from Tomales Bay and transported to BML. They were cleaned and distributed among multiple 100-L cylinders containing seawater filtered at 0.45 um and held at 18-22 degrees C. &nbsp;At least one female per cylinder released larvae within 48&nbsp;hours post-collection, enabling acquisition of independent "larval cohorts". Following release, larvae were transferred into culture jars containing 2L of seawater filtered at 0.45 um (day 1 of the experiment). Every other day, 90% of the sewater in each jar was changed and replaced with carboy water, whose pH had stabilized to the appropriate level.</p>
<p><em>Water chemistry:</em><br />
Samples of jar water and carboy water were collected every day. Seawater pH (NBS) and temperature were measured using a pH/temperature meter (Accumet Excel XL60; Thermo Fisher Scientific, Waltham, Massachusetts, USA), and salinity was determined using a YSI 6600V2 multiparameter instrument (YSI, Yellow Springs, Ohio, USA). Alkalinity was measured using automated Gran titration (Metrohm 809; Metrohm, Herisau, Switzerland), and standardized using certified reference material&nbsp;from A. Dickson at Scripps Institution of Oceanography. Other carbonate system parameters were calculated&nbsp;using the software, CO2SYS (Lewis and Wallace 1998).</p>
<p><em>Sampling of larvae and juveniles:</em><br />
Oysters in the culture jars were sampled at key time points during each experiment to quantify shell size and growth rate (change in shell area per day).&nbsp;On day 1, 100 larvae per larval cohort were collected haphazardly by pipette, fixed in 95% ethanol, and individually photographed under a microscope (Leica DM1000 with DC290 camera; Leica Microsystems, Wetzlar, Germany) for analysis using ImageJ software (version 1.37) to determine the initial projected area of the shell (software available online). Larval shell growth rates at later time points were calculated similarly. Juvenile shell growth rate following settlement was determined by measuring the projected shell area of settled individuals from photographs, subtracting the area of the larval shell (which remains visually distinct), and dividing that value by the number of days postsettlement.</p>
<p><em>Experiment 2:</em><br />
Reduced pH induced a negative effect on juvenile growth rate. This outcome (of experiment 1) could have arisen from one of two causes: direct effects of seawater acidification during the early juvenile phase or carry-over effects of larval experience. Experiment 2 (September–October 2009) was conducted to distinguish among these alternatives.</p>
<p>In the first phase of Experiment 2, larvae were pooled across cohorts (300 larvae/cohort; 900 larvae/replicate jar) and reared through settlement under either control or low pH conditions(pH 8.0 or 7.8). The pH levels were randomly assigned to four boxes (2 pH levels x 2 boxes x 3 replicate jars = 12 jars). In the second phase of the experiment, and within 24 h of larval settlement, one-half of the juveniles reared as larvae in control pH conditions were transferred to low pH conditions, and one-half were returned to control pH conditions. Similarly, one-half of the juveniles that had been reared as larvae in low pH conditions were transferred to control pH conditions, and one-half were returned to low pH conditions.</p>
<p>The second phase of the experiment used 2 pH levels x 4 larval-juvenile pH treatments x 6 replicate jars = 24 jars. The juveniles in the two destination pH levels were reared until 13 days after settlement, and randomly selected juveniles on wedges (N=17 per jar) were photographed 7 and 13 days after settlement to quantify shell growth rate since settlement. Larval settlement occurred 24 hours earlier (day 13) under low pH conditions; therefore all photographs taken of settled juveniles in control conditions were delayed by one day.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-0927255 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=0927255
completed
Brian Gaylord
University of California - Davis: Bodega Marine Laboratory
707-875-1940
Bodega Marine Laboratory & Department of Evolution and Ecology 2099 Westshore Road, P.O. Box 247
Bodega Bay
CA
94923
USA
bpgaylord@ucdavis.edu
pointOfContact
Tessa M. Hill
University of California - Davis: Bodega Marine Laboratory
P.O. Box 247 2099 Westshore Road
Bodega Bay
CA
94923
USA
tmhill@ucdavis.edu
pointOfContact
Ann D. Russell
University of California-Davis
Department of Geology One Shields Avenue University of California Davis
Davis
CA
95616
USA
adrussell@ucdavis.edu
pointOfContact
Eric Sanford
University of California - Davis: Bodega Marine Laboratory
P.O. Box 247 2099 Westshore Road
Bodega Bay
CA
94923
USA
edsanford@ucdavis.edu
