http://lod.bco-dmo.org/id/dataset/660543
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2016-10-04
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Water column data from CTD casts along the East Siberian Arctic Shelf on R/V Oden during 2011 (ESAS Water Column Methane project)
2016-10-04
publication
2016-10-04
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2019-04-22
publication
https://doi.org/10.1575/1912/bco-dmo.660543.1
Samantha B. Joye
University of Georgia
principalInvestigator
Vladimir Samarkin
University of Georgia
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Joye, S., Samarkin, V. (2016) Water column data from CTD casts along the East Siberian Arctic Shelf on R/V Oden during 2011 (ESAS Water Column Methane project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2016-10-04 [if applicable, indicate subset used]. doi:10.1575/1912/bco-dmo.660543.1 [access date]
CTD water column data from the East Siberian Arctic Shelf. Dataset Description: <p>These data are from&nbsp;water column samples collected by CTD from the Lappet Sea, East Siberian Arctic Shelf in 2011.&nbsp;</p> Methods and Sampling: <p><strong>Acquisition methods are described in the following publication: Orcut, B. <em>et al. 2005</em></strong></p>
<p><strong>Core sectioning, porewater&nbsp;collection&nbsp;and analysis</strong></p>
<p>At each sampling site, sediment sub-samples were collected for porewater analyses and at selected depths for microbial rate assays (AOM, anaerobic oxidation of methane oxidation; methanogenesis (MOG) from bicarbonate and acetate). Sediment was expelled from core liner using a hydraulic extruder under anoxic conditions. The depth intervals for extrusion varied. At each depth interval, a sub-sample was collected into a cut-off syringe for dissolved methane concentration quantification. Another 5 mL&nbsp;sub-sample&nbsp;was collected into pre-weighed and pre-combusted glass vial for determination of porosity (determined by the change in weight after drying at 80 degrees celsius to a constant weight). The remaining material was used for porewater extraction. Sample fixation and&nbsp;analyses&nbsp;for dissolved constituents followed the methods of Joye et al. (2010).&nbsp;</p>
<p><strong>Microbial Activity Measurements&nbsp;</strong></p>
<p>To determine AOM and MOG rates, 8 to 12 sub-samples (5 cm3) were collected from a core by manual insertion of a glass tube. For AOM, 100 uL of dissolved&nbsp;14CH4&nbsp;tracer (about 2,000,000 DPM as gas) was injected into each core. Samples were incubated for 36 to 48 hours at&nbsp;in situ&nbsp;temperature.&nbsp; Following incubation, samples were transferred to 20 mL glass vials containing 2 mL of 2M NaOH (which served to arrest biological activity and fix&nbsp;14CO2&nbsp;as&nbsp;14C-HCO3-).&nbsp; Each vial was sealed with a&nbsp;teflon-lined screw cap, vortexed to mix the sample and base, and immediately frozen. Time zero samples were fixed immediately after radiotracer injection. The specific activity of the tracer substrate (14CH4) was determined by injecting 50 uL directly into scintillation cocktail (Scintiverse BD) followed by liquid scintillation counting. The accumulation of 14C product (14CO2) was determined by acid digestion following the method of Joye et al. (2010).&nbsp; The AOM rate was calculated using equation 1:</p>
<p>AOM Rate = [CH4] x alphaCH4 /t x (a-14CO2/a-14CH4)&nbsp;&nbsp; &nbsp;&nbsp;&nbsp; &nbsp;&nbsp;&nbsp; &nbsp;(Eq. 1)</p>
<p>Here, the AOM Rate is expressed as nmol CH4 oxidized per cm3 sediment per day (nmol&nbsp;cm-3 d-1), [CH4] is the methane concentration (uM), alphaCH4 is the isotope fractionation factor for AOM (1.06; (ALPERIN and REEBURGH, 1988)), t is the incubation time (d), a-14CO2 is the activity of the product pool, and a-14CH4 is the activity of the substrate pool. If methane concentration was not available, the turnover time of the 14CH4 tracer is presented.</p>
