http://lod.bco-dmo.org/id/dataset/659426
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2016-09-20
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Tank conditions for pH experiments on Lophelia pertusa specimens collected in the Norwegian Skagerrak and the Gulf of Mexico (Lophelia OA project)
2016-09-20
publication
2016-09-20
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2019-05-10
publication
https://doi.org/10.1575/1912/bco-dmo.659426.1
Erik E. Cordes
Temple University
principalInvestigator
Dr Robert J. Kulathinal
Temple University
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Cordes, E., Kulathinal, R. (2016) Tank conditions for pH experiments on Lophelia pertusa specimens collected in the Norwegian Skagerrak and the Gulf of Mexico (Lophelia OA project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2016-09-20 [if applicable, indicate subset used]. doi:10.1575/1912/bco-dmo.659426.1 [access date]
Tank conditions for pH experiments on L. pertusa. Dataset Description: <p>Data describing tank conditions from pH manipulation experiments on <em>Lophelia </em><em>pertusa</em><em>.</em> Specimens were collected from Tisler&nbsp;Reef in the Norwegian&nbsp;Skagerrak and from the Gulf of Mexico.</p>
<p><strong>Related References: Georgian S.E., et al. 2016</strong></p> Methods and Sampling: <p><strong>Acquisition Description for Tisler&nbsp;Reef Data:</strong></p>
<p>Net calcification was measured using the total alkalinity anomaly (Smith &amp; Key 1975; Ohde &amp; Hossain 2004). Corals were individually placed in closed glass chambers (220 ml) in a water bath that maintained temperature to +/-0.2 degrees C during all trials. To avoid hypoxia or the severe reductions of pH during incubations, ambient air was continuously bubbled into the chambers at a slow rate (1-2 bubbles s-1). This also provided adequate circulation within the chamber. A 60 ml water sample was collected by syringe before and after the incubation period, and measured for total alkalinity in duplicate. The respiration rate of each colony was measured as oxygen consumption in a&nbsp;400 ml closed acrylic chamber during hour-long incubations. Dissolved oxygen concentrations were measured in&nbsp;umol&nbsp;L-1&nbsp;using a Strathkelvin 782 dual oxygen meter and SI130&nbsp;microcathode&nbsp;electrode. The feeding rate of each colony was measured as the capture rate of adult&nbsp;<em>Artemia salina&nbsp;</em>during a one-hour period in 0.8 L incubation chambers containing a starting prey density of 125&nbsp;<em>Artemia</em>&nbsp;L-1.</p>
<p><strong>Acquisition Description for Gulf of Mexico Data:</strong></p>
<p>The buoyant weight of each colony was obtained at the start and end of the two-week experimental period by weighing fragments submerged in seawater and attached by a hook to an analytical balance (Denver Instrument,&nbsp;precision&nbsp;of 0.1 mg). The respiration rate of each colony was measured as oxygen consumption in an 800 ml closed acrylic chamber during hour-long incubations. Dissolved oxygen concentrations were measured in&nbsp;umol&nbsp;L-1&nbsp;using a Strathkelvin 782 dual oxygen meter and SI130&nbsp;microcathode&nbsp;electrode. The feeding rate of each colony was measured as the capture rate of adult&nbsp;<em>Artemia salina&nbsp;</em>during a one-hour period in 0.8 L incubation chambers containing a starting prey density of 125&nbsp;<em>Artemia</em>&nbsp;L-1.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1220478 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1220478
completed
Erik E. Cordes
Temple University
215-204-8876
Temple University Biology-Life Sciences 315
Philadelphia
PA
19122
USA
ecordes@temple.edu
pointOfContact
Dr Robert J. Kulathinal
Temple University
215-204-0620
Temple University Biology-Life Sciences 214
Philadelphia
PA
19122
USA
robkulathinal@temple.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
location
pH_treatment
tank
DO
ttl_alkalinity
salinity
temperature
pH
aragonite_saturation_state
day
SI130 microcathode electrode
Strathkelvin 782 dual oxygen meter