pointOfContact
asNeeded
Dataset Version: 16 Sept 2016
Unknown
pH_nominal_larval
pH_nominal_juv
avg_size_at_settlement
avg_juv_growth
avg_juv_growth_rate
DC290
Leica DM1000
YSI 6600V2
Accumet Excel XL60
Metrohm 809
Accumet Excel XL60
theme
None, User defined
pH
No BCO-DMO term
growth
featureType
BCO-DMO Standard Parameters
Camera
Microscope - Optical
Multi Parameter Portable Meter
pH Sensor
Titrator
Water Temperature Sensor
instrument
BCO-DMO Standard Instruments
BML_Gaylord
service
Deployment Activity
Bodega Marine Lab, UC Davis
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Bodega Ocean Acidification Research
http://bml.ucdavis.edu/research/research-programs/climate-change/oceanacidification/
Bodega Ocean Acidification Research
<p>The absorption of human-produced CO2 into the world's oceans is decreasing seawater pH and causing marked declines in the saturation state for calcium carbonate, a major building block for shells, skeletons, and tests of many marine species. Such changes (collectively termed "ocean acidification") have the potential to devastate a broad array of organisms, both at the level of individuals and at population and ecosystem scales. Although awareness of these issues is rapidly growing, most of what is known is based on studies of coral reef organisms and plankton.</p>
<p>The proposed work will enhance understanding of impacts from ocean acidification by providing rigorous data on several new fronts applicable to temperate systems. The project will operate within one of the strongest upwelling centers of the eastern Pacific, where global trends in acidification are amplified by the presence of cold water characterized by already-high levels of aqueous CO2. Using an integrated, comparative approach that exploits the expertise of oceanographers, marine chemists, and biologists, the project will explicitly couple moored and shipboard measurements of seawater chemistry to controlled laboratory and field studies of biological responses.</p>
<p>Two vital foundation species (the California mussel, <em>Mytilus californianus</em>, and the Olympia oyster, <em>Ostrea conchaphila</em>) will be targeted. These two species play disproportionately important roles in open-coast and estuarine systems, respectively. Larvae (which are often the most vulnerable stages) of mussels and oysters will be cultured under elevated-CO2 conditions through the full pelagic period and into juvenile life. Growth and survivorship will be quantified, and water temperature and salinity will be varied to test for interactive effects of multiple factors. Intraspecific variation in response of larvae from different parental lineages will be examined. "Carry-over" effects that originate from exposure during the larval stage, but influence subsequent juvenile growth and survival, will be determined both in the laboratory and using field outplants. Because larval and juvenile stages play important roles as demographic age-structure bottlenecks, overall population consequences will be estimated through comparison of observed impacts on early life stages to other recognized sources of recruitment variation.</p>
<p><strong>Data Status:</strong> Data will be reported from the BML offshore oceanographic moorings and from moorings within nearby Tomales Bay. The moorings will be outfitted with autonomously recording pH and pCO2 sensors, and these measurements will be supplemented with discrete water samples collected monthly along two associated transects.</p>
<p><strong>Live Data:</strong> For live-streaming data from Tomales Bay, visit <a href="http://www.ipacoa.org/Explorer" target="_blank">http://www.ipacoa.org/Explorer</a> and click on the icon in Tomales Bay.</p>
BOAR
largerWorkCitation
project
eng; USA
biota
oceans
Bodega Marine Lab, UC Davis
2016-09-16
Central California coast (northeast Pacific)
0
BCO-DMO catalogue of parameters from Juvenile growth post-settlement under four larval-to-juvenile ocean acidification transitions in the laboratory; conducted at Bodega Marine Lab in July-Sept 2009
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/658911.rdf
Name: pH_nominal_larval
Units: pH scale (NBS)
Description: <p>Nominal pH level during larval phase</p>
http://lod.bco-dmo.org/id/dataset-parameter/658912.rdf
Name: pH_nominal_juv
Units: pH scale (NBS)
Description: <p>Nominal pH level during juvenile phase</p>
http://lod.bco-dmo.org/id/dataset-parameter/658913.rdf
Name: avg_size_at_settlement
Units: square millimeters (mm^2)
Description: <p>Average size at settlement</p>
http://lod.bco-dmo.org/id/dataset-parameter/658914.rdf
Name: avg_juv_growth
Units: square millimeters (mm^2)
Description: <p>Average juvenile growth</p>
