http://lod.bco-dmo.org/id/dataset/661354
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2016-10-11
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Carbon and Nitrogen values for low to high pCO2 acclimated Emiliania huxleyi (E Hux Response to pCO2 project)
2016-10-11
publication
2016-10-11
revision
BCO-DMO Linked Data URI
2016-10-11
creation
http://lod.bco-dmo.org/id/dataset/661354
M. Brady Olson
Western Washington University
principalInvestigator
Brooke Love
Western Washington University
principalInvestigator
Suzanne Strom
Western Washington University
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Olson, M. Brady, Love, B., Strom, S. (2016) Carbon and Nitrogen values for low to high pCO2 acclimated Emiliania huxleyi (E Hux Response to pCO2 project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). Version Date 2016-10-11 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/661354 [access date]
Carbon and Nitrogen values for low to high pCO2 acclimated E. huxleyi Dataset Description: <p><strong>Related Datasets:</strong><br />
<a href="http://www.bco-dmo.org/dataset/661464">Grazing experiments 2 and 3: cell volume</a><br />
<a href="http://www.bco-dmo.org/dataset/661405">Grazing experiments 2 and 3: daily cell counts</a><br />
<a href="http://www.bco-dmo.org/dataset/661421">Grazing experiments 2 and 3: ingestion</a><br />
<a href="http://www.bco-dmo.org/dataset/661388">Grazing experiments 2 and 3: pCO2</a></p>
<p><strong>Related Reference:</strong><br />
Kendall, K., Marine Microzooplankton are Indirectly Affected by Ocean Acidification Through Direct Effects on Their Phytoplankton Prey. (Masters Thesis) Western Washington University.<br />
<a href="http://cedar.wwu.edu/wwuet/448/">http://cedar.wwu.edu/wwuet/448/</a></p> Methods and Sampling: <p>The phytoplankton Emiliania huxleyi CCMP 2668 was grown semi-continuously in atmosphere controlled chambers at three different CO2 treatment concentrations; Ambient (400ppmv), Moderate (750ppmv), and High (1000ppmv).&nbsp; Cultures were diluted daily starting day 4 with pre-equilibrated media containing f/50 nutrients.&nbsp;</p>
<p>Expt. 2: On day 8, after ~ 16 generations, 200 mls of E. huxleyi cells from each of the treatment replicates was divided in to two 100 ml samples which were each filtered onto 20mm muffled glass fiber filters (GFF).</p>
<p>Expt. 3:&nbsp;On day 10, after ~ 20 generations, 200 mls of E. huxleyi cells from each of the treatment replicates was divided in to two 100 ml samples which were each filtered onto 20mm muffled glass fiber filters (GFF).</p>
<p>During this sampling, 5 mls of each treatment was fixed in alkaline Lugol’s for counting cells.&nbsp; One filter was used for TPC (total particulate carbon) and placed in a tin capsule the other was used for TOC and placed in a silver capsule.&nbsp; All samples were dried for 24 hours at 60°C.&nbsp; The tin capsules and filters were then folded and held in a desiccator until analysis.&nbsp; The silver capsules and filters were fumed in a closed container with concentrated sulfuric acid for 24 hours to remove the PIC contained in E. huxleyi’s coccoliths.&nbsp; The silver capsules and filters were then dried again at 60°C for 24 hrs, folded, and put in a tin capsule which was folded, then they were held in a desiccator until analysis.&nbsp; For analysis, folded capsules were combusted in a CE Elantech Flash EA 1112 elemental analyzer.&nbsp; Standard curves were made using known weights of Acetanilide wrapped in tin capsules. Media blanks, filter blanks and capsule blanks were included as controls for background signals.&nbsp; Cells were counted with a Hemocytometer or gridded Sedgewick Rafter Chamber, depending on cell density.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-0961229 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=0961229