<p>Rates of bicarbonate-based-methanogenesis and acetoclastic methanogenesis were determined by incubating samples in gas-tight, closed-tube vessels without headspace, to prevent the loss of gaseous 14CH4 product during sample manipulation. These sample tubes were sealed using custom-designed plungers (black Hungate stoppers with the lip removed containing a plastic “tail” that was run through the stopper) were inserted at the base of the tube; the sediment was then pushed via the plunger to the top of the tube until a small amount protruded through the tube opening. A butyl rubber septa&nbsp;was&nbsp;then eased into the tube opening to displace sediment in contact with the atmosphere and close the tube, which was then sealed with&nbsp;a open-top&nbsp;screw cap.&nbsp; The rubber materials used in these assays were boiled in 1N NaOH for 1 hour, followed by several rinses in boiling milliQ, to leach potentially toxic substances. &nbsp; &nbsp;</p>
<p>A volume of radiotracer solution (100 uL of 14C-HCO3- tracer (~1 x 107&nbsp;dpm&nbsp;in slightly alkaline milliQ&nbsp;water) or 1,2-14C-CH3COO- tracer (~5 x 107&nbsp;dpm&nbsp;in slightly alkaline milliQ&nbsp;water)) was injected into each sample. Samples were incubated as described above and then 2 ml of 2N NaOH was injected through the top stopper into each sample to terminate biological activity (time zero samples were fixed prior to tracer injection).&nbsp; Samples were mixed to evenly distribute NaOH through the sample.&nbsp; Production of 14CH4 was quantified by stripping methane from the tubes with an air carrier, converting the 14CH4 to 14CO2 in a combustion furnace, and subsequent trapping of the 14CO2 in NaOH as carbonate (CRAGG et al., 1990; CRILL and MARTENS, 1986).&nbsp;&nbsp;Activity&nbsp;of 14CO2 was measured subsequently by liquid scintillation counting.&nbsp;</p>
<p>The rates of Bi-MOG and Ac-MOG rates were calculated using equations 2 and 3, respectively:</p>
<p>Bi-MOG Rate = [HCO3-] x alphaHCO3/t x&nbsp; (a-14CH4/a-H14CO3-) &nbsp; &nbsp; (Eq. 2)</p>
<p>Ac-MOG Rate = [CH3COO-] x alphaCH3COO-/t&nbsp; x&nbsp; (a-14CH4/a-14CH314COO-) &nbsp; &nbsp; (Eq. 3)</p>
<p>Both rates are expressed as nmol HCO3- or CH3COO-, respectively, reduced cm-3 d-1, alphaHCO3&nbsp;and alphaCH3COO- are the isotope fractionation factors for MOG (assumed to be 1.06). [HCO3-] and [CH3COO-] are the&nbsp;pore&nbsp;water bicarbonate (mM) and acetate (uM) concentrations, respectively, t is incubation time (d), a-14CH4 is the activity of the product pool, and a-H14CO3 and a-14CH314COO are the activities of the substrate pools. If samples for substrate concentration determination were not available, the substrate turnover constant instead of the rate is presented.</p>
<p>For water column methane oxidation rate assays, triplicate 20 mL of live water (in addition to one 20 mL sample which was killed with ethanol (750 uL of pure EtOH) before tracer addition) were transferred from the CTD into serum vials. Samples were amended with 2 x 10^6 DPM of 3H-labeled-methane tracer and incubated for 24 to 72 hours (linearity of activity was tested and confirmed). After incubation, samples were fixed with ethanol, as above, and a sub-sample to determine total sample activity (3H-methane + 3H-water) was collected. Next, the sample was purged with nitrogen to remove the 3H-methane tracer and a sub-sample was amended with scintillation fluid and counted on a shipboard scintillation counter to determine the activity of tracer in the product of 3H-methane oxidation, 3H-water. The methane oxidation rate was calculated as:</p>
<p>MOX Rate = [methane concentration in nM] x alphaCH4/t&nbsp; x&nbsp; (a-3H-H2O/a-3H-CH4-) &nbsp; &nbsp; (Eq. 3)</p>
Funding provided by NSF Division of Polar Programs (NSF PLR) Award Number: PLR-1023444 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1023444
completed
Samantha B. Joye
University of Georgia
706-542-5893
Dept. of Marine Sciences 325 Sanford Drive
Athens
GA
30602
USA
mjoye@uga.edu
pointOfContact
Vladimir Samarkin
University of Georgia
(706) 542-3614
Department of Marine Sciences University of Georgia
Athens
Georgia
30602
United States
samarkin@uga.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
station
date
lon
lat
depth_max
depth_sample
temperature
pH
salinity
Cl
SO4
DOC
TDN
DON
Nox
NO2
NH4
TDP
DOP
PO4
Si
CH4
MOX
CTD