http://lod.bco-dmo.org/id/dataset-parameter/658915.rdf
Name: avg_juv_growth_rate
Units: square millimeters per day (mm^2/day)
Description: <p>Average juvenile growth rate</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
904
https://datadocs.bco-dmo.org/file/B11vAo1uMZW4pV/juv_growth_exp2.csv
juv_growth_exp2.csv
Primary data file for dataset ID 658894
download
https://osprey.bco-dmo.org/dataset/658894/data/download
download
onLine
dataset
<p><strong>Detailed methodology and results are described in following publication:</strong><br />
Hettinger, A., E. Sanford, T.M. Hill, A.D. Russell, K.N. Sato, J. Hoey, M. Forsch, H.N. Page, and B. Gaylord. 2012. Persistent carry-over effects of planktonic exposure to ocean acidification in the Olympia oyster.&nbsp;Ecology 93: 2758-2768. doi:<a href="http://dx.doi.org/10.1890/12-0567.1" target="_blank">10.1890/12-0567.1</a></p>
<p><strong>Briefly (excerpted from above):</strong><br />
Oysters were reared at Bodega Marine Laboratory (BML) in two experiments. These data are from experiment 1. In both, seawater CO2 concentrations in the treatement cultrues were increased relative to present-day levles by 100 and 400 ppm. Thus, seawater CO2 concentrations employed in the experiments were: 700 (used as an operational control), 800, and 1100 ppm. Such CO2 levels correspond to pH values of approximately 8.0, 7.9, and 7.8 (NBS scale). These nominal pH levels were used subsequently to identify the treatments.&nbsp;</p>
<p>Oysters were reared from early larval life in 4.5-L glass culture jars held in seawater tables maintained at 20.0 (+/- 0.02) degrees C. All seawater used during rearing was filtered at 0.45 um and pre-adjusted to appropriate pH levels in 20 L carboys by bubbling for 2-3 days with NIST-traceable CO2 air mixtures (hereafter referred to as "carboy water"). Acrylic boxes mounted over each seawater table received the same mixed gases and provided a common head space for six jars, minimizing off-gassing during culturing. Levels of pH used for each box, and jar position within a box, were randomly assigned.</p>
<p><em>Larval culturing:</em><br />
Adult Olympia oysters (4-7 cm in length) were collected from Tomales Bay and transported to BML. They were cleaned and distributed among multiple 100-L cylinders containing seawater filtered at 0.45 um and held at 18-22 degrees C. &nbsp;At least one female per cylinder released larvae within 48&nbsp;hours post-collection, enabling acquisition of independent "larval cohorts". Following release, larvae were transferred into culture jars containing 2L of seawater filtered at 0.45 um (day 1 of the experiment). Every other day, 90% of the sewater in each jar was changed and replaced with carboy water, whose pH had stabilized to the appropriate level.</p>
<p><em>Water chemistry:</em><br />
Samples of jar water and carboy water were collected every day. Seawater pH (NBS) and temperature were measured using a pH/temperature meter (Accumet Excel XL60; Thermo Fisher Scientific, Waltham, Massachusetts, USA), and salinity was determined using a YSI 6600V2 multiparameter instrument (YSI, Yellow Springs, Ohio, USA). Alkalinity was measured using automated Gran titration (Metrohm 809; Metrohm, Herisau, Switzerland), and standardized using certified reference material&nbsp;from A. Dickson at Scripps Institution of Oceanography. Other carbonate system parameters were calculated&nbsp;using the software, CO2SYS (Lewis and Wallace 1998).</p>
<p><em>Sampling of larvae and juveniles:</em><br />
Oysters in the culture jars were sampled at key time points during each experiment to quantify shell size and growth rate (change in shell area per day).&nbsp;On day 1, 100 larvae per larval cohort were collected haphazardly by pipette, fixed in 95% ethanol, and individually photographed under a microscope (Leica DM1000 with DC290 camera; Leica Microsystems, Wetzlar, Germany) for analysis using ImageJ software (version 1.37) to determine the initial projected area of the shell (software available online). Larval shell growth rates at later time points were calculated similarly. Juvenile shell growth rate following settlement was determined by measuring the projected shell area of settled individuals from photographs, subtracting the area of the larval shell (which remains visually distinct), and dividing that value by the number of days postsettlement.</p>
<p><em>Experiment 2:</em><br />
Reduced pH induced a negative effect on juvenile growth rate. This outcome (of experiment 1) could have arisen from one of two causes: direct effects of seawater acidification during the early juvenile phase or carry-over effects of larval experience. Experiment 2 (September–October 2009) was conducted to distinguish among these alternatives.</p>
