19:I[42426,["/_next/static/immutable/chunks/1xctgzqre-wsy.js","/_next/static/immutable/chunks/06_-wz6pdtp2b.js","/_next/static/immutable/chunks/30e52g49hg40p.js","/_next/static/immutable/chunks/02yq6tul2z-oh.js","/_next/static/immutable/chunks/10bx_ec2vj7n_.js","/_next/static/immutable/chunks/33bfn03fo8xnt.js","/_next/static/immutable/chunks/0tfjf78scu61b.js","/_next/static/immutable/chunks/31xnvej4n1bi9.js"],"default"] 1a:T4bc,Small and large capacity vacuum pump samplers. May have single or multiple chambers. Slurp Samplers (from https://ndsf.whoi.edu/alvin/systems/) The slurp samplers consist of a collection chamber and a hydraulically powered water pump. A nozzle with a tube connected to it is held and moved by a manipulator while water is pulled into the nozzle by the pump. The water travels down the tube into the collection chamber, and then goes through a mesh screen before going through the pump itself. Biological samples are sucked into the nozzle and deposited in the collection chamber. They are kept from going into the pump by the mesh screen. The screen can be changed for coarser or finer mesh as needed. Two styles of collection chamber are available; single chamber and 5-chamber. Single chamber: a large cylindrical single collection chamber into which all samples will be deposited. Water weight 2 lbs, 27 lbs full of water. 5-chamber: five separate cylindrical collection chambers of 4.5 inch ID x 11.5 inch height. One chamber is used at a time and the chambers are rotated by a hydraulic actuator mounted to the bottom of the 5-chamber assembly. Water weight 10 lbs, 140 lbs full of water.1b:Tee6,The Gulf of Mexico deep water ecosystems are threatened by the persistent threat of ocean acidification. Deep-water corals will be among the first to feel the effects of this process, in particular the deep-water scleractinians that form their skeleton from aragonite. The continued shoaling of the aragonite saturation horizon (the depth below which aragonite is undersaturated) will place many of the known, and as yet undiscovered, deep-water corals at risk in the very near future. The most common deep-water framework-forming scleractinian in the world's oceans is Lophelia pertusa. This coral is most abundant in the North Atlantic, where aragonite saturation states are relatively high, but it also creates extensive reef structures between 300 and 600 m depth in the Gulf of Mexico where aragonite saturation states were previously unknown. Preliminary data indicate that pH at this depth range is between 7.85 and 8.03, and the aragonite saturation state is typically between 1.28 and 1.69. These are the first measurements of aragonite saturation state for the deep Gulf of Mexico, and are among the lowest Aragonite saturation state yet recorded for framework-forming corals in any body of water, at any depth. This project will examine the effects of ocean acidification on L. pertusa, combining laboratory experiments, rigorous oceanographic measurements, the latest genome and transcriptome sequencing platforms, and quantitative PCR and enzyme assays to examine changes in coral gene expression and enzyme activity related to differences in carbonate chemistry. Short-term and long-term laboratory experiments will be performed at Aragonite saturation state of 1.45 and 0.75 and the organismal (e.g., survivorship and calcification rate) and genetic (e.g., transcript abundance) responses of the coral will be monitored. Genomic DNA and RNA will be extracted, total mRNA purified, and comprehensive and quantitative profiles of the transcriptome generated using a combination of 454 and Illumina sequencing technologies. Key genes in the calcification pathways as well as other differentially expressed genes will be targeted for specific qPCR assays to verify the Illumina sequencing results. On a research cruise, L. pertusa will be sampled (preserved at depth) along a natural gradient in carbonate chemistry, and included in the Illumina sequencing and qPCR assays. Water samples will be obtained by submersible-deployed niskin bottles adjacent to the coral collections as well as CTD casts of the water column overlying the sites. Water samples will be analyzed for pH, alkalinity, nitrates and soluble reactive phosphorus. These will be used in combination with historical data in a model to hindcast Aragonite saturation state. This project will provide new physiological and genetic data on an ecologically-significant and anthropogenically-threatened deepwater coral in the Gulf of Mexico. An experimental system, already developed by the PIs, offers controlled conditions to test the effect of Aragonite saturation state on calcification rates in scleractinians and, subsequently, to identify candidate genes and pathways involved in the response to reduced pH and Aragonite saturation state. Both long-term and population sampling experiments will provide additional transcriptomic data and specifically investigate the expression of the candidate genes. These results will contribute to our understanding of the means by which scleractinians may acclimate and acclimatize to low pH, alkalinity, and Aragonite saturation state. Furthermore, the investigators will continue a time series of oceanographic measurements of the carbonate system in the Gulf of Mexico, which will allow the inclusion of this significant body of water in models of past and future ocean acidification scenarios.6:["$","$L19",null,{"dataset":{"_id":"659440","description":"

Sample logs from HOV Alvin dives (AL4704-AL4716) on R/V Atlantis (AT26-14) between April - May 2014 in the Gulf of Mexico.