theme
None, User defined
station
date
longitude
latitude
depth_max
depth
temperature of sample or a generic measurement (not in-situ water temp)
pH
salinity
No BCO-DMO term
sulfate
dissolved organic Carbon
Total Dissolved Nitrogen
Dissolved Organic Nitrogen
Nitrite
Ammonium
Total Dissolved Phosphorus
Dissolved Organic Phosphorus
reactive phosphorus (PO4)
Silicon
featureType
BCO-DMO Standard Parameters
CTD - profiler
instrument
BCO-DMO Standard Instruments
ESAS_Fall_2011
ESAS_Spring_2011
service
Deployment Activity
East Siberian Arctic Shelf
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
The East Siberian Arctic Shelf as a Source of Atmospheric Methane: First Approach to Quantitative Assessment
https://osprey.bco-dmo.org/project/651769
The East Siberian Arctic Shelf as a Source of Atmospheric Methane: First Approach to Quantitative Assessment
<p>We propose to study methane (CH4) release over the East Siberian Arctic shelf (ESAS), the largest (~10% of the world ocean shelf area) and the shallowest shelf (mean depth <50 m) of the world ocean. Until recently, the ESAS was not considered a CH4 source due to subsea permafrost’s impermeability, which completely isolated it from modern biogeochemical cycles. The ESAS stores the world’s largest hydrocarbon stocks, mostly as shallow Arctic hydrates, and thus represents an enormous potential CH4 atmospheric source that could result from global warming-triggered permafrost degradation. Increased CH4 fluxes could occur as numerous weak seeps or strong bubble plumes over large areas. Due to the shallow nature of the ESAS, the majority of ESAS CH4 likely avoids oxidation and escapes to the atmosphere. To assess whether sudden, large-scale CH4 release occurs or is likely to occur in the future, we will investigate the migration pathway characteristics and identify the controlling factors of CH4 flux from the seabed, in the water column, and to the atmosphere.</p>
<p>Our central hypothesis is that seabed CH4 fluxes are significant year-round sources while atmospheric fluxes are only significant during ice-free periods. To understand both the temporal and spatial variability of CH4 fluxes, we will test the sub-hypotheses that ESAS emissions are strongly modulated by seasonal factors including water-column mixing and ice cover, while seabed CH4 emissions are much more weakly modulated (or not at all), and that the spatial distribution of emission correlates with the location of shallow and deep-water fault zones, and submerged thermokarst areas – primary geologic rather than biologic control. We will: 1) distinguish different CH4 sources 2) quantify the importance of microbial production and consumption of CH4 including seasonal variations vs. release of previously originated CH4 from seabed reservoirs; 3) evaluate contributions of different components (spatial, seasonal and transport components) to the annual CH4 flux; 4) investigate controlling factors and model CH4 release during critical periods such as storms, fall convection and ice-break up period and over critical area such as fault zones and thermokarst-affected areas; 5) use modeling to extend results to the entire ESAS and for climate change scenarios.</p>
<p>Water column hydrography and geochemical measurements will be conducted during a summer cruise and a winter expedition. To distinguish between individual CH4 sources we will measure stable carbon isotope ratios (δ13CCH4, δ13CDIC, δ13C-POC), D/H ratio of CH4 and water (δDCH4, δDH2O), CH4 radiocarbon age (Δ14C), and the abundance of non-CH4 hydrocarbons in water, air and sediment samples. To elucidate importance of microbial production (methanogenesis) we will estimate in situ production in particles; CH4 consumption (oxidation) will be measured in the water column and at the ice-water interface. To evaluate total flux, we will perform direct flux measurements (4 helicopter surveys) using eddy-correlation techniques to obtain integrative rates of CH4 flux and assess strength of the current CH4 source over two area. To assess contribution of ebullition, we will measure seafloor direct bubble flux over 6 control and 2 test sites on a nested range of sonar scales (3.5 kHz, largest), to multibeam sonar scanner (decameter), to turbine tents (meter), and a combination of bubble video imaging and bubble modeling (vent scale). To evaluate factors controlling CH4 fluxes, we will perform investigations of seabed permeability for gases using geophysics and 3He/4He ratio. Modeling will aim to develop a regional flux model.</p>