completed
M. Brady Olson
Western Washington University
(360) 650-7400
1900 Shannon Point Rd.
Anacortes
WA
98221
United States
Brady.Olson@wwu.edu
pointOfContact
Brooke Love
Western Washington University
(360) 650-2894
516 High St. Mail Stop 9181
Bellingham
WA
98225
United States
brooke.love@wwu.edu
pointOfContact
Suzanne Strom
Western Washington University
360-293-2188
Shannon Point Marine Center 1900 Shannon Point Rd
Anacortes
WA
98221
USA
suzanne.strom@wwu.edu
pointOfContact
asNeeded
Unknown
expt
treatment
N_Peak_Area
C_peak_area
N_area_adj
C_area_adj
mg_N
mg_C
cells_ml
cells_filter
mg_N_cell
mg_C_cell
pg_N_cell
pg_TC_cell
pg_PON_cell
TC_N
pg_POC_cell
pg_PIC_cell
PIC_POC
POC_PON
PIC_PON
theme
None, User defined
experiment id
treatment
No BCO-DMO term
Total Particulate Nitrogen
total particulate Carbon
abundance
particulate organic nitrogen
Carbon to Nitrogen ratio (C:N)
particulate organic Carbon (POC)
particulate inorganic Carbon
featureType
BCO-DMO Standard Parameters
CHN Elemental Analyzer
instrument
BCO-DMO Standard Instruments
Lab_Olson_B
service
Deployment Activity
Bellingham, WA
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Planktonic interactions in a changing ocean: Biological responses of Emiliania huxleyi to elevated pCO2 and their effects on microzooplankton
https://osprey.bco-dmo.org/project/517694
Planktonic interactions in a changing ocean: Biological responses of Emiliania huxleyi to elevated pCO2 and their effects on microzooplankton
<p><em><strong>Description from NSF award abstract:</strong></em><br />
The calcifying Haptophyte <em>Emiliania huxleyi </em>appears to be acutely sensitive to the rising concentration of ocean pCO2. Documented responses by <em>E. huxleyi </em>to elevated pCO2 include modifications to their calcification rate and cell size, malformation of coccoliths, elevated growth rates, increased organic carbon production, lowering of PIC:POC ratios, and elevated production of the active climate gas DMS. Changes in these parameters are mechanisms known to elicit alterations in grazing behavior by microzooplankton, the oceans dominant grazer functional group. The investigators hypothesize that modifications to the physiology and biochemistry of calcifying and non-calcifying Haptophyte <em>Emiliania huxleyi </em>in response to elevated pCO2 will precipitate alterations in microzooplankton grazing dynamics. To test this hypothesis, they will conduct controlled laboratory experiments where several strains of <em>E. huxleyi</em> are grown at several CO2 concentrations. After careful characterization of the biochemical and physiological responses of the <em>E. huxleyi</em> strains to elevated pCO2, they will provide these strains as food to several ecologically-important microzooplankton and document grazing dynamics.<em> E. huxleyi</em> is an ideal organism for the study of phytoplankton and microzooplankton responses to rising anthropogenic CO2, the effects of which in the marine environment are called ocean acidification; <em>E. huxleyi </em>is biogeochemically important, is well studied, numerous strains are in culture that exhibit variation in the parameters described above, and they are readily fed upon by ecologically important microzooplankton.</p>
<p>The implications of changes in microzooplankton grazing for carbon cycling, specifically CaCO3 export, DMS production, nutrient regeneration in surface waters, and carbon transfer between trophic levels are profound, as this grazing, to a large degree, regulates all these processes. <em>E. huxleyi </em>is a model prey organism because it is one of the most biogeochemically influential global phytoplankton. It forms massive seasonal blooms, contributes significantly to marine inorganic and organic carbon cycles, is a large producer of the climatically active gas DMS, and is a source of organic matter for trophic levels both above and below itself. The planned controlled study will increase our knowledge of the mechanisms that drive patterns of change between trophic levels, thus providing a wider array of tools necessary to understand the complex nature of ocean acidification field studies, where competing variables can confound precise interpretation.</p>