\n","problem":"","acquisition":"

Sample logs were acquired for each dive. UTM coordinates, lat/lons, depths, and sampling descriptions are included.

\n","processing":"

Data Management Office Processing Notes:
\n-Combined separate deployment files into one table.
\n-Re-formatted column names to comply with BCO-DMO naming standards.

\n","bcodmoProcessing":"","title":"Sample logs from R/V Atlantis (AT26-14) Alvin dives in the Gulf of Mexico during 2014 (Lophelia OA project)","nickname":"AT26-14 Alvin sample logs","dataUrl":"","currentState":"Final no updates expected","currentStateId":"7","validated":true,"restricted":false,"version":"1","version_date":"2016-09-20T04:00:00.000Z","abstract":"Sample logs from R/V Atlantis (AT26-14) Alvin dives in the Gulf of Mexico during 2014 (Lophelia OA project)","citation":"Cordes, E. (2016) Sample logs from R/V Atlantis (AT26-14) Alvin dives in the Gulf of Mexico during 2014 (Lophelia OA project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2016-09-20 [if applicable, indicate subset used]. doi:10.1575/1912/bco-dmo.659440.1 [access date]","citationParts":{"identifier":"doi:10.1575/1912/bco-dmo.659440.1","versionDate":"2016-09-20","versionId":"1","year":"2016","title":"Sample logs from R/V Atlantis (AT26-14) Alvin dives in the Gulf of Mexico during 2014 (Lophelia OA project)","authors":"Cordes, E.","publisher":"Biological and Chemical Oceanography Data Management Office (BCO-DMO)."},"doi":"10.1575/1912/bco-dmo.659440.1","release_date":null,"field_dataset_location":"","temporal_extent":{"start":"2014-04-30T04:00:00.000Z","end":"2014-05-14T04:00:00.000Z"},"temporal":{"resolution":"time:unitDay","type":"time:Interval","start":{"value":"1398830400","label":"2014-04-30"},"end":{"value":"1400040000","label":"2014-05-14"}},"spatial_extent":{"shape":"polygon","center":{"latitude":28.12740111,"longitude":-90.805496735},"wkt":"POLYGON ((-93.59891607 27.08029275, -88.0120774 27.08029275, -88.0120774 29.17450947, -93.59891607 29.17450947, -93.59891607 27.08029275))","north":29.17450947,"east":-88.0120774,"south":27.08029275,"west":-93.59891607},"dataset_type":["Cruise Results"],"keywords":["oceans"],"data_files":[{"id":"901179","title":"sample_logs.csv","url":"https://datadocs.bco-dmo.org/dataset/659440/file/m77kmRNcRPkZNN/sample_logs.csv","showTableUrl":"https://www.bco-dmo.org/dataset/659440/data/view/901179","description":"Primary data file for dataset ID 659440","filename":"sample_logs.csv","bytesize":54301,"checksum":{"value":"3cbaa3b90316be5ff82a7a5bab8d1544","Algorithm":{"_id":"190","name":"MD5"}},"mimetype":"text/csv","fileType":{"_id":"192","name":"Comma Separated Values (.csv)"},"isErddap":"bcodmo_dataset_659440","date":"","isPublicDuringEmbargo":false,"primaryDataFile":true,"showTable":true,"restricted":false}],"supplemental_files":[],"roles":[{"role":{"_id":"138","name":"Principal Investigator"},"performedBy":{"_id":"51539","name":"Erik E. Cordes"},"performedAtOrganization":{"_id":"294","name":"Temple University"}},{"role":{"_id":"133","name":"Contact"},"performedBy":{"_id":"51539","name":"Erik E. Cordes"},"performedAtOrganization":{"_id":"294","name":"Temple University"}},{"role":{"_id":"145","name":"BCO-DMO Data Manager"},"performedBy":{"_id":"650173","name":"Hannah Ake"},"performedAtOrganization":{"_id":"191","name":"Woods Hole Oceanographic Institution"}}],"deployments":[{"_id":"658949","name":"AT26-14","synonyms":[],"dates":{"start":"2014-04-27T00:00:00.000Z","end":"2014-05-16T00:00:00.000Z"},"platform":{"_id":"54003","name":"R/V Atlantis"},"chiefScientists":[{"id":"51539","name":"Erik E. Cordes","role":"Chief Scientist"}]},{"_id":"659523","name":"AT26-14_Alvin_Dives","synonyms":[],"dates":{"start":"2014-04-27T00:00:00.000Z","end":"2014-05-14T00:00:00.000Z"},"platform":{"_id":"54226","name":"HOV Alvin"},"chiefScientists":[{"id":"51539","name":"Erik E. Cordes","role":"Chief Scientist"}]}],"funding":[{"_id":"54992","name":"OCE-1220478","fundingSource":{"_id":"355","name":"NSF Division of Ocean Sciences"},"url":"https://www.nsf.gov/awardsearch/show-award?AWD_ID=1220478"}],"instruments":[{"_id":"659475","name":"Pump","description":"Pump used to collect samples from seafloor or water column","type_id":"644821","type_name":"HOV Alvin Slurp Samplers","type_desc":"$1a","type_acronym":""},{"_id":"659476","name":"Niskin","description":"Used to collect water samples","type_id":"413","type_name":"Niskin bottle","type_desc":"A Niskin bottle (a next generation water sampler based on the Nansen bottle) is a cylindrical, non-metallic water collection device with stoppers at both ends. The bottles can be attached individually on a hydrowire or deployed in 12, 24, or 36 bottle Rosette systems mounted on a frame and combined with a CTD. Niskin bottles are used to collect discrete water samples for a range of measurements including pigments, nutrients, plankton, etc.","type_acronym":"Niskin bottle"}],"parameters":[{"_id":"659457","name":"dive","format":"","frictionlessType":"","description":"