ESAS Water Column Methane
largerWorkCitation
project
eng; USA
oceans
East Siberian Arctic Shelf
125.0406
178.9479
65.0835
77.3829
2011-09-12
2011-10-07
East Siberian Arctic Shelf
0
BCO-DMO catalogue of parameters from Water column data from CTD casts along the East Siberian Arctic Shelf on R/V Oden during 2011 (ESAS Water Column Methane project)
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/660738.rdf
Name: station
Units: unitless
Description: <p>Station where sampling occurred</p>
http://lod.bco-dmo.org/id/dataset-parameter/660739.rdf
Name: date
Units: unitless
Description: <p>Date of sampling; YYYY/mm/dd</p>
http://lod.bco-dmo.org/id/dataset-parameter/660740.rdf
Name: lon
Units: decimal degrees
Description: <p>Longitude</p>
http://lod.bco-dmo.org/id/dataset-parameter/660741.rdf
Name: lat
Units: decimal degrees
Description: <p>Latitude</p>
http://lod.bco-dmo.org/id/dataset-parameter/660742.rdf
Name: depth_max
Units: meters
Description: <p>Max depth on site where sampling occurred</p>
http://lod.bco-dmo.org/id/dataset-parameter/660743.rdf
Name: depth_sample
Units: meters
Description: <p>Depth where sampling occurred</p>
http://lod.bco-dmo.org/id/dataset-parameter/660744.rdf
Name: temperature
Units: celsius
Description: <p>Temperature where sampling occurred</p>
http://lod.bco-dmo.org/id/dataset-parameter/660745.rdf
Name: pH
Units: pH
Description: <p>pH of water sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/660746.rdf
Name: salinity
Units: practical salinity units (PSU)
Description: <p>Salinity of water sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/660747.rdf
Name: Cl
Units: millimoles (mM)
Description: <p>Chlorine concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/660748.rdf
Name: SO4
Units: millimoles (mM)
Description: <p>Sulfate concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/660749.rdf
Name: DOC
Units: micromoles (uM)
Description: <p>Dissolved organic carbon concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/660750.rdf
Name: TDN
Units: micromoles (uM)
Description: <p>Total dissolved nitrogen concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/660751.rdf
Name: DON
Units: micromoles (uM)
Description: <p>Dissolved organic nitrogen concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/660752.rdf
Name: Nox
Units: micromoles (uM)
Description: <p>Mono-nitrogen oxide concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/660753.rdf
Name: NO2
Units: micromoles (uM)
Description: <p>Nitrite concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/660754.rdf
Name: NH4
Units: micromoles (uM)
Description: <p>Ammonium concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/660755.rdf
Name: TDP
Units: micromoles (uM)
Description: <p>Total dissolved phosphorus concetration</p>
http://lod.bco-dmo.org/id/dataset-parameter/660756.rdf
Name: DOP
Units: micromoles (uM)
Description: <p>Dissolved organic phosphorus concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/660757.rdf
Name: PO4
Units: micromoles (uM)
Description: <p>Phosphate concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/660758.rdf
Name: Si
Units: micromoles (uM)
Description: <p>Silicon concentation</p>
http://lod.bco-dmo.org/id/dataset-parameter/660759.rdf
Name: CH4
Units: micromoles (uM)
Description: <p>Methane concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/660760.rdf
Name: MOX
Units: nanomole per liter per day (nmol/L/d)
Description: <p>Methane oxidation rate</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
20351
https://darchive.mblwhoilibrary.org/bitstream/1912/24062/1/dataset-660543_water-column-data-ctd__v1.tsv
download
https://doi.org/10.1575/1912/bco-dmo.660543.1
download
onLine
dataset
