http://lod.bco-dmo.org/id/dataset/660158
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2016-09-29
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Results of laboratory study examining effects of elevated concentrations of seawater carbon dioxide and altered salinity on rates of oxygen utilization by larval porcelain crabs (Petrolisthes cinctipes)
2016-09-29
publication
2016-09-29
revision
BCO-DMO Linked Data URI
2016-09-29
creation
http://lod.bco-dmo.org/id/dataset/660158
Brian Gaylord
University of California - Davis: Bodega Marine Laboratory
principalInvestigator
Tessa M. Hill
University of California - Davis: Bodega Marine Laboratory
principalInvestigator
Ann D. Russell
University of California-Davis
principalInvestigator
Eric Sanford
University of California - Davis: Bodega Marine Laboratory
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Gaylord, B., Hill, T. M., Sanford, E., Russell, A. D. (2016) Results of laboratory study examining effects of elevated concentrations of seawater carbon dioxide and altered salinity on rates of oxygen utilization by larval porcelain crabs (Petrolisthes cinctipes). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 29 Sept 2016) Version Date 2016-09-29 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/660158 [access date]
Carbonate system conditions during salinity treatments for crab respirometry Dataset Description: <p>Laboratory results concerning effects of elevated concentrations of seawater carbon dioxide and altered salinity on rates of oxygen utilization by larval porcelain crabs (<em>Petrolisthes cinctipes</em>). This dataset includes the carbonate system conditions during salinity treatments for crab respirometry</p>
<p>Related datasets:<br />
<a href="http://www.bco-dmo.org/dataset/659950" target="_blank">oxygen treatment data</a><br />
<a href="http://www.bco-dmo.org/dataset/660065" target="_blank">oxygen drawdown</a><br />
<a href="http://www.bco-dmo.org/dataset/660131" target="_blank">culturing system carbonate chemistry</a></p> Methods and Sampling: <p>In this study, the investigators&nbsp;determined how respiration by newly hatched&nbsp;larvae of the porcelain crab (<em>Petrolisthes cinctipes</em>) cultured under&nbsp;current (385 uatm) and future predicted (1000 uatm) levels of&nbsp;atmospheric pCO2 differed when exposed to low, normal,&nbsp;and high&nbsp;salinity conditions.</p>
<p><strong>Detailed methodology and results are described in following publication:</strong><br />
Miller, S.H., S. Zarate, E.H. Smith, B. Gaylord, J.D. Hosfelt, and T.M. Hill. 2014. Effect of elevated <em>p</em>CO<sub>2</sub> on metabolic responses of porcelain crab (<em>Petrolisthes cinctipes</em>) larvae exposed to subsequent salinity stress. PLoS One 9: e109167, doi:<a href="http://dx.doi.org/10.1371/journal.pone.0109167" target="_blank">10.1371/journal.pone.0109167</a></p>
<p><strong>Briefly (excerpted from above):</strong><br />
Fifty ovigerous Petrolisthes cinctipes were collected from Shell Beach (38 degrees 25.033' N, 123 degrees 06.350' W) and Twin Coves (38 degrees 27.490' N, 123 degrees 08.621' W), California.&nbsp;Crabs were held in individual mesh containers in flow-through seawater at Bodega Marine Laboratory for 2–14 days. Containers were checked daily for hatched larvae, and newly released individuals&nbsp;from multiple broods were transferred in mixed batches of 75 to replicate culture jars. These jars contained 0.45 um filtered seawater bubbled with prescribed pCO2 levels to maintain carbonate chemistry and water movement, and were held at a constant temperature and normal salinity. Not all females released larvae on the same night, which allowed culture start dates to be staggered and respirometry trials to be run on sequential days using larvae of the same age.</p>