Dive ID number

","units":{"_id":"","name":"unitless"},"type":{"_id":"1734","name":"dive_id","description":"

Unique dive id or number for ROV, AUV or HOV type system dive or deployment

"}},{"_id":"659458","name":"sampleLog_date","format":"","frictionlessType":"","description":"

Date sample was taken; mm/dd/yy

","units":{"_id":"","name":"unitless"},"type":{"_id":"1070","name":"date","description":"

date; generally reported in GMT as YYYYMMDD (year; month; day); also as MMDD (month; day); EqPac dates are local Hawaii time. ISO_Date format is YYYY-MM-DD (http://www.iso.org/iso/home/standards/iso8601.htm)

"}},{"_id":"659459","name":"sampleLog_time","format":"","frictionlessType":"","description":"

Time sample was taken; HH:MM

","units":{"_id":"","name":"unitless"},"type":{"_id":"1075","name":"time","description":"

Time GMT defined as HHMM; time of day; generally reported in GMT as HHMM (hours and minutes) but may also be HH.H (decimal hours) or HHMMSS (hours; minutes and seconds); in EqPac time is reported as Hawaii local time

"}},{"_id":"659460","name":"sampleLog_seconds","format":"","frictionlessType":"","description":"

Seconds into dive sampling occurs; SSSSS

","units":{"_id":"","name":"seconds"},"type":{"_id":"1096","name":"seconds_local","description":"

seconds of time, local

"}},{"_id":"659462","name":"sampleLog_sample","format":"","frictionlessType":"","description":"

Sampling method

","units":{"_id":"","name":"unitless"},"type":{"_id":"960","name":"sample","description":"

unique sample identification or number; any combination of alpha numeric characters; precise definition is file dependent

"}},{"_id":"659463","name":"sampleLog_location","format":"","frictionlessType":"","description":"

Sampling location

","units":{"_id":"","name":"unitless"},"type":{"_id":"1124","name":"site","description":"

Sampling site identification.

"}},{"_id":"659464","name":"sampleLog_pump","format":"","frictionlessType":"","description":"

Sampling pump; on or off

","units":{"_id":"","name":"unitless"},"type":{"_id":"960","name":"sample","description":"

unique sample identification or number; any combination of alpha numeric characters; precise definition is file dependent

"}},{"_id":"659465","name":"sampleLog_species","format":"","frictionlessType":"","description":"

Species sampled

","units":{"_id":"","name":"unitless"},"type":{"_id":"976","name":"species","description":"

a taxonomic binomial that consists of a genus name followed by the species name of an organism

"}},{"_id":"659466","name":"sampleLog_notes","format":"","frictionlessType":"","description":"

Notes on sampling

","units":{"_id":"","name":"unitless"},"type":{"_id":"778","name":"comment","description":"

free text comments, may only have meaning to submitting PI

"}},{"_id":"659468","name":"time","format":"","frictionlessType":"","description":"

Time sample was taken as it occurs in footage; HH:MM:SS

","units":{"_id":"","name":"unitless"},"type":{"_id":"1075","name":"time","description":"

"}},{"_id":"659469","name":"lat","format":"","frictionlessType":"","description":"

Latitude

","units":{"_id":"","name":"decimal degrees"},"type":{"_id":"730","name":"latitude","description":"

latitude, in decimal degrees, North is positive, negative denotes South; Reported in some datasets as degrees, minutes