E Hux Response to pCO2
largerWorkCitation
project
eng; USA
biota
oceans
Bellingham, WA
2016-10-11
0
BCO-DMO catalogue of parameters from Carbon and Nitrogen values for low to high pCO2 acclimated Emiliania huxleyi (E Hux Response to pCO2 project)
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/662346.rdf
Name: expt
Units: unitless
Description: <p>experiment identification</p>
http://lod.bco-dmo.org/id/dataset-parameter/662347.rdf
Name: treatment
Units: unitless
Description: <p>sample name; includes information on capsule material; pCO2 level; replicate id</p>
http://lod.bco-dmo.org/id/dataset-parameter/662348.rdf
Name: N_Peak_Area
Units: relative units
Description: <p>raw Nitrogen (N) peak area</p>
http://lod.bco-dmo.org/id/dataset-parameter/662349.rdf
Name: C_peak_area
Units: relative units
Description: <p>raw Carbon (C) peak area</p>
http://lod.bco-dmo.org/id/dataset-parameter/662350.rdf
Name: N_area_adj
Units: milligrams
Description: <p>mg total particulate nitrogen N calculated from calibration</p>
http://lod.bco-dmo.org/id/dataset-parameter/662351.rdf
Name: C_area_adj
Units: milligrams
Description: <p>mg total particulate nitrogen C calculated from calibration</p>
http://lod.bco-dmo.org/id/dataset-parameter/662352.rdf
Name: mg_N
Units: milligrams
Description: <p>mg total particulate nitrogen N adjusted (filter blank subtracted from value)</p>
http://lod.bco-dmo.org/id/dataset-parameter/662353.rdf
Name: mg_C
Units: milligrams
Description: <p>mg total particulate nitrogen C adjusted (filter blank subtracted from value)</p>
http://lod.bco-dmo.org/id/dataset-parameter/662354.rdf
Name: cells_ml
Units: cells/milliliter
Description: <p>cells/ml in culture</p>
http://lod.bco-dmo.org/id/dataset-parameter/662355.rdf
Name: cells_filter
Units: cells
Description: <p>cells/ml in culture multiplied by the mls filtered per sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/662356.rdf
Name: mg_N_cell
Units: milligrams/cell
Description: <p>total particulate nitrogen</p>
http://lod.bco-dmo.org/id/dataset-parameter/662357.rdf
Name: mg_C_cell
Units: milligrams/cell
Description: <p>total particulate carbon</p>
http://lod.bco-dmo.org/id/dataset-parameter/662358.rdf
Name: pg_N_cell
Units: picograms/cell
Description: <p>picograms (pg) N/cell is total N from tin capsule sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/662359.rdf
Name: pg_TC_cell
Units: picograms/cell
Description: <p>pg TC/cell is total C from tin capsule sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/662360.rdf
Name: pg_PON_cell
Units: picograms
Description: <p>pg PON/cell is particulate organic N from acidified silver capsule</p>
http://lod.bco-dmo.org/id/dataset-parameter/662361.rdf
Name: TC_N
Units: dimensionless
Description: <p>TC:N is Total C per N both from tin capsule sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/662362.rdf
Name: pg_POC_cell
Units: picograms/cell
Description: <p>pg POC/cell is particulate organic C from acidified silver capsule</p>
http://lod.bco-dmo.org/id/dataset-parameter/662363.rdf
Name: pg_PIC_cell
Units: picograms/cell
Description: <p>pg PIC/cell is total C minus organic C; particulate inorganic carbon</p>
http://lod.bco-dmo.org/id/dataset-parameter/662364.rdf
Name: PIC_POC
Units: dimensionless
Description: <p>PIC:POC is the ratio of inorganic C to organic C</p>
http://lod.bco-dmo.org/id/dataset-parameter/662365.rdf
Name: POC_PON
Units: dimensionless