<p>Larvae were cultured at 14 degrees&nbsp;C under two pCO2 conditions: 385 uatm (ambient) and 1000 uatm (elevated). Larvae were held at a density of 75 larvae per 3 L, and were fed newly hatched Artemia sp. nauplii at a density of 1 nauplius/ml. The water in each culture jar was changed every two days. During&nbsp;water changes, dead larvae and uneaten Artemia sp. were removed, and water exiting the jars was sampled for carbonate chemistry adhering to recommended practices. The investigators measured pH (total scale) using a glass electrode (Accumet Excel XL60; Thermo Fisher Scientific, Waltham, Massachusetts, USA) and repeated analysis of certified TRIS reference material (measured in millivolts; batch no. 8; A. Dickson, Scripps Institute of Oceanography). Total alkalinity (TA) was measured by autotitration (Metrohm 809; Metrohm, Herisau, Switzerland), using the same certified reference material. Dissolved inorganic carbon (DIC) samples were processed using coulometric titration at the Monterey Bay Aquarium Research Institute (MBARI).</p>
<p>Respirometry trials were conducted in seawater at ambient pCO2 using a dissolved oxygen meter (model 781b Strathkelvin Instruments Ltd., Glasgow, UK) with a Clark-type microcathode polarographic electrode with a 22 micron diameter platinum cathode and silver/silver chloride anode connected by a buffered potassium chloride electrolyte solution (model 1302). Data were&nbsp;transmitted via a data interface unit to a computer and the oxygen consumption rates were monitored using the Strathkelvin 949 Oxygen System (Version 2.2, Strathkelvin Instruments Ltd., Glasgow, UK).</p>
<p>The electrode was placed in a PVC holder with double O rings to seal the chamber. The holder and electrode were inserted into a test chamber (26.34 mm x 47.09 mm ID) holding 25 ml of test&nbsp;solution. The test chamber was maintained at a temperature of 14 degrees C. For each new trial, the electrode was calibrated using 2% sodium sulfite and air-saturated seawater to determine zero and saturated oxygen values, respectively. Before each series of experiments, a control chamber&nbsp;with no larvae was monitored to establish background microbial oxygen consumption rates, which were then subtracted from the values obtained during trial runs. During each trial, eight first-stage larvae were removed from the culturing system and placed in the chamber for respirometry analysis, and visual analysis indicated that their activity was sufficient to maintain flow over the surface of the electrode. Seawater oxygen saturation state was measured every two minutes for one hour, though the investigators only analyzed data from the start of the trial until the&nbsp;oxygen in the test chamber reached 5 mg/L to eliminate potential effects of hypoxia in the chamber on larval respiration. Trials with larvae from each pCO2 treatment were run sequentially in pairs (i.e., one trial with larvae from ambient pCO2 conditions was followed by one trial with larvae from elevated pCO2 conditions), and 13 pairs of trials were run at salinities of 34 over a period of 14 days.</p>
<p>Distilled water was added to filtered, ambient-pCO2 seawater (salinity 34) to make salinity 22 solution, and artificial seawater (Instant Ocean, Spectrum Brands, Inc.) was added to make salinity 40 solution. This procedure created two altered salinity treatments for testing effects of salinity stress on larvae drawn from each of the preceding pCO2 treatments. Larvae were allowed to acclimatize in treatment solutions for ten minutes prior to respirometry trials to attenuate a startle response, and the system was calibrated before each trial. Paired trials (4 pairs at salinity 22 and 3 pairs at salinity 40) with larvae from each pCO2 treatment were run sequentially over a period of seven days.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-0927255 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=0927255
completed
Brian Gaylord
University of California - Davis: Bodega Marine Laboratory
707-875-1940
Bodega Marine Laboratory & Department of Evolution and Ecology 2099 Westshore Road, P.O. Box 247
Bodega Bay
CA
94923
USA
bpgaylord@ucdavis.edu
pointOfContact
Tessa M. Hill
University of California - Davis: Bodega Marine Laboratory
P.O. Box 247 2099 Westshore Road
Bodega Bay
CA
94923
USA
tmhill@ucdavis.edu
pointOfContact
Ann D. Russell
University of California-Davis
Department of Geology One Shields Avenue University of California Davis
Davis
CA
95616
USA
adrussell@ucdavis.edu
pointOfContact
Eric Sanford
University of California - Davis: Bodega Marine Laboratory
P.O. Box 247 2099 Westshore Road
Bodega Bay
CA
94923
USA
edsanford@ucdavis.edu
pointOfContact
asNeeded
Dataset Version: 29 Sept 2016
Unknown
sample
sal
pH
pCO2
alk
omega_aragonite
DIC
Accumet Excel XL60
781b Strathkelvin Instruments Ltd.
