http://lod.bco-dmo.org/id/dataset/661388
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2016-10-11
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Carbonate chemistry data for low-high pCO2 acclimated E. huxleyi cultures (E Hux Response to pCO2 project)
2016-10-11
publication
2016-10-11
revision
BCO-DMO Linked Data URI
2016-10-11
creation
http://lod.bco-dmo.org/id/dataset/661388
M. Brady Olson
Western Washington University
principalInvestigator
Brooke Love
Western Washington University
principalInvestigator
Suzanne Strom
Western Washington University
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Olson, M. Brady, Love, B., Strom, S. (2016) Carbonate chemistry data for low-high pCO2 acclimated E. huxleyi cultures (E Hux Response to pCO2 project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). Version Date 2016-10-11 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/661388 [access date]
Carbonate chemistry data for low-high pCO2 acclimated E. huxleyi cultures Dataset Description: <p><strong>Related Datasets:</strong><br />
<a href="http://www.bco-dmo.org/dataset/661464">Grazing experiments 2 and 3: cell volume</a><br />
<a href="http://www.bco-dmo.org/dataset/661354">Grazing experiments 2 and 3: CN data</a><br />
<a href="http://www.bco-dmo.org/dataset/661405">Grazing experiments 2 and 3: daily cell counts</a><br />
<a href="http://www.bco-dmo.org/dataset/661421">Grazing experiments 2 and 3: ingestion</a></p>
<p><strong>Related Reference:</strong><br />
Kendall, K., Marine Microzooplankton are Indirectly Affected by Ocean Acidification Through Direct Effects on Their Phytoplankton Prey. (Masters Thesis) Western Washington University.<br />
<a href="http://cedar.wwu.edu/wwuet/448/">http://cedar.wwu.edu/wwuet/448/</a></p> Methods and Sampling: <p>The phytoplankton Emiliania huxleyi CCMP 2668 was grown semi-continuously in atmosphere controlled chambers at three different CO2 treatment concentrations; Ambient (400ppmv), Moderate (750ppmv), and High (1000ppmv).&nbsp; Cultures were diluted daily starting day 4 with pre-equilibrated media containing f/50 nutrients.&nbsp; Some of the culture removed was used to evaluate chemical parameters.&nbsp; Samples for pH were filtered through a GFF to remove cells and loose coccoliths both of which increase optical scatter.&nbsp; Samples were warmed to 25°C in a water bath and run within three hours of sampling.&nbsp; pH was analyzed spectrophotometrically with m-cresol dye on an Agilent 8453A UV-VIS Diode Array Spectrophotometer.&nbsp; Samples for total Alkalinity were taken on growth days 5, 7 and 10.&nbsp; Alkalinity was measured by gran titration using a Titrando 888, and 0.1 N HCl titrant, in a temperature controlled titration vessel (+/1 5 ueq/kg).&nbsp; Other parameters were calculated with CO2sys</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-0961229 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=0961229
completed
M. Brady Olson
Western Washington University
(360) 650-7400
1900 Shannon Point Rd.
Anacortes
WA
98221
United States
Brady.Olson@wwu.edu
pointOfContact
Brooke Love
Western Washington University
(360) 650-2894
516 High St. Mail Stop 9181
Bellingham
WA
98225
United States
brooke.love@wwu.edu
pointOfContact
Suzanne Strom
Western Washington University
360-293-2188
Shannon Point Marine Center 1900 Shannon Point Rd
Anacortes
WA
98221
USA
suzanne.strom@wwu.edu
pointOfContact
asNeeded
Unknown
treatment_rep_culture_day
pH
pCO2
total_alkalinity
theme
None, User defined
treatment
pH
Partial pressure of CO2
total alkalinity (TA)
featureType
BCO-DMO Standard Parameters
Spectrophotometer
Titrator
instrument
BCO-DMO Standard Instruments
Lab_Olson_B
service
Deployment Activity
Bellingham, WA
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Planktonic interactions in a changing ocean: Biological responses of Emiliania huxleyi to elevated pCO2 and their effects on microzooplankton
https://osprey.bco-dmo.org/project/517694
Planktonic interactions in a changing ocean: Biological responses of Emiliania huxleyi to elevated pCO2 and their effects on microzooplankton
<p><em><strong>Description from NSF award abstract:</strong></em><br />
The calcifying Haptophyte <em>Emiliania huxleyi </em>appears to be acutely sensitive to the rising concentration of ocean pCO2. Documented responses by <em>E. huxleyi </em>to elevated pCO2 include modifications to their calcification rate and cell size, malformation of coccoliths, elevated growth rates, increased organic carbon production, lowering of PIC:POC ratios, and elevated production of the active climate gas DMS. Changes in these parameters are mechanisms known to elicit alterations in grazing behavior by microzooplankton, the oceans dominant grazer functional group. The investigators hypothesize that modifications to the physiology and biochemistry of calcifying and non-calcifying Haptophyte <em>Emiliania huxleyi </em>in response to elevated pCO2 will precipitate alterations in microzooplankton grazing dynamics. To test this hypothesis, they will conduct controlled laboratory experiments where several strains of <em>E. huxleyi</em> are grown at several CO2 concentrations. After careful characterization of the biochemical and physiological responses of the <em>E. huxleyi</em> strains to elevated pCO2, they will provide these strains as food to several ecologically-important microzooplankton and document grazing dynamics.<em> E. huxleyi</em> is an ideal organism for the study of phytoplankton and microzooplankton responses to rising anthropogenic CO2, the effects of which in the marine environment are called ocean acidification; <em>E. huxleyi </em>is biogeochemically important, is well studied, numerous strains are in culture that exhibit variation in the parameters described above, and they are readily fed upon by ecologically important microzooplankton.</p>