<p>In the first phase of Experiment 2, larvae were pooled across cohorts (300 larvae/cohort; 900 larvae/replicate jar) and reared through settlement under either control or low pH conditions(pH 8.0 or 7.8). The pH levels were randomly assigned to four boxes (2 pH levels x 2 boxes x 3 replicate jars = 12 jars). In the second phase of the experiment, and within 24 h of larval settlement, one-half of the juveniles reared as larvae in control pH conditions were transferred to low pH conditions, and one-half were returned to control pH conditions. Similarly, one-half of the juveniles that had been reared as larvae in low pH conditions were transferred to control pH conditions, and one-half were returned to low pH conditions.</p>
<p>The second phase of the experiment used 2 pH levels x 4 larval-juvenile pH treatments x 6 replicate jars = 24 jars. The juveniles in the two destination pH levels were reared until 13 days after settlement, and randomly selected juveniles on wedges (N=17 per jar) were photographed 7 and 13 days after settlement to quantify shell growth rate since settlement. Larval settlement occurred 24 hours earlier (day 13) under low pH conditions; therefore all photographs taken of settled juveniles in control conditions were delayed by one day.</p>
Specified by the Principal Investigator(s)
<p><strong>BCO-DMO processing:</strong><br />
- modified parameter names to conform with BCO-DMO naming conventions;<br />
- replaced blanks (missing data) with "nd" ("no data").</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
DC290
DC290
PI Supplied Instrument Name: DC290 PI Supplied Instrument Description:Individuals were examined under a microscope (Leica DM1000 with DC290 camera) Instrument Name: Camera Instrument Short Name:camera Instrument Description: All types of photographic equipment including stills, video, film and digital systems. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/311/
Leica DM1000
Leica DM1000
PI Supplied Instrument Name: Leica DM1000 PI Supplied Instrument Description:Individuals were examined under a microscope (Leica DM1000 with DC290 camera) Instrument Name: Microscope - Optical Instrument Short Name: Instrument Description: Instruments that generate enlarged images of samples using the phenomena of reflection and absorption of visible light. Includes conventional and inverted instruments. Also called a "light microscope". Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB05/
YSI 6600V2
YSI 6600V2
PI Supplied Instrument Name: YSI 6600V2 PI Supplied Instrument Description:Salinity was determined usin a YSI 6600V2 multi-parameter instrument (YSI, Yellow Springs, Ohio) Instrument Name: Multi Parameter Portable Meter Instrument Short Name: Instrument Description: An analytical instrument that can measure multiple parameters, such as pH, EC, TDS, DO and temperature with one device and is portable or hand-held.
Accumet Excel XL60
Accumet Excel XL60
PI Supplied Instrument Name: Accumet Excel XL60 PI Supplied Instrument Description:Seawater pH (NBS) and temperature were quantified using a pH/temperature meter: Accumet Excel XL60 (Thermo Fisher Scientific, Waltham, MA) Instrument Name: pH Sensor Instrument Short Name:pH Sensor Instrument Description: An instrument that measures the hydrogen ion activity in solutions.
The overall concentration of hydrogen ions is inversely related to its pH. The pH scale ranges from 0 to 14 and indicates whether acidic (more H+) or basic (less H+).
Metrohm 809
Metrohm 809
PI Supplied Instrument Name: Metrohm 809 PI Supplied Instrument Description:Alkalinity was measured using automated Gran titration (Metrohm 809; Metrohm, Herisau, Switzerland) Instrument Name: Titrator Instrument Short Name:Titrator Instrument Description: Titrators are instruments that incrementally add quantified aliquots of a reagent to a sample until the end-point of a chemical reaction is reached. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB12/
Accumet Excel XL60
Accumet Excel XL60
PI Supplied Instrument Name: Accumet Excel XL60 PI Supplied Instrument Description:Seawater pH (NBS) and temperature were quantified using a pH/temperature meter: Accumet Excel XL60 (Thermo Fisher Scientific, Waltham, MA) Instrument Name: Water Temperature Sensor Instrument Short Name:Water Temp Sensor Instrument Description: General term for an instrument that measures the temperature of the water with which it is in contact (thermometer). Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/134/
Deployment: BML_Gaylord
BML_Gaylord
lab Bodega Marine Laboratory
laboratory
BML_Gaylord
Brian Gaylord
University of California - Davis: Bodega Marine Laboratory
lab Bodega Marine Laboratory
laboratory