pH sensor
Salinity sensor
Temperature sensor
theme
None, User defined
site
treatment
tank
dissolved Oxygen
total alkalinity (TA)
salinity
temperature of sample or a generic measurement (not in-situ water temp)
pH
Aragonite Saturation State
day of month
featureType
BCO-DMO Standard Parameters
Oxygen Microelectrode Sensor
Oxygen Sensor
pH Sensor
Salinity Sensor
Water Temperature Sensor
instrument
BCO-DMO Standard Instruments
Gulf_of_Mexico
Tisler_Reef
service
Deployment Activity
Gulf of Mexico
Norwegian Skagerrak
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Science, Engineering and Education for Sustainability NSF-Wide Investment (SEES): Ocean Acidification (formerly CRI-OA)
https://www.nsf.gov/funding/pgm_summ.jsp?pims_id=503477
Science, Engineering and Education for Sustainability NSF-Wide Investment (SEES): Ocean Acidification (formerly CRI-OA)
NSF Climate Research Investment (CRI) activities that were initiated in 2010 are now included under Science, Engineering and Education for Sustainability NSF-Wide Investment (SEES). SEES is a portfolio of activities that highlights NSF's unique role in helping society address the challenge(s) of achieving sustainability. Detailed information about the SEES program is available from NSF (https://www.nsf.gov/funding/pgm_summ.jsp?pims_id=504707).
In recognition of the need for basic research concerning the nature, extent and impact of ocean acidification on oceanic environments in the past, present and future, the goal of the SEES: OA program is to understand (a) the chemistry and physical chemistry of ocean acidification; (b) how ocean acidification interacts with processes at the organismal level; and (c) how the earth system history informs our understanding of the effects of ocean acidification on the present day and future ocean.
Solicitations issued under this program:
NSF 10-530, FY 2010-FY2011
NSF 12-500, FY 2012
NSF 12-600, FY 2013
NSF 13-586, FY 2014
NSF 13-586 was the final solicitation that will be released for this program.
PI Meetings:
1st U.S. Ocean Acidification PI Meeting(March 22-24, 2011, Woods Hole, MA)
2nd U.S. Ocean Acidification PI Meeting(Sept. 18-20, 2013, Washington, DC)
3rd U.S. Ocean Acidification PI Meeting (June 9-11, 2015, Woods Hole, MA – Tentative)
NSF media releases for the Ocean Acidification Program:
Press Release 10-186 NSF Awards Grants to Study Effects of Ocean Acidification
Discovery Blue Mussels "Hang On" Along Rocky Shores: For How Long?
Discovery nsf.gov - National Science Foundation (NSF) Discoveries - Trouble in Paradise: Ocean Acidification This Way Comes - US National Science Foundation (NSF)
Press Release 12-179 nsf.gov - National Science Foundation (NSF) News - Ocean Acidification: Finding New Answers Through National Science Foundation Research Grants - US National Science Foundation (NSF)
Press Release 13-102 World Oceans Month Brings Mixed News for Oysters
Press Release 13-108 nsf.gov - National Science Foundation (NSF) News - Natural Underwater Springs Show How Coral Reefs Respond to Ocean Acidification - US National Science Foundation (NSF)
Press Release 13-148 Ocean acidification: Making new discoveries through National Science Foundation research grants
Press Release 13-148 - Video nsf.gov - News - Video - NSF Ocean Sciences Division Director David Conover answers questions about ocean acidification. - US National Science Foundation (NSF)
Press Release 14-010 nsf.gov - National Science Foundation (NSF) News - Palau's coral reefs surprisingly resistant to ocean acidification - US National Science Foundation (NSF)
Press Release 14-116 nsf.gov - National Science Foundation (NSF) News - Ocean Acidification: NSF awards $11.4 million in new grants to study effects on marine ecosystems - US National Science Foundation (NSF)
SEES-OA
largerWorkCitation
program
Physiological and genetic responses of the deep-water coral, Lophelia pertusa, to ongoing ocean acidification in the Gulf of Mexico
https://osprey.bco-dmo.org/project/2224