<p><strong>Acquisition methods are described in the following publication: Orcut, B. <em>et al. 2005</em></strong></p>
<p><strong>Core sectioning, porewater&nbsp;collection&nbsp;and analysis</strong></p>
<p>At each sampling site, sediment sub-samples were collected for porewater analyses and at selected depths for microbial rate assays (AOM, anaerobic oxidation of methane oxidation; methanogenesis (MOG) from bicarbonate and acetate). Sediment was expelled from core liner using a hydraulic extruder under anoxic conditions. The depth intervals for extrusion varied. At each depth interval, a sub-sample was collected into a cut-off syringe for dissolved methane concentration quantification. Another 5 mL&nbsp;sub-sample&nbsp;was collected into pre-weighed and pre-combusted glass vial for determination of porosity (determined by the change in weight after drying at 80 degrees celsius to a constant weight). The remaining material was used for porewater extraction. Sample fixation and&nbsp;analyses&nbsp;for dissolved constituents followed the methods of Joye et al. (2010).&nbsp;</p>
<p><strong>Microbial Activity Measurements&nbsp;</strong></p>
<p>To determine AOM and MOG rates, 8 to 12 sub-samples (5 cm3) were collected from a core by manual insertion of a glass tube. For AOM, 100 uL of dissolved&nbsp;14CH4&nbsp;tracer (about 2,000,000 DPM as gas) was injected into each core. Samples were incubated for 36 to 48 hours at&nbsp;in situ&nbsp;temperature.&nbsp; Following incubation, samples were transferred to 20 mL glass vials containing 2 mL of 2M NaOH (which served to arrest biological activity and fix&nbsp;14CO2&nbsp;as&nbsp;14C-HCO3-).&nbsp; Each vial was sealed with a&nbsp;teflon-lined screw cap, vortexed to mix the sample and base, and immediately frozen. Time zero samples were fixed immediately after radiotracer injection. The specific activity of the tracer substrate (14CH4) was determined by injecting 50 uL directly into scintillation cocktail (Scintiverse BD) followed by liquid scintillation counting. The accumulation of 14C product (14CO2) was determined by acid digestion following the method of Joye et al. (2010).&nbsp; The AOM rate was calculated using equation 1:</p>
<p>AOM Rate = [CH4] x alphaCH4 /t x (a-14CO2/a-14CH4)&nbsp;&nbsp; &nbsp;&nbsp;&nbsp; &nbsp;&nbsp;&nbsp; &nbsp;(Eq. 1)</p>
<p>Here, the AOM Rate is expressed as nmol CH4 oxidized per cm3 sediment per day (nmol&nbsp;cm-3 d-1), [CH4] is the methane concentration (uM), alphaCH4 is the isotope fractionation factor for AOM (1.06; (ALPERIN and REEBURGH, 1988)), t is the incubation time (d), a-14CO2 is the activity of the product pool, and a-14CH4 is the activity of the substrate pool. If methane concentration was not available, the turnover time of the 14CH4 tracer is presented.</p>
<p>Rates of bicarbonate-based-methanogenesis and acetoclastic methanogenesis were determined by incubating samples in gas-tight, closed-tube vessels without headspace, to prevent the loss of gaseous 14CH4 product during sample manipulation. These sample tubes were sealed using custom-designed plungers (black Hungate stoppers with the lip removed containing a plastic “tail” that was run through the stopper) were inserted at the base of the tube; the sediment was then pushed via the plunger to the top of the tube until a small amount protruded through the tube opening. A butyl rubber septa&nbsp;was&nbsp;then eased into the tube opening to displace sediment in contact with the atmosphere and close the tube, which was then sealed with&nbsp;a open-top&nbsp;screw cap.&nbsp; The rubber materials used in these assays were boiled in 1N NaOH for 1 hour, followed by several rinses in boiling milliQ, to leach potentially toxic substances. &nbsp; &nbsp;</p>