"}},{"_id":"659470","name":"lon","format":"","frictionlessType":"","description":"

Longitude

","units":{"_id":"","name":"decimal degrees"},"type":{"_id":"731","name":"longitude","description":"

longitude, in decimal degrees, East is positive, negative denotes West; Reported in some datsets as degrees, minutes

"}},{"_id":"659471","name":"utmx","format":"","frictionlessType":"","description":"

Universal Transverse Mercator coordinate system X coordinates

","units":{"_id":"","name":"meters"},"type":{"_id":"659439","name":"UTM Coordinates","description":"

The Universal Transverse Mercator (UTM) conformal projection uses a 2-dimensional Cartesian coordinate system to give locations on the surface of the Earth. The UTM system allows the coordinate numbering system to be tied directly to a distance measuring system. All coordinates are measured in meters.

"}},{"_id":"659472","name":"utmy","format":"","frictionlessType":"","description":"

Universal Transverse Mercator coordinate system Y coordinates

","units":{"_id":"","name":"meters"},"type":{"_id":"659439","name":"UTM Coordinates","description":"

"}},{"_id":"659473","name":"depth","format":"","frictionlessType":"","description":"

Depth of sample

","units":{"_id":"","name":"meters"},"type":{"_id":"808","name":"depth","description":"

Observation/sample depth below the sea surface. Units often reported as: meters, feet.

\nWhen used in a JGOFS/GLOBEC dataset the depth is a best estimate; usually but not always calculated from pressure; calculated either from CTD pressure using Fofonoff and Millard (1982; UNESCO Tech Paper #44) algorithm adjusted for 1980 equation of state for seawater (EOS80) or simply equivalent to nominal depth as recorded during sampling if CTD pressure was unavailable.

"}},{"_id":"659474","name":"time_sec","format":"","frictionlessType":"","description":"

Seconds into dive the start of sampling occurs

","units":{"_id":"","name":"seconds"},"type":{"_id":"1096","name":"seconds_local","description":"

seconds of time, local

"}}],"related_publications":[],"related_datasets":[],"services":[{"grouping":"","items":[{"name":"View Data","description":"An in-browser view of the primary tabular data file","url":"https://www.bco-dmo.org/dataset/659440/data/view","contentType":"text/html","actionType":"view"}]},{"grouping":"ERDDAP","items":[{"name":"SUBSET DATA","description":"","url":"https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_659440.html","contentType":"text/html","actionType":"tool"},{"name":"VIEW TABLE","description":"","url":"https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_659440.htmlTable","contentType":"text/html","actionType":"view"},{"name":"CSV","description":"","url":"https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_659440.csv","contentType":"text/csv","actionType":"download"},{"name":"TSV","description":"","url":"https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_659440.tsv","contentType":"text/tsv","actionType":"download"},{"name":"GeoJSON","description":"","url":"https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_659440.geoJson","contentType":"application/geo+json","actionType":"download"},{"name":"MATLAB","description":"","url":"https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_659440.mat","contentType":"text/x-matlab","actionType":"download"},{"name":"netCDF","description":"","url":"https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_659440.nc","contentType":"application/x-netcdf","actionType":"download"}]},{"grouping":"Metadata","items":[{"name":"HTML","description":"A simple HTML page of the dataset metadata","url":"https://www.bco-dmo.org/dataset/659440/description","contentType":"text/html","actionType":"view"},{"name":"PDF","description":"A PDF of the HTML dataset metadata","url":"https://www.bco-dmo.org/dataset/659440/Dataset_description.pdf","contentType":"application/pdf","actionType":"download"},{"name":"ISO 19115-2 (NOAA Profile)","description":"","url":"https://www.bco-dmo.org/dataset/659440/iso","contentType":"application/xml","actionType":"download"}]}],"programs":[{"_id":"2030","name":"Science, Engineering and Education for Sustainability NSF-Wide Investment (SEES): Ocean Acidification (formerly CRI-OA)","acronym":"SEES-OA"}],"projects":[{"_id":"2224","name":"Physiological and genetic responses of the deep-water coral, Lophelia pertusa, to ongoing ocean acidification in the Gulf of Mexico","acronym":"Lophelia OA","description":"$1b"}],"archives":[{"id_value":"10.1575/1912/bco-dmo.659440.1","url":"https://doi.org/10.1575/1912/bco-dmo.659440.1","date_assigned":"2019-04-24","version_date":"2016-09-20","archiver":"Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)","archiver_id":"272"}],"license":{"name":"Creative Commons Attribution 4.0","id":"CC-BY-4.0","uri":"https://creativecommons.org/licenses/by/4.0/","spdxUri":"https://spdx.org/licenses/CC-BY-4.0"},"metrics":{"views":{"count":140},"downloads":{"count":371}}}}]