Physiological and genetic responses of the deep-water coral, Lophelia pertusa, to ongoing ocean acidification in the Gulf of Mexico
<p>The Gulf of Mexico deep water ecosystems are threatened by the persistent threat of ocean acidification. Deep-water corals will be among the first to feel the effects of this process, in particular the deep-water scleractinians that form their skeleton from aragonite. The continued shoaling of the aragonite saturation horizon (the depth below which aragonite is undersaturated) will place many of the known, and as yet undiscovered, deep-water corals at risk in the very near future. The most common deep-water framework-forming scleractinian in the world's oceans is <em>Lophelia pertusa</em>. This coral is most abundant in the North Atlantic, where aragonite saturation states are relatively high, but it also creates extensive reef structures between 300 and 600 m depth in the Gulf of Mexico where aragonite saturation states were previously unknown. Preliminary data indicate that pH at this depth range is between 7.85 and 8.03, and the aragonite saturation state is typically between 1.28 and 1.69. These are the first measurements of aragonite saturation state for the deep Gulf of Mexico, and are among the lowest Aragonite saturation state yet recorded for framework-forming corals in any body of water, at any depth.</p>
<p>This project will examine the effects of ocean acidification on<em> L. pertusa</em>, combining laboratory experiments, rigorous oceanographic measurements, the latest genome and transcriptome sequencing platforms, and quantitative PCR and enzyme assays to examine changes in coral gene expression and enzyme activity related to differences in carbonate chemistry. Short-term and long-term laboratory experiments will be performed at Aragonite saturation state of 1.45 and 0.75 and the organismal (e.g., survivorship and calcification rate) and genetic (e.g., transcript abundance) responses of the coral will be monitored. Genomic DNA and RNA will be extracted, total mRNA purified, and comprehensive and quantitative profiles of the transcriptome generated using a combination of 454 and Illumina sequencing technologies. Key genes in the calcification pathways as well as other differentially expressed genes will be targeted for specific qPCR assays to verify the Illumina sequencing results. On a research cruise,<em> L. pertusa</em> will be sampled (preserved at depth) along a natural gradient in carbonate chemistry, and included in the Illumina sequencing and qPCR assays. Water samples will be obtained by submersible-deployed niskin bottles adjacent to the coral collections as well as CTD casts of the water column overlying the sites. Water samples will be analyzed for pH, alkalinity, nitrates and soluble reactive phosphorus. These will be used in combination with historical data in a model to hindcast Aragonite saturation state.</p>
<p>This project will provide new physiological and genetic data on an ecologically-significant and anthropogenically-threatened deepwater coral in the Gulf of Mexico. An experimental system, already developed by the PIs, offers controlled conditions to test the effect of Aragonite saturation state on calcification rates in scleractinians and, subsequently, to identify candidate genes and pathways involved in the response to reduced pH and Aragonite saturation state. Both long-term and population sampling experiments will provide additional transcriptomic data and specifically investigate the expression of the candidate genes. These results will contribute to our understanding of the means by which scleractinians may acclimate and acclimatize to low pH, alkalinity, and Aragonite saturation state. Furthermore, the investigators will continue a time series of oceanographic measurements of the carbonate system in the Gulf of Mexico, which will allow the inclusion of this significant body of water in models of past and future ocean acidification scenarios.</p>
Lophelia OA
largerWorkCitation
project
eng; USA
oceans
Gulf of Mexico; Norwegian Skagerrak
2016-09-20