Metrohm 809
theme
None, User defined
sample identification
salinity
pH
Partial pressure of CO2
total alkalinity (TA)
Aragonite Saturation State
dissolved inorganic Carbon
featureType
BCO-DMO Standard Parameters
Benchtop pH Meter
Oxygen Sensor
Titrator
instrument
BCO-DMO Standard Instruments
BML_Gaylord
service
Deployment Activity
Bodega Marine Lab, UC Davis
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Bodega Ocean Acidification Research
http://bml.ucdavis.edu/research/research-programs/climate-change/oceanacidification/
Bodega Ocean Acidification Research
<p>The absorption of human-produced CO2 into the world's oceans is decreasing seawater pH and causing marked declines in the saturation state for calcium carbonate, a major building block for shells, skeletons, and tests of many marine species. Such changes (collectively termed "ocean acidification") have the potential to devastate a broad array of organisms, both at the level of individuals and at population and ecosystem scales. Although awareness of these issues is rapidly growing, most of what is known is based on studies of coral reef organisms and plankton.</p>
<p>The proposed work will enhance understanding of impacts from ocean acidification by providing rigorous data on several new fronts applicable to temperate systems. The project will operate within one of the strongest upwelling centers of the eastern Pacific, where global trends in acidification are amplified by the presence of cold water characterized by already-high levels of aqueous CO2. Using an integrated, comparative approach that exploits the expertise of oceanographers, marine chemists, and biologists, the project will explicitly couple moored and shipboard measurements of seawater chemistry to controlled laboratory and field studies of biological responses.</p>
<p>Two vital foundation species (the California mussel, <em>Mytilus californianus</em>, and the Olympia oyster, <em>Ostrea conchaphila</em>) will be targeted. These two species play disproportionately important roles in open-coast and estuarine systems, respectively. Larvae (which are often the most vulnerable stages) of mussels and oysters will be cultured under elevated-CO2 conditions through the full pelagic period and into juvenile life. Growth and survivorship will be quantified, and water temperature and salinity will be varied to test for interactive effects of multiple factors. Intraspecific variation in response of larvae from different parental lineages will be examined. "Carry-over" effects that originate from exposure during the larval stage, but influence subsequent juvenile growth and survival, will be determined both in the laboratory and using field outplants. Because larval and juvenile stages play important roles as demographic age-structure bottlenecks, overall population consequences will be estimated through comparison of observed impacts on early life stages to other recognized sources of recruitment variation.</p>
<p><strong>Data Status:</strong> Data will be reported from the BML offshore oceanographic moorings and from moorings within nearby Tomales Bay. The moorings will be outfitted with autonomously recording pH and pCO2 sensors, and these measurements will be supplemented with discrete water samples collected monthly along two associated transects.</p>
<p><strong>Live Data:</strong> For live-streaming data from Tomales Bay, visit <a href="http://www.ipacoa.org/Explorer" target="_blank">http://www.ipacoa.org/Explorer</a> and click on the icon in Tomales Bay.</p>
BOAR
largerWorkCitation
project
eng; USA
oceans
Bodega Marine Lab, UC Davis
2016-09-29
Central California coast (northeast Pacific)
0
BCO-DMO catalogue of parameters from Results of laboratory study examining effects of elevated concentrations of seawater carbon dioxide and altered salinity on rates of oxygen utilization by larval porcelain crabs (Petrolisthes cinctipes)
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/660181.rdf
Name: sample
Units: unitless
Description: <p>Sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/660182.rdf
Name: sal
Units: practical salinity units
Description: <p>Salinity</p>
http://lod.bco-dmo.org/id/dataset-parameter/660183.rdf
Name: pH
Units: pH total scale
Description: <p>pH</p>
http://lod.bco-dmo.org/id/dataset-parameter/660184.rdf
Name: pCO2
Units: microatmospheres (uatm)
Description: <p>pCO2</p>