Description: <p>POC:PON is the ratio of organic C to either N or PON depending on whether the row is the tin capsule sample or the acidified silver capsule sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/662366.rdf
Name: PIC_PON
Units: dimensionless
Description: <p>PIC:PON is the ratio of inorganic C to total N.</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
6327
https://datadocs.bco-dmo.org/file/2GGXP4lUpMvKZK/expt2_3_CN.csv
expt2_3_CN.csv
Primary data file for dataset ID 661354
download
https://osprey.bco-dmo.org/dataset/661354/data/download
download
onLine
dataset
<p>The phytoplankton Emiliania huxleyi CCMP 2668 was grown semi-continuously in atmosphere controlled chambers at three different CO2 treatment concentrations; Ambient (400ppmv), Moderate (750ppmv), and High (1000ppmv).&nbsp; Cultures were diluted daily starting day 4 with pre-equilibrated media containing f/50 nutrients.&nbsp;</p>
<p>Expt. 2: On day 8, after ~ 16 generations, 200 mls of E. huxleyi cells from each of the treatment replicates was divided in to two 100 ml samples which were each filtered onto 20mm muffled glass fiber filters (GFF).</p>
<p>Expt. 3:&nbsp;On day 10, after ~ 20 generations, 200 mls of E. huxleyi cells from each of the treatment replicates was divided in to two 100 ml samples which were each filtered onto 20mm muffled glass fiber filters (GFF).</p>
<p>During this sampling, 5 mls of each treatment was fixed in alkaline Lugol’s for counting cells.&nbsp; One filter was used for TPC (total particulate carbon) and placed in a tin capsule the other was used for TOC and placed in a silver capsule.&nbsp; All samples were dried for 24 hours at 60°C.&nbsp; The tin capsules and filters were then folded and held in a desiccator until analysis.&nbsp; The silver capsules and filters were fumed in a closed container with concentrated sulfuric acid for 24 hours to remove the PIC contained in E. huxleyi’s coccoliths.&nbsp; The silver capsules and filters were then dried again at 60°C for 24 hrs, folded, and put in a tin capsule which was folded, then they were held in a desiccator until analysis.&nbsp; For analysis, folded capsules were combusted in a CE Elantech Flash EA 1112 elemental analyzer.&nbsp; Standard curves were made using known weights of Acetanilide wrapped in tin capsules. Media blanks, filter blanks and capsule blanks were included as controls for background signals.&nbsp; Cells were counted with a Hemocytometer or gridded Sedgewick Rafter Chamber, depending on cell density.</p>
Specified by the Principal Investigator(s)
<p>Peak areas are raw data from the elemental analyzer (EA).&nbsp; The values for mg C and mg N are calculated by the EA software based on the values from the standard curve.&nbsp; C and N mg values were then adjusted to remove the respective values from the filter blank samples.</p>
<p><strong>BCO-DMO Processing:</strong><br />
- added conventional header with dataset name, PI name, version date<br />
- column names reformatted to comply with BCO-DMO standards<br />
- added column 'expt' for experiment number<br />
- reduced digits to right of decimal from 9 to 3 for expt3 values of: pg_N_cell, pg_TC_cell, pg_PON_cell, TC_N_, pg_POC_cell, pg_PIC_cell, PIC_POC, POC_PON, PIC_PON<br />
- nd (no data) was entered into all blank cells</p>
<p>&nbsp;</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
PI Supplied Instrument Name: PI Supplied Instrument Description:CE Elantech Flash EA 1112 elemental analyzer Instrument Name: CHN Elemental Analyzer Instrument Short Name:CHN_EA Instrument Description: A CHN Elemental Analyzer is used for the determination of carbon, hydrogen, and nitrogen content in organic and other types of materials, including solids, liquids, volatile, and viscous samples.
Deployment: Lab_Olson_B
Lab_Olson_B
WWU
laboratory
WWU
laboratory