<p>A volume of radiotracer solution (100 uL of 14C-HCO3- tracer (~1 x 107&nbsp;dpm&nbsp;in slightly alkaline milliQ&nbsp;water) or 1,2-14C-CH3COO- tracer (~5 x 107&nbsp;dpm&nbsp;in slightly alkaline milliQ&nbsp;water)) was injected into each sample. Samples were incubated as described above and then 2 ml of 2N NaOH was injected through the top stopper into each sample to terminate biological activity (time zero samples were fixed prior to tracer injection).&nbsp; Samples were mixed to evenly distribute NaOH through the sample.&nbsp; Production of 14CH4 was quantified by stripping methane from the tubes with an air carrier, converting the 14CH4 to 14CO2 in a combustion furnace, and subsequent trapping of the 14CO2 in NaOH as carbonate (CRAGG et al., 1990; CRILL and MARTENS, 1986).&nbsp;&nbsp;Activity&nbsp;of 14CO2 was measured subsequently by liquid scintillation counting.&nbsp;</p>
<p>The rates of Bi-MOG and Ac-MOG rates were calculated using equations 2 and 3, respectively:</p>
<p>Bi-MOG Rate = [HCO3-] x alphaHCO3/t x&nbsp; (a-14CH4/a-H14CO3-) &nbsp; &nbsp; (Eq. 2)</p>
<p>Ac-MOG Rate = [CH3COO-] x alphaCH3COO-/t&nbsp; x&nbsp; (a-14CH4/a-14CH314COO-) &nbsp; &nbsp; (Eq. 3)</p>
<p>Both rates are expressed as nmol HCO3- or CH3COO-, respectively, reduced cm-3 d-1, alphaHCO3&nbsp;and alphaCH3COO- are the isotope fractionation factors for MOG (assumed to be 1.06). [HCO3-] and [CH3COO-] are the&nbsp;pore&nbsp;water bicarbonate (mM) and acetate (uM) concentrations, respectively, t is incubation time (d), a-14CH4 is the activity of the product pool, and a-H14CO3 and a-14CH314COO are the activities of the substrate pools. If samples for substrate concentration determination were not available, the substrate turnover constant instead of the rate is presented.</p>
<p>For water column methane oxidation rate assays, triplicate 20 mL of live water (in addition to one 20 mL sample which was killed with ethanol (750 uL of pure EtOH) before tracer addition) were transferred from the CTD into serum vials. Samples were amended with 2 x 10^6 DPM of 3H-labeled-methane tracer and incubated for 24 to 72 hours (linearity of activity was tested and confirmed). After incubation, samples were fixed with ethanol, as above, and a sub-sample to determine total sample activity (3H-methane + 3H-water) was collected. Next, the sample was purged with nitrogen to remove the 3H-methane tracer and a sub-sample was amended with scintillation fluid and counted on a shipboard scintillation counter to determine the activity of tracer in the product of 3H-methane oxidation, 3H-water. The methane oxidation rate was calculated as:</p>
<p>MOX Rate = [methane concentration in nM] x alphaCH4/t&nbsp; x&nbsp; (a-3H-H2O/a-3H-CH4-) &nbsp; &nbsp; (Eq. 3)</p>
Specified by the Principal Investigator(s)
<p><strong>BCO-DMO Data Processing Notes:</strong></p>
<p>- filled in blank cells with "nd"<br />
- separated month and year into two columns<br />
- converted lat/lons to decimal degrees<br />
- replaced the code "MUC" with it's complete definition "multiple core"<br />
- replaced the code "BDL" with it's complete definition "below defined level"</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
CTD
CTD
PI Supplied Instrument Name: CTD PI Supplied Instrument Description:Used to collect water column samples Instrument Name: CTD - profiler Instrument Short Name: Instrument Description: The Conductivity, Temperature, Depth (CTD) unit is an integrated instrument package designed to measure the conductivity, temperature, and pressure (depth) of the water column. The instrument is lowered via cable through the water column. It permits scientists to observe the physical properties in real-time via a conducting cable, which is typically connected to a CTD to a deck unit and computer on a ship. The CTD is often configured with additional optional sensors including fluorometers, transmissometers and/or radiometers. It is often combined with a Rosette of water sampling bottles (e.g. Niskin, GO-FLO) for collecting discrete water samples during the cast.
This term applies to profiling CTDs. For fixed CTDs, see https://www.bco-dmo.org/instrument/869934. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/130/
Deployment: ESAS_Fall_2011
ESAS_Fall_2011
shoreside East Siberian Arctic Shelf
shoreside
ESAS_Fall_2011
Samantha B. Joye
University of Georgia
Deployment: ESAS_Spring_2011
ESAS_Spring_2011
shoreside East Siberian Arctic Shelf
shoreside
ESAS_Spring_2011
Samantha B. Joye
University of Georgia
shoreside East Siberian Arctic Shelf
shoreside