http://lod.bco-dmo.org/id/dataset-parameter/660185.rdf
Name: alk
Units: micromoles per kilogram (umol/kg)
Description: <p>Alkalinity</p>
http://lod.bco-dmo.org/id/dataset-parameter/660186.rdf
Name: omega_aragonite
Units: dimensionless
Description: <p>Aragonite saturation state</p>
http://lod.bco-dmo.org/id/dataset-parameter/660187.rdf
Name: DIC
Units: micromoles per kilogram (umol/kg)
Description: <p>Dissolved inorganic carbon (DIC)</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
223
https://datadocs.bco-dmo.org/file/0AAMNqmuMz1vP1/crab_treatment_chem.csv
crab_treatment_chem.csv
Primary data file for dataset ID 660158
download
https://osprey.bco-dmo.org/dataset/660158/data/download
download
onLine
dataset
<p>In this study, the investigators&nbsp;determined how respiration by newly hatched&nbsp;larvae of the porcelain crab (<em>Petrolisthes cinctipes</em>) cultured under&nbsp;current (385 uatm) and future predicted (1000 uatm) levels of&nbsp;atmospheric pCO2 differed when exposed to low, normal,&nbsp;and high&nbsp;salinity conditions.</p>
<p><strong>Detailed methodology and results are described in following publication:</strong><br />
Miller, S.H., S. Zarate, E.H. Smith, B. Gaylord, J.D. Hosfelt, and T.M. Hill. 2014. Effect of elevated <em>p</em>CO<sub>2</sub> on metabolic responses of porcelain crab (<em>Petrolisthes cinctipes</em>) larvae exposed to subsequent salinity stress. PLoS One 9: e109167, doi:<a href="http://dx.doi.org/10.1371/journal.pone.0109167" target="_blank">10.1371/journal.pone.0109167</a></p>
<p><strong>Briefly (excerpted from above):</strong><br />
Fifty ovigerous Petrolisthes cinctipes were collected from Shell Beach (38 degrees 25.033' N, 123 degrees 06.350' W) and Twin Coves (38 degrees 27.490' N, 123 degrees 08.621' W), California.&nbsp;Crabs were held in individual mesh containers in flow-through seawater at Bodega Marine Laboratory for 2–14 days. Containers were checked daily for hatched larvae, and newly released individuals&nbsp;from multiple broods were transferred in mixed batches of 75 to replicate culture jars. These jars contained 0.45 um filtered seawater bubbled with prescribed pCO2 levels to maintain carbonate chemistry and water movement, and were held at a constant temperature and normal salinity. Not all females released larvae on the same night, which allowed culture start dates to be staggered and respirometry trials to be run on sequential days using larvae of the same age.</p>
<p>Larvae were cultured at 14 degrees&nbsp;C under two pCO2 conditions: 385 uatm (ambient) and 1000 uatm (elevated). Larvae were held at a density of 75 larvae per 3 L, and were fed newly hatched Artemia sp. nauplii at a density of 1 nauplius/ml. The water in each culture jar was changed every two days. During&nbsp;water changes, dead larvae and uneaten Artemia sp. were removed, and water exiting the jars was sampled for carbonate chemistry adhering to recommended practices. The investigators measured pH (total scale) using a glass electrode (Accumet Excel XL60; Thermo Fisher Scientific, Waltham, Massachusetts, USA) and repeated analysis of certified TRIS reference material (measured in millivolts; batch no. 8; A. Dickson, Scripps Institute of Oceanography). Total alkalinity (TA) was measured by autotitration (Metrohm 809; Metrohm, Herisau, Switzerland), using the same certified reference material. Dissolved inorganic carbon (DIC) samples were processed using coulometric titration at the Monterey Bay Aquarium Research Institute (MBARI).</p>
<p>Respirometry trials were conducted in seawater at ambient pCO2 using a dissolved oxygen meter (model 781b Strathkelvin Instruments Ltd., Glasgow, UK) with a Clark-type microcathode polarographic electrode with a 22 micron diameter platinum cathode and silver/silver chloride anode connected by a buffered potassium chloride electrolyte solution (model 1302). Data were&nbsp;transmitted via a data interface unit to a computer and the oxygen consumption rates were monitored using the Strathkelvin 949 Oxygen System (Version 2.2, Strathkelvin Instruments Ltd., Glasgow, UK).</p>