<p>The implications of changes in microzooplankton grazing for carbon cycling, specifically CaCO3 export, DMS production, nutrient regeneration in surface waters, and carbon transfer between trophic levels are profound, as this grazing, to a large degree, regulates all these processes. <em>E. huxleyi </em>is a model prey organism because it is one of the most biogeochemically influential global phytoplankton. It forms massive seasonal blooms, contributes significantly to marine inorganic and organic carbon cycles, is a large producer of the climatically active gas DMS, and is a source of organic matter for trophic levels both above and below itself. The planned controlled study will increase our knowledge of the mechanisms that drive patterns of change between trophic levels, thus providing a wider array of tools necessary to understand the complex nature of ocean acidification field studies, where competing variables can confound precise interpretation.</p>
E Hux Response to pCO2
largerWorkCitation
project
eng; USA
biota
oceans
Bellingham, WA
2016-10-11
0
BCO-DMO catalogue of parameters from Carbonate chemistry data for low-high pCO2 acclimated E. huxleyi cultures (E Hux Response to pCO2 project)
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/661400.rdf
Name: treatment_rep_culture_day
Units: unitless
Description: <p>pCO2 level (ambient; moderate; high) replicate (A;B;C) and day of semi-continuous culture</p>
http://lod.bco-dmo.org/id/dataset-parameter/661401.rdf
Name: pH
Units: pH scale
Description: <p>pH of the culture material removed</p>
http://lod.bco-dmo.org/id/dataset-parameter/661402.rdf
Name: pCO2
Units: parts per million by volume (ppmv)
Description: <p>Partial pressure of carbon dioxide (pCO2) of the culture material removed; computation from pH and alkalinity</p>
http://lod.bco-dmo.org/id/dataset-parameter/661403.rdf
Name: total_alkalinity
Units: micromoles per kilogram (umol/kg)
Description: <p>total alkalinity of the culture material removed</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2055
https://datadocs.bco-dmo.org/file/rggprZWT2XJmp/expt2_3_chemistry.csv
expt2_3_chemistry.csv
Primary data file for dataset ID 661388
download
https://osprey.bco-dmo.org/dataset/661388/data/download
download
onLine
dataset
<p>The phytoplankton Emiliania huxleyi CCMP 2668 was grown semi-continuously in atmosphere controlled chambers at three different CO2 treatment concentrations; Ambient (400ppmv), Moderate (750ppmv), and High (1000ppmv).&nbsp; Cultures were diluted daily starting day 4 with pre-equilibrated media containing f/50 nutrients.&nbsp; Some of the culture removed was used to evaluate chemical parameters.&nbsp; Samples for pH were filtered through a GFF to remove cells and loose coccoliths both of which increase optical scatter.&nbsp; Samples were warmed to 25°C in a water bath and run within three hours of sampling.&nbsp; pH was analyzed spectrophotometrically with m-cresol dye on an Agilent 8453A UV-VIS Diode Array Spectrophotometer.&nbsp; Samples for total Alkalinity were taken on growth days 5, 7 and 10.&nbsp; Alkalinity was measured by gran titration using a Titrando 888, and 0.1 N HCl titrant, in a temperature controlled titration vessel (+/1 5 ueq/kg).&nbsp; Other parameters were calculated with CO2sys</p>
Specified by the Principal Investigator(s)
<p><strong>BCO-DMO Processing:</strong><br />
- added conventional header with dataset name, PI name, version date<br />
- column names reformatted to comply with BCO-DMO standards<br />
- nd (no data) was entered into all blank cells</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
PI Supplied Instrument Name: PI Supplied Instrument Description:Agilent 8453A UV-VIS Diode Array Spectrophotometer Instrument Name: Spectrophotometer Instrument Short Name:Spectrophotometer Instrument Description: An instrument used to measure the relative absorption of electromagnetic radiation of different wavelengths in the near infra-red, visible and ultraviolet wavebands by samples. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB20/
PI Supplied Instrument Name: PI Supplied Instrument Description:Titrando 888 Instrument Name: Titrator Instrument Short Name:Titrator Instrument Description: Titrators are instruments that incrementally add quantified aliquots of a reagent to a sample until the end-point of a chemical reaction is reached. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB12/
Deployment: Lab_Olson_B
Lab_Olson_B
WWU
laboratory
WWU
laboratory