Northern Gulf of Mexico
0
BCO-DMO catalogue of parameters from Tank conditions for pH experiments on Lophelia pertusa specimens collected in the Norwegian Skagerrak and the Gulf of Mexico (Lophelia OA project)
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/659600.rdf
Name: location
Units: unitless
Description: <p>Location where specimen was collected; Tisler Reef or the Gulf of Mexico</p>
http://lod.bco-dmo.org/id/dataset-parameter/659601.rdf
Name: pH_treatment
Units: unitless
Description: <p>Level of pH treatment</p>
http://lod.bco-dmo.org/id/dataset-parameter/659602.rdf
Name: tank
Units: unitless
Description: <p>Tank number</p>
http://lod.bco-dmo.org/id/dataset-parameter/659603.rdf
Name: DO
Units: micromoles per kilogram (umol/kg)
Description: <p>Dissolved oxygen level in tank</p>
http://lod.bco-dmo.org/id/dataset-parameter/659604.rdf
Name: ttl_alkalinity
Units: micromoles per kilogram (umol/kg)
Description: <p>Total alkanlinity of tank</p>
http://lod.bco-dmo.org/id/dataset-parameter/659605.rdf
Name: salinity
Units: practical salinity unit (PSU)
Description: <p>Salinity of water in tank</p>
http://lod.bco-dmo.org/id/dataset-parameter/659606.rdf
Name: temperature
Units: celsius
Description: <p>Temperature of water in tank</p>
http://lod.bco-dmo.org/id/dataset-parameter/659607.rdf
Name: pH
Units: unitless
Description: <p>pH level of water in tank</p>
http://lod.bco-dmo.org/id/dataset-parameter/659608.rdf
Name: aragonite_saturation_state
Units: unitless
Description: <p>saturation state of aragonite</p>
http://lod.bco-dmo.org/id/dataset-parameter/765583.rdf
Name: day
Units: dimensionless
Description: <p>day of month</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
7821
https://darchive.mblwhoilibrary.org/bitstream/1912/24115/1/dataset-659426_tank-conditions-l-pertusa-ph-experiments__v1.tsv
download
https://doi.org/10.1575/1912/bco-dmo.659426.1
download
onLine
dataset
<p><strong>Acquisition Description for Tisler&nbsp;Reef Data:</strong></p>
<p>Net calcification was measured using the total alkalinity anomaly (Smith &amp; Key 1975; Ohde &amp; Hossain 2004). Corals were individually placed in closed glass chambers (220 ml) in a water bath that maintained temperature to +/-0.2 degrees C during all trials. To avoid hypoxia or the severe reductions of pH during incubations, ambient air was continuously bubbled into the chambers at a slow rate (1-2 bubbles s-1). This also provided adequate circulation within the chamber. A 60 ml water sample was collected by syringe before and after the incubation period, and measured for total alkalinity in duplicate. The respiration rate of each colony was measured as oxygen consumption in a&nbsp;400 ml closed acrylic chamber during hour-long incubations. Dissolved oxygen concentrations were measured in&nbsp;umol&nbsp;L-1&nbsp;using a Strathkelvin 782 dual oxygen meter and SI130&nbsp;microcathode&nbsp;electrode. The feeding rate of each colony was measured as the capture rate of adult&nbsp;<em>Artemia salina&nbsp;</em>during a one-hour period in 0.8 L incubation chambers containing a starting prey density of 125&nbsp;<em>Artemia</em>&nbsp;L-1.</p>
<p><strong>Acquisition Description for Gulf of Mexico Data:</strong></p>
<p>The buoyant weight of each colony was obtained at the start and end of the two-week experimental period by weighing fragments submerged in seawater and attached by a hook to an analytical balance (Denver Instrument,&nbsp;precision&nbsp;of 0.1 mg). The respiration rate of each colony was measured as oxygen consumption in an 800 ml closed acrylic chamber during hour-long incubations. Dissolved oxygen concentrations were measured in&nbsp;umol&nbsp;L-1&nbsp;using a Strathkelvin 782 dual oxygen meter and SI130&nbsp;microcathode&nbsp;electrode. The feeding rate of each colony was measured as the capture rate of adult&nbsp;<em>Artemia salina&nbsp;</em>during a one-hour period in 0.8 L incubation chambers containing a starting prey density of 125&nbsp;<em>Artemia</em>&nbsp;L-1.</p>
Specified by the Principal Investigator(s)