<p>The electrode was placed in a PVC holder with double O rings to seal the chamber. The holder and electrode were inserted into a test chamber (26.34 mm x 47.09 mm ID) holding 25 ml of test&nbsp;solution. The test chamber was maintained at a temperature of 14 degrees C. For each new trial, the electrode was calibrated using 2% sodium sulfite and air-saturated seawater to determine zero and saturated oxygen values, respectively. Before each series of experiments, a control chamber&nbsp;with no larvae was monitored to establish background microbial oxygen consumption rates, which were then subtracted from the values obtained during trial runs. During each trial, eight first-stage larvae were removed from the culturing system and placed in the chamber for respirometry analysis, and visual analysis indicated that their activity was sufficient to maintain flow over the surface of the electrode. Seawater oxygen saturation state was measured every two minutes for one hour, though the investigators only analyzed data from the start of the trial until the&nbsp;oxygen in the test chamber reached 5 mg/L to eliminate potential effects of hypoxia in the chamber on larval respiration. Trials with larvae from each pCO2 treatment were run sequentially in pairs (i.e., one trial with larvae from ambient pCO2 conditions was followed by one trial with larvae from elevated pCO2 conditions), and 13 pairs of trials were run at salinities of 34 over a period of 14 days.</p>
<p>Distilled water was added to filtered, ambient-pCO2 seawater (salinity 34) to make salinity 22 solution, and artificial seawater (Instant Ocean, Spectrum Brands, Inc.) was added to make salinity 40 solution. This procedure created two altered salinity treatments for testing effects of salinity stress on larvae drawn from each of the preceding pCO2 treatments. Larvae were allowed to acclimatize in treatment solutions for ten minutes prior to respirometry trials to attenuate a startle response, and the system was calibrated before each trial. Paired trials (4 pairs at salinity 22 and 3 pairs at salinity 40) with larvae from each pCO2 treatment were run sequentially over a period of seven days.</p>
Specified by the Principal Investigator(s)
<p><strong>BCO-DMO processing:</strong><br />
- modified parameter names to conform with BCO-DMO naming conventions;<br />
- replaced missing data with "nd" ("no data").</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Accumet Excel XL60
Accumet Excel XL60
PI Supplied Instrument Name: Accumet Excel XL60 PI Supplied Instrument Description:pH (total scale) was measured using a glass electrode (Accumet Excel XL60; Thermo Fisher Scientific, Waltham, Massachusetts, USA) Instrument Name: Benchtop pH Meter Instrument Short Name:Benchtop pH Meter Instrument Description: An instrument consisting of an electronic voltmeter and pH-responsive electrode that gives a direct conversion of voltage differences to differences of pH at the measurement temperature. (McGraw-Hill Dictionary of Scientific and Technical Terms)
This instrument does not map to the NERC instrument vocabulary term for 'pH Sensor' which measures values in the water column. Benchtop models are typically employed for stationary lab applications.
781b Strathkelvin Instruments Ltd.
781b Strathkelvin Instruments Ltd.
PI Supplied Instrument Name: 781b Strathkelvin Instruments Ltd. PI Supplied Instrument Description:Respirometry trials were conducted in seawater at ambient pCO2 using a dissolved oxygen meter (model 781b Strathkelvin
Instruments Ltd., Glasgow, UK) with a Clark-type microcathode polarographic electrode with a 22 micron diameter platinum
cathode and silver/silver chloride anode connected by a buffered potassium chloride electrolyte solution (model 1302). Instrument Name: Oxygen Sensor Instrument Short Name:Dissolved Oxygen Sensor Instrument Description: An electronic device that measures the proportion of oxygen (O2) in the gas or liquid being analyzed
Metrohm 809
Metrohm 809
PI Supplied Instrument Name: Metrohm 809 PI Supplied Instrument Description:Total alkalinity (TA) was measured by autotitration (Metrohm 809; Metrohm, Herisau, Switzerland). Instrument Name: Titrator Instrument Short Name:Titrator Instrument Description: Titrators are instruments that incrementally add quantified aliquots of a reagent to a sample until the end-point of a chemical reaction is reached. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB12/
Deployment: BML_Gaylord
BML_Gaylord
lab Bodega Marine Laboratory
laboratory
BML_Gaylord
Brian Gaylord
University of California - Davis: Bodega Marine Laboratory
lab Bodega Marine Laboratory
laboratory