<p><strong>Processing Description for Tisler Reef Data:</strong></p>
<p>Net calcification was calculated as&nbsp;umol&nbsp;CaCO3&nbsp;gTW-1&nbsp;h-1&nbsp;using the following formula: Calcification = 0.5 * V * [(DeltaTAF) - (DeltaTAC)] / T * TW, where V is the volume of seawater in liters, DeltaTAF&nbsp;is the average change in total alkalinity during the incubation of each fragment, DeltaTAC&nbsp;is the average change in total alkalinity during the control incubations, T is the incubation time in hours, and TW is the final tissue weight of each fragment in grams. To allow for comparison to other studies, we then calculated net calcification as percent starting weight day-1. Capture rate was standardized to polyp number and reported as&nbsp;<em>Artemia&nbsp;</em>polyp-1&nbsp;h-1.</p>
<p><strong>Processing Description for Gulf of Mexico Data:&nbsp;</strong></p>
<p>Buoyant weights were converted to dry weights in air using the density of the skeleton and of the seawater. A correction for the contribution of tissue to buoyant weights (following Davies 1989) was applied to obtain the dry weight of the skeleton alone. Net calcification was then calculated as the change in skeletal dry weight over the two-week experimental period, expressed as % starting weight day-1. Capture rate was standardized to polyp number and reported as&nbsp;<em>Artemia&nbsp;</em>polyp-1&nbsp;h-1.</p>
<p><strong>Data Management Office Notes:</strong></p>
<p>-Separate spreadsheets in the&nbsp;original file (one for each location) have been combined and served as one object.<br />
-Re-formatted column names to comply with BCO-DMO naming standards.&nbsp;</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
SI130 microcathode electrode
SI130 microcathode electrode
PI Supplied Instrument Name: SI130 microcathode electrode PI Supplied Instrument Description:Measured dissolved oxygen concentrations Instrument Name: Oxygen Microelectrode Sensor Instrument Short Name:O2 Microelectrode Instrument Description: Any microelectrode sensor that measures oxygen. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/351/
Strathkelvin 782 dual oxygen meter
Strathkelvin 782 dual oxygen meter
PI Supplied Instrument Name: Strathkelvin 782 dual oxygen meter PI Supplied Instrument Description:Measured dissolved oxygen concentrations Instrument Name: Oxygen Sensor Instrument Short Name:Dissolved Oxygen Sensor Instrument Description: An electronic device that measures the proportion of oxygen (O2) in the gas or liquid being analyzed
pH sensor
pH sensor
PI Supplied Instrument Name: pH sensor PI Supplied Instrument Description:Indicates pH of water Instrument Name: pH Sensor Instrument Short Name:pH Sensor Instrument Description: An instrument that measures the hydrogen ion activity in solutions.
The overall concentration of hydrogen ions is inversely related to its pH. The pH scale ranges from 0 to 14 and indicates whether acidic (more H+) or basic (less H+).
Salinity sensor
Salinity sensor
PI Supplied Instrument Name: Salinity sensor PI Supplied Instrument Description:Indicates salinity of water Instrument Name: Salinity Sensor Instrument Short Name:Salinity Sensor Instrument Description: Category of instrument that simultaneously measures electrical conductivity and temperature in the water column to provide temperature and salinity data. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/350/
Temperature sensor
Temperature sensor
PI Supplied Instrument Name: Temperature sensor PI Supplied Instrument Description:Indicates water temperature Instrument Name: Water Temperature Sensor Instrument Short Name:Water Temp Sensor Instrument Description: General term for an instrument that measures the temperature of the water with which it is in contact (thermometer). Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/134/
Deployment: Gulf_of_Mexico
Gulf_of_Mexico
lab Cordes
laboratory
Gulf_of_Mexico
Erik E. Cordes
Temple University
Deployment: Tisler_Reef
Tisler_Reef
lab Cordes
laboratory
Tisler_Reef
Erik E. Cordes
Temple University
lab Cordes
laboratory